FastQCFastQC Report
Mon 15 Aug 2016
SRR2921583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711343
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.13591664558186173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19630.11470523442699682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26400.056.7110181
GTATCAA42100.045.6216961
GTACATG87350.035.9869771
TCAACGC53850.035.5469634
CAACGCA54700.034.9587445
TACATGG90250.034.56322
TATCAAC56050.034.157722
ACATGGG91250.033.4654433
ATCAACG57200.033.3667143
AACGCAG57800.033.0010076
CATGGGG79650.032.8671384
ATGGGGG44200.031.67265
ACGCAGA69250.027.5445237
CGCAGAG73300.026.0203368
GTATAGG4300.024.9690321
GCAGAGT82950.022.849559
ATGGGGA32350.021.7293055
AGAGTAC74750.021.64838810-11
GAGTACT34450.021.60506612-13
CAGAGTA78750.021.49486410-11
Produced by FastQC (version 0.11.5)