##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921583_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1711343 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.711422549424633 33.0 31.0 34.0 30.0 34.0 2 31.874156729539315 33.0 31.0 34.0 30.0 34.0 3 31.93931958701441 34.0 31.0 34.0 30.0 34.0 4 35.338074249288425 37.0 35.0 37.0 33.0 37.0 5 35.17658061534129 37.0 35.0 37.0 33.0 37.0 6 35.198978229378916 37.0 35.0 37.0 33.0 37.0 7 35.07949195456434 37.0 35.0 37.0 33.0 37.0 8 35.11566705213391 37.0 35.0 37.0 33.0 37.0 9 36.8394898042064 39.0 38.0 39.0 34.0 39.0 10-11 36.79122274143757 39.0 38.0 39.0 33.0 39.0 12-13 36.72491662980478 39.0 38.0 39.0 33.0 39.0 14-15 38.15867362650269 41.0 39.0 41.0 33.0 41.0 16-17 38.15826868138065 41.0 39.0 41.0 33.0 41.0 18-19 38.164015045493514 41.0 39.0 41.0 33.5 41.0 20-21 38.09767971704095 41.0 39.0 41.0 33.0 41.0 22-23 38.101856553595624 41.0 39.0 41.0 34.0 41.0 24-25 38.062015621649195 41.0 39.0 41.0 33.5 41.0 26-27 37.98111132601705 41.0 39.0 41.0 33.0 41.0 28-29 37.90692952844637 41.0 39.0 41.0 33.0 41.0 30-31 37.796254462138805 41.0 38.0 41.0 33.0 41.0 32-33 37.67809579961469 41.0 38.0 41.0 33.0 41.0 34-35 37.53826439235151 41.0 38.0 41.0 32.0 41.0 36-37 37.45674800434512 40.5 38.0 41.0 32.0 41.0 38-39 37.366855738446354 40.0 38.0 41.0 32.0 41.0 40-41 37.242799660851155 40.0 38.0 41.0 32.0 41.0 42-43 37.07736613875769 40.0 37.5 41.0 31.0 41.0 44-45 36.916752515422104 40.0 37.0 41.0 31.0 41.0 46-47 36.713810440104645 40.0 37.0 41.0 30.5 41.0 48-49 36.521435504162525 40.0 36.0 41.0 30.0 41.0 50-51 36.27159663492357 40.0 36.0 41.0 30.0 41.0 52-53 36.12674577802346 40.0 35.0 41.0 29.0 41.0 54-55 35.9829931813786 39.5 35.0 41.0 29.0 41.0 56-57 35.77597156151631 39.0 35.0 41.0 29.0 41.0 58-59 35.572747836056244 39.0 35.0 41.0 29.0 41.0 60-61 35.310140924408486 38.5 35.0 41.0 28.0 41.0 62-63 34.95799789989499 37.5 35.0 40.5 28.0 41.0 64-65 34.668442270193644 37.0 35.0 40.0 28.0 41.0 66-67 34.362541582838745 36.5 35.0 39.5 28.0 41.0 68-69 34.002120848947285 36.0 35.0 39.0 27.5 41.0 70-71 33.63453936469779 35.0 34.0 39.0 26.0 40.5 72-73 33.33032916253492 35.0 34.0 37.0 26.0 39.5 74-75 33.01949141697486 35.0 34.0 37.0 26.0 39.0 76-77 32.70758492014751 35.0 34.0 36.5 26.0 39.0 78-79 32.45500200719552 35.0 34.0 36.0 26.0 37.5 80-81 32.21559500345634 35.0 34.0 36.0 26.0 37.0 82-83 32.00733692778128 35.0 34.0 35.0 25.5 37.0 84-85 31.80942657316505 35.0 34.0 35.0 25.0 36.0 86-87 31.690373583787704 35.0 34.0 35.0 25.0 36.0 88-89 31.59380965709387 35.0 33.5 35.0 25.0 36.0 90-91 31.495170751859796 35.0 33.0 35.0 24.0 35.5 92-93 31.420106021995593 35.0 33.0 35.0 24.0 35.0 94-95 31.36260469116945 35.0 33.0 35.0 24.0 35.0 96-97 31.297385445232194 35.0 33.0 35.0 24.0 35.0 98-99 31.218859690897734 35.0 33.0 35.0 23.0 35.0 100-101 31.152090492671547 35.0 33.0 35.0 23.0 35.0 102-103 30.45221998161678 34.0 31.5 35.0 20.0 35.0 104-105 30.973785792795482 35.0 33.0 35.0 20.0 35.0 106-107 31.076941910534593 35.0 33.0 35.0 22.5 35.0 108-109 31.060820069384103 35.0 33.0 35.0 21.0 35.0 110-111 30.9893899118996 35.0 33.0 35.0 20.0 35.0 112-113 30.94569557359337 35.0 33.0 35.0 19.5 35.0 114-115 30.819312376303287 35.0 33.0 35.0 19.0 35.0 116-117 31.011448026491475 35.0 33.0 35.0 19.5 35.0 118-119 30.991727257481404 35.0 33.0 35.0 18.5 35.0 120-121 30.90477303497896 35.0 33.0 35.0 17.5 35.0 122-123 30.76267936936079 35.0 33.0 35.0 4.5 35.0 124-125 30.10813700117393 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29837.0 3 8086.0 4 4360.0 5 3404.0 6 3672.0 7 3980.0 8 4387.0 9 4562.0 10 4782.0 11 5119.0 12 5654.0 13 6936.0 14 8898.0 15 8079.0 16 4917.0 17 4241.0 18 3818.0 19 3644.0 20 3546.0 21 3632.0 22 3969.0 23 4969.0 24 6590.0 25 8492.0 26 10059.0 27 12427.0 28 14619.0 29 17737.0 30 21600.0 31 26848.0 32 35283.0 33 48275.0 34 73825.0 35 135340.0 36 317766.0 37 630437.0 38 217249.0 39 304.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.125288371448725 17.39638938781379 11.01577472922162 27.46254751151586 2 16.37510452586018 19.3133987690053 36.56917931933578 27.742317385798742 3 19.127623483310128 22.725652005672206 28.205843121163337 29.940881389854322 4 13.769930856222734 14.706968636832157 33.204172858857845 38.31892764808727 5 15.148337775284052 36.81441997475102 32.30882077897788 15.728421470987048 6 35.61968002217309 32.97791286419391 16.051458880668186 15.350948232964814 7 31.07341527938146 31.135912538914628 18.815592160072494 18.97508002163142 8 28.689614695031445 33.643318588183575 18.241291602630394 19.425775114154582 9 28.059177166171285 14.889276622856626 18.221762397014256 38.82978381395784 10-11 27.283618637660872 24.468142332332913 25.683938117698922 22.56430091230729 12-13 27.208661645202763 21.912065720645163 26.331026063631064 24.54824657052101 14-15 24.594331338072227 23.264865357878282 23.677316114608047 28.46348718944145 16-17 24.84644491440581 26.392913359358218 24.398955302192142 24.361686424043835 18-19 24.810508749009948 25.393493554053304 26.16724097114449 23.628756725792265 20-21 25.534746426670885 24.350216791658788 26.090833031117644 24.024203750552683 22-23 25.42502725994594 23.92803841753238 25.865013890008893 24.781920432512788 24-25 24.9016813224315 24.493417761758504 25.891062320893464 24.713838594916528 26-27 24.57226069052445 24.762321600590433 26.144872285204144 24.520545423680975 28-29 24.610071437962922 24.923697243283517 26.043476355349082 24.42275496340448 30-31 25.019918968438475 24.58674166580719 25.878384873329384 24.51495449242495 32-33 24.723994857410005 24.561272791023843 25.866643291257596 24.848089060308556 34-35 24.830155977177267 24.726695167816295 25.97947379616146 24.463675058844977 36-37 24.88495918604077 24.693752865246 25.955001649388265 24.466286299324963 38-39 24.80888162479179 24.838654164342667 26.006473993438355 24.34599021742719 40-41 24.92596254534575 24.611376407120687 25.96815512921201 24.49450591832155 42-43 24.793123296236455 24.516167675168077 25.8187268631467 24.871982165448774 44-45 24.76954236885025 24.64437210047047 25.761058021853955 24.825027508825322 46-47 24.785422773235346 24.779696038969842 25.62853830367119 24.806342884123623 48-49 24.639187757934334 25.024359957634857 25.571863701106608 24.764588583324205 50-51 24.687091598987568 25.016368909794085 25.59939719170572 24.697142299512628 52-53 24.942040744297643 24.860729788471726 25.350874450683573 24.846355016547054 54-55 24.637242040245084 24.736963591261436 25.488688192652802 25.137106175840678 56-57 24.666451824584847 24.979657688195967 25.30072291536225 25.05316757185693 58-59 24.613189297592026 24.960579010123386 25.538928830103057 24.88730286218153 60-61 24.80271296335793 24.85699794137807 25.654326816603838 24.68596227866016 62-63 24.665587383784988 24.865228421427535 25.59530748605683 24.87387670873065 64-65 24.584522214424577 24.627120337652944 25.605650065474894 25.18270738244759 66-67 24.587004566178024 24.96414960047403 25.390994332890386 25.057851500457552 68-69 24.49093001560481 24.977364116771064 25.481532946714296 25.05017292090983 70-71 24.3953140895776 24.773146002876103 25.666567134700642 25.164972772845655 72-73 24.58831603794671 24.95201731788421 25.430164124293043 25.029502519876033 74-75 24.38823711251949 25.01040897559026 25.45426230384742 25.14709160804283 76-77 24.473299464621924 24.74683196654734 25.33623285966222 25.44363570916851 78-79 24.311337041730127 24.93269169245297 25.468557853590294 25.287413412226606 80-81 24.371940633759568 25.07776056582462 25.379073628138837 25.17122517227698 82-83 24.426510913757866 24.898707529404547 25.494643558903324 25.180137997934267 84-85 24.32579782027781 24.90429896471669 25.315969728612842 25.45393348639266 86-87 24.139579266108548 25.106159314643527 25.369928763550032 25.384332655697893 88-89 24.15598629593465 24.951572385478553 25.314129410272905 25.57831190831389 90-91 24.082652504611172 24.909493094128056 25.456697949289254 25.55115645197152 92-93 24.148987082075305 24.883381063877902 25.605650065474894 25.361981788571896 94-95 24.11257419806486 24.725267019537217 25.603985980232547 25.558172802165373 96-97 24.01140871044875 24.698654375208903 25.55477513317174 25.7351617811706 98-99 23.900598920313122 24.82052696456176 25.41462646439508 25.864247650730032 100-101 23.879403486034068 24.855946470111487 25.53824686225964 25.726403181594804 102-103 23.665833684670005 24.713006878017953 25.753021860591364 25.868137576720677 104-105 23.709653763155604 24.830673921008238 25.600946157491517 25.85872615834465 106-107 23.78092320853415 24.74823763422262 25.535458846188007 25.935380311055223 108-109 23.7035632435111 24.749810963089008 25.5379517164084 26.00867407699149 110-111 23.556674494826577 25.008604353423014 25.51145503852822 25.923266113222187 112-113 23.443519144247784 25.052378652591116 25.560022824266298 25.944079378894802 114-115 23.336671841939342 25.097160534153584 25.56004260980996 26.006125014097115 116-117 23.227785117795104 24.98397434960986 25.631924848610925 26.15631568398411 118-119 23.32071334950421 24.88960192414336 25.49507481708425 26.29460990926818 120-121 23.189732019595624 24.985997491381553 25.54513365712217 26.27913683190065 122-123 22.95742081154471 25.12287234073635 25.52411020908312 26.395596638635823 124-125 22.81589446508517 25.086711998589095 25.54613423447322 26.55125930185252 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 50.0 1 37.0 2 29.0 3 44.5 4 52.5 5 70.0 6 155.5 7 369.5 8 637.0 9 712.5 10 562.5 11 421.0 12 400.0 13 433.0 14 480.0 15 535.5 16 568.0 17 611.5 18 657.0 19 684.5 20 733.5 21 803.5 22 920.5 23 1042.5 24 1186.0 25 1420.0 26 1792.5 27 2393.5 28 3300.0 29 4475.5 30 6042.0 31 8037.5 32 10647.0 33 12928.5 34 14914.5 35 17519.5 36 20882.5 37 24661.0 38 27974.0 39 31403.5 40 34932.5 41 38364.0 42 42242.5 43 47184.5 44 52077.0 45 56679.0 46 61682.0 47 66044.5 48 70364.5 49 74637.5 50 76984.5 51 77818.0 52 77592.5 53 77246.0 54 77919.5 55 76617.5 56 72278.0 57 66456.0 58 61159.5 59 55997.5 60 48819.0 61 41753.0 62 35376.5 63 27792.0 64 20893.0 65 16211.5 66 12583.0 67 9928.5 68 7932.5 69 6028.0 70 4668.0 71 3648.5 72 2735.5 73 2337.5 74 1921.5 75 1402.5 76 1010.5 77 601.5 78 334.5 79 198.0 80 125.5 81 67.5 82 46.5 83 28.0 84 15.5 85 13.0 86 10.0 87 7.0 88 3.5 89 3.5 90 2.5 91 0.5 92 1.5 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025827668678926434 2 0.002571080139983627 3 0.03184633355206992 4 0.16793828005256692 5 0.02238008394576657 6 0.06789988915138578 7 0.05072039912513155 8 0.04230595503063968 9 0.03202163447070517 10-11 0.062173392475967704 12-13 0.05562882484691847 14-15 0.05592099304464388 16-17 0.046776128455838485 18-19 0.033774643657057644 20-21 0.021532796172362877 22-23 0.0023957792213483795 24-25 0.004148788407700853 26-27 0.00289246515748158 28-29 0.003973487489065605 30-31 0.0020159605643053436 32-33 0.008356010454946787 34-35 0.003915053849520523 36-37 0.005989448053370949 38-39 0.0020159605643053436 40-41 0.002746381058618874 42-43 0.003389151093614781 44-45 0.004324089326336099 46-47 0.004148788407700853 48-49 0.001782226006125014 50-51 0.0011394559711291072 52-53 6.719868547684479E-4 54-55 0.004557823884516429 56-57 4.966859361332007E-4 58-59 4.966859361332007E-4 60-61 2.3373455818032974E-4 62-63 0.0012563232502192721 64-65 0.0 66-67 0.003798186570430358 68-69 6.719868547684479E-4 70-71 0.0 72-73 0.002834031517936498 74-75 0.004645474343834053 76-77 0.0016945755468073904 78-79 8.765045931762365E-5 80-81 0.0 82-83 0.0049960761811045475 84-85 0.0014024073490819784 86-87 0.0 88-89 0.0015192746281721431 90-91 5.551195756782831E-4 92-93 0.0 94-95 0.0026295137795287094 96-97 0.0011102391513565662 98-99 7.888541338586127E-4 100-101 0.0 102-103 0.0012563232502192721 104-105 0.0 106-107 4.09035476815577E-4 108-109 0.0021620446631680497 110-111 0.0 112-113 3.798186570430358E-4 114-115 0.0 116-117 0.0014024073490819784 118-119 0.0021036110236229673 120-121 0.0024834296806660033 122-123 8.18070953631154E-4 124-125 0.10544350255910125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1711343.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.433754499923175 #Duplication Level Percentage of deduplicated Percentage of total 1 78.5390707545434 47.46410920632172 2 11.774839972716816 14.231955763741 3 3.8665922285234315 7.010180564696876 4 1.8218630543642584 4.404080982397192 5 1.0107282967696922 3.0541052876552532 6 0.6374272164572514 2.311327194656815 7 0.4473484498571532 1.8924462476211872 8 0.3265759959444812 1.5788970851581348 9 0.2506158345928698 1.3631090239420962 >10 1.2641000023394269 13.190443332575441 >50 0.047836134131430945 1.9105502832011079 >100 0.012517009073839255 1.189956443946234 >500 1.9402027441228862E-4 0.07158340602238306 >1k 2.910304116184329E-4 0.3272551780646537 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2326 0.13591664558186173 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1963 0.11470523442699682 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.843363954508243E-5 2 0.0 0.0 0.0 0.0 5.843363954508243E-5 3 0.0 0.0 0.0 0.0 5.843363954508243E-5 4 0.0 0.0 0.0 0.0 5.843363954508243E-5 5 0.0 0.0 0.0 0.0 5.843363954508243E-5 6 0.0 0.0 0.0 0.0 1.1686727909016486E-4 7 0.0 0.0 0.0 0.0 1.1686727909016486E-4 8 0.0 0.0 0.0 0.0 1.1686727909016486E-4 9 0.0 0.0 0.0 0.0 1.1686727909016486E-4 10-11 0.0 0.0 0.0 0.0 1.1686727909016486E-4 12-13 0.0 0.0 0.0 2.9216819772541214E-5 2.045177384077885E-4 14-15 0.0 0.0 0.0 5.843363954508243E-5 2.337345581803297E-4 16-17 0.0 0.0 0.0 5.843363954508243E-5 2.9216819772541215E-4 18-19 0.0 0.0 0.0 8.765045931762364E-5 4.09035476815577E-4 20-21 0.0 0.0 0.0 1.4608409886270607E-4 4.382522965881182E-4 22-23 0.0 0.0 0.0 3.213850174979534E-4 6.135532152233655E-4 24-25 0.0 0.0 0.0 8.472877734036952E-4 7.888541338586129E-4 26-27 0.0 0.0 0.0 0.0015484914479446843 8.18070953631154E-4 28-29 0.0 0.0 0.0 0.004294872506563558 8.472877734036952E-4 30-31 0.0 0.0 0.0 0.017150273206481693 8.765045931762365E-4 32-33 0.0 0.0 0.0 0.04195535319336918 8.765045931762365E-4 34-35 0.0 0.0 0.0 0.07476584179793297 9.349382327213188E-4 36-37 0.0 0.0 0.0 0.11853263781719972 9.349382327213188E-4 38-39 0.0 0.0 0.0 0.17918675566499526 9.349382327213188E-4 40-41 5.843363954508243E-5 0.0 0.0 0.2469113438977458 9.933718722664013E-4 42-43 5.843363954508243E-5 0.0 0.0 0.32001182696864394 0.0010518055118114837 44-45 5.843363954508243E-5 0.0 0.0 0.40999963186807087 0.0011686727909016486 46-47 5.843363954508243E-5 0.0 0.0 0.5092199518156209 0.001227106430446731 48-49 5.843363954508243E-5 0.0 0.0 0.6015451022968511 0.0012855400699918135 50-51 5.843363954508243E-5 0.0 0.0 0.6908024867019644 0.0012855400699918135 52-53 5.843363954508243E-5 0.0 0.0 0.7879776292654366 0.0014316241688545196 54-55 5.843363954508243E-5 0.0 0.0 0.9060720147860482 0.0015777082677172255 56-57 5.843363954508243E-5 0.0 0.0 1.0447058246067562 0.0015777082677172255 58-59 5.843363954508243E-5 0.0 0.0 1.1798043992349867 0.0016069250874897667 60-61 5.843363954508243E-5 0.0 0.0 1.305056905599871 0.001636141907262308 62-63 5.843363954508243E-5 0.0 0.0 1.4320916379708801 0.0018698764654426377 64-65 5.843363954508243E-5 0.0 0.0 1.5810389851712952 0.0018698764654426377 66-67 5.843363954508243E-5 0.0 0.0 1.7639070601276308 0.0018990932852151789 68-69 5.843363954508243E-5 0.0 0.0 1.95475132688187 0.0019575269247602617 70-71 5.843363954508243E-5 0.0 0.0 2.1439010180893017 0.0019867437445328026 72-73 5.843363954508243E-5 0.0 0.0 2.327470296720178 0.0022204783027131325 74-75 5.843363954508243E-5 2.9216819772541214E-5 0.0 2.528160631737764 0.0022204783027131325 76-77 5.843363954508243E-5 5.843363954508243E-5 0.0 2.78036022001434 0.0022204783027131325 78-79 5.843363954508243E-5 5.843363954508243E-5 0.0 3.0555826622716777 0.0022204783027131325 80-81 5.843363954508243E-5 8.765045931762364E-5 0.0 3.3190015093409095 0.0023081287620307558 82-83 5.843363954508243E-5 1.1686727909016486E-4 0.0 3.5809887322412868 0.002454212860893462 84-85 5.843363954508243E-5 1.1686727909016486E-4 0.0 3.865998809122426 0.002454212860893462 86-87 5.843363954508243E-5 1.1686727909016486E-4 0.0 4.194132911987836 0.002541863320211086 88-89 5.843363954508243E-5 1.1686727909016486E-4 0.0 4.568897059210222 0.002571080139983627 90-91 5.843363954508243E-5 1.1686727909016486E-4 0.0 4.945034396961918 0.002571080139983627 92-93 5.843363954508243E-5 1.1686727909016486E-4 0.0 5.3100109095605035 0.002571080139983627 94-95 5.843363954508243E-5 1.1686727909016486E-4 0.0 5.691085889853758 0.0026587305993012508 96-97 5.843363954508243E-5 1.1686727909016486E-4 0.0 6.1189954322423965 0.0026879474190737917 98-99 5.843363954508243E-5 1.1686727909016486E-4 0.0 6.62260575466169 0.0027171642388463327 100-101 5.843363954508243E-5 1.1686727909016486E-4 0.0 7.1428696643513305 0.002746381058618874 102-103 5.843363954508243E-5 1.1686727909016486E-4 0.0 7.648320646416295 0.0027755978783914155 104-105 5.843363954508243E-5 1.1686727909016486E-4 0.0 8.168584556105936 0.002834031517936498 106-107 8.765045931762364E-5 1.1686727909016486E-4 0.0 8.735040257855964 0.0029216819772541216 108-109 1.1686727909016486E-4 1.1686727909016486E-4 0.0 9.370184702891237 0.003009332436571745 110-111 1.1686727909016486E-4 1.1686727909016486E-4 0.0 10.050060098998273 0.0032138501749795338 112-113 1.1686727909016486E-4 1.1686727909016486E-4 0.0 10.70317288819366 0.0032138501749795338 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2640 0.0 56.711018 1 GTATCAA 4210 0.0 45.621696 1 GTACATG 8735 0.0 35.986977 1 TCAACGC 5385 0.0 35.546963 4 CAACGCA 5470 0.0 34.958744 5 TACATGG 9025 0.0 34.5632 2 TATCAAC 5605 0.0 34.15772 2 ACATGGG 9125 0.0 33.465443 3 ATCAACG 5720 0.0 33.366714 3 AACGCAG 5780 0.0 33.001007 6 CATGGGG 7965 0.0 32.867138 4 ATGGGGG 4420 0.0 31.6726 5 ACGCAGA 6925 0.0 27.544523 7 CGCAGAG 7330 0.0 26.020336 8 GTATAGG 430 0.0 24.969032 1 GCAGAGT 8295 0.0 22.84955 9 ATGGGGA 3235 0.0 21.729305 5 AGAGTAC 7475 0.0 21.648388 10-11 GAGTACT 3445 0.0 21.605066 12-13 CAGAGTA 7875 0.0 21.494864 10-11 >>END_MODULE