FastQCFastQC Report
Mon 15 Aug 2016
SRR2921582_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921582_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233692
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG120900.038.605441
TACATGG125550.037.1697772
GTATCAA42500.037.120661
CATGGGG109300.036.050314
ACATGGG129650.035.120453
ATGGGGG66200.033.9833375
GGTATCA28100.033.686011
TCAACGC48650.032.0545434
CAACGCA49800.031.1920095
CGCCTTA9050.030.9129072
ATCAACG52350.029.675293
AACGCAG52250.029.4969796
TATCAAC53350.029.0087852
ACGCAGA62100.024.5247967
GAGTACA85200.023.6874791
AGTACAT84150.022.8475172
ATGGGGA39000.022.7380855
CGCAGAG67250.022.6446578
TGGGGGG53550.021.6690166
TATACCG2204.170739E-621.6421055
Produced by FastQC (version 0.11.5)