##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921582_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2233692 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.077669168354454 31.0 31.0 34.0 27.0 34.0 2 30.38443930497132 31.0 31.0 34.0 27.0 34.0 3 30.448536772303434 31.0 31.0 34.0 27.0 34.0 4 34.02131985967627 37.0 35.0 37.0 30.0 37.0 5 33.93706562946011 37.0 35.0 37.0 30.0 37.0 6 33.999620359476594 37.0 35.0 37.0 30.0 37.0 7 33.885070546879334 37.0 35.0 37.0 30.0 37.0 8 33.95945278041914 37.0 35.0 37.0 30.0 37.0 9 35.61577916740536 39.0 37.0 39.0 30.0 39.0 10-11 35.63342663178272 39.0 37.0 39.0 30.0 39.0 12-13 35.567689726246954 39.0 37.0 39.0 30.0 39.0 14-15 36.877923858795214 40.5 38.0 41.0 30.0 41.0 16-17 36.84082407064179 40.5 38.0 41.0 30.0 41.0 18-19 36.79362418811546 40.0 38.0 41.0 30.0 41.0 20-21 36.83258211069386 40.5 38.0 41.0 30.0 41.0 22-23 36.7536607553772 40.0 38.0 41.0 30.0 41.0 24-25 36.7227762377266 40.0 38.0 41.0 30.0 41.0 26-27 36.674786183592005 40.0 38.0 41.0 30.0 41.0 28-29 36.613185031776986 40.0 38.0 41.0 30.0 41.0 30-31 36.520874856515576 40.0 38.0 41.0 29.5 41.0 32-33 36.43159956699491 40.0 38.0 41.0 29.0 41.0 34-35 36.30784593399627 40.0 37.0 41.0 28.5 41.0 36-37 36.21099395082223 40.0 37.0 41.0 28.0 41.0 38-39 36.09840591272207 40.0 37.0 41.0 27.0 41.0 40-41 35.985089707981224 40.0 37.0 41.0 27.0 41.0 42-43 35.74711710477541 40.0 36.0 41.0 26.5 41.0 44-45 35.4063337738596 40.0 35.0 41.0 25.5 41.0 46-47 35.15818384987725 39.5 35.0 41.0 24.0 41.0 48-49 34.96464105167588 39.0 35.0 41.0 24.0 41.0 50-51 34.75121793873103 39.0 35.0 41.0 23.5 41.0 52-53 34.543568226953404 39.0 34.5 40.0 23.0 41.0 54-55 34.29678129303413 38.0 34.0 40.0 22.5 41.0 56-57 34.04088030041743 38.0 34.0 40.0 22.0 41.0 58-59 34.0307578215797 38.0 34.0 40.0 22.0 41.0 60-61 33.80604174613152 37.0 34.0 40.0 22.0 41.0 62-63 33.48550068675538 37.0 34.0 40.0 20.5 41.0 64-65 33.18146212638089 36.0 33.5 39.5 20.0 41.0 66-67 32.85285236281457 36.0 33.0 39.0 19.0 41.0 68-69 32.51182817505726 35.0 33.0 39.0 18.0 40.5 70-71 32.14664376288226 35.0 33.0 37.5 16.5 40.0 72-73 31.80424807896523 35.0 33.0 37.0 14.0 39.0 74-75 31.444113154365063 35.0 32.0 36.5 11.5 39.0 76-77 31.159527365455936 35.0 32.0 36.0 10.0 38.5 78-79 30.896910138013656 35.0 32.0 36.0 7.5 37.0 80-81 30.655502190991417 35.0 32.0 35.0 7.0 37.0 82-83 30.445941069762526 35.0 32.0 35.0 6.5 36.5 84-85 30.249463668222837 35.0 31.0 35.0 3.5 36.0 86-87 30.08726516457954 35.0 31.0 35.0 2.0 36.0 88-89 29.935469840962853 35.0 31.0 35.0 2.0 35.5 90-91 29.76138630572165 34.5 31.0 35.0 2.0 35.0 92-93 29.690515970867963 34.0 31.0 35.0 2.0 35.0 94-95 29.57310900518066 34.0 31.0 35.0 2.0 35.0 96-97 29.486475082509138 34.0 31.0 35.0 2.0 35.0 98-99 29.398495181967792 34.0 31.0 35.0 2.0 35.0 100-101 29.293792519290932 34.0 30.0 35.0 2.0 35.0 102-103 28.417415203170357 33.5 29.0 34.5 2.0 35.0 104-105 29.344449234719917 34.0 30.5 35.0 2.0 35.0 106-107 29.62185923574065 35.0 31.0 35.0 2.0 35.0 108-109 29.619943125551778 35.0 31.0 35.0 2.0 35.0 110-111 29.556215225733897 35.0 31.0 35.0 2.0 35.0 112-113 29.4777406643351 35.0 31.0 35.0 2.0 35.0 114-115 29.36260975103103 35.0 31.0 35.0 2.0 35.0 116-117 29.240145463206208 35.0 31.0 35.0 2.0 35.0 118-119 28.94928709956431 35.0 31.0 35.0 2.0 35.0 120-121 28.82508712033709 35.0 31.0 35.0 2.0 35.0 122-123 28.574958633509006 34.0 30.0 35.0 2.0 35.0 124-125 27.797254500620497 34.0 28.5 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 86070.0 3 15862.0 4 7681.0 5 6494.0 6 6931.0 7 7449.0 8 7668.0 9 8032.0 10 8689.0 11 9035.0 12 8663.0 13 8701.0 14 8327.0 15 7518.0 16 6597.0 17 6158.0 18 5963.0 19 5657.0 20 5814.0 21 6146.0 22 7062.0 23 9192.0 24 12516.0 25 15724.0 26 18415.0 27 21459.0 28 25743.0 29 31236.0 30 37540.0 31 46928.0 32 62470.0 33 85362.0 34 134973.0 35 223041.0 36 446057.0 37 661448.0 38 161004.0 39 67.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.10021655761167 16.77166158260804 11.571547303239766 25.556574556540525 2 16.97778669279084 18.82695541757185 37.92689828586984 26.268359603767465 3 20.752384530004804 22.80641820602035 27.709440826986302 28.731756436988547 4 13.991139209334499 14.185548269817568 33.74170280308135 38.08160971776658 5 15.105020917317155 36.934971613971506 32.20393701386771 15.756070454843623 6 35.42285248760745 32.44595089940679 17.034372924678546 15.096823688307218 7 30.977900797502894 30.822369143093155 19.601244074051344 18.59848598535261 8 28.23983703057379 34.563457474493475 18.49517550656957 18.701529988363166 9 28.276281160535284 15.499448517804836 17.945405277839104 38.27886504382078 10-11 27.31323825472166 24.7084719016118 25.8994442785868 22.078845565079742 12-13 26.94521644012754 21.997287530295885 26.328373606210114 24.729122423366462 14-15 24.506974323883767 23.440450003761676 23.35626014308694 28.69631552926762 16-17 25.21359061988192 26.520616975376786 24.012928519190126 24.252863885551164 18-19 25.18765783715427 25.936296101549654 25.210275983939773 23.665770077356303 20-21 25.697835663202785 24.9457062906811 25.117381667255188 24.239076378860933 22-23 25.892288916507116 24.40352468285692 24.837190554566526 24.866995846069443 24-25 25.308404376114403 24.875866860756595 24.8168651341444 24.998863628984605 26-27 24.927999842338693 25.084630978476547 25.063109313931438 24.92425986525333 28-29 25.022993867630124 25.462402367041477 24.861773655242256 24.652830110086153 30-31 25.59796426264415 25.023493152448562 24.82166586994074 24.556876714966545 32-33 25.121142891177733 25.040713102330557 24.89902053445057 24.939123472041132 34-35 25.119711518866065 25.344757081130613 24.998353980612357 24.53717741939096 36-37 25.27694350719592 25.247214222776215 24.93360161326141 24.542240656766456 38-39 25.27735120226381 25.340488607392587 24.87900343203292 24.503156758310684 40-41 25.223648969427696 25.14948001110743 24.955234375699813 24.671636643765062 42-43 25.156278406622977 25.006515331852047 24.866939459805103 24.970266801719866 44-45 24.937671046409996 25.06069460962015 25.14847903024745 24.8531553137224 46-47 24.93313836267185 25.003688724905754 25.160977298277125 24.90219561414527 48-49 24.913004916865145 25.273681012132176 25.041597095603574 24.771716975399105 50-51 24.87560217325349 25.274606677167966 25.135761170502775 24.714029979075775 52-53 25.097748323730716 25.14084871270893 24.997934539800916 24.76346842375944 54-55 24.761053961984917 24.93674414543305 25.233247917499273 25.06895397508276 56-57 24.81840470193115 25.15349566190876 24.93237055695494 25.09572907920515 58-59 24.82462552486967 25.263820122084326 25.08363772322703 24.827916629818976 60-61 25.19948638092221 25.139526839059677 25.15938647820533 24.50160030181278 62-63 24.904509053392538 25.209766630425634 25.18498152423701 24.70074279194482 64-65 24.795615314606327 24.98175846392721 25.187604039445155 25.03502218202131 66-67 24.890688897287866 25.21058396579427 24.936858396221176 24.961868740696687 68-69 24.758937185412663 25.2268790271825 25.090683322709484 24.923500464695355 70-71 24.682596186384025 25.066340649971526 25.284493834669853 24.9665693289746 72-73 24.841674021553736 25.425273675683734 24.97641286597 24.756639436792526 74-75 24.72001117537549 25.33741037536904 25.07660684079836 24.86597160845711 76-77 24.8240527640216 25.01036564069958 25.03864167569649 25.12693991958233 78-79 24.69400197524252 25.085602425459896 25.186357258277404 25.03403834102018 80-81 24.84702958659368 25.338684219363937 24.959711634259104 24.854574559783277 82-83 24.89669803135723 25.32186217821339 25.00320737079557 24.77823241963381 84-85 24.829746022705905 25.343261569211094 24.859543267121214 24.967449140961786 86-87 24.617182224753595 25.504296765157335 24.960820451097653 24.917700558991417 88-89 24.610813703298202 25.243184062225026 25.066239474114326 25.07976276036244 90-91 24.81132119827523 25.062527244773424 25.12951552383961 24.996636033111734 92-93 24.87213542955041 25.252074588244405 25.078155938474573 24.797634043730614 94-95 24.860791504369622 25.051283216558563 25.127533630439807 24.960391648632005 96-97 24.64398508351846 25.05643381287056 25.19855097804057 25.10103012557041 98-99 24.518167010961005 25.14212774797475 25.056949596920425 25.28275564414382 100-101 24.535047429337308 25.24259781986008 25.17649023575536 25.045864515047256 102-103 24.42214621173795 25.106979284655782 25.350061111832417 25.120813391773854 104-105 24.47038961102919 25.189274084275183 25.23652964642945 25.103806658266176 106-107 24.523116237507097 25.065811033689876 25.273675407446405 25.13739732135662 108-109 24.50318508697815 25.097867489456547 25.26277471291233 25.13617271065297 110-111 24.321491144716546 25.46362673210168 25.24112261163428 24.973759511547495 112-113 24.25288681405638 25.49952488026871 25.26880360486603 24.97878470080888 114-115 24.27040839399178 25.60639417714729 25.162677469892408 24.96051995896852 116-117 24.250488511337114 25.428892694950665 25.19787255523273 25.122746238479493 118-119 24.295486932027867 25.330638955460156 25.25660492225304 25.117269190258934 120-121 24.20759626846043 25.544996166609774 25.315373293861537 24.932034271068257 122-123 24.19165038368444 25.58193986895566 25.198154697322323 25.028255050037572 124-125 24.136628569567907 25.56435467916135 25.212778977318024 25.086237773952725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 557.0 1 465.0 2 328.5 3 255.0 4 210.0 5 181.5 6 172.5 7 194.0 8 224.5 9 229.0 10 219.0 11 237.5 12 272.5 13 308.5 14 330.5 15 363.5 16 426.0 17 478.0 18 504.5 19 522.5 20 594.5 21 686.5 22 789.5 23 959.0 24 1145.0 25 1384.5 26 1798.0 27 2413.0 28 3290.5 29 4731.0 30 6697.0 31 8976.0 32 12128.0 33 15201.5 34 18266.0 35 22123.5 36 26580.0 37 31365.0 38 35996.0 39 41148.5 40 46261.5 41 50968.0 42 55800.0 43 62226.5 44 69137.5 45 75419.5 46 81390.0 47 87038.5 48 94062.5 49 100562.0 50 103989.0 51 105106.0 52 104357.5 53 103654.0 54 103516.5 55 100392.5 56 93811.5 57 85940.5 58 78708.5 59 71402.5 60 62047.5 61 53057.5 62 44765.0 63 35129.0 64 26685.0 65 20853.0 66 16146.5 67 12729.5 68 10295.5 69 8082.0 70 6411.5 71 5016.0 72 3918.0 73 3206.5 74 2529.0 75 1927.5 76 1432.0 77 939.0 78 607.0 79 417.0 80 280.0 81 194.5 82 145.0 83 122.5 84 103.5 85 75.0 86 58.5 87 49.0 88 37.0 89 28.5 90 28.0 91 27.5 92 23.5 93 20.5 94 21.0 95 19.5 96 14.0 97 9.0 98 5.0 99 6.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025428752039224744 2 0.011460845989509744 3 0.010431160607639729 4 0.011505614919156268 5 0.016788348617445912 6 0.09446244155416235 7 0.06039328609315877 8 0.050409814781984266 9 0.02771196745119739 10-11 0.05320787288489192 12-13 0.013564985682896298 14-15 0.02883119069236045 16-17 0.035770374787571425 18-19 0.04349301515159655 20-21 0.031271097358095926 22-23 0.03881466200353495 24-25 0.03162924879526811 26-27 0.04736552756602074 28-29 0.046179150930387894 30-31 0.026615128674857588 32-33 0.030823408061630697 34-35 0.04624630432485768 36-37 0.009132861647890577 38-39 0.02092947460974924 40-41 0.042620021023489364 42-43 0.022294926963968176 44-45 0.017012193265678526 46-47 0.023973761825712765 48-49 0.029704184820467636 50-51 0.02840588586071849 52-53 0.02388422396641972 54-55 0.022362080358437956 56-57 0.02538398310957822 58-59 0.017885187393785713 60-61 0.02325745895136841 62-63 0.02220538910467513 64-65 0.020795167820809674 66-67 0.027577660662257822 68-69 0.02276500072525666 70-71 0.04864144206094663 72-73 0.02162339301927034 74-75 0.009401475225769711 76-77 0.015758663235575898 78-79 0.024555757911117554 80-81 0.01866864366259986 82-83 0.02482437148899669 84-85 0.011818997426681924 86-87 0.017191268984264617 88-89 0.022697847330786877 90-91 0.020839936750456194 92-93 0.007901716082611211 94-95 0.011707075102565618 96-97 0.01448274874065001 98-99 0.006178112291220096 100-101 0.009468628620239497 102-103 0.00367105223101484 104-105 0.004141125992303326 106-107 0.0010744543115165386 108-109 0.014102212838654567 110-111 0.0011416077059863221 112-113 0.0019922173692702486 114-115 0.012759144949258895 116-117 0.005394656022405954 118-119 0.2837454760996592 120-121 0.001320683424572412 122-123 9.1776305775371E-4 124-125 0.31985161785957955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2233692.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.61402473087733 #Duplication Level Percentage of deduplicated Percentage of total 1 79.63759329407112 47.475174561403065 2 10.934583865822512 13.037091059979911 3 3.5738248898210556 6.3915025609685205 4 1.761271995922376 4.199860492908727 5 0.9987793449186908 2.9770628284336147 6 0.637519027338947 2.2803045037313305 7 0.4310128438022913 1.798608723082889 8 0.330461517973441 1.5760112884057578 9 0.24714536970307757 1.3259997163440977 >10 1.3608184827125271 14.264373371193303 >50 0.06820657678995799 2.7310515009881478 >100 0.01833270925877859 1.7445874504547987 >500 4.5008186528560187E-4 0.19837194210595427 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.476892964652244E-5 6 0.0 0.0 0.0 0.0 4.476892964652244E-5 7 0.0 0.0 0.0 0.0 4.476892964652244E-5 8 0.0 0.0 0.0 0.0 8.953785929304487E-5 9 0.0 0.0 0.0 0.0 2.238446482326122E-4 10-11 0.0 0.0 0.0 0.0 2.6861357787913464E-4 12-13 0.0 0.0 0.0 2.238446482326122E-5 3.8053590199544073E-4 14-15 0.0 0.0 0.0 4.476892964652244E-5 4.700737612884856E-4 16-17 0.0 0.0 0.0 8.953785929304487E-5 5.819960854047917E-4 18-19 0.0 0.0 0.0 1.7907571858608975E-4 7.834562688141427E-4 20-21 0.0 0.0 0.0 2.0146018340935098E-4 8.058407336374039E-4 22-23 0.0 0.0 0.0 3.581514371721795E-4 8.282251984606651E-4 24-25 0.0 0.0 0.0 5.14842690935008E-4 8.506096632839264E-4 26-27 0.0 0.0 0.0 9.177630577537101E-4 8.506096632839264E-4 28-29 0.0 0.0 4.476892964652244E-5 0.003089056145610048 8.506096632839264E-4 30-31 0.0 0.0 4.476892964652244E-5 0.010990772228221259 8.729941281071876E-4 32-33 0.0 0.0 4.476892964652244E-5 0.02648082188591802 8.953785929304488E-4 34-35 0.0 0.0 4.476892964652244E-5 0.048372828483067495 8.953785929304488E-4 36-37 0.0 0.0 4.476892964652244E-5 0.07980061709492625 8.953785929304488E-4 38-39 0.0 0.0 4.476892964652244E-5 0.13097150368090138 9.401475225769712E-4 40-41 0.0 0.0 4.476892964652244E-5 0.19337939160815368 9.401475225769712E-4 42-43 0.0 0.0 4.476892964652244E-5 0.25191476712098176 9.401475225769712E-4 44-45 0.0 0.0 4.476892964652244E-5 0.3194710819575841 9.401475225769712E-4 46-47 0.0 0.0 4.476892964652244E-5 0.3917281344070713 9.401475225769712E-4 48-49 0.0 0.0 4.476892964652244E-5 0.46785769927098275 9.401475225769712E-4 50-51 0.0 0.0 6.715339446978366E-5 0.5456213300669922 9.401475225769712E-4 52-53 0.0 0.0 8.953785929304487E-5 0.6282647741944727 9.625319874002324E-4 54-55 0.0 0.0 8.953785929304487E-5 0.7216303769722952 9.849164522234936E-4 56-57 0.0 0.0 8.953785929304487E-5 0.8238602278201292 9.849164522234936E-4 58-59 0.0 0.0 8.953785929304487E-5 0.9296715930396849 9.849164522234936E-4 60-61 0.0 0.0 8.953785929304487E-5 1.0403403871258885 9.849164522234936E-4 62-63 0.0 0.0 8.953785929304487E-5 1.1577692895887166 0.001208761100456106 64-65 0.0 0.0 8.953785929304487E-5 1.2825850654432214 0.001208761100456106 66-67 2.238446482326122E-5 0.0 8.953785929304487E-5 1.4308597604325035 0.001208761100456106 68-69 4.476892964652244E-5 0.0 8.953785929304487E-5 1.5932366682604404 0.001208761100456106 70-71 4.476892964652244E-5 0.0 8.953785929304487E-5 1.7612320767590162 0.001320683424572412 72-73 8.953785929304487E-5 0.0 8.953785929304487E-5 1.9247729767577626 0.0014549902135119791 74-75 8.953785929304487E-5 0.0 8.953785929304487E-5 2.1005134100851865 0.001522143607981763 76-77 8.953785929304487E-5 0.0 8.953785929304487E-5 2.3051745719642636 0.001522143607981763 78-79 8.953785929304487E-5 0.0 8.953785929304487E-5 2.5338542645987 0.001522143607981763 80-81 8.953785929304487E-5 0.0 8.953785929304487E-5 2.7598702059191687 0.001544528072805024 82-83 8.953785929304487E-5 0.0 8.953785929304487E-5 2.9885722830184287 0.0016564503969213303 84-85 8.953785929304487E-5 0.0 8.953785929304487E-5 3.231533264210106 0.0016788348617445916 86-87 8.953785929304487E-5 0.0 8.953785929304487E-5 3.5150548956615326 0.0017236037913911139 88-89 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 3.8367868085662664 0.0017459882562143752 90-91 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 4.167741120978183 0.0017459882562143752 92-93 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 4.49683752280977 0.0018802950451539424 94-95 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 4.837999151181094 0.0018802950451539424 96-97 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 5.216833833849966 0.0019698329044469873 98-99 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 5.642877352831098 0.002059370763740032 100-101 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 6.099095130393985 0.002059370763740032 102-103 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 6.563841389054534 0.0021041396933865544 104-105 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 7.042824167342678 0.0021265241582098158 106-107 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 7.553324272101973 0.002148908623033077 108-109 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 8.114099884854312 0.002148908623033077 110-111 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 8.71192178688915 0.002148908623033077 112-113 8.953785929304487E-5 4.476892964652244E-5 8.953785929304487E-5 9.297947075962128 0.002171293087856338 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12090 0.0 38.60544 1 TACATGG 12555 0.0 37.169777 2 GTATCAA 4250 0.0 37.12066 1 CATGGGG 10930 0.0 36.05031 4 ACATGGG 12965 0.0 35.12045 3 ATGGGGG 6620 0.0 33.983337 5 GGTATCA 2810 0.0 33.68601 1 TCAACGC 4865 0.0 32.054543 4 CAACGCA 4980 0.0 31.192009 5 CGCCTTA 905 0.0 30.912907 2 ATCAACG 5235 0.0 29.67529 3 AACGCAG 5225 0.0 29.496979 6 TATCAAC 5335 0.0 29.008785 2 ACGCAGA 6210 0.0 24.524796 7 GAGTACA 8520 0.0 23.687479 1 AGTACAT 8415 0.0 22.847517 2 ATGGGGA 3900 0.0 22.738085 5 CGCAGAG 6725 0.0 22.644657 8 TGGGGGG 5355 0.0 21.669016 6 TATACCG 220 4.170739E-6 21.642105 5 >>END_MODULE