FastQCFastQC Report
Mon 15 Aug 2016
SRR2921582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2233692
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22700.10162547029760594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52950.042.590261
GGTATCA35000.040.2281
GTACATG126350.034.7997931
TCAACGC65500.033.9652634
CATGGGG107400.033.4317934
TACATGG129950.033.324742
CAACGCA67200.032.9802555
ACATGGG130400.032.935283
ATCAACG69500.032.01333
TATCAAC70400.031.8582292
AACGCAG70100.031.7136656
ATGGGGG63750.030.3727055
ACGCAGA83500.026.337597
CGCAGAG90050.024.4185778
ATGGGGA38850.023.9229035
GCAGAGT100550.021.8093879
GAGTACA92750.021.419771
GTATAGG5600.021.3072031
AGTACAT91900.021.0947482
TGGGGGG42750.020.9127316
Produced by FastQC (version 0.11.5)