##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921582_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2233692
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.846350347317355	33.0	31.0	34.0	30.0	34.0
2	32.013347408684815	34.0	31.0	34.0	30.0	34.0
3	32.07884927733994	34.0	31.0	34.0	30.0	34.0
4	35.47706532503138	37.0	35.0	37.0	35.0	37.0
5	35.336143479047244	37.0	35.0	37.0	33.0	37.0
6	35.36252222777357	37.0	36.0	37.0	33.0	37.0
7	35.25666967513874	37.0	36.0	37.0	33.0	37.0
8	35.29449404841849	37.0	36.0	37.0	33.0	37.0
9	37.037083447494105	39.0	38.0	39.0	34.0	39.0
10-11	36.9858785365216	39.0	38.0	39.0	34.0	39.0
12-13	36.92087225991766	39.0	38.0	39.0	34.0	39.0
14-15	38.37118814948525	41.0	39.0	41.0	34.0	41.0
16-17	38.373384513173704	41.0	39.0	41.0	34.0	41.0
18-19	38.374613420292505	41.0	39.0	41.0	34.0	41.0
20-21	38.304015952065015	41.0	39.0	41.0	34.0	41.0
22-23	38.31017570909508	41.0	39.0	41.0	34.0	41.0
24-25	38.270178923504226	41.0	39.0	41.0	34.0	41.0
26-27	38.20102256712205	41.0	39.0	41.0	34.0	41.0
28-29	38.13198395302486	41.0	39.0	41.0	34.0	41.0
30-31	38.03455579372626	41.0	38.5	41.0	33.5	41.0
32-33	37.929380371152334	41.0	38.0	41.0	33.0	41.0
34-35	37.803842696307285	41.0	38.0	41.0	33.0	41.0
36-37	37.732535640544896	41.0	38.0	41.0	33.0	41.0
38-39	37.65505897858792	40.5	38.0	41.0	33.0	41.0
40-41	37.543627769629836	40.0	38.0	41.0	33.0	41.0
42-43	37.39395516481234	40.0	38.0	41.0	32.5	41.0
44-45	37.253203216916205	40.0	37.5	41.0	32.0	41.0
46-47	37.08579629599784	40.0	37.0	41.0	31.5	41.0
48-49	36.91872670896436	40.0	37.0	41.0	31.0	41.0
50-51	36.67895170865097	40.0	36.0	41.0	31.0	41.0
52-53	36.542538989260834	40.0	36.0	41.0	31.0	41.0
54-55	36.40507822922766	40.0	35.0	41.0	31.0	41.0
56-57	36.200968172872535	39.0	35.0	41.0	30.0	41.0
58-59	36.000964322744586	39.0	35.0	41.0	30.0	41.0
60-61	35.737287638582224	38.5	35.0	41.0	30.0	41.0
62-63	35.377479527168475	37.5	35.0	40.5	29.5	41.0
64-65	35.0854457552787	37.0	35.0	40.0	29.0	41.0
66-67	34.776077229985155	36.5	35.0	39.5	29.0	41.0
68-69	34.40595211873436	36.0	35.0	39.0	29.0	41.0
70-71	34.031453083057116	35.5	35.0	39.0	29.0	40.5
72-73	33.713859610008896	35.0	34.5	37.0	29.0	39.5
74-75	33.39892944058536	35.0	34.0	37.0	28.0	39.0
76-77	33.08272067053112	35.0	34.0	36.5	28.0	39.0
78-79	32.83187946234307	35.0	34.0	36.0	28.0	37.5
80-81	32.59074371041307	35.0	34.0	36.0	27.0	37.0
82-83	32.379900183194465	35.0	34.0	35.0	27.0	37.0
84-85	32.17916883795975	35.0	34.0	35.0	26.5	36.0
86-87	32.062274700361556	35.0	34.0	35.0	26.0	36.0
88-89	31.974637953665948	35.0	34.0	35.0	26.0	36.0
90-91	31.878504064123433	35.0	34.0	35.0	26.0	35.5
92-93	31.80444819608075	35.0	34.0	35.0	26.0	35.0
94-95	31.74264043565541	35.0	34.0	35.0	25.0	35.0
96-97	31.68090945394441	35.0	33.5	35.0	25.0	35.0
98-99	31.61064394733025	35.0	33.0	35.0	25.0	35.0
100-101	31.543444888552227	35.0	33.0	35.0	25.0	35.0
102-103	30.845501752255906	34.0	31.5	35.0	23.5	35.0
104-105	31.370683155958833	35.0	33.0	35.0	24.5	35.0
106-107	31.471688800425483	35.0	33.0	35.0	25.0	35.0
108-109	31.45713531677599	35.0	33.0	35.0	24.5	35.0
110-111	31.392164407626478	35.0	33.0	35.0	24.0	35.0
112-113	31.353607614657705	35.0	33.0	35.0	24.0	35.0
114-115	31.22663375254959	35.0	33.0	35.0	23.5	35.0
116-117	31.419949572277645	35.0	33.5	35.0	24.5	35.0
118-119	31.4054028039676	35.0	34.0	35.0	24.5	35.0
120-121	31.325540405749763	35.0	34.0	35.0	24.0	35.0
122-123	31.189623054566162	35.0	34.0	35.0	23.0	35.0
124-125	30.55057254984125	34.5	32.5	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33031.0
3	8750.0
4	4930.0
5	3908.0
6	3982.0
7	4440.0
8	4801.0
9	5267.0
10	5414.0
11	5804.0
12	6337.0
13	7354.0
14	9000.0
15	8290.0
16	5638.0
17	5052.0
18	4603.0
19	4256.0
20	4310.0
21	4544.0
22	4967.0
23	6254.0
24	7943.0
25	10385.0
26	12518.0
27	15214.0
28	18356.0
29	22533.0
30	27168.0
31	34382.0
32	45237.0
33	62089.0
34	97323.0
35	176520.0
36	414350.0
37	850400.0
38	288044.0
39	298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.57388657305099	17.195398654739243	10.651615436869424	27.579099335340345
2	16.214709778627608	19.133868807968277	37.12387354011829	27.52754787328583
3	19.261519638905845	22.795927077180338	28.037193163787943	29.905360120125874
4	13.686540156198499	14.308777925022031	33.41957212464487	38.585109794134596
5	15.014602455863507	37.10555233643772	32.31141517097098	15.568430036727795
6	35.787857568780936	32.74976334189469	16.274595978805827	15.187783110518552
7	31.43313006327441	31.02524615923508	18.712984289347627	18.82863948814288
8	28.8917444736373	34.02651184997998	18.134675109801663	18.94706856658106
9	28.481257840116047	14.965827058628056	17.92188389395528	38.63103120730062
10-11	27.578632179405076	24.435719295086177	25.70896691648249	22.27668160902625
12-13	27.214306447971502	21.606062017781824	26.427795835140067	24.751835699106607
14-15	24.5832594193316	23.109906984828367	23.562663842703838	28.744169753136195
16-17	25.05303956517523	26.321823454674657	24.22957973941241	24.395557240737702
18-19	25.005715800757372	25.583834618203543	25.7929176022255	23.61753197881358
20-21	25.600247722881747	24.50728021815018	25.73557284013254	24.156899218835537
22-23	25.482050990471578	24.04210987212313	25.538170326101024	24.937668811304274
24-25	24.95784816564529	24.49811782993492	25.53931067088349	25.004723333536294
26-27	24.70955194666232	24.757523444026685	25.713304602667066	24.81962000664393
28-29	24.801709000683207	25.14886375541394	25.491787645517118	24.557639598385737
30-31	25.31348500955503	24.69539063935787	25.45925607064166	24.531868280445433
32-33	24.95230001994635	24.667432134843583	25.46331606813816	24.91695177707191
34-35	24.931388727962688	24.886729841269556	25.65804142016603	24.523840010601727
36-37	24.99440349293676	24.810793289205996	25.664193458265277	24.530609759591965
38-39	25.028395316529988	24.98418502867289	25.55343445662534	24.433985198171776
40-41	25.0539427874033	24.744511871218716	25.53797843094372	24.663566910434266
42-43	24.94813892209993	24.666800829060918	25.362826077856422	25.02223417098273
44-45	24.770615708895285	24.768556242243424	25.560846494519136	24.899981554342162
46-47	24.83927205912639	24.73714937580252	25.426085741864657	24.99749282320643
48-49	24.75337463193668	25.000660353833254	25.428569637781372	24.817395376448694
50-51	24.802795591718432	25.047662815214228	25.467332867878923	24.682208725188417
52-53	24.983653616496046	24.943137417739244	25.233667662857773	24.839541302906937
54-55	24.625287319269802	24.701868481870793	25.521965205602847	25.15087899325656
56-57	24.727864576629603	24.904255383445186	25.231876684022954	25.136003355902254
58-59	24.752885041122777	24.935879269921298	25.446830495187978	24.864405193767944
60-61	25.05361084125371	24.863656102682107	25.539063164539392	24.543669891524786
62-63	24.776069555375933	24.932336798551802	25.51230070106486	24.779292945007406
64-65	24.613487445896748	24.668978534193613	25.550769756976344	25.166764262933295
66-67	24.707071670316587	24.948004563913255	25.296185389612642	25.048738376157516
68-69	24.569683465660074	24.962600879133966	25.404921171405604	25.062794483800353
70-71	24.525068809844868	24.792697471271776	25.570893390852454	25.111340328030902
72-73	24.66976593842436	25.129038078153044	25.270645309031032	24.930550674391565
74-75	24.5049271675519	25.087130087475945	25.349443753104595	25.058498991867562
76-77	24.5176408016092	24.717917746547478	25.343371878493432	25.421069573349893
78-79	24.396866438628305	24.860404662512252	25.49010302913768	25.25262586972176
80-81	24.59405773043016	25.14679732031095	25.175337512960606	25.08380743629829
82-83	24.612541623527438	25.110177127347004	25.20690600777905	25.070375241346504
84-85	24.539303397502437	25.107430398286574	25.06460821733701	25.28865798687398
86-87	24.28792778950724	25.23696194461904	25.26301746167332	25.2120928042004
88-89	24.210216699762476	24.910656984827412	25.355575686880737	25.523550628529375
90-91	24.441089736788264	24.821023809619742	25.40669488023559	25.3311915733564
92-93	24.368153711433806	24.990643293703876	25.372858030561062	25.268344964301253
94-95	24.353214486061432	24.78791183650956	25.38297644678623	25.475897230642776
96-97	24.144572358370006	24.81900250418203	25.438590011431895	25.597835126016072
98-99	24.074709134180605	24.868893250408743	25.290350973014885	25.76604664239576
100-101	24.005749225945205	24.963714782521492	25.44717445377429	25.583361537759007
102-103	23.90421462885698	24.80502296566555	25.571142078216454	25.719620327261012
104-105	24.04682471889589	24.865961824638312	25.39766001758523	25.689553438880562
106-107	24.059533200398626	24.794128786691726	25.394707742118605	25.751630270791043
108-109	23.990240135205465	24.785982506113907	25.42946203222322	25.794315326457408
110-111	23.82646566299136	25.156058997430485	25.386574370979325	25.63090096859883
112-113	23.758186569392606	25.1287220378486	25.391292162874958	25.72179922988384
114-115	23.718847540305468	25.246184344126227	25.25892110461066	25.776047010957644
116-117	23.56280723118111	25.07881682977714	25.39594208608293	25.96243385295882
118-119	23.692640229436844	24.966836687868206	25.375630752094224	25.964892330600726
120-121	23.527061339603524	25.099831508097665	25.482615024767885	25.890492127530933
122-123	23.371072538695408	25.251011455174748	25.33038779798027	26.047528208149572
124-125	23.25039477921159	25.20736132420835	25.29609841391415	26.246145482665906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	14.0
1	15.5
2	26.0
3	54.0
4	55.5
5	60.0
6	172.5
7	406.0
8	677.5
9	768.5
10	647.5
11	520.0
12	478.5
13	510.5
14	568.5
15	592.5
16	616.0
17	640.5
18	681.5
19	750.5
20	795.5
21	847.0
22	894.0
23	1023.5
24	1147.0
25	1295.0
26	1713.5
27	2333.0
28	3209.5
29	4570.0
30	6366.0
31	8580.0
32	11804.5
33	14855.5
34	17861.0
35	21626.5
36	25834.0
37	30534.5
38	35267.0
39	40524.0
40	45678.0
41	50528.0
42	55153.5
43	61084.5
44	68239.5
45	75132.5
46	81607.0
47	87478.5
48	94442.5
49	100903.5
50	105311.5
51	107081.0
52	105829.5
53	105074.0
54	104768.0
55	101679.0
56	95355.0
57	87071.0
58	79544.0
59	72000.5
60	62388.0
61	53210.0
62	44733.0
63	34990.0
64	26136.5
65	19971.5
66	15407.5
67	12079.5
68	9723.5
69	7708.0
70	6070.0
71	4661.0
72	3521.5
73	2929.5
74	2316.0
75	1687.5
76	1160.5
77	710.0
78	421.0
79	228.0
80	141.5
81	81.5
82	42.5
83	28.0
84	16.0
85	8.5
86	7.0
87	6.5
88	5.0
89	4.5
90	3.5
91	1.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023235074486545146
2	0.0015669125376282854
3	0.03138301968221223
4	0.1717783830537066
5	0.022653078401140354
6	0.0702424506153937
7	0.0534541019979478
8	0.04611199753591811
9	0.03514360977252012
10-11	0.06480302566334123
12-13	0.058199608540479175
14-15	0.06124389575644269
16-17	0.051954342854789295
18-19	0.03789689894578124
20-21	0.024690064700057128
22-23	0.002641366849144824
24-25	0.004476892964652244
26-27	0.00331290079384266
28-29	0.004745506542531378
30-31	0.002350368806442428
32-33	0.008976170394127748
34-35	0.00420827938677311
36-37	0.006983953024857501
38-39	0.0021936775526795993
40-41	0.003380054188312444
42-43	0.0036038988365450562
44-45	0.004655968683238334
46-47	0.004655968683238334
48-49	0.00183552611550742
50-51	0.0014549902135119793
52-53	7.834562688141427E-4
54-55	0.005103657979703558
56-57	6.939184095210978E-4
58-59	6.715339446978365E-4
60-61	8.953785929304487E-5
62-63	8.28225198460665E-4
64-65	0.0
66-67	0.0036934366958381016
68-69	7.386873391676202E-4
70-71	0.0
72-73	0.0021936775526795993
74-75	0.004767891007354639
76-77	0.0015669125376282854
78-79	1.5669125376282855E-4
80-81	0.0
82-83	0.005797576389224656
84-85	0.0016340659320980692
86-87	0.0
88-89	0.0011416077059863221
90-91	6.715339446978365E-4
92-93	0.0
94-95	0.003089056145610048
96-97	0.0012759144949258895
98-99	7.16302874344359E-4
100-101	0.0
102-103	0.0014102212838654569
104-105	0.0
106-107	6.267650150513142E-4
108-109	0.002439906665735473
110-111	6.715339446978366E-5
112-113	1.3430678893956732E-4
114-115	0.0
116-117	0.001432605748688718
118-119	0.0021936775526795993
120-121	0.0023951377360889505
122-123	0.0012982989597491506
124-125	0.10661720595319318
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2233692.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.14462699010689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.94300976092546	43.19936582523314
2	12.17826806697739	13.674886360119515
3	4.204805496330774	7.082317084723279
4	1.9781238802496972	4.4424410958736855
5	1.1351738710650967	3.1866956779932774
6	0.7497423145406548	2.5256401553150676
7	0.5111912066827138	2.009044773387652
8	0.3663121150879305	1.6453165650855175
9	0.2802495075919495	1.4161053661120022
>10	1.5535316803741988	15.49646173898665
>50	0.07808736192873507	2.9804292915447514
>100	0.020786166504144256	1.9068366327482247
>500	4.7904782757467277E-4	0.19903011576304516
>1k	2.3952391378732443E-4	0.23542931711429607
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2270	0.10162547029760594	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.238446482326122E-5	1.7907571858608978E-4
14-15	0.0	0.0	0.0	4.476892964652244E-5	2.9099804270239587E-4
16-17	0.0	0.0	0.0	8.953785929304487E-5	3.133825075256571E-4
18-19	0.0	0.0	0.0	1.7907571858608975E-4	3.133825075256571E-4
20-21	0.0	0.0	0.0	2.0146018340935098E-4	4.4768929646522436E-4
22-23	0.0	0.0	0.0	3.581514371721795E-4	5.372271557582693E-4
24-25	0.0	0.0	0.0	5.14842690935008E-4	5.372271557582693E-4
26-27	0.0	0.0	0.0	9.177630577537101E-4	5.819960854047917E-4
28-29	0.0	0.0	0.0	0.003066671680786787	5.819960854047917E-4
30-31	0.0	0.0	0.0	0.010946003298574737	5.819960854047917E-4
32-33	0.0	0.0	0.0	0.026413668491448238	6.267650150513142E-4
34-35	0.0	0.0	0.0	0.04855190420165358	6.715339446978365E-4
36-37	0.0	0.0	0.0	0.08002446174315886	6.715339446978365E-4
38-39	0.0	0.0	0.0	0.1309043502864316	6.715339446978365E-4
40-41	0.0	0.0	0.0	0.19268547319863258	6.715339446978365E-4
42-43	0.0	0.0	0.0	0.2511313108521676	6.715339446978365E-4
44-45	0.0	0.0	0.0	0.31850854997018385	6.939184095210978E-4
46-47	0.0	0.0	0.0	0.39110136939202	7.16302874344359E-4
48-49	0.0	0.0	0.0	0.4678353148061595	7.16302874344359E-4
50-51	0.0	0.0	0.0	0.545778021320755	7.16302874344359E-4
52-53	0.0	0.0	0.0	0.6291377683225798	7.610718039908815E-4
54-55	0.0	0.0	0.0	0.7233763652285096	8.506096632839264E-4
56-57	0.0	0.0	0.0	0.8264792102044507	9.401475225769712E-4
58-59	0.0	0.0	0.0	0.9336336433134023	0.001007300917046755
60-61	0.0	0.0	0.0	1.044414359723722	0.001029685381870016
62-63	0.0	0.0	0.0	1.1622014136237224	0.0010744543115165386
64-65	0.0	0.0	0.0	1.2872634185912828	0.0010744543115165386
66-67	0.0	0.0	0.0	1.4360081873418538	0.0010744543115165386
68-69	0.0	0.0	0.0	1.5994147805516605	0.0010744543115165386
70-71	0.0	0.0	0.0	1.7683951055024596	0.0010744543115165386
72-73	0.0	0.0	0.0	1.9340625296594158	0.0011639921708095835
74-75	0.0	0.0	0.0	2.111213184270705	0.001208761100456106
76-77	0.0	0.0	0.0	2.317172645109532	0.0012535300301026284
78-79	0.0	0.0	0.0	2.5484489356634663	0.0012535300301026284
80-81	0.0	0.0	0.0	2.776501863282852	0.0012535300301026284
82-83	0.0	0.0	0.0	3.008651147964894	0.0012982989597491506
84-85	0.0	0.0	0.0	3.255641332824758	0.001320683424572412
86-87	0.0	0.0	0.0	3.543460781522251	0.001432605748688718
88-89	0.0	0.0	0.0	3.8704754281252742	0.0015669125376282854
90-91	0.0	0.0	0.0	4.206667705305835	0.0015669125376282854
92-93	0.0	0.0	0.0	4.540017155453841	0.0016116814672748078
94-95	0.0	0.0	0.0	4.8853422942822915	0.0016564503969213303
96-97	0.0	0.0	0.0	5.26984024655145	0.0018802950451539424
98-99	0.0	0.0	0.0	5.70116649923087	0.002059370763740032
100-101	0.0	0.0	0.0	6.161659709575	0.002059370763740032
102-103	0.0	0.0	0.0	6.629562177775629	0.0022160620175028607
104-105	0.0	0.0	0.0	7.111656396674206	0.0022832154119726443
106-107	0.0	0.0	0.0	7.627976462287549	0.0022832154119726443
108-109	0.0	0.0	0.0	8.196541868798384	0.0022832154119726443
110-111	0.0	0.0	0.0	8.801459646182195	0.0023055998767959056
112-113	0.0	0.0	0.0	9.398587629807512	0.002372753271265689
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5295	0.0	42.59026	1
GGTATCA	3500	0.0	40.228	1
GTACATG	12635	0.0	34.799793	1
TCAACGC	6550	0.0	33.965263	4
CATGGGG	10740	0.0	33.431793	4
TACATGG	12995	0.0	33.32474	2
CAACGCA	6720	0.0	32.980255	5
ACATGGG	13040	0.0	32.93528	3
ATCAACG	6950	0.0	32.0133	3
TATCAAC	7040	0.0	31.858229	2
AACGCAG	7010	0.0	31.713665	6
ATGGGGG	6375	0.0	30.372705	5
ACGCAGA	8350	0.0	26.33759	7
CGCAGAG	9005	0.0	24.418577	8
ATGGGGA	3885	0.0	23.922903	5
GCAGAGT	10055	0.0	21.809387	9
GAGTACA	9275	0.0	21.41977	1
GTATAGG	560	0.0	21.307203	1
AGTACAT	9190	0.0	21.094748	2
TGGGGGG	4275	0.0	20.912731	6
>>END_MODULE