FastQCFastQC Report
Mon 15 Aug 2016
SRR2921581_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921581_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445572
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29050.043.2357371
GGTATCA17950.037.4731751
TCAACGC35400.033.9620934
GTACATG80200.033.9194151
CAACGCA36550.033.0552145
ATCAACG37750.032.005563
TATACCG2056.91216E-1131.9352385
TACATGG84100.031.9173532
CATGGGG66300.031.6889274
ACATGGG83600.031.5387023
AACGCAG38500.031.5312066
TATCAAC40250.030.609122
ATGGGGG43100.026.5127815
ACGCAGA47550.025.5238157
CGCAGAG50050.024.0111648
CTCTACG4800.023.5591013
TGGGGGG32050.021.909136
TCTACGT3557.8216544E-1121.7952254
ATTAACG1653.2093606E-421.642812
GAGTACA59450.020.9267081
Produced by FastQC (version 0.11.5)