##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921581_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1445572 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.511477117708424 31.0 30.0 33.0 26.0 34.0 2 29.913924038373736 31.0 31.0 34.0 26.0 34.0 3 29.98914132260448 31.0 31.0 34.0 26.0 34.0 4 33.45629411748429 37.0 35.0 37.0 28.0 37.0 5 33.468940322585105 37.0 35.0 37.0 28.0 37.0 6 33.43338899757328 37.0 35.0 37.0 28.0 37.0 7 33.36947865619976 37.0 35.0 37.0 27.0 37.0 8 33.24776559036838 37.0 35.0 37.0 27.0 37.0 9 35.03788949979662 39.0 37.0 39.0 27.0 39.0 10-11 35.06668052507935 39.0 37.0 39.0 27.5 39.0 12-13 35.009898157961004 39.0 37.0 39.0 27.0 39.0 14-15 36.28843011624464 40.0 38.0 41.0 27.0 41.0 16-17 36.26342963200726 40.0 38.0 41.0 27.0 41.0 18-19 36.22008346868921 40.0 38.0 41.0 27.0 41.0 20-21 36.27568014599065 40.0 38.0 41.0 27.0 41.0 22-23 36.21333389135927 40.0 38.0 41.0 27.0 41.0 24-25 36.214019779021726 40.0 37.5 41.0 27.0 41.0 26-27 36.19050590354544 40.0 37.0 41.0 27.0 41.0 28-29 36.14060662492079 40.0 37.0 41.0 27.0 41.0 30-31 36.04580228449361 40.0 37.0 41.0 27.0 41.0 32-33 35.9546878329132 40.0 37.0 41.0 27.0 41.0 34-35 35.81993667558586 40.0 37.0 41.0 25.5 41.0 36-37 35.71315818236657 40.0 36.5 41.0 25.0 41.0 38-39 35.59651473603529 40.0 36.0 41.0 25.0 41.0 40-41 35.49044357527677 40.0 36.0 41.0 24.5 41.0 42-43 35.230964628534586 40.0 35.5 41.0 23.5 41.0 44-45 34.86914660770961 40.0 35.0 41.0 22.5 41.0 46-47 34.60507155644963 39.0 35.0 41.0 21.5 41.0 48-49 34.40677704050715 39.0 34.5 41.0 19.5 41.0 50-51 34.20906637649318 39.0 34.0 40.5 18.5 41.0 52-53 34.0145589427576 38.5 34.0 40.0 17.5 41.0 54-55 33.780022371767025 38.0 34.0 40.0 14.5 41.0 56-57 33.5387133951128 38.0 33.0 40.0 13.5 41.0 58-59 33.563193670048946 38.0 34.0 40.0 13.0 41.0 60-61 33.3583982672603 37.5 33.5 40.0 11.5 41.0 62-63 33.0582599828995 37.0 33.0 40.0 8.0 41.0 64-65 32.77343328454065 36.5 33.0 39.5 7.5 41.0 66-67 32.461518347062615 36.0 33.0 39.0 6.0 41.0 68-69 32.13582927726879 35.5 32.5 39.0 2.0 40.5 70-71 31.777297844728594 35.0 32.0 38.5 2.0 40.0 72-73 31.4397311237351 35.0 32.0 37.0 2.0 39.5 74-75 31.0704759084985 35.0 31.5 37.0 2.0 39.0 76-77 30.768320775443907 35.0 31.0 36.0 2.0 39.0 78-79 30.49456547304458 35.0 31.0 36.0 2.0 38.0 80-81 30.23083180913853 35.0 31.0 35.5 2.0 37.0 82-83 29.992139789647283 35.0 31.0 35.0 2.0 37.0 84-85 29.76665638238704 35.0 31.0 35.0 2.0 36.0 86-87 29.59209088167175 35.0 31.0 35.0 2.0 36.0 88-89 29.42626344450501 34.0 30.5 35.0 2.0 36.0 90-91 29.24055460399067 34.0 30.0 35.0 2.0 35.0 92-93 29.16475692666986 34.0 30.0 35.0 2.0 35.0 94-95 29.039542132802794 34.0 30.0 35.0 2.0 35.0 96-97 28.95367958150822 34.0 30.0 35.0 2.0 35.0 98-99 28.869925192242242 34.0 30.0 35.0 2.0 35.0 100-101 28.767703026898694 34.0 29.5 35.0 2.0 35.0 102-103 27.90219580899464 33.5 28.0 34.5 2.0 35.0 104-105 28.823295207710167 34.0 29.5 35.0 2.0 35.0 106-107 29.10109112517398 34.5 31.0 35.0 2.0 35.0 108-109 29.1117740935768 35.0 31.0 35.0 2.0 35.0 110-111 29.058759785053944 35.0 31.0 35.0 2.0 35.0 112-113 28.99285092683035 35.0 31.0 35.0 2.0 35.0 114-115 28.893074852030892 34.5 31.0 35.0 2.0 35.0 116-117 28.78981226808488 34.0 30.0 35.0 2.0 35.0 118-119 28.52136870387639 34.0 30.0 35.0 2.0 35.0 120-121 28.41929561446957 34.0 30.0 35.0 2.0 35.0 122-123 28.1884887089678 34.0 30.0 35.0 2.0 35.0 124-125 27.415596386759013 33.5 27.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66400.0 3 11290.0 4 5631.0 5 4622.0 6 4922.0 7 5396.0 8 5480.0 9 5639.0 10 6275.0 11 6575.0 12 6817.0 13 6559.0 14 6865.0 15 5987.0 16 5267.0 17 4893.0 18 4399.0 19 4147.0 20 3951.0 21 4037.0 22 4652.0 23 6220.0 24 8813.0 25 11582.0 26 12740.0 27 13900.0 28 16423.0 29 19639.0 30 24307.0 31 30520.0 32 39957.0 33 55160.0 34 85536.0 35 140797.0 36 272558.0 37 409506.0 38 118019.0 39 91.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93118818834819 18.463317273461357 13.05354144048956 24.5519530977009 2 16.17244258843764 19.47990759632129 39.349811851000105 24.997837964240965 3 19.635093661876336 24.289545126233904 28.017340384610062 28.058020827279694 4 13.27586326181861 15.552203903444745 36.002808930461676 35.16912390427497 5 14.499275575906776 37.680984940004954 32.59271867298237 15.227020811105898 6 33.143459784131366 34.477283381771166 17.988129787088184 14.391127047009283 7 29.179042644429476 32.67306583454872 19.89330413167879 18.254587389343012 8 26.924272175269014 34.84048867020713 19.090557363932785 19.14468179059107 9 27.37264057593543 16.578003019730662 18.61299978825903 37.43635661607488 10-11 25.986264932317898 26.060498252661844 26.654364815413402 21.29887199960686 12-13 26.024643542459934 23.50580492102879 26.797249282795356 23.67230225371592 14-15 23.769468303189143 24.785565387376636 24.186660438885575 27.25830587054865 16-17 24.18946543818346 27.57253462548286 24.719037752744917 23.51896218358876 18-19 24.20254704644555 26.953167578880198 25.949796131780918 22.894489242893336 20-21 24.994144038319615 25.873628173939885 25.82006818173168 23.312159606008823 22-23 24.93609797468556 25.391604275613837 25.665205637011585 24.007092112689023 24-25 24.441979384610807 25.72224965235621 25.72304545301991 24.112725510013075 26-27 24.091505398558287 25.952252666577387 25.835010812342784 24.121231122521543 28-29 24.23107801339904 26.23761142793865 25.71147776978019 23.819832788882124 30-31 24.803165270593478 25.806912543000564 25.723532185682867 23.66639000072309 32-33 24.277061453495897 25.919671111314095 25.698476723901102 24.104790711288906 34-35 24.37041266545549 26.201020849554457 25.728730859070854 23.699835625919196 36-37 24.444301465374043 26.056690278968308 25.70022270143272 23.798785554224928 38-39 24.47730243287807 26.23928986336554 25.581511206073998 23.701896497682394 40-41 24.462258431538647 25.94561543380588 25.715639213471636 23.876486921183833 42-43 24.308468864925082 25.92415766294063 25.601926330319984 24.165447141814305 44-45 24.083217211895448 25.969296892189032 25.90560883842123 24.041877057494297 46-47 24.130616791907116 25.896541702993314 25.768118348763508 24.20472315633606 48-49 24.102941853187247 26.141794507262862 25.687301423093412 24.067962216456486 50-51 24.153474191463538 26.131999485171697 25.77909298878588 23.935433334578885 52-53 24.24629496109173 26.003706076831207 25.703538763653878 24.046460198423187 54-55 24.042947735505052 25.841016575044367 25.907475895090453 24.208559794360127 56-57 24.20863839512759 26.01755882753863 25.539389385129663 24.234413392204118 58-59 24.119425228982653 26.09281356335613 25.76620452693063 24.021556680730587 60-61 24.40722262066503 26.04099959210066 25.786953379423537 23.76482440781077 62-63 24.290109062829103 26.112839411522938 25.707330776863348 23.889720748784615 64-65 24.074845441430345 25.792097533636166 25.825067722081723 24.307989302851766 66-67 24.06160508268919 25.972976329983137 25.717845427486118 24.24757315984155 68-69 24.069324126096703 25.984784534056626 25.761119260468853 24.18477207937782 70-71 24.05497746646443 25.84115336819517 25.952099409868108 24.151769755472294 72-73 24.143443799797264 26.130041135178704 25.604244292450705 24.122270772573316 74-75 24.098489382089127 26.07405383154552 25.65784694572914 24.169609840636213 76-77 24.01900888658875 25.765537692586577 25.78795481151631 24.427498609308362 78-79 23.980862058466972 25.904343505914785 25.83175799478547 24.283036440832774 80-81 24.14503140206755 26.104815168198424 25.58208162115898 24.168071808575046 82-83 24.198710685437486 26.063348917250472 25.672984075065745 24.064956322246296 84-85 24.14903648908842 26.208306264533267 25.45141764408385 24.191239602294466 86-87 23.895312170809444 26.273735017451315 25.62072473900865 24.210228072730594 88-89 23.95916329664802 25.96716277354409 25.701493786929948 24.37218014287795 90-91 24.176736977211636 25.889479711192838 25.746667543097928 24.1871157684976 92-93 24.166225626018285 25.969857033647077 25.737333485535597 24.12658385479904 94-95 24.06504093164683 25.908858981698806 25.693381924349527 24.332718162304833 96-97 23.874530690497714 25.877690497366885 25.82918984890422 24.41858896323118 98-99 23.82806946541517 25.90843446843322 25.660452414184338 24.603043651967273 100-101 23.802880482628723 26.100832458441936 25.753389591588586 24.34289746734075 102-103 23.682174283153657 25.949739037734286 25.9050145086649 24.463072170447152 104-105 23.758437780502696 26.006264235917143 25.7612637776006 24.47403420597956 106-107 23.756932448876107 25.89050449065654 25.861968777433198 24.490594283034152 108-109 23.721425163948233 25.94375962353742 25.775703385236582 24.559111827277764 110-111 23.648222597550205 26.218523669702304 25.81061678578507 24.32263694696242 112-113 23.593066106229035 26.321256391223468 25.745760917914932 24.33991658463256 114-115 23.586746932102553 26.350397233651428 25.704300141624536 24.35855569262148 116-117 23.51838963727129 26.245012065129565 25.76569297012497 24.47090532747418 118-119 23.471578611465674 26.071265053554583 25.879800488373384 24.57735584660636 120-121 23.421423187913533 26.144883904090516 25.925590285500228 24.50810262249572 122-123 23.345401366741967 26.26286314352036 25.842194119972646 24.549541369765027 124-125 23.308587915052488 26.17109904008133 25.904341233477275 24.615971811388906 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 483.0 1 375.0 2 247.0 3 193.5 4 155.0 5 147.5 6 150.0 7 174.5 8 218.0 9 259.5 10 259.5 11 253.0 12 261.0 13 279.5 14 332.5 15 395.0 16 431.0 17 460.0 18 507.5 19 585.5 20 685.5 21 788.0 22 921.5 23 1141.5 24 1408.5 25 1821.0 26 2458.0 27 3442.5 28 4734.0 29 6251.5 30 8214.5 31 10658.5 32 13598.0 33 16470.0 34 19296.0 35 22292.0 36 25510.0 37 28680.5 38 30984.5 39 33276.0 40 35853.0 41 38263.5 42 40760.0 43 43848.0 44 46959.0 45 49863.0 46 52887.5 47 55594.5 48 58554.0 49 61291.5 50 62617.5 51 62540.5 52 61417.0 53 60287.5 54 59324.5 55 57568.0 56 54192.5 57 49673.5 58 45348.0 59 40417.0 60 34305.5 61 29035.0 62 24449.0 63 19098.5 64 14316.5 65 10992.5 66 8365.5 67 6421.5 68 5032.0 69 3842.5 70 3080.0 71 2447.0 72 1933.5 73 1653.5 74 1312.5 75 954.0 76 715.5 77 479.0 78 314.0 79 235.5 80 165.0 81 114.5 82 92.5 83 67.5 84 46.5 85 45.0 86 37.0 87 27.0 88 25.5 89 22.0 90 20.5 91 17.0 92 16.0 93 18.0 94 16.5 95 13.5 96 8.5 97 6.5 98 4.5 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02455775291718434 2 0.012105934536640169 3 0.011068283004928153 4 0.012590171918105774 5 0.019784555871309072 6 0.10051384503850379 7 0.06260497574662487 8 0.05209010689194312 9 0.02836247520012839 10-11 0.05554894533098317 12-13 0.014077472446892995 14-15 0.030057306035258014 16-17 0.037217101604070914 18-19 0.04451525071044542 20-21 0.03206343232990124 22-23 0.04057217488993976 24-25 0.033654498011859664 26-27 0.04811244268704706 28-29 0.04787032399631426 30-31 0.026805997902560372 32-33 0.032028843945510845 34-35 0.04821620784021827 36-37 0.009615570860531334 38-39 0.022759156928883514 40-41 0.04427313201971261 42-43 0.023312571079129922 44-45 0.017017485120077037 46-47 0.024350222610841937 48-49 0.030818250491846826 50-51 0.029746010575744414 52-53 0.02614881859914276 54-55 0.02262080339132191 56-57 0.02580293475523876 58-59 0.018401020495693053 60-61 0.02507657868304035 62-63 0.024211869073280337 64-65 0.02262080339132191 66-67 0.029849775728915614 68-69 0.022966687235225918 70-71 0.050187745750471094 72-73 0.023312571079129922 74-75 0.00947721732296973 76-77 0.017155838657638638 78-79 0.026287172136704364 80-81 0.019715379102528272 82-83 0.02666764436499877 84-85 0.012520995149324974 86-87 0.018366432111302653 88-89 0.024384810995232337 90-91 0.021929035703513903 92-93 0.00878544963516172 94-95 0.012071346152249767 96-97 0.015945245203974622 98-99 0.0072289723375937006 100-101 0.010687810776633748 102-103 0.003908487436115254 104-105 0.004461901586361661 106-107 0.0010376515317120144 108-109 0.015391831053728213 110-111 0.0014873005287872206 112-113 0.0024211869073280335 114-115 0.013904530524940992 116-117 0.0058108485775872805 118-119 0.2803042670998055 120-121 0.0014873005287872206 122-123 0.0012105934536640168 124-125 0.31575736109996594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1445572.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.90364369607802 #Duplication Level Percentage of deduplicated Percentage of total 1 85.53415563247995 60.64683294810243 2 8.447283111303856 11.978863038475719 3 2.459882429917512 5.2324388203534165 4 1.1200736467257275 3.17669211043231 5 0.6257070987825295 2.218245659509158 6 0.4009689889303436 1.7058097394576237 7 0.26463881086990726 1.313469916785158 8 0.19890250583946026 1.1282329923438534 9 0.14916662321641566 0.9518811393495469 >10 0.763783032899914 9.332507632208506 >50 0.02784388937747066 1.362393605804063 >100 0.00730216458770136 0.8136785124631079 >500 2.9206506920301776E-4 0.13895388471506404 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 6.917676878080096E-5 0.0 0.0 0.0 6 0.0 6.917676878080096E-5 0.0 0.0 6.917676878080096E-5 7 0.0 6.917676878080096E-5 0.0 0.0 6.917676878080096E-5 8 0.0 6.917676878080096E-5 0.0 0.0 6.917676878080096E-5 9 0.0 6.917676878080096E-5 0.0 0.0 6.917676878080096E-5 10-11 0.0 1.3835353756160192E-4 0.0 0.0 6.917676878080096E-5 12-13 0.0 1.3835353756160192E-4 0.0 6.917676878080096E-5 6.917676878080096E-5 14-15 0.0 1.729419219520024E-4 0.0 6.917676878080096E-5 1.0376515317120144E-4 16-17 0.0 2.0753030634240287E-4 0.0 6.917676878080096E-5 2.0753030634240287E-4 18-19 0.0 2.0753030634240287E-4 0.0 6.917676878080096E-5 2.7670707512320383E-4 20-21 0.0 2.0753030634240287E-4 0.0 6.917676878080096E-5 3.458838439040048E-4 22-23 0.0 2.0753030634240287E-4 0.0 6.917676878080096E-5 4.4964899707520623E-4 24-25 0.0 2.0753030634240287E-4 0.0 1.3835353756160192E-4 6.225909190272086E-4 26-27 0.0 2.0753030634240287E-4 0.0 4.842373814656067E-4 6.225909190272086E-4 28-29 0.0 2.0753030634240287E-4 0.0 0.0014527121443968201 6.225909190272086E-4 30-31 0.0 2.7670707512320383E-4 0.0 0.004600255123923263 6.917676878080096E-4 32-33 0.0 2.7670707512320383E-4 0.0 0.0143195911376258 7.609444565888105E-4 34-35 0.0 2.7670707512320383E-4 0.0 0.026252583752313964 7.609444565888105E-4 36-37 0.0 2.7670707512320383E-4 0.0 0.042924185028486994 7.609444565888105E-4 38-39 0.0 2.7670707512320383E-4 0.0 0.07170172084130019 7.609444565888105E-4 40-41 0.0 2.7670707512320383E-4 0.0 0.10995647397708312 7.609444565888105E-4 42-43 0.0 2.7670707512320383E-4 0.0 0.1448907422113876 7.95532840979211E-4 44-45 0.0 2.7670707512320383E-4 0.0 0.18276502311887613 8.64709609760012E-4 46-47 0.0 2.7670707512320383E-4 0.0 0.2225762535522271 8.992979941504125E-4 48-49 0.0 2.7670707512320383E-4 0.0 0.26781786033487087 8.992979941504125E-4 50-51 0.0 2.7670707512320383E-4 0.0 0.31852443185119805 8.992979941504125E-4 52-53 0.0 2.7670707512320383E-4 0.0 0.3723439579626612 9.684747629312134E-4 54-55 0.0 2.7670707512320383E-4 0.0 0.4298298528195067 0.0010376515317120144 56-57 0.0 2.7670707512320383E-4 0.0 0.4928498891788164 0.0010722399161024149 58-59 0.0 2.7670707512320383E-4 0.0 0.5667652666211023 0.0011414166848832158 60-61 0.0 2.7670707512320383E-4 0.0 0.6378789849277655 0.0011760050692736163 62-63 0.0 2.7670707512320383E-4 0.0 0.7188158044013028 0.0011760050692736163 64-65 0.0 2.7670707512320383E-4 0.0 0.796916376354827 0.0011760050692736163 66-67 0.0 3.458838439040048E-4 0.0 0.8892673626771963 0.0011760050692736163 68-69 0.0 3.458838439040048E-4 0.0 0.994000990611329 0.0011760050692736163 70-71 6.917676878080096E-5 3.8047222829440527E-4 0.0 1.1029198130567002 0.0012451818380544172 72-73 6.917676878080096E-5 4.1506061268480575E-4 0.0 1.2085873273693735 0.0013835353756160192 74-75 6.917676878080096E-5 4.1506061268480575E-4 0.0 1.3195814528781686 0.0014527121443968201 76-77 6.917676878080096E-5 4.1506061268480575E-4 0.0 1.4480081241197258 0.0014527121443968201 78-79 6.917676878080096E-5 4.1506061268480575E-4 0.0 1.5971532376111326 0.0014527121443968201 80-81 6.917676878080096E-5 4.1506061268480575E-4 0.0 1.7479240051688882 0.0014527121443968201 82-83 6.917676878080096E-5 4.1506061268480575E-4 0.0 1.90872540419986 0.001521888913177621 84-85 6.917676878080096E-5 4.1506061268480575E-4 0.0 2.074922591195734 0.001521888913177621 86-87 6.917676878080096E-5 4.1506061268480575E-4 0.0 2.2608697456785274 0.001521888913177621 88-89 1.0376515317120144E-4 4.1506061268480575E-4 0.0 2.47272360006973 0.001521888913177621 90-91 1.3835353756160192E-4 4.1506061268480575E-4 0.0 2.6918410151829173 0.001521888913177621 92-93 1.3835353756160192E-4 4.842373814656067E-4 0.0 2.9149360945010003 0.001521888913177621 94-95 1.3835353756160192E-4 5.534141502464077E-4 0.0 3.1432540198620336 0.001591065681958422 96-97 1.3835353756160192E-4 5.534141502464077E-4 0.0 3.4046730290846803 0.001591065681958422 98-99 1.3835353756160192E-4 5.534141502464077E-4 0.0 3.692067914984518 0.001591065681958422 100-101 1.3835353756160192E-4 5.534141502464077E-4 0.0 3.99537345770394 0.001591065681958422 102-103 1.3835353756160192E-4 6.225909190272086E-4 0.0 4.322441220499567 0.001798595988300825 104-105 1.3835353756160192E-4 6.225909190272086E-4 0.0 4.663897751201601 0.001798595988300825 106-107 1.3835353756160192E-4 6.225909190272086E-4 0.0 5.033370873259859 0.0018331843726912254 108-109 1.3835353756160192E-4 6.225909190272086E-4 0.0 5.421902195117227 0.0019023611414720264 110-111 1.3835353756160192E-4 6.225909190272086E-4 0.0 5.8312903127620075 0.0020407146790336283 112-113 1.3835353756160192E-4 6.225909190272086E-4 0.0 6.246039629987299 0.0020753030634240287 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2905 0.0 43.235737 1 GGTATCA 1795 0.0 37.473175 1 TCAACGC 3540 0.0 33.962093 4 GTACATG 8020 0.0 33.919415 1 CAACGCA 3655 0.0 33.055214 5 ATCAACG 3775 0.0 32.00556 3 TATACCG 205 6.91216E-11 31.935238 5 TACATGG 8410 0.0 31.917353 2 CATGGGG 6630 0.0 31.688927 4 ACATGGG 8360 0.0 31.538702 3 AACGCAG 3850 0.0 31.531206 6 TATCAAC 4025 0.0 30.60912 2 ATGGGGG 4310 0.0 26.512781 5 ACGCAGA 4755 0.0 25.523815 7 CGCAGAG 5005 0.0 24.011164 8 CTCTACG 480 0.0 23.559101 3 TGGGGGG 3205 0.0 21.90913 6 TCTACGT 355 7.8216544E-11 21.795225 4 ATTAACG 165 3.2093606E-4 21.64281 2 GAGTACA 5945 0.0 20.926708 1 >>END_MODULE