FastQCFastQC Report
Mon 15 Aug 2016
SRR2921581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445572
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26730.18490950295108097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20920.1447178002894356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16020.11082118358684313No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.046.3166541
GTATCAA42050.045.5090941
TCAACGC54600.035.041384
GTACATG82700.034.385241
CAACGCA55450.034.378865
ATCAACG56150.033.864123
TACATGG83900.033.462462
TATCAAC57900.033.252392
ACATGGG84050.032.9772263
AACGCAG58150.032.8941546
CATGGGG64700.032.427034
ATGGGGG39850.028.8517465
ACGCAGA69500.027.4364957
CGCAGAG72900.026.3194478
GCAGAGT81250.023.6154359
AGAGTAC75100.022.97058710-11
TATACTG7100.022.6541865
TATCACG1852.653929E-522.5540872
AGTACTT39350.021.93944712-13
GAGTACT36700.021.25238412-13
Produced by FastQC (version 0.11.5)