##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921581_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1445572
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.679476359531037	33.0	31.0	34.0	30.0	34.0
2	31.873267467825883	33.0	31.0	34.0	30.0	34.0
3	31.93277885847263	34.0	31.0	34.0	30.0	34.0
4	35.32146029391826	37.0	35.0	37.0	33.0	37.0
5	35.18753199425556	37.0	35.0	37.0	33.0	37.0
6	35.21603628183169	37.0	35.0	37.0	33.0	37.0
7	35.10882405027214	37.0	35.0	37.0	33.0	37.0
8	35.14866156787763	37.0	35.0	37.0	33.0	37.0
9	36.857630751010674	39.0	38.0	39.0	34.0	39.0
10-11	36.80971027385699	39.0	38.0	39.0	33.0	39.0
12-13	36.74256626442681	39.0	38.0	39.0	33.0	39.0
14-15	38.15174615999757	41.0	39.0	41.0	33.0	41.0
16-17	38.16223992993777	41.0	39.0	41.0	33.0	41.0
18-19	38.18502191520035	41.0	39.0	41.0	33.5	41.0
20-21	38.12721331071714	41.0	39.0	41.0	34.0	41.0
22-23	38.12638457302715	41.0	39.0	41.0	34.0	41.0
24-25	38.08679436237005	41.0	39.0	41.0	33.5	41.0
26-27	38.01248467734571	41.0	39.0	41.0	33.0	41.0
28-29	37.94436631312726	41.0	38.5	41.0	33.0	41.0
30-31	37.81918299468999	41.0	38.0	41.0	33.0	41.0
32-33	37.68616021893064	41.0	38.0	41.0	33.0	41.0
34-35	37.554129092151754	40.0	38.0	41.0	32.0	41.0
36-37	37.47252610039486	40.0	38.0	41.0	32.0	41.0
38-39	37.39505676645646	40.0	38.0	41.0	32.0	41.0
40-41	37.2739725174533	40.0	38.0	41.0	32.0	41.0
42-43	37.11144930864737	40.0	37.5	41.0	31.0	41.0
44-45	36.94469524866281	40.0	37.0	41.0	31.0	41.0
46-47	36.722340706654535	40.0	37.0	41.0	30.0	41.0
48-49	36.5201114161038	40.0	37.0	41.0	30.0	41.0
50-51	36.27730718359238	40.0	36.0	41.0	29.0	41.0
52-53	36.16324195543356	40.0	36.0	41.0	29.0	41.0
54-55	36.0493718057627	40.0	35.0	41.0	29.0	41.0
56-57	35.85962200430002	39.0	35.0	41.0	29.0	41.0
58-59	35.6760514177087	39.0	35.0	41.0	28.5	41.0
60-61	35.43210369320933	39.0	35.0	41.0	28.0	41.0
62-63	35.09691907424881	38.0	35.0	41.0	28.0	41.0
64-65	34.82005427609279	37.0	35.0	40.0	28.0	41.0
66-67	34.52351387547628	37.0	35.0	40.0	28.0	41.0
68-69	34.162258953549184	36.0	35.0	39.0	27.0	41.0
70-71	33.79505171655234	36.0	34.5	39.0	26.0	41.0
72-73	33.47699353612272	35.0	34.0	38.5	26.0	40.5
74-75	33.15430189572017	35.0	34.0	37.0	26.0	39.5
76-77	32.82569391216764	35.0	34.0	37.0	26.0	39.0
78-79	32.55437847440321	35.0	34.0	36.0	26.0	39.0
80-81	32.290144316575024	35.0	34.0	36.0	26.0	37.0
82-83	32.05062390527763	35.0	34.0	35.5	25.0	37.0
84-85	31.825640991939522	35.0	34.0	35.0	24.5	37.0
86-87	31.689265218197363	35.0	34.0	35.0	24.5	36.0
88-89	31.577986776168878	35.0	33.5	35.0	24.0	36.0
90-91	31.46229312687296	35.0	33.0	35.0	24.0	36.0
92-93	31.373359472928364	35.0	33.0	35.0	24.0	35.5
94-95	31.302537334702112	35.0	33.0	35.0	23.5	35.0
96-97	31.235368421635172	35.0	33.0	35.0	23.0	35.0
98-99	31.15990106338529	35.0	33.0	35.0	23.0	35.0
100-101	31.08815679883119	35.0	33.0	35.0	20.0	35.0
102-103	30.408557650535567	34.0	31.5	35.0	19.5	35.0
104-105	30.917590061235273	35.0	33.0	35.0	20.0	35.0
106-107	31.018184151325563	35.0	33.0	35.0	20.0	35.0
108-109	31.007347610496055	35.0	33.0	35.0	20.0	35.0
110-111	30.94739902267061	35.0	33.0	35.0	19.0	35.0
112-113	30.912544653604247	35.0	33.0	35.0	19.0	35.0
114-115	30.79943890722842	35.0	33.0	35.0	18.0	35.0
116-117	30.984816736904143	35.0	33.0	35.0	19.0	35.0
118-119	30.97416316862806	35.0	33.0	35.0	18.5	35.0
120-121	30.902197884297706	35.0	33.0	35.0	17.5	35.0
122-123	30.77201377724527	35.0	33.0	35.0	4.5	35.0
124-125	30.130365004302796	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23972.0
3	6421.0
4	3331.0
5	2654.0
6	2827.0
7	3169.0
8	3537.0
9	3769.0
10	4166.0
11	4727.0
12	5165.0
13	6708.0
14	9608.0
15	8492.0
16	4602.0
17	4024.0
18	3618.0
19	3137.0
20	3075.0
21	2940.0
22	3404.0
23	4221.0
24	5366.0
25	7183.0
26	8599.0
27	10072.0
28	12252.0
29	14643.0
30	17750.0
31	22070.0
32	28900.0
33	39127.0
34	60851.0
35	108933.0
36	246865.0
37	530027.0
38	214892.0
39	475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88455350933944	18.849859709454993	12.040284663527572	26.22530211767799
2	15.66002350667256	20.20902813952089	38.157389555649175	25.973558798157377
3	18.578976323192062	24.552604172072922	28.570706913434158	28.297712591300854
4	12.992382693268457	15.921300534036051	35.23085781835845	35.855458954337045
5	14.449261964533333	37.73654361923847	32.596243533713256	15.217950882514938
6	33.29761207544662	35.03403903585833	17.06929231757583	14.599056571119224
7	29.731321672010058	31.91664313360687	19.71772786923371	18.634307325149365
8	27.7369711625306	34.900556512567384	18.635246534167628	18.727225790734387
9	27.680530691134408	15.943432067479598	18.79196627957751	37.58407096180848
10-11	26.421080101867407	25.744225309994373	26.416511435855583	21.418183152282637
12-13	26.71830543799108	23.011139563464226	26.998360576874823	23.272194421669873
14-15	24.04522412188515	24.216128026336783	24.742406837817455	26.996241013960613
16-17	23.935519125304843	27.224935207317554	25.23909010275121	23.600455564626397
18-19	23.895647780984213	26.299056753932497	27.202164690901792	22.603130774181494
20-21	24.809326292576586	25.230581554375174	27.10052452784364	22.859567625204598
22-23	24.46963169525886	24.836556318329247	27.03806946732631	23.655742519085578
24-25	23.98365406500325	25.117416362676643	27.089160337240408	23.8097692350797
26-27	23.6695746076943	25.387878240735457	27.175015020499483	23.767532131070766
28-29	23.827645737684445	25.71360280650391	27.028027335879397	23.430724119932243
30-31	24.391436809598655	25.339251322692757	26.9150681823213	23.35424368538729
32-33	24.007412280974847	25.330505126816146	26.95904150918856	23.70304108302045
34-35	24.098011692831758	25.55079519142498	27.017138002671736	23.33405511307152
36-37	24.148081631410616	25.37684896224281	27.010397288638387	23.464672117708183
38-39	24.127194077779983	25.56790235337822	26.877517659502786	23.42738590933901
40-41	24.18856302429157	25.329569525749175	26.896615893774012	23.585251556185238
42-43	24.06635286825309	25.323137652073935	26.71717465206148	23.893334827611493
44-45	23.931456006419946	25.433190706297843	26.73098142850719	23.904371858775022
46-47	23.9939536387991	25.49551279057902	26.470090747303267	24.040442823318614
48-49	24.02204994477829	25.709693252297676	26.31583498583398	23.952421817090055
50-51	24.091934812174777	25.89398746217692	26.226387814708076	23.787689910940227
52-53	24.215302475101293	25.75384436587419	26.083129202099954	23.947723956924563
54-55	23.966988459658616	25.52725036311103	26.398889791694124	24.10687138553623
56-57	24.084134897249125	25.712155051383217	26.070078748055685	24.13363130331197
58-59	24.02484989376086	25.68327350067569	26.295216488840477	23.99666011672297
60-61	24.245082308277382	25.685654428023796	26.37444775301671	23.69481551068211
62-63	24.135549181232278	25.760667258595564	26.31751162960424	23.786271930567914
64-65	23.939866018434223	25.458399858325976	26.414526568029817	24.18720755520998
66-67	23.99215920128176	25.662081914001643	26.308875169835545	24.036883714881053
68-69	23.895542392667217	25.58177180163431	26.380076959661032	24.14260884603744
70-71	23.862906863165584	25.494302601323216	26.514556175686856	24.128234359824347
72-73	24.0808183247973	25.809067001674464	26.107398730646054	24.002715942882187
74-75	23.89141705188676	25.872435305853912	26.103429000743	24.132718641516327
76-77	23.887858135068875	25.483174500395698	26.19909874759952	24.429868616935906
78-79	23.847910407597286	25.596944870843245	26.29141189386325	24.26373282769622
80-81	23.95657220809479	25.794875661675793	26.042701435833017	24.205850694396407
82-83	24.11021337445442	25.744091783599576	26.091379583489854	24.054315258456146
84-85	23.96455035770283	25.919247673971764	25.83146062363395	24.28474134469146
86-87	23.771489763221755	25.94146123472231	26.00444668269723	24.282602319358702
88-89	23.739316823186655	25.712572087339435	26.048221600623428	24.499889488850478
90-91	23.928321154653666	25.608605390717365	26.143727097960028	24.31934635666894
92-93	23.92153417470731	25.8060131214495	26.068954019585327	24.203498684257855
94-95	23.780025485603122	25.69653038833031	26.050139672727223	24.47330445333935
96-97	23.634362513420477	25.652968554572926	26.15346940130829	24.559199530698308
98-99	23.60698786321044	25.620700854718592	25.98356972903646	24.78874155303451
100-101	23.566138525095948	25.771978151209346	26.055153254213558	24.60673006948115
102-103	23.585107972594553	25.55356016248464	26.167789190565266	24.69354267435554
104-105	23.693112484193108	25.66575722274643	25.940043110962304	24.701087182098156
106-107	23.67086111956612	25.524156728188384	26.101649187857994	24.703332964387503
108-109	23.676104806834562	25.480764740817502	26.06829787300277	24.774832579345162
110-111	23.51399550765753	25.825504247110658	26.04036743957924	24.620132805652574
112-113	23.432154995237163	25.846190456755032	26.066934289451822	24.654720258555983
114-115	23.437469735163656	25.91475900197292	25.953152108646265	24.694619154217154
116-117	23.36217368686114	25.78766194595253	26.01280210080081	24.837362266385526
118-119	23.406208514379838	25.61349427995664	26.08429013283008	24.89600707283344
120-121	23.235217207228896	25.621812592180593	26.25694208083944	24.886028119751067
122-123	23.176097637746253	25.75612354488524	26.041102073781737	25.026676743586773
124-125	23.064920818388888	25.757656553960327	25.987579979763968	25.189842647886817
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	33.0
1	26.0
2	32.0
3	57.5
4	54.5
5	57.0
6	117.5
7	394.0
8	674.0
9	733.5
10	663.0
11	533.0
12	534.5
13	593.0
14	650.5
15	731.0
16	812.5
17	926.5
18	1002.5
19	1026.0
20	1076.5
21	1179.0
22	1370.5
23	1574.5
24	1828.5
25	2258.0
26	2869.5
27	3813.0
28	5013.0
29	6512.5
30	8456.0
31	10820.0
32	13668.0
33	16352.0
34	18913.0
35	21803.5
36	25121.5
37	28251.5
38	30733.0
39	33290.0
40	35824.0
41	37901.5
42	39954.5
43	43337.5
44	46858.0
45	49787.5
46	52674.5
47	55270.0
48	58699.5
49	61844.0
50	63231.0
51	63449.0
52	62189.0
53	60760.0
54	60136.0
55	58169.0
56	54115.5
57	49361.5
58	45115.5
59	40121.0
60	34028.5
61	28832.0
62	23979.0
63	18464.0
64	13701.0
65	10411.5
66	7806.5
67	5848.0
68	4493.5
69	3394.5
70	2688.0
71	2130.5
72	1625.0
73	1362.5
74	1115.5
75	848.5
76	602.5
77	362.0
78	211.0
79	121.0
80	80.0
81	40.5
82	21.5
83	19.0
84	17.0
85	8.5
86	4.5
87	5.5
88	4.5
89	2.5
90	2.0
91	2.0
92	0.5
93	0.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02469610645474594
2	0.001729419219520024
3	0.03368908639625007
4	0.1667851895305111
5	0.026563879211827564
6	0.07291231429496421
7	0.055064707949517566
8	0.04710937953972545
9	0.03548768238455089
10-11	0.06564875357298011
12-13	0.05924990246075602
14-15	0.06229368028711126
16-17	0.05336987711438794
18-19	0.03887734405481014
20-21	0.025422462526944353
22-23	0.0030091894419648417
24-25	0.005188257658560072
26-27	0.0032858965170880455
28-29	0.00501531573660807
30-31	0.0024903636761088345
32-33	0.009200510247846527
34-35	0.004911550583436868
36-37	0.006848500109299294
38-39	0.0022828333697664316
40-41	0.003458838439040048
42-43	0.004012252589286456
44-45	0.005326611196121674
46-47	0.005153669274169671
48-49	0.002455775291718434
50-51	0.0017985959883008247
52-53	0.0010376515317120144
54-55	0.005361199580512074
56-57	8.64709609760012E-4
58-59	8.64709609760012E-4
60-61	3.458838439040048E-5
62-63	0.0010722399161024149
64-65	0.0
66-67	0.0038738990517248537
68-69	6.571793034176091E-4
70-71	0.0
72-73	0.002317421754156832
74-75	0.005395787964902475
76-77	0.0019369495258624268
78-79	2.0753030634240285E-4
80-81	0.0
82-83	0.0058800253463680814
84-85	0.0019715379102528273
86-87	0.0
88-89	0.0011414166848832158
90-91	8.992979941504123E-4
92-93	0.0
94-95	0.003458838439040048
96-97	0.0013835353756160192
98-99	5.880025346368081E-4
100-101	0.0
102-103	0.0013835353756160192
104-105	0.0
106-107	8.301212253696114E-4
108-109	0.002524952060499235
110-111	6.917676878080096E-5
112-113	3.458838439040048E-4
114-115	0.0
116-117	0.0019369495258624268
118-119	0.0028362475200128393
120-121	0.0027670707512320383
122-123	0.0016948308351296235
124-125	0.11158212804343193
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1445572.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.60617822248996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.41250184939301	56.392004660138376
2	9.775241201715081	13.217333977019537
3	2.7125126952949343	5.501478501266279
4	1.2876585559190572	3.4821469528471143
5	0.7432736994453485	2.512494709639584
6	0.4551341946714311	1.8461930088063732
7	0.3059976658243111	1.4481132911969012
8	0.2208604570356508	1.1945225136522237
9	0.1718423306067439	1.0455842906250814
>10	0.8695838486233761	10.126177345762084
>50	0.033897125791026834	1.5444969059581
>100	0.010880529488075356	1.1133641238781202
>500	3.079230959778375E-4	0.13427289890046576
>1k	3.079230959778375E-4	0.44181682030969793
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2673	0.18490950295108097	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2092	0.1447178002894356	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1602	0.11082118358684313	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.917676878080096E-5
7	0.0	0.0	0.0	0.0	6.917676878080096E-5
8	0.0	0.0	0.0	0.0	6.917676878080096E-5
9	0.0	0.0	0.0	0.0	6.917676878080096E-5
10-11	0.0	0.0	0.0	0.0	6.917676878080096E-5
12-13	0.0	0.0	0.0	0.0	2.0753030634240287E-4
14-15	0.0	0.0	0.0	0.0	3.112954595136043E-4
16-17	0.0	0.0	0.0	0.0	3.458838439040048E-4
18-19	0.0	0.0	0.0	0.0	4.1506061268480575E-4
20-21	0.0	0.0	0.0	0.0	4.1506061268480575E-4
22-23	0.0	0.0	0.0	0.0	4.1506061268480575E-4
24-25	0.0	0.0	0.0	1.0376515317120144E-4	4.1506061268480575E-4
26-27	0.0	0.0	0.0	4.842373814656067E-4	4.1506061268480575E-4
28-29	0.0	0.0	0.0	0.001521888913177621	4.4964899707520623E-4
30-31	0.0	0.0	0.0	0.004600255123923263	5.188257658560072E-4
32-33	0.0	0.0	0.0	0.014146649215673796	6.225909190272086E-4
34-35	0.0	0.0	0.0	0.025699169602067556	7.609444565888105E-4
36-37	0.0	0.0	0.0	0.04247453603141178	7.609444565888105E-4
38-39	0.0	0.0	0.0	0.07097536476910178	7.609444565888105E-4
40-41	0.0	0.0	0.0	0.10940305982683671	7.609444565888105E-4
42-43	0.0	0.0	0.0	0.145271214439682	7.609444565888105E-4
44-45	0.0	3.458838439040048E-5	0.0	0.18328384888473215	8.301212253696115E-4
46-47	0.0	6.917676878080096E-5	0.0	0.22361390508393908	9.338863785408129E-4
48-49	0.0	6.917676878080096E-5	0.0	0.26885551186658296	0.0010376515317120144
50-51	0.0	6.917676878080096E-5	0.0	0.3198042020736428	0.0010376515317120144
52-53	0.0	1.0376515317120144E-4	0.0	0.37338160949437316	0.0011414166848832158
54-55	0.0	1.3835353756160192E-4	0.0	0.43149009527024595	0.0011760050692736163
56-57	0.0	1.3835353756160192E-4	0.0	0.49447554324516524	0.0011760050692736163
58-59	0.0	2.0753030634240287E-4	0.0	0.5685638626094031	0.0011760050692736163
60-61	0.0	2.7670707512320383E-4	0.0	0.6403001718350936	0.0011760050692736163
62-63	0.0	2.7670707512320383E-4	0.0	0.722551349915466	0.0012451818380544172
64-65	0.0	2.7670707512320383E-4	0.0	0.8005827451002094	0.0012451818380544172
66-67	0.0	3.458838439040048E-4	0.0	0.893210438497702	0.0012451818380544172
68-69	0.0	3.458838439040048E-4	0.0	0.9982553618913481	0.0012451818380544172
70-71	0.0	3.458838439040048E-4	0.0	1.107831363640137	0.0013489469912256187
72-73	0.0	3.8047222829440527E-4	0.0	1.2139485269498855	0.0014527121443968201
74-75	0.0	4.1506061268480575E-4	0.0	1.3261532459123448	0.0014527121443968201
76-77	0.0	4.1506061268480575E-4	0.0	1.4573469879051337	0.0014527121443968201
78-79	0.0	4.1506061268480575E-4	0.0	1.6077026948502047	0.0014527121443968201
80-81	0.0	4.1506061268480575E-4	0.0	1.7605141770869939	0.0014527121443968201
82-83	0.0	4.1506061268480575E-4	0.0	1.921419341271137	0.001591065681958422
84-85	0.0	4.1506061268480575E-4	0.0	2.0895534777928737	0.0016256540663488225
86-87	0.0	4.1506061268480575E-4	0.0	2.278544410102022	0.001660242450739223
88-89	0.0	4.1506061268480575E-4	0.0	2.495482756998614	0.001660242450739223
90-91	0.0	4.1506061268480575E-4	0.0	2.7181627757040117	0.001660242450739223
92-93	0.0	4.1506061268480575E-4	0.0	2.943263981316738	0.001660242450739223
94-95	0.0	4.1506061268480575E-4	0.0	3.174279800660223	0.001660242450739223
96-97	0.0	4.1506061268480575E-4	0.0	3.438742587709225	0.0016948308351296235
98-99	0.0	4.1506061268480575E-4	0.0	3.7296309004324932	0.001729419219520024
100-101	0.0	4.1506061268480575E-4	0.0	4.036014809362661	0.001729419219520024
102-103	0.0	4.842373814656067E-4	0.0	4.364085635305609	0.001729419219520024
104-105	0.0	4.842373814656067E-4	0.0	4.707444527149114	0.001729419219520024
106-107	0.0	5.534141502464077E-4	0.0	5.076364235057126	0.0017640076039104244
108-109	0.0	5.534141502464077E-4	0.0	5.466970859977919	0.001798595988300825
110-111	0.0	5.534141502464077E-4	0.0	5.880025346368081	0.0018331843726912254
112-113	0.0	5.534141502464077E-4	0.0	6.30148481016511	0.0018677727570816259
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2600	0.0	46.316654	1
GTATCAA	4205	0.0	45.509094	1
TCAACGC	5460	0.0	35.04138	4
GTACATG	8270	0.0	34.38524	1
CAACGCA	5545	0.0	34.37886	5
ATCAACG	5615	0.0	33.86412	3
TACATGG	8390	0.0	33.46246	2
TATCAAC	5790	0.0	33.25239	2
ACATGGG	8405	0.0	32.977226	3
AACGCAG	5815	0.0	32.894154	6
CATGGGG	6470	0.0	32.42703	4
ATGGGGG	3985	0.0	28.851746	5
ACGCAGA	6950	0.0	27.436495	7
CGCAGAG	7290	0.0	26.319447	8
GCAGAGT	8125	0.0	23.615435	9
AGAGTAC	7510	0.0	22.970587	10-11
TATACTG	710	0.0	22.654186	5
TATCACG	185	2.653929E-5	22.554087	2
AGTACTT	3935	0.0	21.939447	12-13
GAGTACT	3670	0.0	21.252384	12-13
>>END_MODULE