FastQCFastQC Report
Mon 15 Aug 2016
SRR2921580_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921580_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187038
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30000.035.9150661
GTACATG70550.034.594431
TACATGG71950.033.335122
GGTATCA21550.033.1476941
ACATGGG73700.032.059063
ATGGGGG35650.030.5479955
TCAACGC35300.030.3476834
CATGGGG59200.029.7575914
ATCAACG36750.029.6361333
CAACGCA36100.029.5078095
TATCAAC37150.029.1568322
GTATAGG2950.028.2504481
AACGCAG38350.028.2385396
CTAGCGA1358.232106E-526.451194
TGGGGGG29000.024.8269636
ACGCAGA43750.024.6119047
CGCAGAG46200.023.4335168
ATACCGG1350.002554673522.0380126
TATCACG1400.003151053621.2554232
GTATAGT2858.0061E-820.8869231
Produced by FastQC (version 0.11.5)