##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921580_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1187038
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.979820359584107	31.0	30.0	33.0	23.0	34.0
2	29.214787563666874	31.0	30.0	34.0	23.0	34.0
3	29.53333675922759	31.0	31.0	34.0	24.0	34.0
4	33.02686855854657	37.0	35.0	37.0	26.0	37.0
5	33.11467366672339	37.0	35.0	37.0	26.0	37.0
6	32.946935986885	37.0	35.0	37.0	25.0	37.0
7	32.92314989073644	37.0	35.0	37.0	25.0	37.0
8	32.691502715161604	37.0	35.0	37.0	23.0	37.0
9	34.43463899218054	39.0	35.0	39.0	24.0	39.0
10-11	34.4695995410425	39.0	36.0	39.0	25.0	39.0
12-13	34.40330932960866	39.0	35.0	39.0	23.0	39.0
14-15	35.630790252713055	40.0	37.0	41.0	23.0	41.0
16-17	35.59838269709984	40.0	37.0	41.0	22.5	41.0
18-19	35.55417476104388	40.0	37.0	41.0	22.0	41.0
20-21	35.60901209565321	40.0	37.0	41.0	22.5	41.0
22-23	35.54502088391442	40.0	37.0	41.0	22.0	41.0
24-25	35.54419909050932	40.0	37.0	41.0	23.0	41.0
26-27	35.50297420975571	40.0	37.0	41.0	23.0	41.0
28-29	35.445786065820975	40.0	36.0	41.0	23.0	41.0
30-31	35.3423365553588	40.0	36.0	41.0	22.0	41.0
32-33	35.246751157081746	40.0	36.0	41.0	21.5	41.0
34-35	35.11347699062709	40.0	36.0	41.0	19.5	41.0
36-37	35.00700777902645	40.0	35.0	41.0	18.0	41.0
38-39	34.88096463634694	40.0	35.0	41.0	16.5	41.0
40-41	34.76206069224406	40.0	35.0	41.0	15.5	41.0
42-43	34.516786320235745	40.0	35.0	41.0	14.0	41.0
44-45	34.16412827559017	39.0	34.5	41.0	9.0	41.0
46-47	33.896464140153896	39.0	34.0	41.0	8.0	41.0
48-49	33.694189655259564	39.0	34.0	40.5	6.5	41.0
50-51	33.49426934942268	38.5	33.0	40.0	2.0	41.0
52-53	33.29938005354504	38.0	33.0	40.0	2.0	41.0
54-55	33.05783429005643	38.0	33.0	40.0	2.0	41.0
56-57	32.81509437777055	38.0	33.0	40.0	2.0	41.0
58-59	32.84190775695471	38.0	33.0	40.0	2.0	41.0
60-61	32.641691757129934	37.0	33.0	40.0	2.0	41.0
62-63	32.33692855662582	37.0	32.0	40.0	2.0	41.0
64-65	32.04753175551246	36.0	32.0	39.5	2.0	41.0
66-67	31.732686316697528	36.0	32.0	39.0	2.0	41.0
68-69	31.405962151169547	35.0	31.5	39.0	2.0	40.5
70-71	31.047263861814027	35.0	31.0	38.0	2.0	40.0
72-73	30.708016087100834	35.0	31.0	37.0	2.0	39.5
74-75	30.345747987848746	35.0	31.0	37.0	2.0	39.0
76-77	30.047628635309064	35.0	31.0	36.0	2.0	39.0
78-79	29.779583298933986	35.0	30.0	36.0	2.0	37.5
80-81	29.52763348772322	35.0	30.0	35.0	2.0	37.0
82-83	29.298375030959413	35.0	30.0	35.0	2.0	36.5
84-85	29.08619900963575	34.0	30.0	35.0	2.0	36.0
86-87	28.918428896126322	34.0	30.0	35.0	2.0	36.0
88-89	28.758257949619136	34.0	29.5	35.0	2.0	35.5
90-91	28.570507852318123	34.0	29.0	35.0	2.0	35.0
92-93	28.496673232027955	34.0	29.0	35.0	2.0	35.0
94-95	28.379111283716277	34.0	29.0	35.0	2.0	35.0
96-97	28.30636592931313	34.0	29.0	35.0	2.0	35.0
98-99	28.222283532624903	34.0	29.0	35.0	2.0	35.0
100-101	28.12705911689432	34.0	29.0	35.0	2.0	35.0
102-103	27.27637068063533	32.5	26.5	34.5	2.0	35.0
104-105	28.180059947533273	34.0	29.0	35.0	2.0	35.0
106-107	28.456697258217513	34.0	30.0	35.0	2.0	35.0
108-109	28.461546723862252	34.0	30.0	35.0	2.0	35.0
110-111	28.41671580859248	34.0	30.0	35.0	2.0	35.0
112-113	28.35141966811509	34.0	30.0	35.0	2.0	35.0
114-115	28.249698830197516	34.0	29.5	35.0	2.0	35.0
116-117	28.136914740724393	34.0	29.0	35.0	2.0	35.0
118-119	27.873887777813344	34.0	29.0	35.0	2.0	35.0
120-121	27.768358300239758	34.0	29.0	35.0	2.0	35.0
122-123	27.535002670512654	34.0	29.0	35.0	2.0	35.0
124-125	26.778112410891648	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68438.0
3	10764.0
4	5355.0
5	4419.0
6	4459.0
7	4826.0
8	5049.0
9	5327.0
10	5590.0
11	5741.0
12	5808.0
13	5862.0
14	6086.0
15	5655.0
16	4606.0
17	4185.0
18	3747.0
19	3583.0
20	3404.0
21	3505.0
22	4047.0
23	5360.0
24	7799.0
25	9924.0
26	10775.0
27	12073.0
28	14368.0
29	16866.0
30	20398.0
31	25518.0
32	33283.0
33	45764.0
34	71172.0
35	115063.0
36	219725.0
37	323550.0
38	84888.0
39	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30622509502756	17.95096063992666	13.14266309728749	24.600151167758295
2	16.654885463961737	19.38750664535659	39.290802489470636	24.666805401211032
3	19.61635899954758	24.527968065863316	27.91684183654986	27.938831098039245
4	13.422690435689594	15.7643756166154	34.82736453839044	35.98556940930456
5	14.627662711673045	37.38935033289689	32.67343750789903	15.309549447531035
6	32.97747565054837	34.17751511221701	17.44576760955883	15.399241627675783
7	29.471690511131023	31.738691756106235	20.472719701880273	18.316898030882466
8	27.046015597279034	34.65678990702821	19.790153975300235	18.507040520392522
9	27.619146331084266	16.77128336843028	18.542393990796427	37.06717630968903
10-11	26.210807961063658	25.770713376338396	26.569103868306236	21.44937479429171
12-13	26.12608931199884	23.348159407114323	26.773378449084273	23.75237283180256
14-15	23.958429450272202	24.426026507840405	24.122075780385277	27.49346826150212
16-17	24.381166112013293	27.37304982947464	24.509976597532333	23.735807460979736
18-19	24.415682374605478	26.643434143381473	25.923029273822117	23.01785420819094
20-21	25.218466646442174	25.586552398287658	25.580779991236053	23.61420096403411
22-23	25.16111280129379	25.12280979714156	25.53137517476536	24.184702226799292
24-25	24.62222926342765	25.414238469054673	25.480053140501685	24.483479127015993
26-27	24.279188544555037	25.587479245855494	25.63876410649721	24.494568103092263
28-29	24.547530523174352	26.03583683834148	25.426658626323363	23.989974012160808
30-31	25.139794025006196	25.580874087631223	25.49298670482142	23.786345182541165
32-33	24.548293981059167	25.653758693784546	25.49335528785607	24.304592037300218
34-35	24.58028520378923	25.942756969962577	25.554183663149377	23.922774163098808
36-37	24.704477335293586	25.964837636829703	25.38982115513018	23.940863872746533
38-39	24.827844510046212	25.94421677625495	25.302738773187013	23.925199940511828
40-41	24.683367746829042	25.74396359192617	25.475622603345833	24.09704605789895
42-43	24.621410647688606	25.686819899997808	25.387478997686163	24.30429045462742
44-45	24.398573022233755	25.704768276201424	25.62695687049014	24.269701831074684
46-47	24.290365024948294	25.708181430953076	25.57175894424668	24.42969459985195
48-49	24.26446175133692	25.961209489989734	25.468954614631382	24.30537414404196
50-51	24.309497947685575	25.870706297837128	25.490115942493063	24.329679811984235
52-53	24.49251817159922	25.88549153902028	25.325513043273094	24.296477246107408
54-55	24.29245283018868	25.48113544594768	25.706241110369604	24.520170613494038
56-57	24.356545270925313	25.72858300291092	25.339996974902224	24.574874751261547
58-59	24.326972781477906	25.849780990469917	25.543289823524283	24.279956404527898
60-61	24.70608061848938	25.703794988466523	25.541378329699498	24.048746063344602
62-63	24.585311226710292	25.860038668192946	25.48768141251395	24.06696869258281
64-65	24.366241865132782	25.55988235487422	25.544336211265772	24.529539568727227
66-67	24.44473469662912	25.808044526557723	25.423156085218977	24.32406469159418
68-69	24.263521865781122	25.634435856190326	25.64762298790913	24.454419290119425
70-71	24.30501357777257	25.638264876974088	25.60396224856679	24.45275929668655
72-73	24.295624074436866	26.03541944467265	25.30756607704477	24.36139040384571
74-75	24.35655376185096	25.899864271578167	25.27577470935498	24.46780725721589
76-77	24.249988204049718	25.55584202863383	25.505035185634554	24.689134581681902
78-79	24.18873923160636	25.564346615984768	25.72507991373067	24.521834238678203
80-81	24.3623306612112	25.79333716429254	25.34853583772461	24.495796336771647
82-83	24.381481478360595	25.891828671148946	25.39787334621448	24.328816504275974
84-85	24.411414185711163	25.927180369899016	25.244430514499427	24.41697492989039
86-87	24.179852362893055	26.021861616121615	25.35828666134717	24.43999935963816
88-89	24.15749154110511	25.72009621084483	25.51622325668401	24.60618899136605
90-91	24.508566435481466	25.595580657963584	25.44774402734114	24.448108879213805
92-93	24.42878614528378	25.764188092802875	25.461857573975564	24.345168187937784
94-95	24.307357629597675	25.717033559580223	25.452100591117734	24.523508219704375
96-97	24.123295185021977	25.753606789012007	25.49199188783166	24.631106138134353
98-99	24.04444485632973	25.789078599507647	25.353467783924227	24.813008760238393
100-101	23.985427787043147	25.843309112309083	25.49711795227381	24.674145148373956
102-103	23.738457669591853	25.78472682361554	25.704776663989026	24.77203884280358
104-105	24.03183683097583	25.809989974641738	25.5025316135772	24.65564158080523
106-107	23.965924953686816	25.76344093268308	25.635095073658814	24.63553903997129
108-109	23.93406048064856	25.728772731428023	25.62219036453276	24.71497642339066
110-111	23.756381526848745	26.099560243298342	25.610014995535035	24.53404323431787
112-113	23.778693993637784	26.103615139914808	25.53765433762873	24.580036528818678
114-115	23.83131564746042	26.202322982240407	25.441378000041286	24.524983370257893
116-117	23.81038634878765	25.98213183720081	25.49964342025061	24.707838393760927
118-119	23.789315846717564	25.73087929377726	25.70929557238044	24.77050928712474
120-121	23.704025704662595	25.94357981570687	25.742446222563693	24.609948257066844
122-123	23.638450586758548	26.031776787444294	25.636041212100793	24.69373141369637
124-125	23.52146573586366	25.911880752202855	25.701841634477006	24.86481187745648
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	347.0
1	289.5
2	183.0
3	145.5
4	140.5
5	117.0
6	113.5
7	139.5
8	180.0
9	197.0
10	190.0
11	203.0
12	240.0
13	267.5
14	292.5
15	336.5
16	376.5
17	429.5
18	472.0
19	496.0
20	572.5
21	671.0
22	802.5
23	987.5
24	1210.5
25	1531.5
26	1987.5
27	2613.0
28	3380.5
29	4469.0
30	5957.0
31	7472.5
32	9354.0
33	11496.0
34	13477.5
35	15422.0
36	17710.5
37	20203.0
38	22365.5
39	24775.0
40	27335.0
41	29609.0
42	32053.5
43	35004.0
44	38195.0
45	41562.0
46	44467.0
47	47231.5
48	50606.5
49	53311.0
50	54902.5
51	54592.0
52	52793.5
53	51508.0
54	50720.5
55	48761.0
56	45551.0
57	41482.0
58	37415.5
59	33930.5
60	29201.0
61	24514.0
62	20589.5
63	15955.0
64	11913.0
65	9263.5
66	7172.0
67	5541.0
68	4354.5
69	3434.5
70	2777.0
71	2255.0
72	1765.5
73	1404.5
74	1131.5
75	897.0
76	667.5
77	454.5
78	318.0
79	222.5
80	153.5
81	110.5
82	78.0
83	61.0
84	53.0
85	47.0
86	37.5
87	32.0
88	29.5
89	27.0
90	21.5
91	19.0
92	16.0
93	12.5
94	8.5
95	9.0
96	8.0
97	3.5
98	1.5
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023335394486107438
2	0.01002495286587287
3	0.008003113632419519
4	0.009687979660297311
5	0.015500767456475698
6	0.09073003560121917
7	0.05635876863251219
8	0.04624957246524543
9	0.02737907295301414
10-11	0.04957718287030406
12-13	0.013900144729991796
14-15	0.028010897713468312
16-17	0.034623996872888654
18-19	0.04068951457324871
20-21	0.0301591018990125
22-23	0.03765675572306868
24-25	0.0313806297692239
26-27	0.044480463135973744
28-29	0.043932881676913456
30-31	0.02459904400701578
32-33	0.02885333072740721
34-35	0.04363803012203485
36-37	0.009730101310994256
38-39	0.020850217094987692
40-41	0.041110731080218156
42-43	0.022240231567986872
44-45	0.016343200470414595
46-47	0.02388297594516772
48-49	0.02999061529622472
50-51	0.027716046158589698
52-53	0.02493601721259134
54-55	0.02207174496519909
56-57	0.025230868767469956
58-59	0.018322918053171003
60-61	0.0232090295340166
62-63	0.023124786232622714
64-65	0.020934460396381582
66-67	0.02784241111068053
68-69	0.023166907883319657
70-71	0.04549138275270042
72-73	0.021945380013108258
74-75	0.009182519851933973
76-77	0.016006227264839037
78-79	0.024388435753531057
80-81	0.01819655310108017
82-83	0.024514800705621894
84-85	0.012341643654204836
86-87	0.017606849991322938
88-89	0.022703569725653263
90-91	0.021482041855441864
92-93	0.007750383728237849
94-95	0.011288602386781215
96-97	0.01507955094950625
98-99	0.007076437317086732
100-101	0.010235561119357594
102-103	0.004085800117603649
104-105	0.004043678466906704
106-107	9.266763153327862E-4
108-109	0.01368953647650707
110-111	0.001516379425090014
112-113	0.00252729904181669
114-115	0.013352563270931513
116-117	0.005602179542693663
118-119	0.2759389337156856
120-121	0.0013478928223022346
122-123	6.739464111511173E-4
124-125	0.3106050522392712
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1187038.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.23523144740132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.21896842640034	59.853739711370125
2	8.45833241624872	11.88145869828572
3	2.578992074562307	5.434083157736919
4	1.1977880038517046	3.365076707017811
5	0.6570609623496964	2.307441438284158
6	0.4158788081660628	1.75256066073677
7	0.3059087416271681	1.5039899888977206
8	0.21138814467080422	1.187751621295255
9	0.15631480328318298	0.9880925748524428
>10	0.7614171753190706	9.111847810271723
>50	0.028000521264108607	1.3188435084776038
>100	0.009710166924386743	1.175134314938228
>500	2.3975533258928844E-4	0.11997980783549614
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	8.424330139388966E-5	0.0
12-13	0.0	0.0	0.0	8.424330139388966E-5	8.424330139388966E-5
14-15	4.212165069694483E-5	0.0	0.0	8.424330139388966E-5	8.424330139388966E-5
16-17	8.424330139388966E-5	0.0	0.0	8.424330139388966E-5	8.424330139388966E-5
18-19	8.424330139388966E-5	0.0	0.0	8.424330139388966E-5	1.6848660278777933E-4
20-21	8.424330139388966E-5	0.0	0.0	1.6848660278777933E-4	2.948515548786138E-4
22-23	8.424330139388966E-5	0.0	0.0	3.3697320557555865E-4	3.3697320557555865E-4
24-25	8.424330139388966E-5	0.0	0.0	3.790948562725035E-4	3.3697320557555865E-4
26-27	8.424330139388966E-5	0.0	0.0	5.897031097572276E-4	5.05459808363338E-4
28-29	8.424330139388966E-5	0.0	0.0	0.0011794062195144553	5.05459808363338E-4
30-31	8.424330139388966E-5	0.0	0.0	0.0042964083710883725	5.05459808363338E-4
32-33	8.424330139388966E-5	0.0	0.0	0.011836183845841498	5.05459808363338E-4
34-35	8.424330139388966E-5	0.0	0.0	0.02004990573174574	5.897031097572276E-4
36-37	8.424330139388966E-5	0.0	0.0	0.032180941132465855	5.897031097572276E-4
38-39	8.424330139388966E-5	0.0	0.0	0.05627452533111829	5.897031097572276E-4
40-41	8.424330139388966E-5	0.0	0.0	0.08487512615434384	5.897031097572276E-4
42-43	8.424330139388966E-5	0.0	0.0	0.11221207745666104	6.318247604541725E-4
44-45	8.424330139388966E-5	0.0	0.0	0.14338209897240023	6.739464111511173E-4
46-47	8.424330139388966E-5	0.0	0.0	0.1736254441728066	8.003113632419519E-4
48-49	8.424330139388966E-5	0.0	0.0	0.21006067202566386	8.845546646358414E-4
50-51	8.424330139388966E-5	0.0	0.0	0.2501604834891553	0.001010919616726676
52-53	8.424330139388966E-5	0.0	0.0	0.2957361095432497	0.0010951629181205657
54-55	8.424330139388966E-5	0.0	0.0	0.34367054803637287	0.0011372845688175106
56-57	8.424330139388966E-5	0.0	0.0	0.39531169179082726	0.0011794062195144553
58-59	8.424330139388966E-5	0.0	0.0	0.4591259925966987	0.0011794062195144553
60-61	8.424330139388966E-5	0.0	0.0	0.5246672810811448	0.0011794062195144553
62-63	8.424330139388966E-5	0.0	0.0	0.6021711183635233	0.0011794062195144553
64-65	8.424330139388966E-5	0.0	0.0	0.6710821389037251	0.0011794062195144553
66-67	8.424330139388966E-5	0.0	0.0	0.7538933041739186	0.0011794062195144553
68-69	8.424330139388966E-5	0.0	0.0	0.8406639046096249	0.0011794062195144553
70-71	8.424330139388966E-5	0.0	0.0	0.9335421443963883	0.0012215278702114001
72-73	8.424330139388966E-5	0.0	0.0	1.022376705716245	0.001474257774393069
74-75	8.424330139388966E-5	0.0	0.0	1.1209834899977928	0.0016006227264839035
76-77	8.424330139388966E-5	0.0	0.0	1.2372392459213606	0.0016006227264839035
78-79	8.424330139388966E-5	0.0	0.0	1.3676478764791018	0.0016006227264839035
80-81	8.424330139388966E-5	0.0	0.0	1.4928334223504218	0.0016006227264839035
82-83	8.424330139388966E-5	0.0	0.0	1.632213964506612	0.0016006227264839035
84-85	8.424330139388966E-5	0.0	0.0	1.7801030801035855	0.0016006227264839035
86-87	8.424330139388966E-5	0.0	0.0	1.953728524276392	0.0016006227264839035
88-89	8.424330139388966E-5	0.0	0.0	2.153764243436183	0.0016006227264839035
90-91	8.424330139388966E-5	0.0	0.0	2.3553584636717613	0.0016006227264839035
92-93	8.424330139388966E-5	0.0	0.0	2.5449901351094066	0.0016006227264839035
94-95	8.424330139388966E-5	0.0	0.0	2.7436358397961986	0.0016006227264839035
96-97	8.424330139388966E-5	0.0	0.0	2.9679336297574297	0.0016006227264839035
98-99	8.424330139388966E-5	0.0	0.0	3.231867893024486	0.0016006227264839035
100-101	8.424330139388966E-5	0.0	0.0	3.5110923154945333	0.0016006227264839035
102-103	8.424330139388966E-5	0.0	0.0	3.806280843578723	0.0016006227264839035
104-105	8.424330139388966E-5	0.0	0.0	4.112926460652481	0.0016006227264839035
106-107	8.424330139388966E-5	0.0	0.0	4.434441020422261	0.0016006227264839035
108-109	8.424330139388966E-5	0.0	0.0	4.778954001472573	0.0016006227264839035
110-111	8.424330139388966E-5	0.0	0.0	5.160913129992469	0.0016006227264839035
112-113	8.424330139388966E-5	0.0	0.0	5.539418283155214	0.0016006227264839035
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3000	0.0	35.915066	1
GTACATG	7055	0.0	34.59443	1
TACATGG	7195	0.0	33.33512	2
GGTATCA	2155	0.0	33.147694	1
ACATGGG	7370	0.0	32.05906	3
ATGGGGG	3565	0.0	30.547995	5
TCAACGC	3530	0.0	30.347683	4
CATGGGG	5920	0.0	29.757591	4
ATCAACG	3675	0.0	29.636133	3
CAACGCA	3610	0.0	29.507809	5
TATCAAC	3715	0.0	29.156832	2
GTATAGG	295	0.0	28.250448	1
AACGCAG	3835	0.0	28.238539	6
CTAGCGA	135	8.232106E-5	26.45119	4
TGGGGGG	2900	0.0	24.826963	6
ACGCAGA	4375	0.0	24.611904	7
CGCAGAG	4620	0.0	23.433516	8
ATACCGG	135	0.0025546735	22.038012	6
TATCACG	140	0.0031510536	21.255423	2
GTATAGT	285	8.0061E-8	20.886923	1
>>END_MODULE