FastQCFastQC Report
Mon 15 Aug 2016
SRR2921580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187038
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20990.1768266896257744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16910.14245542265706743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.11111691453854046No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35500.045.6998331
GGTATCA22050.042.4683231
TCAACGC46700.033.953314
CAACGCA48150.033.1616335
TATCAAC48100.032.9748042
ATCAACG49050.032.336153
AACGCAG50500.031.6251356
GTACATG69450.031.1751421
TACATGG71300.029.939132
ACATGGG72000.029.151163
CATGGGG55400.028.9440884
ACGCAGA59950.026.6377777
TAACGCA1358.143663E-526.4932234
GTATAGG4150.025.8701231
ATGGGGG33000.025.4567265
CGCAGAG63100.025.3058628
AGAGTAC64450.023.01930210-11
GCAGAGT69100.022.8498619
TATCACG1350.002523794322.0842132
GTATTAG3807.2759576E-1221.9744741
Produced by FastQC (version 0.11.5)