FastQCFastQC Report
Mon 15 Aug 2016
SRR2921579_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921579_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816430
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38200.036.7770041
GTACATG103850.035.3676641
CGCCTTA8550.034.8054922
TACATGG106200.033.9058572
CATGGGG87450.033.211764
ACATGGG109600.032.5816153
GGTATCA27500.032.037361
ATGGGGG55000.030.8382825
TCAACGC43650.029.8601364
CAACGCA44200.029.3523045
AACGCAG46650.028.3179266
TATCAAC48500.028.1020242
ATCAACG47200.027.7403933
TATACCG1859.24696E-725.7351235
ACGCAGA56100.023.434627
CGTATAC1300.002049458422.8906753
GAGTACA74100.022.0923631
CGCAGAG60050.021.8931248
ATGGGGA29500.021.3841425
AGTACAT75150.021.3042962
Produced by FastQC (version 0.11.5)