##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921579_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1816430
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.764690629421448	31.0	31.0	34.0	26.0	34.0
2	30.097239640393518	31.0	31.0	34.0	26.0	34.0
3	30.15774403637905	31.0	31.0	34.0	26.0	34.0
4	33.731304261656106	37.0	35.0	37.0	30.0	37.0
5	33.613443953248954	37.0	35.0	37.0	28.0	37.0
6	33.69746260522013	37.0	35.0	37.0	29.0	37.0
7	33.57335763007658	37.0	35.0	37.0	28.0	37.0
8	33.65525949252105	37.0	35.0	37.0	29.0	37.0
9	35.29479033048342	39.0	37.0	39.0	29.0	39.0
10-11	35.31351717379695	39.0	37.0	39.0	29.0	39.0
12-13	35.23922391724426	39.0	37.0	39.0	28.5	39.0
14-15	36.51667116266523	40.0	38.0	41.0	28.0	41.0
16-17	36.479527699938885	40.0	38.0	41.0	27.5	41.0
18-19	36.42295326547128	40.0	38.0	41.0	27.0	41.0
20-21	36.464999752261306	40.0	38.0	41.0	28.0	41.0
22-23	36.39233413894287	40.0	38.0	41.0	27.0	41.0
24-25	36.36204037590218	40.0	38.0	41.0	27.0	41.0
26-27	36.31607135975513	40.0	38.0	41.0	27.5	41.0
28-29	36.253631298756346	40.0	37.0	41.0	27.0	41.0
30-31	36.158908132986134	40.0	37.0	41.0	27.0	41.0
32-33	36.06299334408703	40.0	37.0	41.0	27.0	41.0
34-35	35.92418782997418	40.0	37.0	41.0	26.5	41.0
36-37	35.818248707629806	40.0	37.0	41.0	26.0	41.0
38-39	35.6974606783636	40.0	36.0	41.0	25.0	41.0
40-41	35.58599147778885	40.0	36.0	41.0	25.0	41.0
42-43	35.32385751171253	40.0	35.5	41.0	24.0	41.0
44-45	34.949490208816194	39.5	35.0	41.0	23.0	41.0
46-47	34.68247661621973	39.0	35.0	41.0	22.5	41.0
48-49	34.47627764350952	39.0	35.0	41.0	21.5	41.0
50-51	34.26316180640046	39.0	34.0	40.0	20.0	41.0
52-53	34.05025104187885	38.0	34.0	40.0	19.5	41.0
54-55	33.80375241545229	38.0	34.0	40.0	18.0	41.0
56-57	33.54437688212593	38.0	33.5	40.0	17.5	41.0
58-59	33.5467265460271	37.5	34.0	40.0	17.5	41.0
60-61	33.32674008907583	37.0	33.5	40.0	14.5	41.0
62-63	33.011274037535166	36.5	33.0	40.0	13.0	41.0
64-65	32.71392621791095	36.0	33.0	39.0	11.0	41.0
66-67	32.39167955825438	35.5	33.0	39.0	8.0	41.0
68-69	32.06010003138023	35.0	32.5	38.5	7.0	40.0
70-71	31.704103929135723	35.0	32.0	37.5	6.0	40.0
72-73	31.364953507704673	35.0	32.0	37.0	2.0	39.0
74-75	31.008805183794586	35.0	32.0	36.5	2.0	39.0
76-77	30.721554367633214	35.0	31.0	36.0	2.0	38.5
78-79	30.45739940432607	35.0	31.0	35.5	2.0	37.0
80-81	30.212466211194485	35.0	31.0	35.0	2.0	37.0
82-83	29.995237911727948	35.0	31.0	35.0	2.0	36.5
84-85	29.793677983737332	35.0	31.0	35.0	2.0	36.0
86-87	29.630421761367078	34.5	31.0	35.0	2.0	36.0
88-89	29.47638554747499	34.0	31.0	35.0	2.0	35.0
90-91	29.294687381291872	34.0	30.0	35.0	2.0	35.0
92-93	29.217990784120502	34.0	30.0	35.0	2.0	35.0
94-95	29.092780894391744	34.0	30.0	35.0	2.0	35.0
96-97	29.009071640525647	34.0	30.0	35.0	2.0	35.0
98-99	28.92289573504071	34.0	30.0	35.0	2.0	35.0
100-101	28.816932114091927	34.0	29.5	35.0	2.0	35.0
102-103	27.91923388184516	33.0	28.0	34.5	2.0	35.0
104-105	28.87015464399949	34.0	29.5	35.0	2.0	35.0
106-107	29.162565581938196	34.0	31.0	35.0	2.0	35.0
108-109	29.174408592679043	35.0	31.0	35.0	2.0	35.0
110-111	29.119212686423367	35.0	31.0	35.0	2.0	35.0
112-113	29.041520730223567	35.0	31.0	35.0	2.0	35.0
114-115	28.922049569760464	34.0	30.5	35.0	2.0	35.0
116-117	28.80454930825851	34.0	30.0	35.0	2.0	35.0
118-119	28.517737540119906	34.0	30.0	35.0	2.0	35.0
120-121	28.391639094267326	34.0	30.0	35.0	2.0	35.0
122-123	28.13252946714159	34.0	29.5	35.0	2.0	35.0
124-125	27.32489195840192	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78613.0
3	14199.0
4	6983.0
5	6031.0
6	6262.0
7	6907.0
8	6754.0
9	7291.0
10	7689.0
11	7795.0
12	7817.0
13	7549.0
14	7376.0
15	6886.0
16	6154.0
17	5654.0
18	5392.0
19	5093.0
20	5144.0
21	5328.0
22	6174.0
23	7892.0
24	10941.0
25	13548.0
26	15922.0
27	18120.0
28	21381.0
29	26055.0
30	31733.0
31	40019.0
32	52362.0
33	73255.0
34	114871.0
35	189171.0
36	361335.0
37	503726.0
38	118965.0
39	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.43167264234946	17.117564102069913	12.028275781937046	25.422487473643578
2	16.6911956199163	18.958926254018646	38.33619366806132	26.013684458003734
3	20.72450737539787	22.90191665092903	27.667803997377	28.705771976296095
4	13.9462013032598	14.601355015788329	33.91349592421602	37.538947756735844
5	15.067615152798297	37.17718369219096	32.09994895618987	15.655252198820872
6	35.1124189921968	32.88492483357581	16.957964114094256	15.044692060133139
7	30.687013985752028	31.166279948613873	19.553830932596476	18.59287513303762
8	27.897300197304713	35.12773029073527	18.50687371727149	18.468095794688526
9	28.132122778739337	15.958163286097326	18.008862739871923	37.900851195291416
10-11	27.098894894481358	25.152311770578716	25.76657141873515	21.98222191620477
12-13	26.790539433754645	22.41897399035289	26.2197295824807	24.570756993411763
14-15	24.42914497510731	23.824401692939762	23.297939390201357	28.448513941751575
16-17	25.064050385569004	26.869962847471967	24.002482709700303	24.06350405725873
18-19	25.076364761944625	26.224755291282136	25.201032370066994	23.497847576706242
20-21	25.506069864570758	25.15499577058974	25.2334986915262	24.105435673313302
22-23	25.809383918726752	24.590874493721987	24.90147061577285	24.698270971778406
24-25	25.25749052712968	25.073994537524847	24.811719732454918	24.85679520289056
26-27	24.77731437724035	25.335598194280124	25.15237689224946	24.734710536230065
28-29	24.898565053171932	25.63180484392664	24.938084268185378	24.53154583471605
30-31	25.533861035881493	25.168289166605174	24.881079693951612	24.416770103561717
32-33	24.999903627196957	25.234888056105493	24.966558637343635	24.798649679353918
34-35	25.04337499903611	25.626362525763373	25.018671904346974	24.311590570853543
36-37	25.180143685494105	25.50809810387673	24.930895024028793	24.380863186600365
38-39	25.228266720263655	25.577987056878428	24.908722221227986	24.285024001629935
40-41	25.104378743720822	25.38888156052177	24.900674616736342	24.606065079021064
42-43	25.073470581206397	25.237123994993482	24.807451186184394	24.881954237615727
44-45	24.774478877303185	25.30065353359099	25.172716445490874	24.752151143614956
46-47	24.807673828690184	25.272076037842712	25.117559811023238	24.80269032244387
48-49	24.795272847924643	25.523840205298498	25.091628496730944	24.589258450045914
50-51	24.83338913645405	25.476042839174674	25.090698427453372	24.599869596917905
52-53	24.950488288859294	25.34939037199932	25.074828557182066	24.625292781959317
54-55	24.613429807244906	25.102297037226705	25.240482699947965	25.043790455580428
56-57	24.645589639499242	25.331557610406318	24.95517556969428	25.06767718040016
58-59	24.768404823522932	25.424315841638677	25.077336049776992	24.729943285061395
60-61	25.128950068088717	25.316421193020094	25.206757881138515	24.34787085775267
62-63	24.852624753755105	25.286070833178464	25.27472739261933	24.586577020447102
64-65	24.64657877906104	25.103141702451364	25.234661999444402	25.015617519043186
66-67	24.788709668981465	25.322569675903555	25.0216487405458	24.867071914569184
68-69	24.62555354749468	25.33928703192817	25.16148003264292	24.87367938793423
70-71	24.60297373464531	25.19952179213931	25.292525014066154	24.904979459149224
72-73	24.756131070016746	25.53064722800251	24.96207401060661	24.75114769137413
74-75	24.570132184009303	25.485972241798514	25.117053983579407	24.826841590612776
76-77	24.627645984798598	25.051262342055825	25.19199977094378	25.129091902201807
78-79	24.533902061750645	25.22873312000511	25.245776249861645	24.9915885683826
80-81	24.724895179481308	25.449284874761886	24.99944248066096	24.82637746509585
82-83	24.77006728099535	25.382441080945352	25.149507204430037	24.697984433629262
84-85	24.727794287885192	25.424898587869638	24.90541520817188	24.94189191607329
86-87	24.396978290860567	25.63901869062391	25.016456746219767	24.94754627229576
88-89	24.427964526724725	25.278535694914417	25.174626861562267	25.118872916798594
90-91	24.713382775682117	25.146381895477255	25.171271016370927	24.968964312469698
92-93	24.820803182735883	25.181756968428655	25.226298092990195	24.771141755845267
94-95	24.705550702705796	25.1475377973984	25.15092393334653	24.99598756654927
96-97	24.488537942016315	25.052996256950085	25.265834730966226	25.192631070067385
98-99	24.351527300585715	25.0908771583067	25.18661990926186	25.37097563184572
100-101	24.298650169812728	25.328097681754123	25.26023866004172	25.113013488391427
102-103	24.25471986892495	25.175185150113084	25.39058800989229	25.179506971069678
104-105	24.23857787673056	25.238850675900547	25.292942539575836	25.229628907793057
106-107	24.24526533424465	25.15224344196389	25.34093915134552	25.26155207244594
108-109	24.26431438147347	25.09386547352853	25.39053488040446	25.25128526459354
110-111	24.095475166805723	25.471734209714274	25.346046692664842	25.08674393081516
112-113	24.039414399534902	25.58060154416007	25.364128448044255	25.01585560826077
114-115	24.010688326697636	25.697216200754657	25.22716548461144	25.064929987936264
116-117	23.916740005764357	25.454177080267982	25.307728284630393	25.32135462933727
118-119	23.952492511740648	25.331475035961038	25.361508124806253	25.35452432749206
120-121	23.86652385785293	25.43563156977661	25.53084662118696	25.1669979511835
122-123	23.894166081263503	25.457467799155697	25.418930286741116	25.229435832839687
124-125	23.743519195551855	25.43344349136151	25.410248452007256	25.412788861079388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	786.0
1	647.0
2	419.5
3	294.5
4	214.5
5	170.5
6	178.0
7	193.5
8	206.0
9	218.0
10	234.5
11	241.5
12	252.0
13	302.5
14	340.5
15	360.0
16	393.0
17	408.5
18	459.0
19	539.0
20	605.5
21	698.0
22	786.5
23	918.5
24	1133.5
25	1397.0
26	1800.5
27	2492.0
28	3370.5
29	4564.0
30	6365.0
31	8620.5
32	11719.5
33	14744.5
34	17621.0
35	21010.0
36	25113.5
37	29104.0
38	32183.0
39	35470.5
40	38619.5
41	41805.0
42	45038.5
43	49779.5
44	55052.5
45	59758.5
46	64450.0
47	69028.0
48	74443.0
49	78506.5
50	81305.5
51	82679.5
52	81723.5
53	81263.5
54	81530.0
55	78851.0
56	73891.0
57	68590.5
58	63462.5
59	57278.0
60	49317.0
61	42363.5
62	35913.0
63	28497.0
64	21819.5
65	17279.5
66	13819.5
67	11012.5
68	8944.5
69	7237.5
70	5879.5
71	4673.0
72	3628.5
73	3067.0
74	2551.0
75	1992.5
76	1461.5
77	1043.5
78	760.0
79	514.5
80	352.0
81	221.5
82	154.5
83	128.5
84	99.0
85	75.0
86	63.0
87	52.0
88	48.5
89	41.0
90	31.5
91	26.5
92	22.0
93	18.0
94	18.0
95	17.5
96	14.0
97	12.0
98	8.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02526934701584977
2	0.01150608611397081
3	0.010405025241820494
4	0.011836404375615906
5	0.018883193957377933
6	0.09854494805745335
7	0.06353121232307328
8	0.053071134037645275
9	0.028242211370655627
10-11	0.054640145780459476
12-13	0.013130150900392528
14-15	0.029480904851824734
16-17	0.036995645304250646
18-19	0.044152540973227704
20-21	0.031600447030714095
22-23	0.04007861574627153
24-25	0.03228861007580804
26-27	0.04767593576410872
28-29	0.04720798489344483
30-31	0.027966946152618046
32-33	0.03110496963824645
34-35	0.04778604185132375
36-37	0.009441596978688967
38-39	0.022434115270062706
40-41	0.04440027966946153
42-43	0.02185605831218379
44-45	0.0166810722130773
46-47	0.024195812665503212
48-49	0.029591010939039764
50-51	0.028737688763123267
52-53	0.02452613092714831
54-55	0.021745952224968758
56-57	0.025104187885027225
58-59	0.01816750439048023
60-61	0.022957119184334105
62-63	0.022158850052025128
64-65	0.01984662222050946
66-67	0.027774260499991744
68-69	0.02290206614072659
70-71	0.04963031881217553
72-73	0.021635846137753725
74-75	0.009579229587707757
76-77	0.015524958297319466
78-79	0.02471881657977461
80-81	0.019241038740826784
82-83	0.02554461223388735
84-85	0.011148241330521958
86-87	0.01681870482209609
88-89	0.022847013097119076
90-91	0.0206724178746222
92-93	0.007211948712584576
94-95	0.011038135243306926
96-97	0.01453400351238418
98-99	0.005753043056985405
100-101	0.009056225673436356
102-103	0.003633500878096045
104-105	0.004156504792367446
106-107	7.432160887014638E-4
108-109	0.01469916264320673
110-111	0.0011285873939540748
112-113	0.0020920156570856017
114-115	0.013377889596626351
116-117	0.0052575656645177625
118-119	0.28046773065848946
120-121	8.533221759164956E-4
122-123	9.90954784935285E-4
124-125	0.31369224247562527
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1816430.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.20266697575174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.52440209764097	49.283081650034454
2	10.553352261861079	12.9178660792096
3	3.505286994688147	6.435987377709969
4	1.6333982479065343	3.9987331602159997
5	0.9462928316936556	2.8957822519843948
6	0.5936919100732633	2.180131695504713
7	0.4202871339022898	1.8005885443290515
8	0.2885689686859047	1.4128952392015646
9	0.2233405999211687	1.2302136323225905
>10	1.23311810617083	13.40336945685337
>50	0.060028596098816535	2.475457888274975
>100	0.017783322516150366	1.7487255471135257
>500	4.489288412051432E-4	0.21716747724575564
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.505304360751584E-5
2	0.0	0.0	0.0	0.0	5.505304360751584E-5
3	0.0	0.0	0.0	0.0	5.505304360751584E-5
4	0.0	0.0	0.0	0.0	5.505304360751584E-5
5	0.0	0.0	0.0	0.0	5.505304360751584E-5
6	0.0	0.0	0.0	0.0	1.6515913082254752E-4
7	0.0	0.0	0.0	0.0	1.6515913082254752E-4
8	0.0	0.0	0.0	0.0	1.6515913082254752E-4
9	0.0	5.505304360751584E-5	0.0	0.0	1.6515913082254752E-4
10-11	0.0	5.505304360751584E-5	0.0	0.0	1.6515913082254752E-4
12-13	0.0	1.1010608721503168E-4	0.0	0.0	5.230039142714005E-4
14-15	0.0	1.1010608721503168E-4	0.0	0.0	6.331100014864322E-4
16-17	0.0	1.1010608721503168E-4	0.0	0.0	7.707426105052218E-4
18-19	0.0	1.1010608721503168E-4	0.0	2.752652180375792E-5	7.707426105052218E-4
20-21	0.0	1.1010608721503168E-4	0.0	2.477386962338213E-4	9.359017413277693E-4
22-23	0.0	1.1010608721503168E-4	0.0	4.679508706638847E-4	9.909547849352852E-4
24-25	0.0	1.1010608721503168E-4	0.0	6.606365232901901E-4	9.909547849352852E-4
26-27	0.0	1.1010608721503168E-4	0.0	9.634282631315273E-4	0.001046007828542801
28-29	0.0	1.1010608721503168E-4	0.0	0.0030554439202171296	0.001046007828542801
30-31	0.0	1.1010608721503168E-4	0.0	0.011340926983148264	0.001046007828542801
32-33	0.0	1.1010608721503168E-4	0.0	0.03030670050593747	0.001046007828542801
34-35	0.0	1.1010608721503168E-4	0.0	0.052658236210588905	0.001046007828542801
36-37	0.0	1.1010608721503168E-4	0.0	0.08621306628936981	0.001046007828542801
38-39	0.0	1.376326090187896E-4	0.0	0.1358158585797416	0.001073534350346559
40-41	0.0	1.6515913082254752E-4	0.0	0.1941445582819046	0.0011010608721503169
42-43	0.0	1.6515913082254752E-4	0.0	0.25426248190131195	0.0011010608721503169
44-45	0.0	1.6515913082254752E-4	0.0	0.32029860770852714	0.0011561139157578327
46-47	0.0	1.6515913082254752E-4	0.0	0.3937393678809533	0.0012111669593653485
48-49	0.0	1.6515913082254752E-4	0.0	0.4695474089285026	0.0012662200029728643
50-51	0.0	1.6515913082254752E-4	0.0	0.5486586325925029	0.0012662200029728643
52-53	0.0	1.6515913082254752E-4	0.0	0.6314033571345992	0.001348799568384138
54-55	0.0	1.9268565262630543E-4	0.0	0.7275810243169294	0.001376326090187896
56-57	0.0	2.2021217443006337E-4	0.0	0.8326772845636772	0.0014313791337954118
58-59	0.0	2.2021217443006337E-4	0.0	0.94718761526731	0.0015139586992066857
60-61	0.0	2.2021217443006337E-4	0.0	1.0603766729243627	0.0015414852210104437
62-63	0.0	2.2021217443006337E-4	0.0	1.177694708851979	0.0015414852210104437
64-65	0.0	2.2021217443006337E-4	0.0	1.3036560726259752	0.0015414852210104437
66-67	0.0	3.3031826164509504E-4	0.0	1.4533728247166144	0.0015414852210104437
68-69	0.0	3.3031826164509504E-4	0.0	1.6153113524881224	0.0015414852210104437
70-71	0.0	3.3031826164509504E-4	0.0	1.7816265972264276	0.0015965382646179595
72-73	0.0	3.3031826164509504E-4	0.0	1.9456571406550212	0.001734170873636749
74-75	0.0	3.3031826164509504E-4	0.0	2.1236986836817273	0.0018167504390480228
76-77	0.0	3.3031826164509504E-4	0.0	2.333368200260951	0.0018718034826555386
78-79	0.0	3.3031826164509504E-4	0.0	2.570288973425896	0.0019268565262630544
80-81	0.0	3.3031826164509504E-4	0.0	2.8049525718029322	0.0019268565262630544
82-83	0.0	3.3031826164509504E-4	0.0	3.04812186541733	0.0020369626134780863
84-85	0.0	3.3031826164509504E-4	0.0	3.301393392533706	0.0020369626134780863
86-87	0.0	3.3031826164509504E-4	0.0	3.58365034710944	0.0022021217443006337
88-89	0.0	3.3031826164509504E-4	0.0	3.902958000033032	0.0023397543533194235
90-91	0.0	3.3031826164509504E-4	0.0	4.24084605517416	0.002394807396926939
92-93	0.0	3.3031826164509504E-4	0.0	4.580027856840065	0.0025874930495532445
94-95	0.0	3.3031826164509504E-4	0.0	4.930715744619941	0.002697599136768276
96-97	0.0	3.853713052526109E-4	0.0	5.32200525206036	0.00291781131119834
98-99	0.0	3.853713052526109E-4	0.0	5.758025357431886	0.0030279173984133715
100-101	0.0	3.853713052526109E-4	0.0	6.225205485485265	0.0030279173984133715
102-103	0.0	3.853713052526109E-4	0.0	6.702267634866194	0.0030279173984133715
104-105	0.0	3.853713052526109E-4	0.0	7.19777255385564	0.0030279173984133715
106-107	0.0	3.853713052526109E-4	0.0	7.731924709457562	0.0030554439202171296
108-109	0.0	3.853713052526109E-4	0.0	8.302632086014876	0.0030829704420208873
110-111	0.0	3.853713052526109E-4	0.0	8.91129853613957	0.0030829704420208873
112-113	0.0	3.853713052526109E-4	0.0	9.51743254625832	0.003138023485628403
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3820	0.0	36.777004	1
GTACATG	10385	0.0	35.367664	1
CGCCTTA	855	0.0	34.805492	2
TACATGG	10620	0.0	33.905857	2
CATGGGG	8745	0.0	33.21176	4
ACATGGG	10960	0.0	32.581615	3
GGTATCA	2750	0.0	32.03736	1
ATGGGGG	5500	0.0	30.838282	5
TCAACGC	4365	0.0	29.860136	4
CAACGCA	4420	0.0	29.352304	5
AACGCAG	4665	0.0	28.317926	6
TATCAAC	4850	0.0	28.102024	2
ATCAACG	4720	0.0	27.740393	3
TATACCG	185	9.24696E-7	25.735123	5
ACGCAGA	5610	0.0	23.43462	7
CGTATAC	130	0.0020494584	22.890675	3
GAGTACA	7410	0.0	22.092363	1
CGCAGAG	6005	0.0	21.893124	8
ATGGGGA	2950	0.0	21.384142	5
AGTACAT	7515	0.0	21.304296	2
>>END_MODULE