FastQCFastQC Report
Mon 15 Aug 2016
SRR2921579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816430
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26910.14814774034782513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.11687761157875612No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31200.047.233431
GTATCAA50700.046.3655471
TCAACGC63450.036.752294
CAACGCA64700.036.0084465
ATCAACG65050.035.945953
GTACATG106150.035.8597951
TACATGG108400.034.7775542
AACGCAG67500.034.612586
TATCAAC68300.034.5848352
ACATGGG109600.033.6892473
CATGGGG91100.032.4715164
ATGGGGG56100.030.2701055
ACGCAGA79200.029.4208587
CGCAGAG84100.027.9862678
GCAGAGT93850.024.8883279
CGTATAC1552.0712656E-423.0903643
GTATAGG4400.023.0517221
AGAGTAC85700.021.97101810-11
GAGTACT34100.021.73820512-13
ATGGGGA31600.021.6841645
Produced by FastQC (version 0.11.5)