##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921579_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1816430 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72761350561266 33.0 31.0 34.0 30.0 34.0 2 31.907636958209235 33.0 31.0 34.0 30.0 34.0 3 31.978076776974614 34.0 31.0 34.0 30.0 34.0 4 35.37423627665255 37.0 35.0 37.0 33.0 37.0 5 35.23900728351767 37.0 35.0 37.0 33.0 37.0 6 35.26662629443469 37.0 35.0 37.0 33.0 37.0 7 35.16323062270498 37.0 35.0 37.0 33.0 37.0 8 35.199862367390985 37.0 36.0 37.0 33.0 37.0 9 36.92479534031039 39.0 38.0 39.0 34.0 39.0 10-11 36.87657437941456 39.0 38.0 39.0 34.0 39.0 12-13 36.81030510396768 39.0 38.0 39.0 33.0 39.0 14-15 38.22393073226053 41.0 39.0 41.0 34.0 41.0 16-17 38.22180926322512 41.0 39.0 41.0 34.0 41.0 18-19 38.23049277979333 41.0 39.0 41.0 34.0 41.0 20-21 38.162158464680715 41.0 39.0 41.0 34.0 41.0 22-23 38.1674551180062 41.0 39.0 41.0 34.0 41.0 24-25 38.11953915097196 41.0 39.0 41.0 34.0 41.0 26-27 38.04460370066559 41.0 39.0 41.0 33.5 41.0 28-29 37.97258248322258 41.0 38.5 41.0 33.0 41.0 30-31 37.86044383763756 41.0 38.0 41.0 33.0 41.0 32-33 37.740899456626465 41.0 38.0 41.0 33.0 41.0 34-35 37.60952169915714 41.0 38.0 41.0 33.0 41.0 36-37 37.5321435453059 40.0 38.0 41.0 33.0 41.0 38-39 37.45035041262256 40.0 38.0 41.0 32.0 41.0 40-41 37.33001794729222 40.0 38.0 41.0 32.0 41.0 42-43 37.165522480910354 40.0 37.5 41.0 31.5 41.0 44-45 37.01634579917751 40.0 37.0 41.0 31.0 41.0 46-47 36.821985157699444 40.0 37.0 41.0 31.0 41.0 48-49 36.635763282923094 40.0 36.5 41.0 30.5 41.0 50-51 36.39326563644071 40.0 36.0 41.0 30.0 41.0 52-53 36.261011985047595 40.0 35.0 41.0 30.0 41.0 54-55 36.12750670270806 39.5 35.0 41.0 30.0 41.0 56-57 35.92716482330726 39.0 35.0 41.0 29.0 41.0 58-59 35.729582202452065 39.0 35.0 41.0 29.0 41.0 60-61 35.46757017886734 38.5 35.0 41.0 29.0 41.0 62-63 35.10949444790055 37.5 35.0 40.5 28.5 41.0 64-65 34.82362986737722 37.0 35.0 40.0 28.0 41.0 66-67 34.52208590476924 36.5 35.0 39.5 28.0 41.0 68-69 34.154712265267584 36.0 35.0 39.0 28.0 41.0 70-71 33.78886221874777 35.5 34.5 39.0 28.0 41.0 72-73 33.477910792048135 35.0 34.0 37.0 27.5 39.5 74-75 33.16017077454127 35.0 34.0 37.0 27.0 39.0 76-77 32.841686439884825 35.0 34.0 36.5 26.0 39.0 78-79 32.59007200938104 35.0 34.0 36.0 26.0 38.0 80-81 32.349194849237236 35.0 34.0 36.0 26.0 37.0 82-83 32.13162687249165 35.0 34.0 35.0 26.0 37.0 84-85 31.927301079590187 35.0 34.0 35.0 25.5 36.0 86-87 31.809068337343028 35.0 34.0 35.0 25.0 36.0 88-89 31.71827540835595 35.0 34.0 35.0 25.0 36.0 90-91 31.616466915873445 35.0 33.5 35.0 25.0 35.5 92-93 31.53807275810243 35.0 33.0 35.0 25.0 35.0 94-95 31.47194909795588 35.0 33.0 35.0 24.0 35.0 96-97 31.404542977158492 35.0 33.0 35.0 24.0 35.0 98-99 31.33122526053853 35.0 33.0 35.0 24.0 35.0 100-101 31.264371597033744 35.0 33.0 35.0 23.5 35.0 102-103 30.56945106610219 34.0 31.5 35.0 21.5 35.0 104-105 31.08962718078869 35.0 33.0 35.0 23.0 35.0 106-107 31.189489823444887 35.0 33.0 35.0 23.0 35.0 108-109 31.17477386962338 35.0 33.0 35.0 23.0 35.0 110-111 31.108125278706034 35.0 33.0 35.0 23.0 35.0 112-113 31.07051056192642 35.0 33.0 35.0 22.5 35.0 114-115 30.942179715155554 35.0 33.0 35.0 20.0 35.0 116-117 31.133045314160192 35.0 33.0 35.0 23.0 35.0 118-119 31.11566616935417 35.0 33.0 35.0 21.5 35.0 120-121 31.033687232648656 35.0 33.0 35.0 19.0 35.0 122-123 30.894708301448446 35.0 33.0 35.0 16.5 35.0 124-125 30.248934723606194 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29570.0 3 7866.0 4 4323.0 5 3427.0 6 3491.0 7 3787.0 8 4319.0 9 4654.0 10 4807.0 11 5176.0 12 5684.0 13 7037.0 14 9104.0 15 8245.0 16 5030.0 17 4578.0 18 4210.0 19 3846.0 20 3744.0 21 3912.0 22 4256.0 23 5174.0 24 6835.0 25 8708.0 26 10758.0 27 12832.0 28 15555.0 29 18897.0 30 22897.0 31 28826.0 32 37474.0 33 50889.0 34 80615.0 35 147168.0 36 334330.0 37 673578.0 38 230585.0 39 243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.023044585832935 17.5165394794373 11.19018171125103 27.270234223478734 2 16.0395884797678 19.271223588365256 37.3843789897197 27.30480894214724 3 19.185224266268968 22.968430235247727 28.134121384214456 29.71222411426885 4 13.64691388933158 14.622374888262318 33.67306367765726 38.05764754474884 5 14.945108681286731 37.12812893732874 32.35333312774787 15.57342925363666 6 35.42410540889415 33.193212726770874 16.18855789451067 15.194123969824302 7 31.055923886579016 31.4087945550172 18.716191949304726 18.819089609099063 8 28.694476344699005 34.31766043992058 18.148059408830907 18.83980380654951 9 28.408704775069737 15.158567781978594 18.094653276681985 38.338074166269685 10-11 27.44617844140798 24.69526664461258 25.60952868623861 22.249026227740824 12-13 27.204123885113574 21.87274753900777 26.399827473709987 24.52330110216867 14-15 24.56196742750321 23.298846006276083 23.782125808645326 28.357060757575383 16-17 24.8100754740677 26.474256083038 24.43887437770524 24.27679406518906 18-19 24.861405300138898 25.600778972157695 26.16978002187555 23.36803570582786 20-21 25.432895128831902 24.570821890878214 26.176849740441888 23.819433239847992 22-23 25.350380013047896 24.038984025963657 25.93260789975693 24.67802806123151 24-25 24.817553009484133 24.52151967448996 25.92725133571485 24.733675980311055 26-27 24.48008998156765 24.765273853593868 26.187421959818803 24.567214205019678 28-29 24.52352192421276 25.190739880243214 25.939990574493432 24.34574762105059 30-31 25.196646866408955 24.672062532808887 25.82910998488486 24.3021806158973 32-33 24.721874132835314 24.729995198935836 25.91894680509001 24.62918386313884 34-35 24.862127419204768 24.90347405319787 26.07433805470418 24.16006047289318 36-37 24.88846874454594 24.825759081476864 26.02115171058637 24.264620463390827 38-39 24.903008324740295 25.00367486551644 25.97909703461751 24.114219775125758 40-41 24.903633637427944 24.766162062855397 25.85982358823933 24.470380711477336 42-43 24.81724538502618 24.670441595049468 25.714144144640134 24.798168875284222 44-45 24.61098397854373 24.756606053198578 25.873078662622284 24.75933130563541 46-47 24.678537913545824 24.77631645231273 25.7428154658561 24.802330168285344 48-49 24.69244075362338 25.061935765179218 25.64432464691108 24.601298834286318 50-51 24.760144615166094 25.12644467897338 25.541109453993517 24.57230125186701 52-53 24.898295683674046 25.0132472297806 25.438397284765202 24.650059801780152 54-55 24.47083203493615 24.7918068293306 25.697445745349718 25.039915390383527 56-57 24.584725381292913 24.955922877905063 25.414214840908084 25.04513689989394 58-59 24.60259020704755 25.01210486460031 25.620802694973655 24.764502233378487 60-61 24.970711748552652 24.89740854027795 25.74062941112998 24.39125030003942 62-63 24.70054181183662 24.92139007506859 25.769796446883692 24.608271666211095 64-65 24.4784549913842 24.643999493512 25.808646631029 25.06889888407481 66-67 24.572490818885747 24.99220283153519 25.554617479035734 24.88068887054333 68-69 24.466815072179084 24.94820166817192 25.674107770809552 24.910875488839444 70-71 24.403637905121585 24.79834070126567 25.79981061752999 24.998210776082754 72-73 24.615038195445173 25.152810684319345 25.414264569420027 24.817886550815455 74-75 24.3503337624939 25.20251616371746 25.495771295682268 24.95137877810637 76-77 24.373531601787608 24.76438875408261 25.55314999373759 25.30892965039219 78-79 24.32067222042382 24.857467513163197 25.657526750303344 25.16433351610964 80-81 24.440385811729602 25.18883193957378 25.347742549946872 25.023039698749745 82-83 24.524237586792864 25.148438753821946 25.40968105040875 24.91764260897644 84-85 24.419230408204783 25.12559840057652 25.2390367894376 25.216134401781098 86-87 24.101947226152397 25.360349696932992 25.383884873075207 25.1538182038394 88-89 23.98866660262062 25.038874940369737 25.521284804048005 25.45117365296164 90-91 24.258313819199707 24.85919406788689 25.581026619140907 25.301465493772497 92-93 24.35590691631387 24.979437688212595 25.535556008213916 25.129099387259622 94-95 24.251565824357353 24.89421928450367 25.482864088400227 25.37135080273875 96-97 24.04663594673009 24.81568038053083 25.514834205933685 25.622849466805402 98-99 23.863647937379966 24.823175094024467 25.506416345819556 25.80676062277601 100-101 23.8404727955385 25.02257174787908 25.597600788359586 25.53935466822283 102-103 23.86980237920115 24.848024232441173 25.6573413021744 25.62483208618328 104-105 23.94482583969655 24.894711054100625 25.498119938560805 25.66234316764202 106-107 23.952561619163415 24.825405144571505 25.528491628723433 25.693541607541647 108-109 23.93515917454793 24.73471367465019 25.564960981597252 25.76516616920463 110-111 23.72113085620192 25.130599654597013 25.536450915505092 25.61181857369597 112-113 23.631295742298338 25.223763588497413 25.504534729187977 25.640405940016276 114-115 23.56622605880766 25.34039296862527 25.364671360856185 25.728709611710883 116-117 23.451729253187132 25.112894670270485 25.466064708568652 25.969311367973724 118-119 23.46743698433186 24.965845781764802 25.498384573606987 26.06833266029635 120-121 23.325851443470714 25.048668069452194 25.665166985431508 25.960313501645576 122-123 23.20191388455418 25.248505225002827 25.480969197216158 26.068611693226835 124-125 23.034310083705716 25.199536829852757 25.453525361927458 26.31262772451407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28.0 1 18.5 2 18.5 3 48.5 4 57.0 5 76.0 6 186.0 7 471.0 8 846.0 9 957.0 10 761.5 11 563.0 12 499.0 13 524.0 14 570.0 15 601.5 16 673.0 17 707.0 18 746.0 19 834.0 20 854.5 21 896.0 22 973.5 23 1089.5 24 1266.5 25 1547.0 26 1934.0 27 2530.0 28 3399.5 29 4605.5 30 6253.0 31 8392.0 32 11542.0 33 14444.5 34 17183.0 35 20674.5 36 24717.0 37 28749.5 38 31905.5 39 35149.0 40 38548.0 41 41311.5 42 44306.5 43 49317.0 44 54600.0 45 59585.5 46 64733.5 47 69505.0 48 74739.5 49 79228.0 50 82059.0 51 83107.5 52 82457.5 53 82182.5 54 82429.0 55 80003.0 56 74979.0 57 69340.5 58 63878.0 59 57444.5 60 49398.5 61 42205.0 62 35664.0 63 27964.0 64 21184.5 65 16624.5 66 13080.0 67 10493.0 68 8466.0 69 6698.5 70 5380.5 71 4235.5 72 3342.0 73 2820.0 74 2328.0 75 1826.5 76 1348.5 77 911.0 78 566.0 79 350.0 80 209.0 81 108.0 82 76.5 83 52.5 84 24.5 85 12.5 86 10.0 87 5.0 88 3.0 89 2.0 90 1.0 91 1.5 92 2.0 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025985036582747475 2 0.002367280875123181 3 0.03534405399602517 4 0.1668657751743805 5 0.026315354844392572 6 0.07305538886717353 7 0.056924847090171385 8 0.04817141315657636 9 0.03550921312684772 10-11 0.06581591363278519 12-13 0.058053434484125456 14-15 0.061962200580259086 16-17 0.053511558386505405 18-19 0.03875734269969115 20-21 0.02526934701584977 22-23 0.002477386962338213 24-25 0.004431770010405025 26-27 0.003193076529235919 28-29 0.004514349575816299 30-31 0.0021745952224968756 32-33 0.00913880523884763 34-35 0.004349190444993751 36-37 0.006771524363724449 38-39 0.0020369626134780863 40-41 0.0030003908766096134 42-43 0.0033031826164509506 44-45 0.004651982184835089 46-47 0.004431770010405025 48-49 0.001761697395440507 50-51 0.0012662200029728643 52-53 6.88163045093948E-4 54-55 0.004844667837461394 56-57 6.331100014864322E-4 58-59 6.331100014864322E-4 60-61 1.1010608721503168E-4 62-63 0.001348799568384138 64-65 0.0 66-67 0.003605974356292288 68-69 5.780569578789163E-4 70-71 0.0 72-73 0.0026700726149645184 74-75 0.005147459577302731 76-77 0.002064489135281844 78-79 1.1010608721503168E-4 80-81 0.0 82-83 0.005973255231415469 84-85 0.001403852611991654 86-87 0.0 88-89 0.0015690117428142016 90-91 6.331100014864322E-4 92-93 0.0 94-95 0.0030003908766096134 96-97 0.0011010608721503169 98-99 7.982691323089797E-4 100-101 0.0 102-103 0.0012111669593653485 104-105 0.0 106-107 5.780569578789163E-4 108-109 0.0023948073969269393 110-111 5.505304360751584E-5 112-113 2.2021217443006337E-4 114-115 0.0 116-117 0.001348799568384138 118-119 0.0020094360916743286 120-121 0.002422333918730697 122-123 0.001073534350346559 124-125 0.10889492025566634 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1816430.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.736676754691786 #Duplication Level Percentage of deduplicated Percentage of total 1 77.74196897818292 44.885629331666244 2 12.092780649158383 13.963939348116991 3 3.954184360089729 6.849043926808754 4 1.886351654742477 4.356467029421774 5 1.055882013668713 3.048155925714177 6 0.6694882317540158 2.3192415376711075 7 0.49579223920778476 2.0037777376837282 8 0.3473451014608513 1.6043641476296635 9 0.2675118070774702 1.390071845996583 >10 1.396827869217197 14.284252993392846 >50 0.06828051229655102 2.6483467425398466 >100 0.022819743854317374 2.097536475936621 >500 4.7927455599060514E-4 0.2011180618029281 >1k 2.8756473359436307E-4 0.34805489561877123 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2691 0.14814774034782513 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2123 0.11687761157875612 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.505304360751584E-5 2 0.0 0.0 0.0 0.0 5.505304360751584E-5 3 0.0 0.0 0.0 0.0 5.505304360751584E-5 4 0.0 0.0 0.0 0.0 5.505304360751584E-5 5 0.0 0.0 0.0 0.0 5.505304360751584E-5 6 0.0 0.0 0.0 0.0 5.505304360751584E-5 7 0.0 0.0 0.0 0.0 1.1010608721503168E-4 8 0.0 0.0 0.0 0.0 1.1010608721503168E-4 9 0.0 0.0 0.0 0.0 1.1010608721503168E-4 10-11 0.0 0.0 0.0 0.0 1.6515913082254752E-4 12-13 0.0 0.0 0.0 0.0 3.853713052526109E-4 14-15 0.0 0.0 0.0 0.0 5.230039142714005E-4 16-17 0.0 0.0 0.0 0.0 6.055834796826743E-4 18-19 0.0 0.0 0.0 2.752652180375792E-5 7.156895668977059E-4 20-21 0.0 0.0 0.0 1.1010608721503168E-4 8.808486977202535E-4 22-23 0.0 0.0 0.0 3.0279173984133713E-4 9.909547849352852E-4 24-25 0.0 0.0 0.0 4.954773924676426E-4 0.001046007828542801 26-27 0.0 0.0 0.0 7.982691323089796E-4 0.0012386934811691064 28-29 2.752652180375792E-5 0.0 0.0 0.00291781131119834 0.0013212730465803802 30-31 5.505304360751584E-5 0.0 0.0 0.011175767852325717 0.001348799568384138 32-33 5.505304360751584E-5 0.0 0.0 0.030334227027741228 0.001376326090187896 34-35 5.505304360751584E-5 0.0 0.0 0.05252060360157011 0.0014313791337954118 36-37 5.505304360751584E-5 0.0 0.0 0.08610296020215477 0.0014313791337954118 38-39 5.505304360751584E-5 0.0 0.0 0.13631133597220924 0.0014864321774029276 40-41 5.505304360751584E-5 0.0 0.0 0.19549335785028876 0.0014864321774029276 42-43 5.505304360751584E-5 0.0 0.0 0.25613428538396743 0.0014864321774029276 44-45 5.505304360751584E-5 0.0 0.0 0.32280352119266914 0.0015139586992066857 46-47 5.505304360751584E-5 0.0 0.0 0.3963268609305065 0.0015414852210104437 48-49 5.505304360751584E-5 0.0 0.0 0.471887163281822 0.0015414852210104437 50-51 5.505304360751584E-5 0.0 0.0 0.5514388112946824 0.0016515913082254753 52-53 5.505304360751584E-5 0.0 0.0 0.6348992254036765 0.0017066443518329911 54-55 5.505304360751584E-5 0.0 0.0 0.7324256921543908 0.0017066443518329911 56-57 5.505304360751584E-5 0.0 0.0 0.8390909641439527 0.0017066443518329911 58-59 5.505304360751584E-5 0.0 0.0 0.9553079391994186 0.001761697395440507 60-61 5.505304360751584E-5 0.0 0.0 1.0704238533827342 0.0018167504390480228 62-63 5.505304360751584E-5 0.0 0.0 1.1886227380080707 0.0019268565262630544 64-65 5.505304360751584E-5 0.0 0.0 1.3157126891760211 0.0019268565262630544 66-67 5.505304360751584E-5 0.0 0.0 1.4669433999658672 0.0019268565262630544 68-69 5.505304360751584E-5 0.0 0.0 1.629790302956899 0.0019268565262630544 70-71 5.505304360751584E-5 0.0 0.0 1.8010052685762732 0.0019268565262630544 72-73 5.505304360751584E-5 0.0 0.0 1.9677058846198312 0.0020094360916743286 74-75 5.505304360751584E-5 0.0 0.0 2.148224814608876 0.0020369626134780863 76-77 5.505304360751584E-5 0.0 0.0 2.3608947220647094 0.0020369626134780863 78-79 5.505304360751584E-5 0.0 0.0 2.6019444735002173 0.0020369626134780863 80-81 5.505304360751584E-5 0.0 0.0 2.8428565923267066 0.002064489135281844 82-83 5.505304360751584E-5 0.0 0.0 3.0901548642116676 0.002147068700693118 84-85 5.505304360751584E-5 5.505304360751584E-5 0.0 3.3483811652527207 0.002147068700693118 86-87 5.505304360751584E-5 5.505304360751584E-5 0.0 3.6388685498477784 0.002147068700693118 88-89 5.505304360751584E-5 5.505304360751584E-5 0.0 3.9647550414824684 0.002147068700693118 90-91 5.505304360751584E-5 5.505304360751584E-5 0.0 4.309497200552732 0.002147068700693118 92-93 5.505304360751584E-5 1.1010608721503168E-4 0.0 4.65440451875382 0.002422333918730697 94-95 5.505304360751584E-5 1.1010608721503168E-4 0.0 5.013020044813178 0.002422333918730697 96-97 5.505304360751584E-5 1.1010608721503168E-4 0.0 5.410805811399284 0.0025874930495532445 98-99 5.505304360751584E-5 1.1010608721503168E-4 0.0 5.854258077657823 0.0028077052239833078 100-101 5.505304360751584E-5 1.1010608721503168E-4 0.0 6.329283264425274 0.0028077052239833078 102-103 5.505304360751584E-5 1.6515913082254752E-4 0.0 6.811960824254169 0.0028627582675908236 104-105 5.505304360751584E-5 1.6515913082254752E-4 0.0 7.311402035861553 0.0029178113111983394 106-107 5.505304360751584E-5 1.6515913082254752E-4 0.0 7.852105503652769 0.0029178113111983394 108-109 5.505304360751584E-5 1.6515913082254752E-4 0.0 8.432392109797789 0.0029178113111983394 110-111 5.505304360751584E-5 1.6515913082254752E-4 0.0 9.049646834725259 0.0029178113111983394 112-113 5.505304360751584E-5 1.6515913082254752E-4 0.0 9.67001205661655 0.0029178113111983394 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3120 0.0 47.23343 1 GTATCAA 5070 0.0 46.365547 1 TCAACGC 6345 0.0 36.75229 4 CAACGCA 6470 0.0 36.008446 5 ATCAACG 6505 0.0 35.94595 3 GTACATG 10615 0.0 35.859795 1 TACATGG 10840 0.0 34.777554 2 AACGCAG 6750 0.0 34.61258 6 TATCAAC 6830 0.0 34.584835 2 ACATGGG 10960 0.0 33.689247 3 CATGGGG 9110 0.0 32.471516 4 ATGGGGG 5610 0.0 30.270105 5 ACGCAGA 7920 0.0 29.420858 7 CGCAGAG 8410 0.0 27.986267 8 GCAGAGT 9385 0.0 24.888327 9 CGTATAC 155 2.0712656E-4 23.090364 3 GTATAGG 440 0.0 23.051722 1 AGAGTAC 8570 0.0 21.971018 10-11 GAGTACT 3410 0.0 21.738205 12-13 ATGGGGA 3160 0.0 21.684164 5 >>END_MODULE