FastQCFastQC Report
Mon 15 Aug 2016
SRR2921578_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921578_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7362907
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102250.040.9093741
GGTATCA58600.038.557261
TCAACGC125550.032.829364
CAACGCA128550.031.848535
TATCAAC135450.030.5664022
ATCAACG136700.030.0847993
AACGCAG139350.029.5810266
GTACATG241100.026.033911
TACATGG252050.024.1014582
ACGCAGA168500.023.8323257
ACATGGG257550.022.8014223
CGCAGAG178900.022.1381368
GAGTACA178550.020.8760071
AGTACAT171650.019.997482
CATGGGG204300.018.9828224
GCAGAGT195450.018.8526869
GTATAGG17900.018.5098021
AGAGTAC162800.017.37706610-11
GTGTAGC39200.016.7869661
GTATTAC12900.016.0525881
Produced by FastQC (version 0.11.5)