##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921578_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7362907
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.025378155665962	31.0	28.0	31.0	16.0	34.0
2	27.99355662104655	31.0	28.0	33.0	16.0	34.0
3	28.088196550628712	31.0	28.0	34.0	16.0	34.0
4	31.489849457557998	35.0	32.0	37.0	17.0	37.0
5	31.403137646584426	35.0	32.0	37.0	17.0	37.0
6	31.49746316230804	35.0	32.0	37.0	17.0	37.0
7	31.29295344895705	35.0	32.0	37.0	17.0	37.0
8	31.232168218340934	35.0	32.0	37.0	17.0	37.0
9	32.464003823489826	37.0	32.0	39.0	16.0	39.0
10-11	32.2276388252629	37.0	32.0	39.0	13.0	39.0
12-13	32.02819042804697	37.0	32.0	39.0	10.0	39.0
14-15	32.73420824139161	38.0	31.5	40.0	10.0	41.0
16-17	32.577729081733615	38.0	31.0	40.0	10.0	41.0
18-19	32.37982355338727	37.5	31.0	40.0	10.0	41.0
20-21	32.23141260646101	37.0	31.0	40.0	8.5	41.0
22-23	31.99729868922696	37.0	30.5	40.0	8.0	41.0
24-25	32.11029481969554	37.0	31.0	40.0	5.0	41.0
26-27	31.864196369721903	37.0	30.0	40.0	2.0	41.0
28-29	31.661968363854115	37.0	30.0	40.0	2.0	41.0
30-31	31.40288788110457	37.0	30.0	40.0	2.0	41.0
32-33	31.15388378530382	37.0	30.0	40.0	2.0	41.0
34-35	30.801934140958185	36.0	29.0	40.0	2.0	41.0
36-37	30.492865589637354	36.0	28.0	39.0	2.0	41.0
38-39	30.26459386217971	36.0	27.5	39.0	2.0	40.5
40-41	30.020830291622588	35.5	27.0	39.0	2.0	40.0
42-43	29.756570604518025	35.0	27.0	39.0	2.0	40.0
44-45	29.476943549606155	35.0	26.0	39.0	2.0	40.0
46-47	29.174912707711776	35.0	25.5	39.0	2.0	40.0
48-49	28.8712409378524	35.0	24.5	39.0	2.0	40.0
50-51	27.953828969997858	33.5	23.5	38.0	2.0	39.5
52-53	28.16379773641036	33.5	24.0	38.0	2.0	39.5
54-55	28.716521341366935	35.0	25.5	39.0	2.0	40.0
56-57	28.675014162205226	35.0	25.5	39.0	2.0	40.0
58-59	28.410728670075557	34.0	25.0	38.5	2.0	40.0
60-61	28.05198911245246	34.0	23.5	38.0	2.0	40.0
62-63	27.618462992945588	34.0	22.5	38.0	2.0	40.0
64-65	27.124255406186712	33.5	22.0	37.0	2.0	40.0
66-67	26.75558627862609	33.0	21.0	37.0	2.0	39.0
68-69	26.288221825971725	33.0	19.5	36.0	2.0	39.0
70-71	25.847802165639198	32.0	18.0	36.0	2.0	39.0
72-73	25.361863255912372	32.0	15.0	35.0	2.0	38.0
74-75	24.90043905212982	31.5	9.5	35.0	2.0	37.0
76-77	24.464625452963077	31.0	7.0	35.0	2.0	36.5
78-79	24.062106719533467	31.0	4.0	35.0	2.0	36.0
80-81	23.610955020890525	31.0	2.0	34.0	2.0	36.0
82-83	23.06968219210157	30.0	2.0	34.0	2.0	35.0
84-85	22.641754133251986	30.0	2.0	34.0	2.0	35.0
86-87	22.13864965291562	30.0	2.0	34.0	2.0	35.0
88-89	21.617415173110295	29.0	2.0	34.0	2.0	35.0
90-91	21.016847897168876	29.0	2.0	34.0	2.0	35.0
92-93	20.461385699968776	29.0	2.0	34.0	2.0	35.0
94-95	19.774620947948954	27.0	2.0	34.0	2.0	35.0
96-97	19.12312086788547	26.5	2.0	33.0	2.0	35.0
98	18.63760074655296	25.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	484606.0
3	81446.0
4	58369.0
5	47301.0
6	43694.0
7	46190.0
8	52631.0
9	59744.0
10	63548.0
11	66481.0
12	67118.0
13	65596.0
14	64100.0
15	66351.0
16	69965.0
17	74522.0
18	76803.0
19	79015.0
20	82747.0
21	87279.0
22	93656.0
23	103289.0
24	117059.0
25	131724.0
26	150629.0
27	172558.0
28	198627.0
29	227218.0
30	261323.0
31	303519.0
32	357717.0
33	427821.0
34	522449.0
35	643917.0
36	778517.0
37	759571.0
38	352822.0
39	22985.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.346565880118334	16.80712561695628	12.561804570910004	25.28450393201538
2	16.39735596488854	18.204376216739618	38.86811851511358	26.53014930325826
3	20.725162909251825	21.677303348538697	27.42162833231815	30.175905409891335
4	13.860926076139746	14.208233525503777	34.42698010461379	37.50386029374268
5	15.426745176171815	35.95955895651001	33.1376850791962	15.476010788121968
6	36.18337747738775	32.769884628507164	16.093663569721087	14.953074324384
7	31.744981943965158	29.917382431980784	18.857211952578794	19.48042367147527
8	28.072623061334628	34.14604726812524	18.341463189349465	19.439866481190673
9	27.121606720966916	15.682776815967458	18.245979435158212	38.94963702790741
10-11	27.032091945680314	24.437651420346278	26.083829454681666	22.446427179291742
12-13	27.233101442212547	21.370003451270065	26.40831445049899	24.988580656018392
14-15	24.27934766213179	23.014903289677026	23.5063686755794	29.199380372611785
16-17	24.894392728050622	26.222793432291517	24.250565786591917	24.632248053065943
18-19	24.986250740965318	25.494893496320227	25.44823263848196	24.070623124232498
20-21	25.417346120281625	24.690928046125563	25.40267801072974	24.489047822863068
22-23	25.241952229783166	24.373373173000424	25.267889701851615	25.116784895364795
24-25	24.990017515895865	24.713280495707462	25.275554615672323	25.021147372724347
26-27	24.88946508488542	24.833532450340755	25.28848972304103	24.988512741732794
28-29	24.96044972237656	24.93597932632184	25.237359443064662	24.86621150823694
30-31	25.137180070035807	24.831702720903984	25.059315693226043	24.97180151583417
32-33	24.896620379737698	24.823585801502027	25.09223731340881	25.187556505351466
34-35	25.110303040868992	24.83681660398205	25.192112661783923	24.860767693365037
36-37	25.238680491552923	24.675385398262478	25.093925682534806	24.99200842764979
38-39	25.142786938563134	24.788176405401725	25.05375442138846	25.01528223464668
40-41	25.24318204814684	24.667540420185276	25.17089467295397	24.91838285871392
42-43	25.14155926646413	24.580758403429467	25.07131780368686	25.206364526419538
44-45	25.00007492324454	24.67599783458201	25.19232115753416	25.13160608463929
46-47	25.280408596258898	24.674655460449355	25.026500422521835	25.018435520769916
48-49	25.07730630241844	24.724081206081845	24.935015028072748	25.26359746342697
50-51	24.93822177334434	24.845588489715116	25.104348669499977	25.111841067440572
52-53	25.087420164109233	24.75863203422613	25.15234645015122	25.00160135151342
54-55	24.80142306458304	24.54819109852881	25.34891702062614	25.30146881626201
56-57	24.865531038970452	24.727621841590537	25.295308525845776	25.11153859359323
58-59	24.94945314036222	24.59221106011945	25.311265467236883	25.14707033228145
60-61	24.916176458567765	24.48594222128979	25.40902972854414	25.1888515915983
62-63	24.759409776324638	24.55291663473737	25.51878549473379	25.168888094204195
64-65	24.74220145960259	24.475859821251685	25.44882602573721	25.333112693408516
66-67	24.70255677903978	24.737247074628904	25.252763537562483	25.30743260876883
68-69	24.737554409451697	24.507591184086852	25.39160194544761	25.36325246101384
70-71	24.695493305000014	24.355925215615486	25.517680790060528	25.43090068932397
72-73	24.745774321774906	24.398488430737455	25.42094047312562	25.434796774362017
74-75	24.466249228298192	24.53737378743758	25.52654095949461	25.46983602476962
76-77	24.57988559838082	24.2822801674085	25.595095120447407	25.542739113763268
78-79	24.54302858540792	24.144008202100785	25.660039181671863	25.65292403081943
80-81	24.48583831228703	24.362856300789055	25.572183952603993	25.57912143431992
82-83	24.51973752880179	24.306998675905753	25.59495897772598	25.578304817566476
84-85	24.403782604992532	24.282349919897047	25.630262256064878	25.683605219045543
86-87	24.345855562511794	24.22756696802402	25.8127158298801	25.61386163958408
88-89	24.32996886347425	24.044105254735594	25.731899021642352	25.8940268601478
90-91	24.293200247663314	24.017758554413145	25.830113968970736	25.858927228952805
92-93	24.327953987957265	24.063375483626416	25.88672331122885	25.721947217187473
94-95	24.25773196788802	23.829427413220923	25.985861353788277	25.926979265102784
96-97	24.187778798474746	23.740965901307348	26.061207865497497	26.010047434720406
98	24.212674258285247	23.758212054673272	26.00718310098895	26.021930586052527
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	50.5
2	98.0
3	192.0
4	225.5
5	285.5
6	446.0
7	795.0
8	1204.0
9	1422.0
10	1519.0
11	1627.0
12	1856.0
13	2128.0
14	2440.0
15	2624.0
16	2682.5
17	2751.5
18	2714.5
19	2605.5
20	2526.5
21	2442.0
22	2466.0
23	2701.5
24	2987.0
25	3603.0
26	4537.0
27	5850.5
28	7785.0
29	10787.5
30	15680.5
31	23060.5
32	33158.0
33	44907.0
34	59157.0
35	76640.0
36	95889.0
37	115290.0
38	133713.5
39	151494.0
40	169072.5
41	187283.5
42	207890.5
43	231896.0
44	255092.5
45	275900.0
46	295633.5
47	315129.0
48	331269.5
49	340309.0
50	343406.0
51	346796.5
52	354326.0
53	360959.5
54	360075.5
55	351310.5
56	333457.5
57	310171.5
58	286457.0
59	255648.0
60	219634.5
61	182738.0
62	147686.0
63	113382.0
64	82443.5
65	60915.5
66	45332.5
67	33172.0
68	23931.5
69	17467.5
70	12742.0
71	9221.5
72	6903.0
73	5020.0
74	3696.5
75	2674.5
76	1785.0
77	1107.5
78	670.5
79	422.0
80	272.5
81	177.5
82	108.0
83	69.0
84	52.5
85	36.0
86	24.5
87	27.5
88	30.0
89	20.5
90	12.5
91	12.5
92	12.0
93	13.5
94	9.5
95	6.5
96	10.0
97	9.5
98	6.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2661856247810817
2	0.1324476867628506
3	0.13011165291100377
4	0.1248419951521865
5	0.12078109909577835
6	0.044683438212651604
7	0.03377741970664576
8	0.012875349369481374
9	0.008597147838482816
10-11	0.0016976990202375228
12-13	0.005432636864760074
14-15	0.010057168995887086
16-17	0.005018398303822118
18-19	0.04644225439761768
20-21	0.092524596602945
22-23	0.09173686425755478
24-25	0.06816601106057703
26-27	0.07935045220590183
28-29	0.0961576725062533
30-31	0.07119470611268078
32-33	0.018002400410598692
34-35	0.2833255940893997
36-37	0.3674363943480476
38-39	0.3414208545619278
40-41	0.3372920505447101
42-43	0.3154324779601318
44-45	0.29962350468367993
46-47	0.30501539677195433
48-49	0.29850981412640415
50-51	0.3006013793193368
52-53	0.3443612692649792
54-55	0.3310988445188836
56-57	0.3163831894114648
58-59	0.29806841238114234
60-61	0.30540926294464943
62-63	0.3051715850818162
64-65	0.32119107303677746
66-67	0.3411695951069326
68-69	0.3040307313402166
70-71	0.28230697467725724
72-73	0.26725177976579084
74-75	0.25464127144346654
76-77	0.257439079428818
78-79	0.2637884737645063
80-81	0.2545665726865761
82-83	0.2634353523682969
84-85	0.2569705144992324
86-87	0.2523595639602673
88-89	0.2749525425215883
90-91	0.25894663615878893
92-93	0.2498333878181539
94-95	0.26372056580369685
96-97	0.26268157400331144
98	0.2618802600657594
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	7362907.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.22218486374126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.23703478847868	38.2879045461045
2	11.472947240258966	11.523929544644718
3	4.484838512420475	6.757151665644223
4	2.3165743896261657	4.653737089856555
5	1.3104591956680725	3.290706199061581
6	0.848141253191845	2.5557304085019608
7	0.5935606416911097	2.086693859239626
8	0.4404511262345064	1.7696334308153934
9	0.31496492012003174	1.423640379947556
>10	1.7781852575781238	16.347817739934346
>50	0.135067754270969	4.661013034760318
>100	0.06531663329601083	5.669871042095282
>500	0.0020213156702692378	0.6402566212667585
>1k	4.369714947982956E-4	0.33191443812719906
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3581592161900184E-5	0.0	1.3581592161900184E-5	0.0
3	0.0	1.3581592161900184E-5	0.0	1.3581592161900184E-5	0.0
4	0.0	1.3581592161900184E-5	0.0	1.3581592161900184E-5	1.3581592161900184E-5
5	1.3581592161900184E-5	1.3581592161900184E-5	0.0	1.3581592161900184E-5	2.7163184323800368E-5
6	1.3581592161900184E-5	1.3581592161900184E-5	0.0	1.3581592161900184E-5	4.0744776485700555E-5
7	1.3581592161900184E-5	1.3581592161900184E-5	0.0	2.7163184323800368E-5	5.4326368647600735E-5
8	1.3581592161900184E-5	1.3581592161900184E-5	0.0	2.7163184323800368E-5	8.148955297140111E-5
9	1.3581592161900184E-5	1.3581592161900184E-5	0.0	4.0744776485700555E-5	1.2223432945710166E-4
10-11	1.3581592161900184E-5	1.3581592161900184E-5	0.0	6.790796080950092E-5	1.2902512553805175E-4
12-13	2.0372388242850277E-5	4.0744776485700555E-5	6.790796080950092E-6	1.0186194121425139E-4	1.6297910594280222E-4
14-15	2.7163184323800368E-5	4.0744776485700555E-5	1.3581592161900184E-5	1.2223432945710166E-4	2.1730547459040294E-4
16-17	2.7163184323800368E-5	4.0744776485700555E-5	1.3581592161900184E-5	1.3581592161900183E-4	2.7163184323800366E-4
18-19	2.7163184323800368E-5	4.0744776485700555E-5	1.3581592161900184E-5	1.4939751378090203E-4	2.7163184323800366E-4
20-21	2.7163184323800368E-5	4.0744776485700555E-5	1.3581592161900184E-5	1.6297910594280222E-4	2.7163184323800366E-4
22-23	2.7163184323800368E-5	4.7535572566650645E-5	1.3581592161900184E-5	2.512594549951534E-4	3.1237661972370427E-4
24-25	2.7163184323800368E-5	5.4326368647600735E-5	1.3581592161900184E-5	3.5991219229035486E-4	3.66702988371305E-4
26-27	2.7163184323800368E-5	6.790796080950092E-5	1.3581592161900184E-5	6.111716472855083E-4	3.8028458053320516E-4
28-29	2.7163184323800368E-5	6.790796080950092E-5	1.3581592161900184E-5	0.002505803753870584	3.938661726951053E-4
30-31	2.7163184323800368E-5	8.148955297140111E-5	1.3581592161900184E-5	0.010457825964663141	4.0065696877605546E-4
32-33	2.7163184323800368E-5	8.148955297140111E-5	1.3581592161900184E-5	0.027944125873109626	4.0744776485700555E-4
34-35	2.7163184323800368E-5	8.148955297140111E-5	1.3581592161900184E-5	0.05004137632052123	4.0744776485700555E-4
36-37	2.7163184323800368E-5	8.148955297140111E-5	1.3581592161900184E-5	0.087900064471818	4.0744776485700555E-4
38-39	2.7163184323800368E-5	8.828034905235119E-5	1.3581592161900184E-5	0.15375041406879103	4.1423856093795563E-4
40-41	2.7163184323800368E-5	1.0865273729520147E-4	1.3581592161900184E-5	0.23061543490906514	4.346109491808059E-4
42-43	2.7163184323800368E-5	1.1544353337615157E-4	1.3581592161900184E-5	0.3114734438449379	4.481925413427061E-4
44-45	2.7163184323800368E-5	1.2223432945710166E-4	1.3581592161900184E-5	0.4074409740609246	4.549833374236562E-4
46-47	2.7163184323800368E-5	1.2223432945710166E-4	1.3581592161900184E-5	0.5224634780800572	4.6177413350460627E-4
48-49	2.7163184323800368E-5	1.2223432945710166E-4	1.3581592161900184E-5	0.6513459969004091	4.7535572566650644E-4
50-51	2.7163184323800368E-5	1.2223432945710166E-4	1.3581592161900184E-5	0.7937489907179325	4.7535572566650644E-4
52-53	3.395398040475046E-5	1.2223432945710166E-4	1.3581592161900184E-5	0.9413876883138684	5.025189099903068E-4
54-55	4.0744776485700555E-5	1.2223432945710166E-4	1.3581592161900184E-5	1.1094734729095452	5.568452786379076E-4
56-57	4.0744776485700555E-5	1.2223432945710166E-4	2.7163184323800368E-5	1.3048922117310457	5.568452786379076E-4
58-59	4.0744776485700555E-5	1.2223432945710166E-4	2.7163184323800368E-5	1.519053547735969	5.840084629617079E-4
60-61	4.0744776485700555E-5	1.2223432945710166E-4	2.7163184323800368E-5	1.7558825610591033	5.975900551236081E-4
62-63	4.0744776485700555E-5	1.2902512553805175E-4	2.7163184323800368E-5	1.9988708264276598	5.975900551236081E-4
64-65	4.0744776485700555E-5	1.3581592161900183E-4	2.7163184323800368E-5	2.25639818620553	5.975900551236081E-4
66-67	4.0744776485700555E-5	1.3581592161900183E-4	2.7163184323800368E-5	2.5548468831672055	6.043808512045582E-4
68-69	4.0744776485700555E-5	1.4939751378090203E-4	2.7163184323800368E-5	2.8910591971350446	6.111716472855083E-4
70-71	4.0744776485700555E-5	1.4939751378090203E-4	2.7163184323800368E-5	3.262033596241267	6.383348316093087E-4
72-73	4.0744776485700555E-5	1.6297910594280222E-4	2.7163184323800368E-5	3.640722339695449	7.198243845807098E-4
74-75	4.0744776485700555E-5	1.697699020237523E-4	2.7163184323800368E-5	4.029773837969161	7.198243845807098E-4
76-77	4.7535572566650645E-5	1.7656069810470239E-4	2.7163184323800368E-5	4.459943606513026	7.3340597674261E-4
78-79	8.148955297140111E-5	1.7656069810470239E-4	3.395398040475046E-5	4.936881587666393	7.3340597674261E-4
80-81	9.507114513330129E-5	1.969330863475527E-4	4.0744776485700555E-5	5.45698186871028	7.673599571473604E-4
82-83	9.507114513330129E-5	2.1730547459040294E-4	4.0744776485700555E-5	5.991166260826057	8.284771218759113E-4
84-85	9.507114513330129E-5	2.2409627067135302E-4	4.0744776485700555E-5	6.53003630223769	8.284771218759113E-4
86	9.507114513330129E-5	2.3088706675230313E-4	4.0744776485700555E-5	6.945205202238736	8.284771218759113E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10225	0.0	40.909374	1
GGTATCA	5860	0.0	38.55726	1
TCAACGC	12555	0.0	32.82936	4
CAACGCA	12855	0.0	31.84853	5
TATCAAC	13545	0.0	30.566402	2
ATCAACG	13670	0.0	30.084799	3
AACGCAG	13935	0.0	29.581026	6
GTACATG	24110	0.0	26.03391	1
TACATGG	25205	0.0	24.101458	2
ACGCAGA	16850	0.0	23.832325	7
ACATGGG	25755	0.0	22.801422	3
CGCAGAG	17890	0.0	22.138136	8
GAGTACA	17855	0.0	20.876007	1
AGTACAT	17165	0.0	19.99748	2
CATGGGG	20430	0.0	18.982822	4
GCAGAGT	19545	0.0	18.852686	9
GTATAGG	1790	0.0	18.509802	1
AGAGTAC	16280	0.0	17.377066	10-11
GTGTAGC	3920	0.0	16.786966	1
GTATTAC	1290	0.0	16.052588	1
>>END_MODULE