FastQCFastQC Report
Mon 15 Aug 2016
SRR2921578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7362907
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62650.039.7969551
GTATCAA100300.038.4733121
TCAACGC127600.029.4403744
CAACGCA132400.028.3371815
TATCAAC137000.027.6624722
ATCAACG137800.027.3990463
AACGCAG144450.025.8711436
GTACATG242350.024.5810781
TACATGG256200.022.8741362
ACATGGG264500.021.3308563
ACGCAGA179300.020.4718767
GAGTACA188200.020.1501521
AGTACAT179900.019.402822
CGCAGAG188750.019.0438848
CATGGGG199750.017.7841194
GCAGAGT209950.016.4634469
GTATAGG18300.016.3805731
AGAGTAC171550.016.31409510-11
CAGAGTA189100.015.4705844
GTGGTAT44550.014.3118811
Produced by FastQC (version 0.11.5)