FastQCFastQC Report
Mon 15 Aug 2016
SRR2921577_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921577_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6493615
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56850.040.876611
GTATCAA99900.039.9823341
TCAACGC125150.031.1335494
TATCAAC129950.030.2315332
CAACGCA128600.030.1901995
ATCAACG136900.028.528953
AACGCAG138600.028.2830586
GTACATG212400.026.539671
TACATGG224650.024.7689762
ACGCAGA165750.022.8417027
ACATGGG237450.022.79523
CGCAGAG173350.021.3884228
CATGGGG180600.019.9971644
GAGTACA167700.019.0980971
AGTACAT160200.018.3277282
GCAGAGT194500.017.3857049
GTATAGG12400.016.699551
AGAGTAC152900.016.53440510-11
GTATTAA20450.015.9764031
GTATAGA16950.015.4745881
Produced by FastQC (version 0.11.5)