##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921577_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6493615
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.43001579243611	31.0	28.0	31.0	20.0	34.0
2	28.40111201541822	31.0	28.0	33.0	16.0	34.0
3	28.497118015157966	31.0	28.0	34.0	16.0	34.0
4	31.944362269706474	35.0	32.0	37.0	20.0	37.0
5	31.883595963111457	35.0	32.0	37.0	20.0	37.0
6	31.97994861105871	35.0	32.0	37.0	22.0	37.0
7	31.78749094302634	35.0	32.0	37.0	19.0	37.0
8	31.746343446601006	35.0	32.0	37.0	18.0	37.0
9	33.01415451947798	37.0	32.0	39.0	18.0	39.0
10-11	32.79077978906973	37.0	32.0	39.0	17.0	39.0
12-13	32.60705985802977	37.0	32.0	39.0	17.0	39.0
14-15	33.34481317417186	38.0	32.0	40.0	17.0	41.0
16-17	33.201314599033054	38.0	32.0	40.0	16.5	41.0
18-19	33.02768419439711	38.0	32.0	40.0	14.0	41.0
20-21	32.894915466962544	38.0	32.0	40.0	11.0	41.0
22-23	32.670495479020545	37.5	31.0	40.0	10.0	41.0
24-25	32.779941681174506	38.0	31.5	40.0	10.0	41.0
26-27	32.55028316276835	38.0	31.0	40.0	10.0	41.0
28-29	32.365325477411275	37.0	31.0	40.0	9.0	41.0
30-31	32.12125518682583	37.0	30.0	40.0	8.0	41.0
32-33	31.883340096386988	37.0	30.0	40.0	8.0	41.0
34-35	31.5465723637758	37.0	30.0	40.0	2.0	41.0
36-37	31.24865125203758	37.0	30.0	40.0	2.0	41.0
38-39	31.033799894203767	36.0	29.5	40.0	2.0	41.0
40-41	30.806872212165334	36.0	29.0	39.0	2.0	41.0
42-43	30.558849269628706	36.0	28.5	39.0	2.0	40.0
44-45	30.291452449829563	35.5	28.0	39.0	2.0	40.0
46-47	29.996617754517324	35.0	27.0	39.0	2.0	40.0
48-49	29.707920780643754	35.0	27.0	39.0	2.0	40.0
50-51	28.759964287996745	34.0	25.0	38.0	2.0	39.5
52-53	28.9605228366634	34.5	26.5	38.0	2.0	39.5
54-55	29.54527339548156	35.0	27.0	39.0	2.0	40.0
56-57	29.518113793318513	35.0	27.0	39.0	2.0	40.0
58-59	29.262829564117986	35.0	26.5	39.0	2.0	40.0
60-61	28.906270159225638	34.0	26.0	38.0	2.0	40.0
62-63	28.4775569540233	34.0	26.0	38.0	2.0	40.0
64-65	27.99664431907343	34.0	25.0	37.5	2.0	40.0
66-67	27.63477515990708	34.0	24.5	37.0	2.0	39.5
68-69	27.16563947816432	33.0	23.0	36.0	2.0	39.0
70-71	26.732919490915307	33.0	22.0	36.0	2.0	39.0
72-73	26.251628176293174	32.5	21.0	35.0	2.0	38.0
74-75	25.80160796413092	32.0	20.0	35.0	2.0	37.0
76-77	25.371172451708333	32.0	18.0	35.0	2.0	37.0
78-79	24.96673809580642	32.0	17.0	35.0	2.0	36.0
80-81	24.52567853191173	31.0	10.5	34.5	2.0	36.0
82-83	23.991042662677106	31.0	7.0	34.0	2.0	35.0
84-85	23.573833835236613	31.0	2.0	34.0	2.0	35.0
86-87	23.072887598048236	30.5	2.0	34.0	2.0	35.0
88-89	22.554553819405676	30.0	2.0	34.0	2.0	35.0
90-91	21.955995543314472	29.5	2.0	34.0	2.0	35.0
92-93	21.403919773500583	29.0	2.0	34.0	2.0	35.0
94-95	20.72047064693549	29.0	2.0	34.0	2.0	35.0
96-97	20.068643659964444	29.0	2.0	34.0	2.0	35.0
98	19.57687559240885	27.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	357000.0
3	61735.0
4	44908.0
5	36806.0
6	33921.0
7	35629.0
8	41311.0
9	47691.0
10	50068.0
11	52980.0
12	54419.0
13	52908.0
14	51660.0
15	53786.0
16	57213.0
17	61427.0
18	64025.0
19	64969.0
20	67556.0
21	72313.0
22	78969.0
23	88119.0
24	99000.0
25	114306.0
26	130954.0
27	151129.0
28	174039.0
29	201440.0
30	232880.0
31	272617.0
32	321828.0
33	387809.0
34	475353.0
35	591570.0
36	726073.0
37	722762.0
38	340482.0
39	21960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84524764299164	17.191113702472645	12.6865710820729	25.27706757246282
2	16.60117320773721	19.06288328091081	37.96202263456408	26.3739208767879
3	20.012058094728516	22.374581166242884	28.287951002810985	29.325409736217612
4	14.049256739150213	14.818760301652429	34.71801418833387	36.4139687708635
5	15.409465928218207	36.05361886975202	33.219881246115584	15.317033955914189
6	35.22549867432567	33.104958987985654	16.629467054115768	15.040075283572902
7	30.686993525631586	30.572735434519654	19.736332933011518	19.003938106837246
8	27.87197117786689	33.32800947759268	19.259199591916445	19.54081975262399
9	27.351881764149272	15.297095851321782	18.558025681589683	38.79299670293926
10-11	26.67242934976699	24.479737670098867	26.612315417425698	22.23551756270845
12-13	26.898629419277913	21.604201088527127	26.733412189344673	24.76375730285028
14-15	24.344191088858143	23.18525118873776	23.885075374063412	28.58548234834069
16-17	24.737087986827007	26.23818500483076	24.553105597021048	24.47162141132119
18-19	24.77288563336534	25.70991559596842	25.683515503217585	23.833683267448656
20-21	25.347479557396518	24.846318235011942	25.554911404081	24.251290803510543
22-23	25.23779877158046	24.448668621290437	25.373836824933026	24.93969578219607
24-25	24.964372726124644	24.692823359070758	25.38102016170448	24.96178375310012
26-27	24.77391717129061	24.89368604864426	25.409828106770156	24.92256867329498
28-29	24.89837601241508	25.03525942528235	25.328387238242268	24.737977324060303
30-31	25.13116123784061	24.90540525233652	25.093997570761417	24.86943593906145
32-33	24.940856188450024	24.952908672590222	25.033810491052087	25.07242464790766
34-35	25.132340095357762	24.894115568470905	25.2044174935413	24.769126842630033
36-37	25.192119159361543	24.7127535218207	25.232547400392995	24.86257991842476
38-39	25.107307892929853	24.842720814968995	25.18081803951669	24.869153252584464
40-41	25.1687830575821	24.77560363768805	25.185193540945004	24.870419763784845
42-43	24.988384348861164	24.707067375411278	25.088686413724197	25.215861862003365
44-45	24.906407928053447	24.721784035999537	25.213190201224982	25.158617834722037
46-47	25.058160815262436	24.661830803061427	25.17467939818453	25.105328983491603
48-49	24.90616349084789	24.77276118446234	25.113646438862002	25.207428885827767
50-51	24.774437385183607	24.866275054553473	25.305676326394366	25.05361123386855
52-53	24.880530028113988	24.781572799472723	25.261191218791907	25.07670595362138
54-55	24.590415715378786	24.657574991563568	25.444944690361204	25.307064602696443
56-57	24.775196701437892	24.724863627185485	25.374558871080744	25.125380800295883
58-59	24.805016561824157	24.59104006433042	25.430287305114067	25.173656068731358
60-61	24.855900552516058	24.580457375318943	25.493871810249914	25.069770261915085
62-63	24.698307985177056	24.63452598768833	25.484491775729275	25.18267425140534
64-65	24.59821722739161	24.50817555376286	25.5493326170787	25.34427460176683
66-67	24.60952956101422	24.618306912295484	25.406730945826823	25.365432580863473
68-69	24.47849318773689	24.45678996517135	25.53255923628136	25.532157610810398
70-71	24.470868890199533	24.337289818941247	25.633892498306228	25.557948792552992
72-73	24.413086131193452	24.45883065870035	25.5149540597554	25.613129150350794
74-75	24.206530066262218	24.508838513668532	25.717933349945977	25.566698070123273
76-77	24.345223542315797	24.225665518692455	25.685551821613593	25.743559117378155
78-79	24.190561481999033	24.20607167190042	25.81226030715426	25.79110653894629
80-81	24.21707255127949	24.273757701283234	25.76821385810263	25.740955889334654
82-83	24.15682483973536	24.179561245275632	25.85173315875068	25.811880756238324
84-85	24.120316024334695	24.228208094591764	25.81507688236383	25.836398998709708
86-87	24.0267834156806	24.157211301125457	26.030645458240265	25.785359824953673
88-89	23.870018927056055	23.9904315404298	26.036110194643765	26.103439337870384
90-91	23.99407815342885	23.851073139761382	26.112735567641277	26.04211313916849
92-93	24.007248697065535	23.837486657253283	26.12875700407361	26.026507641607573
94-95	23.937537868423863	23.740768751393524	26.206462507095026	26.115230873087587
96-97	23.806715830995824	23.568074051753868	26.357923140995513	26.267286976254788
98	23.808717228558393	23.5131865510381	26.238244973932616	26.439851246470887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	132.0
1	116.0
2	174.0
3	277.0
4	299.0
5	357.5
6	552.5
7	993.5
8	1425.0
9	1593.5
10	1579.5
11	1623.0
12	1841.5
13	2127.0
14	2348.5
15	2569.5
16	2705.0
17	2705.0
18	2657.5
19	2572.0
20	2499.0
21	2476.0
22	2566.5
23	2670.0
24	2945.0
25	3437.5
26	4090.5
27	5212.5
28	7153.5
29	10180.0
30	14714.5
31	21222.0
32	29582.0
33	39185.5
34	50635.5
35	64391.5
36	79623.0
37	96571.5
38	114626.0
39	132428.0
40	150018.0
41	166938.5
42	186469.0
43	207754.0
44	227440.0
45	247894.0
46	268778.5
47	287877.0
48	303186.0
49	312987.0
50	316367.0
51	319882.0
52	324985.0
53	327188.5
54	324683.0
55	315831.5
56	297905.0
57	272637.5
58	245827.5
59	215276.0
60	182967.0
61	150791.5
62	120015.5
63	91046.0
64	65000.0
65	46740.0
66	34304.0
67	24882.5
68	17670.0
69	12522.0
70	8940.0
71	6430.0
72	4593.0
73	3124.5
74	2217.5
75	1480.0
76	899.0
77	567.5
78	330.5
79	191.5
80	118.0
81	83.0
82	63.5
83	51.0
84	39.5
85	28.5
86	24.0
87	19.0
88	16.0
89	19.5
90	17.0
91	13.5
92	13.0
93	11.0
94	8.0
95	8.5
96	9.0
97	5.5
98	3.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2679093232352087
2	0.13072841552817652
3	0.12851085258365333
4	0.12327494007575134
5	0.11956360209220904
6	0.04552163933340674
7	0.033432841337221256
8	0.01273558718833808
9	0.008608456152697688
10-11	0.0017247711790735977
12-13	0.005320611092588643
14-15	0.010756720255204535
16-17	0.005089614952534143
18-19	0.047854700347957184
20-21	0.09521660893046477
22-23	0.09381523234746747
24-25	0.07015352773455155
26-27	0.08138764001253539
28-29	0.09767286788637762
30-31	0.07315647755526006
32-33	0.018133196994278226
34-35	0.2869126056903589
36-37	0.37155729127766274
38-39	0.34494653594338437
40-41	0.34098110220578215
42-43	0.3182741816384248
44-45	0.30248944540136735
46-47	0.3075251612545554
48-49	0.3016655591685063
50-51	0.3032517326635472
52-53	0.34861167469891574
54-55	0.3339742192907957
56-57	0.3192982645259998
58-59	0.30090327190632643
60-61	0.30872634118283887
62-63	0.308017953020005
64-65	0.32426468153717153
66-67	0.3451775320834389
68-69	0.3067936734777162
70-71	0.2837248589576068
72-73	0.2684252146146638
74-75	0.25595912292305595
76-77	0.2593855656671977
78-79	0.2652990668525929
80-81	0.25578972575368264
82-83	0.2651450694258899
84-85	0.25828448406627125
86-87	0.2536722611365164
88-89	0.276648677200604
90-91	0.26054824623880535
92-93	0.2512160021806036
94-95	0.26553776286398256
96-97	0.2644366812630561
98	0.2637975919422387
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	6493615.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.908382773468766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32350557705823	38.34597853756389
2	11.779349558209296	11.993352722636178
3	4.727486793943503	7.220061217877833
4	2.461006440892078	5.011434316036237
5	1.3814642219413382	3.5164054699220917
6	0.9231385932455051	2.8197295714742197
7	0.6200445329006664	2.2095825092252634
8	0.434167980315026	1.7682231783888953
9	0.33382831795555157	1.5295193811994008
>10	1.8386630098810652	17.154057475174124
>50	0.13066031026571084	4.524438177392523
>100	0.04641064713335752	3.7499629758759117
>500	1.8268337539196312E-4	0.05345968706573316
>1k	9.1332883339354E-5	0.10379478016767521
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.079948534059996E-5
2	0.0	0.0	0.0	0.0	3.079948534059996E-5
3	0.0	0.0	0.0	0.0	3.079948534059996E-5
4	0.0	0.0	0.0	0.0	4.619922801089994E-5
5	0.0	0.0	0.0	0.0	4.619922801089994E-5
6	0.0	0.0	0.0	0.0	9.239845602179988E-5
7	0.0	0.0	0.0	0.0	9.239845602179988E-5
8	0.0	0.0	0.0	0.0	1.0779819869209986E-4
9	0.0	0.0	0.0	3.079948534059996E-5	1.6939716937329976E-4
10-11	0.0	0.0	0.0	3.079948534059996E-5	1.6939716937329976E-4
12-13	1.539974267029998E-5	0.0	0.0	4.619922801089994E-5	1.7709704070844977E-4
14-15	1.539974267029998E-5	0.0	0.0	4.619922801089994E-5	2.1559639738419968E-4
16-17	1.539974267029998E-5	0.0	0.0	4.619922801089994E-5	2.4639588272479967E-4
18-19	1.539974267029998E-5	0.0	0.0	4.619922801089994E-5	2.6179562539509964E-4
20-21	3.079948534059996E-5	0.0	0.0	8.46985846866499E-5	2.6179562539509964E-4
22-23	3.079948534059996E-5	0.0	0.0	2.1559639738419968E-4	2.848952394005496E-4
24-25	3.079948534059996E-5	0.0	0.0	4.927917654495993E-4	3.079948534059996E-4
26-27	3.079948534059996E-5	0.0	0.0	0.0010548823729155485	3.3109446741144954E-4
28-29	3.079948534059996E-5	0.0	0.0	0.004643022415095444	3.387943387465995E-4
30-31	3.079948534059996E-5	0.0	0.0	0.017809802398201926	3.387943387465995E-4
32-33	3.079948534059996E-5	0.0	0.0	0.04683061746038224	3.618939527520495E-4
34-35	3.849935667574995E-5	7.69987133514999E-6	0.0	0.08593826397160903	4.0039330942779944E-4
36-37	4.619922801089994E-5	1.539974267029998E-5	0.0	0.15131787147836762	4.0039330942779944E-4
38-39	4.619922801089994E-5	1.539974267029998E-5	0.0	0.2557820258823475	4.311927947683994E-4
40-41	4.619922801089994E-5	2.309961400544997E-5	0.0	0.3740751492042568	4.311927947683994E-4
42-43	4.619922801089994E-5	3.079948534059996E-5	1.539974267029998E-5	0.49739628850801904	4.465925374386994E-4
44-45	4.619922801089994E-5	3.079948534059996E-5	1.539974267029998E-5	0.6490221548397926	4.8509189411444936E-4
46-47	4.619922801089994E-5	3.079948534059996E-5	1.539974267029998E-5	0.8290682462696048	5.312911221253493E-4
48-49	4.619922801089994E-5	3.079948534059996E-5	1.539974267029998E-5	1.0221348201271556	6.005899641416992E-4
50-51	5.389909934604993E-5	3.079948534059996E-5	1.539974267029998E-5	1.2287839670199112	6.082898354768492E-4
52-53	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	1.4436103772705957	6.852885488283491E-4
54-55	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	1.6862179232985017	7.62287262179849E-4
56-57	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	1.9698195843147461	7.853868761852989E-4
58-59	6.92988420163499E-5	3.079948534059996E-5	1.539974267029998E-5	2.2747344891866854	8.16186361525899E-4
60-61	7.69987133514999E-5	3.079948534059996E-5	1.539974267029998E-5	2.5982599830756827	8.16186361525899E-4
62-63	7.69987133514999E-5	3.079948534059996E-5	1.539974267029998E-5	2.93361247933547	8.777853322070988E-4
64-65	7.69987133514999E-5	4.619922801089994E-5	2.309961400544997E-5	3.2996566627371657	8.931850748773988E-4
66-67	8.46985846866499E-5	4.619922801089994E-5	3.079948534059996E-5	3.714872224485129	9.085848175476987E-4
68-69	9.239845602179988E-5	4.619922801089994E-5	3.079948534059996E-5	4.1790050688253	9.316844315531488E-4
70-71	9.239845602179988E-5	4.619922801089994E-5	3.079948534059996E-5	4.671966231444273	0.0010317827589100986
72-73	9.239845602179988E-5	4.619922801089994E-5	3.079948534059996E-5	5.179264554489294	0.0012011799282833984
74-75	9.239845602179988E-5	4.619922801089994E-5	3.079948534059996E-5	5.7085537100675054	0.0012011799282833984
76-77	9.239845602179988E-5	4.619922801089994E-5	3.079948534059996E-5	6.29306634286141	0.0012165796709536984
78-79	9.239845602179988E-5	6.159897068119992E-5	3.079948534059996E-5	6.9302845949444185	0.0012319794136239984
80-81	1.0779819869209986E-4	6.159897068119992E-5	3.079948534059996E-5	7.611576910549825	0.0012704787702997483
82-83	1.0779819869209986E-4	6.159897068119992E-5	3.079948534059996E-5	8.310471132027384	0.0013089781269754982
84-85	1.0779819869209986E-4	6.159897068119992E-5	3.079948534059996E-5	9.02864583132816	0.0013089781269754982
86	1.0779819869209986E-4	7.69987133514999E-5	3.079948534059996E-5	9.583152065528985	0.0013089781269754982
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5685	0.0	40.87661	1
GTATCAA	9990	0.0	39.982334	1
TCAACGC	12515	0.0	31.133549	4
TATCAAC	12995	0.0	30.231533	2
CAACGCA	12860	0.0	30.190199	5
ATCAACG	13690	0.0	28.52895	3
AACGCAG	13860	0.0	28.283058	6
GTACATG	21240	0.0	26.53967	1
TACATGG	22465	0.0	24.768976	2
ACGCAGA	16575	0.0	22.841702	7
ACATGGG	23745	0.0	22.7952	3
CGCAGAG	17335	0.0	21.388422	8
CATGGGG	18060	0.0	19.997164	4
GAGTACA	16770	0.0	19.098097	1
AGTACAT	16020	0.0	18.327728	2
GCAGAGT	19450	0.0	17.385704	9
GTATAGG	1240	0.0	16.69955	1
AGAGTAC	15290	0.0	16.534405	10-11
GTATTAA	2045	0.0	15.976403	1
GTATAGA	1695	0.0	15.474588	1
>>END_MODULE