FastQCFastQC Report
Mon 15 Aug 2016
SRR2921577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6493615
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102700.041.4185261
GGTATCA60200.040.783151
TCAACGC133150.031.0660764
TATCAAC138050.030.031512
CAACGCA138250.029.8165425
ATCAACG145400.028.644723
AACGCAG148300.027.7602716
GTACATG215000.024.5868641
TACATGG228500.023.0693912
ACGCAGA182250.022.067937
CGCAGAG190050.020.9367148
ACATGGG243350.020.861993
CATGGGG182700.018.7415774
GAGTACA174200.018.354761
GCAGAGT206850.018.1778169
AGAGTAC167100.017.44451710-11
AGTACAT168250.017.3870122
GTATATA13850.016.4899391
GTGGTAT38350.015.7567241
TATACTG22550.015.5843215
Produced by FastQC (version 0.11.5)