##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921577_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6493615
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.099141079352563	31.0	30.0	34.0	26.0	34.0
2	30.091596129428677	31.0	30.0	34.0	26.0	34.0
3	30.266483923053645	31.0	30.0	34.0	26.0	34.0
4	33.92730520673	35.0	35.0	37.0	30.0	37.0
5	33.45693777656975	35.0	35.0	37.0	28.0	37.0
6	33.34933192066361	35.0	33.0	37.0	28.0	37.0
7	33.15644459981074	35.0	33.0	37.0	27.0	37.0
8	33.061791313467154	35.0	33.0	37.0	26.0	37.0
9	34.37886185121847	37.0	34.0	39.0	27.0	39.0
10-11	34.173098728520245	37.0	33.5	39.0	26.0	39.0
12-13	34.00093183842898	37.0	33.0	39.0	25.0	39.0
14-15	34.877686157864304	38.0	33.5	40.0	25.0	41.0
16-17	34.733815678940005	38.0	33.0	40.0	25.0	41.0
18-19	34.59546716274372	38.0	33.0	40.0	24.5	41.0
20-21	34.45479413239005	38.0	33.0	40.0	24.0	41.0
22-23	34.28651706329987	38.0	33.0	40.0	23.0	41.0
24-25	34.135732253914036	38.0	33.0	40.0	22.5	41.0
26-27	34.050614257235765	38.0	33.0	40.0	22.5	41.0
28-29	34.05732315513008	38.0	33.0	40.0	22.0	41.0
30-31	33.90130381921318	38.0	33.0	40.0	21.0	41.0
32-33	33.73463124931182	38.0	32.5	40.0	19.0	41.0
34-35	33.53398538410423	38.0	32.0	40.0	18.0	41.0
36-37	33.370290662443026	38.0	32.0	40.0	18.0	41.0
38-39	33.193029614475144	38.0	32.0	40.0	16.0	41.0
40-41	33.206667395587814	38.0	32.0	40.0	16.0	41.0
42-43	33.006775424782646	38.0	32.0	40.0	15.0	41.0
44-45	32.80848826116116	37.0	31.0	40.0	14.5	41.0
46-47	32.540195715329595	37.0	31.0	40.0	10.5	41.0
48-49	32.25013963716667	37.0	31.0	40.0	9.0	41.0
50-51	31.980687644709455	37.0	30.0	40.0	8.0	41.0
52-53	31.64865017405559	36.0	30.0	39.5	7.0	41.0
54-55	31.327421551785868	36.0	29.5	39.0	2.0	40.5
56-57	30.977018117027264	35.0	29.0	39.0	2.0	40.0
58-59	30.497203637727214	35.0	28.0	39.0	2.0	40.0
60-61	30.129847704244863	35.0	28.0	38.0	2.0	40.0
62-63	29.78914418240071	34.0	28.0	38.0	2.0	40.0
64-65	29.330497034394554	34.0	27.5	37.5	2.0	40.0
66-67	28.900018248695066	34.0	26.5	37.0	2.0	39.0
68-69	28.466571778585582	34.0	26.0	36.5	2.0	39.0
70-71	28.006279014077677	33.0	26.0	36.0	2.0	39.0
72-73	27.537022906347236	33.0	25.5	35.0	2.0	38.0
74-75	27.01081269831981	32.5	24.5	35.0	2.0	37.0
76-77	25.526757976874208	30.5	22.0	34.0	2.0	35.5
78-79	26.416631722083924	32.0	24.0	35.0	2.0	36.0
80-81	26.487703074481622	32.0	24.0	35.0	2.0	36.0
82-83	26.18716416048688	32.0	24.0	35.0	2.0	35.5
84-85	25.78090023815702	32.0	22.5	35.0	2.0	35.0
86-87	25.353556455071637	32.0	20.0	34.0	2.0	35.0
88-89	24.92355590838077	31.5	19.0	34.0	2.0	35.0
90-91	24.51514980484676	31.0	17.5	34.0	2.0	35.0
92-93	24.119895004554472	31.0	11.0	34.0	2.0	35.0
94-95	23.752524980307577	31.0	4.5	34.0	2.0	35.0
96-97	23.231573861400776	31.0	2.0	34.0	2.0	35.0
98-99	22.483506952598823	31.0	2.0	34.0	2.0	35.0
100-101	19.906918873385628	26.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	162098.0
3	74064.0
4	39152.0
5	28405.0
6	24383.0
7	24257.0
8	27785.0
9	31925.0
10	36301.0
11	41032.0
12	45292.0
13	49155.0
14	53137.0
15	56045.0
16	58506.0
17	60804.0
18	60050.0
19	58538.0
20	60037.0
21	62569.0
22	66760.0
23	75614.0
24	88473.0
25	103920.0
26	123209.0
27	145044.0
28	171177.0
29	201587.0
30	236800.0
31	280004.0
32	333142.0
33	404437.0
34	504397.0
35	642553.0
36	820823.0
37	856550.0
38	369706.0
39	15884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85593898400516	18.48845084369899	13.335503391835394	25.32010678046046
2	16.525232863358852	20.105057044496785	36.92616516378011	26.443544928364247
3	19.79833411666727	23.193794147232698	28.581242611970044	28.42662912412999
4	14.577212462027239	15.788613623804293	34.166444369558214	35.46772954461026
5	15.348736058197016	36.311127074328354	33.25508139597924	15.08505547149539
6	35.162748550371745	33.6755586038615	16.348328871100595	14.81336397466617
7	30.573371480685474	30.77037429300664	19.71633445541406	18.939919770893827
8	27.802402239737827	33.72179968708819	19.181981166065356	19.293816907108628
9	27.136121211093446	15.282874446834866	18.525036864828447	39.05596747724324
10-11	26.630914884377567	24.672931246044744	26.637244066122296	22.058909803455393
12-13	26.902605159327926	21.68589724766428	26.806442620359437	24.605054972648357
14-15	24.30431842127031	23.29313628198051	23.87980678464678	28.522738512102404
16-17	24.704747389209476	26.39934750463674	24.55494622769376	24.340958878460025
18-19	24.79024025778078	25.808924679446182	25.642510095376302	23.758324967396735
20-21	25.28503467148547	24.918378002048186	25.550869038631962	24.245718287834382
22-23	25.177398065129474	24.562481809006766	25.45794296306303	24.802177162800728
24-25	24.970096917349135	24.78131239761811	25.399408326198692	24.849182358834064
26-27	24.793021259824506	24.98658583474935	25.450629285166166	24.769763620259987
28-29	24.905927073757287	25.090218441104504	25.380664100449806	24.6231903846884
30-31	25.131960151846794	25.010340376702427	25.127746977875674	24.7299524935751
32-33	24.97736536028939	24.982587849901027	25.06311278559431	24.976934004215273
34-35	25.094513294951042	24.986197673351793	25.29372745398512	24.62556157771205
36-37	25.163845119616468	24.846934747335556	25.219142486425035	24.770077646622944
38-39	25.10070463007518	24.878155004135255	25.218865756404906	24.802274609384657
40-41	25.084884547234793	24.826774835435227	25.2720013807337	24.81633923659628
42-43	24.98766680810524	24.772836276752436	25.16715181714419	25.072345097998138
44-45	24.849709708181813	24.766044178387787	25.275130136325373	25.109115977105034
46-47	25.00197754266351	24.690415407194713	25.271485606300715	25.036121443841065
48-49	24.77259521956783	24.835672740393345	25.22624209663766	25.16548994340117
50-51	24.70497488617899	24.900684100468837	25.38307616255917	25.011264850792998
52-53	24.875094080827807	24.815199611525305	25.235531265790833	25.074175041856055
54-55	24.61561067143558	24.754946147566226	25.328922365273037	25.300520815725157
56-57	24.761820641407642	24.847813864676528	25.220425496655718	25.169939997260105
58-59	24.738181171344813	24.711121823279512	25.304409965294116	25.24628704008156
60-61	24.825796578835746	24.800340685349674	25.297512385389748	25.076350350424832
62-63	24.630032627838187	24.83548427762364	25.24316091048064	25.29132218405754
64-65	24.51726460712606	24.695080746162418	25.343751557046367	25.443903089665156
66-67	24.568163305054117	24.81495574677846	25.172127962878744	25.444752985288684
68-69	24.468984385198095	24.659640347431232	25.241317494191012	25.630057773179665
70-71	24.420474271444622	24.556313581766663	25.287732322307647	25.735479824481068
72-73	24.436375884437073	24.690687294883904	25.124919598380576	25.748017222298447
74-75	24.22237897850488	24.72405728672644	25.25715019081016	25.79641354395852
76-77	24.36315274543492	24.438677058454154	25.359458002533525	25.8387121935774
78-79	24.18130713612745	24.445353013703773	25.461953144281917	25.91138670588686
80-81	24.179188454026043	24.539368650399336	25.383154486961928	25.89828840861269
82-83	24.13656217368403	24.472842810806814	25.43108360773394	25.959511407775217
84-85	24.062665707034007	24.516696722577205	25.343089855427415	26.077547714961373
86-87	23.95846002857512	24.452890579658447	25.51916287625354	26.069486515512896
88-89	23.805580665465513	24.370499538128797	25.4587719667057	26.365147829699985
90-91	23.822166588992218	24.28584289748239	25.516450151071396	26.375540362453993
92-93	23.830521527162716	24.244681377577198	25.511158110309978	26.413638984950104
94-95	23.68532459842442	24.221977874746766	25.567957729940087	26.52473979688873
96-97	23.52884782866824	24.04189011103416	25.751825710082215	26.677436350215384
98-99	23.456308335148023	24.070195145825743	25.68161048514946	26.79188603387678
100-101	23.684503136161524	23.810102062314193	25.72655024696271	26.77884455456157
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	118.0
1	101.0
2	143.0
3	216.5
4	181.5
5	182.0
6	316.0
7	679.0
8	989.5
9	1001.5
10	863.5
11	730.5
12	741.0
13	778.5
14	858.0
15	973.0
16	1064.5
17	1153.0
18	1281.0
19	1404.0
20	1513.5
21	1682.5
22	1918.5
23	2173.5
24	2390.0
25	2738.0
26	3388.0
27	4389.5
28	5758.0
29	8100.0
30	11814.0
31	16891.5
32	24030.0
33	32132.0
34	41375.5
35	53079.5
36	67112.5
37	82133.5
38	98066.0
39	115311.5
40	133182.5
41	150497.5
42	167261.0
43	186211.0
44	207229.0
45	226982.0
46	247340.5
47	267502.0
48	286115.0
49	301643.0
50	310344.0
51	317477.5
52	322943.0
53	325470.5
54	324303.5
55	316022.5
56	298836.0
57	273016.0
58	246105.0
59	216703.0
60	183521.0
61	151753.0
62	121573.5
63	92018.5
64	65670.0
65	46849.5
66	34202.5
67	24785.5
68	17534.5
69	12457.0
70	8832.5
71	6213.5
72	4361.0
73	3134.0
74	2270.0
75	1468.5
76	883.5
77	528.0
78	305.5
79	144.0
80	86.0
81	51.0
82	25.5
83	12.5
84	7.0
85	8.5
86	8.0
87	4.5
88	1.0
89	1.5
90	3.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2237120617714478
2	0.0
3	4.619922801089994E-5
4	0.012365993364250882
5	0.01402916557264328
6	0.10705901104392544
7	0.13761210050180062
8	0.15398202696032948
9	0.10903017810572385
10-11	0.1198792968169502
12-13	0.09488551446305332
14-15	0.1184471207486123
16-17	0.0720707956970039
18-19	0.0770141130941702
20-21	0.05364500359198998
22-23	0.07962436947678604
24-25	0.07292548141520555
26-27	0.05042645737389728
28-29	0.018148596736948526
30-31	0.03190826681286156
32-33	0.03785256748359735
34-35	0.04406636365106339
36-37	0.036150895918529206
38-39	0.038168262208338494
40-41	0.02178293600713932
42-43	0.03059158881455091
44-45	0.016885817837983926
46-47	0.030375992417166712
48-49	2.771953680653996E-4
50-51	0.016547023499237326
52-53	0.008131064129918389
54-55	0.01557683971100843
56-57	0.010186929776403435
58-59	0.00739187648174399
60-61	0.030714786755913312
62-63	0.013035882170408934
64-65	0.014683654636131029
66-67	0.057725935399619475
68-69	0.0764443226153691
70-71	0.03267055407504141
72-73	0.054522788924197076
74-75	0.04622232762490539
76-77	0.19897237517161087
78-79	0.07361846983536904
80-81	0.01588483456441443
82-83	0.010394826302452486
84-85	0.023831101782289216
86-87	0.16481574592888554
88-89	0.3738903522922132
90-91	0.2751780017755903
92-93	0.27495470550687096
94-95	0.0020019665471389973
96-97	0.005975100156076392
98-99	0.004881718426485093
100-101	0.005035715853188094
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6493615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.86975371512534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.76772711372394	34.83373175343413
2	12.853966172229958	12.306323898544012
3	5.314595737700537	7.632251671775387
4	2.6453321746525194	5.065255987812522
5	1.591733441505243	3.809794391249243
6	1.0155627626689911	2.916884359873014
7	0.6833603651626029	2.2898604665308344
8	0.5036957579905553	1.9289433505889009
9	0.3627256481365124	1.5627228698208655
>10	2.0544616480157143	18.16267227671983
>50	0.15083251810918163	4.956936123933735
>100	0.05542573744585056	4.247245476665302
>500	3.872945284963713E-4	0.10375985228451073
>1k	1.93628129776347E-4	0.18361752076777954
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.079948534059996E-5
2	0.0	0.0	0.0	0.0	4.619922801089994E-5
3	0.0	0.0	0.0	0.0	4.619922801089994E-5
4	0.0	0.0	0.0	1.539974267029998E-5	9.239845602179988E-5
5	0.0	0.0	0.0	1.539974267029998E-5	9.239845602179988E-5
6	0.0	0.0	0.0	1.539974267029998E-5	1.539974267029998E-4
7	0.0	0.0	0.0	1.539974267029998E-5	1.539974267029998E-4
8	0.0	0.0	0.0	1.539974267029998E-5	1.8479691204359975E-4
9	0.0	0.0	0.0	4.619922801089994E-5	2.0019665471389972E-4
10-11	0.0	0.0	0.0	8.46985846866499E-5	2.0019665471389972E-4
12-13	0.0	0.0	0.0	1.0779819869209986E-4	2.078965260490497E-4
14-15	7.69987133514999E-6	0.0	0.0	1.0779819869209986E-4	2.6179562539509964E-4
16-17	1.539974267029998E-5	0.0	0.0	1.0779819869209986E-4	3.4649421008174956E-4
18-19	1.539974267029998E-5	0.0	0.0	1.1549807002724985E-4	3.695938240871995E-4
20-21	1.539974267029998E-5	0.0	0.0	1.4629755536784978E-4	3.9269343809264945E-4
22-23	1.539974267029998E-5	0.0	0.0	2.6179562539509964E-4	4.8509189411444936E-4
24-25	1.539974267029998E-5	0.0	0.0	5.235912507901993E-4	6.005899641416992E-4
26-27	1.539974267029998E-5	0.0	0.0	0.0011395809576021986	6.159897068119992E-4
28-29	1.539974267029998E-5	0.0	0.0	0.004650722286430594	6.159897068119992E-4
30-31	3.079948534059996E-5	0.0	0.0	0.018009999052915827	6.236895781471492E-4
32-33	3.849935667574995E-5	0.0	0.0	0.04697691501575009	6.621889348228991E-4
34-35	4.619922801089994E-5	0.0	0.0	0.08630785779569623	6.77588677493199E-4
36-37	4.619922801089994E-5	0.0	0.0	0.15203395951253654	6.852885488283491E-4
38-39	4.619922801089994E-5	0.0	0.0	0.2576453947454538	7.23787905504099E-4
40-41	4.619922801089994E-5	0.0	0.0	0.3763466112481261	7.39187648174399E-4
42-43	4.619922801089994E-5	0.0	7.69987133514999E-6	0.5004839369134142	7.39187648174399E-4
44-45	4.619922801089994E-5	0.0	1.539974267029998E-5	0.6534264812434984	7.39187648174399E-4
46-47	4.619922801089994E-5	0.0	1.539974267029998E-5	0.833903765468079	7.62287262179849E-4
48-49	4.619922801089994E-5	0.0	1.539974267029998E-5	1.025284067503232	8.007866188555989E-4
50-51	4.619922801089994E-5	0.0	1.539974267029998E-5	1.2283835737104833	8.007866188555989E-4
52-53	4.619922801089994E-5	0.0	1.539974267029998E-5	1.4404765296371898	8.392859755313488E-4
54-55	4.619922801089994E-5	0.0	1.539974267029998E-5	1.6791263417988285	8.777853322070988E-4
56-57	4.619922801089994E-5	0.0	1.539974267029998E-5	1.9583313762827022	8.777853322070988E-4
58-59	4.619922801089994E-5	0.0	1.539974267029998E-5	2.257956469547394	8.931850748773988E-4
60-61	4.619922801089994E-5	7.69987133514999E-6	1.539974267029998E-5	2.5766387443665817	9.393843028882988E-4
62-63	4.619922801089994E-5	1.539974267029998E-5	1.539974267029998E-5	2.9079103088187397	9.701837882288987E-4
64-65	4.619922801089994E-5	1.539974267029998E-5	1.539974267029998E-5	3.267440401070898	9.778836595640487E-4
66-67	4.619922801089994E-5	1.539974267029998E-5	1.539974267029998E-5	3.675479682734501	9.932834022343487E-4
68-69	4.619922801089994E-5	1.539974267029998E-5	1.539974267029998E-5	4.131920355610857	0.0010086831449046486
70-71	5.389909934604993E-5	3.079948534059996E-5	1.539974267029998E-5	4.616958350625961	0.0010471825015803985
72-73	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	5.1186280677249885	0.0011934800569482484
74-75	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	5.645322366663253	0.0012011799282833984
76-77	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	6.228402823388821	0.0012088797996185484
78-79	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	6.863187916129922	0.0012165796709536984
80-81	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	7.5409228911784885	0.0012473791562942983
82-83	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	8.238885428224494	0.0013320777409809481
84-85	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	8.955966745795678	0.0013397776123160981
86-87	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	9.707104594282228	0.0013397776123160981
88-89	6.159897068119992E-5	3.079948534059996E-5	1.539974267029998E-5	10.526032110003442	0.001362877226321548
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10270	0.0	41.418526	1
GGTATCA	6020	0.0	40.78315	1
TCAACGC	13315	0.0	31.066076	4
TATCAAC	13805	0.0	30.03151	2
CAACGCA	13825	0.0	29.816542	5
ATCAACG	14540	0.0	28.64472	3
AACGCAG	14830	0.0	27.760271	6
GTACATG	21500	0.0	24.586864	1
TACATGG	22850	0.0	23.069391	2
ACGCAGA	18225	0.0	22.06793	7
CGCAGAG	19005	0.0	20.936714	8
ACATGGG	24335	0.0	20.86199	3
CATGGGG	18270	0.0	18.741577	4
GAGTACA	17420	0.0	18.35476	1
GCAGAGT	20685	0.0	18.177816	9
AGAGTAC	16710	0.0	17.444517	10-11
AGTACAT	16825	0.0	17.387012	2
GTATATA	1385	0.0	16.489939	1
GTGGTAT	3835	0.0	15.756724	1
TATACTG	2255	0.0	15.584321	5
>>END_MODULE