FastQCFastQC Report
Mon 15 Aug 2016
SRR2921576_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921576_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5834182
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC73260.12557030274338374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.047.1719251
GTATCAA132050.044.4286351
TCAACGC176300.033.072164
TATCAAC177250.032.9470332
CAACGCA180200.032.2280735
ATCAACG181300.032.135013
GTACATG323150.030.8564531
AACGCAG189700.030.8266856
TACATGG341000.029.02342
ACATGGG353450.027.3120983
CATGGGG293600.026.0249214
ACGCAGA223950.025.7591047
ATGGGGG212600.025.6308785
CGCAGAG229250.024.8211368
TGGGGGG193950.024.7078786
GTAAGTA64500.022.5104582
GCAGAGT247950.021.873869
TGTAAGT65750.021.4149421
ATATGTA39600.021.1479071
AGTACAT224850.019.9849432
Produced by FastQC (version 0.11.5)