##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921576_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5834182
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.467558434070106	31.0	28.0	31.0	20.0	34.0
2	28.439756421722873	31.0	28.0	33.0	16.0	34.0
3	28.52338219822419	31.0	28.0	34.0	16.0	34.0
4	31.98759226229144	35.0	32.0	37.0	22.0	37.0
5	31.929978701384357	35.0	32.0	37.0	22.0	37.0
6	32.030597262821075	35.0	32.0	37.0	22.0	37.0
7	31.844888452228606	35.0	32.0	37.0	19.0	37.0
8	31.803092018726876	35.0	32.0	37.0	19.0	37.0
9	33.051611005621695	37.0	32.0	39.0	19.0	39.0
10-11	32.81811529019835	37.0	32.0	39.0	17.5	39.0
12-13	32.60928275806275	37.0	32.0	39.0	17.0	39.0
14-15	33.319195218798455	38.0	32.0	40.0	17.0	41.0
16-17	33.1665775939112	38.0	32.0	40.0	16.5	41.0
18-19	32.99854983954906	38.0	32.0	40.0	15.0	41.0
20-21	32.875310454833254	38.0	32.0	40.0	11.0	41.0
22-23	32.67958130205743	37.5	31.0	40.0	10.0	41.0
24-25	32.777219497094876	38.0	32.0	40.0	10.0	41.0
26-27	32.53673034197425	37.5	31.0	40.0	10.0	41.0
28-29	32.345634229442965	37.0	31.0	40.0	9.0	41.0
30-31	32.086410314248	37.0	30.0	40.0	8.0	41.0
32-33	31.831368476334816	37.0	30.0	40.0	7.5	41.0
34-35	31.505014241928002	37.0	30.0	40.0	2.0	41.0
36-37	31.226132815191573	36.0	30.0	40.0	2.0	41.0
38-39	31.015045382540347	36.0	29.5	39.5	2.0	41.0
40-41	30.795209165569396	36.0	29.0	39.0	2.0	40.5
42-43	30.55751243276264	36.0	28.5	39.0	2.0	40.0
44-45	30.309919796811275	35.0	28.0	39.0	2.0	40.0
46-47	30.008180838376312	35.0	27.0	39.0	2.0	40.0
48-49	29.726566466387233	35.0	27.0	39.0	2.0	40.0
50-51	28.796848812738446	34.0	25.0	38.0	2.0	39.5
52-53	29.007939416356912	34.5	26.5	38.0	2.0	39.5
54-55	29.606403262702464	35.0	27.0	39.0	2.0	40.0
56-57	29.605105908591813	35.0	27.0	39.0	2.0	40.0
58-59	29.37156305716894	35.0	27.0	39.0	2.0	40.0
60-61	29.01537576304613	34.5	26.0	38.5	2.0	40.0
62-63	28.56785209991735	34.0	26.0	38.0	2.0	40.0
64-65	28.120048363249552	34.0	25.5	37.5	2.0	40.0
66-67	27.78776793387659	34.0	25.0	37.0	2.0	39.5
68-69	27.33346517129565	33.5	23.5	36.5	2.0	39.0
70-71	26.901265421613516	33.0	22.5	36.0	2.0	39.0
72-73	26.414622135545308	32.5	21.5	35.5	2.0	38.5
74-75	25.96616860769856	32.0	20.0	35.0	2.0	37.5
76-77	25.536729484956076	32.0	18.5	35.0	2.0	37.0
78-79	25.138886222610125	32.0	18.0	35.0	2.0	36.5
80-81	24.705830054667473	31.5	13.0	35.0	2.0	36.0
82-83	24.15747588950773	31.0	7.0	34.0	2.0	35.5
84-85	23.742346742011136	31.0	3.5	34.0	2.0	35.0
86-87	23.247484308854265	30.5	2.0	34.0	2.0	35.0
88-89	22.748599546603106	30.0	2.0	34.0	2.0	35.0
90-91	22.149785436930145	30.0	2.0	34.0	2.0	35.0
92-93	21.58449522143807	29.0	2.0	34.0	2.0	35.0
94-95	20.88959137716307	29.0	2.0	34.0	2.0	35.0
96-97	20.247073540043832	29.0	2.0	34.0	2.0	35.0
98	19.774929887343248	27.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	317437.0
3	56017.0
4	42182.0
5	33294.0
6	30669.0
7	32274.0
8	36288.0
9	41363.0
10	43161.0
11	45630.0
12	46771.0
13	46335.0
14	45424.0
15	47832.0
16	51695.0
17	56009.0
18	58094.0
19	58265.0
20	60659.0
21	64584.0
22	70509.0
23	78657.0
24	89494.0
25	101508.0
26	117288.0
27	134743.0
28	155654.0
29	179486.0
30	207516.0
31	243650.0
32	287793.0
33	350066.0
34	432413.0
35	516809.0
36	635468.0
37	662432.0
38	330749.0
39	25963.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.80987992280494	17.52444398537288	13.163170552170858	24.502505539651324
2	16.219234612457637	19.753128586027064	39.67848944997918	24.349147351536118
3	19.646587897611784	24.6853851726462	28.861891106069454	26.806135823672562
4	14.128545668569863	15.193372488764536	35.64078990702201	35.03729193564359
5	14.75274206195096	38.18308717571523	32.34214727652365	14.722023485810162
6	33.49563460230588	33.20118418078786	18.205339171011715	15.097842045894549
7	29.583948240307272	32.48689212941363	20.166051030977595	17.763108599301496
8	28.235333041451856	33.95167293000433	19.925299369940575	17.88769465860324
9	29.76222678656465	15.189866474127756	18.48565540540309	36.562251333904506
10-11	27.055064280469853	24.95252251789763	26.54879778419276	21.443615417439744
12-13	27.015643751078837	21.9745738263587	26.96514520218361	24.044637220378856
14-15	24.911938097262922	23.475957174191958	24.50903512507889	27.103069603466235
16-17	24.77249370248581	26.2077209910883	25.13087627646205	23.888909029963834
18-19	24.763460955409556	26.27112191548353	26.31584543223358	22.649571696873334
20-21	25.36778456337657	25.2491128357619	26.216192197072274	23.16691040378926
22-23	25.392044514361988	24.54067285716602	25.903078530884	24.16420409758799
24-25	24.830975297874037	24.604451236821546	25.790938866033226	24.77363459927119
26-27	24.31068162685389	25.118867959322223	26.10037186040418	24.470078553419714
28-29	24.295184446313627	26.25697279254417	25.359411130646485	24.08843163049572
30-31	25.86149123399098	25.218681733652502	25.378950994931028	23.54087603742549
32-33	25.275099983173632	25.197054091160513	25.307698393643253	24.2201475320226
34-35	25.191322200182526	25.617148286287012	25.730809634260403	23.46071987927006
36-37	24.952690902252755	25.722849808174274	25.837408450723608	23.487050838849356
38-39	25.156526359260784	25.743965687612587	25.341084787311463	23.75842316581517
40-41	25.28159955928221	25.271993686439025	25.226011052515574	24.220395701763188
42-43	24.672808455243565	25.410524655982776	25.026500230289127	24.89016665848453
44-45	24.15455835055602	25.442266658207906	25.805142202547017	24.598032788689057
46-47	24.22723174433751	24.867619023384467	25.59982792794156	25.305321304336463
48-49	24.153779130090637	25.342415942682045	25.818489592690636	24.685315334536682
50-51	24.507529943118175	25.086930246911987	26.047061392310937	24.358478417658898
52-53	24.447035436707043	25.146973161729264	25.837183915356256	24.56880748620744
54-55	23.366414758032533	24.771976881412154	26.659116057636183	25.202492302919126
56-57	24.07061682030859	24.83167310638693	25.86152108084127	25.236188992463205
58-59	24.27347425316589	25.21781094509401	26.0589940513906	24.449720750349496
60-61	25.211625535656516	24.919119057315022	26.121305638880145	23.74794976814832
62-63	24.679213874009083	24.764192893174968	26.28648219786262	24.270111034953327
64-65	24.0988442128357	24.42101874562244	26.500209538120203	24.979927503421653
66-67	24.367109563408242	24.745184876093088	25.964697490729577	24.923008069769093
68-69	23.701619177387396	24.869868342438608	26.30972838911837	25.118784091055623
70-71	23.846688038779213	24.539436704454623	26.354315820233605	25.25955943653256
72-73	23.569320256329082	25.401102835149793	25.859110781585088	25.170466126936038
74-75	23.4978423597246	24.972537053818954	26.285714825797925	25.243905760658524
76-77	23.28448940893908	24.21526613371241	26.29265522955705	26.207589227791455
78-79	23.16549245305523	24.426412948991914	26.78504586805934	25.623048729893515
80-81	23.698677818015224	24.633710798886412	26.112237123738435	25.55537425935993
82-83	23.68976841083506	24.730058401702383	26.320611975322176	25.259561212140376
84-85	23.619752512739627	24.900572207098516	25.90553917162037	25.574136108541484
86-87	22.953706613079543	25.204463726231392	26.241519933167318	25.600309727521747
88-89	22.86111551316899	24.393845224103323	26.712654516528573	26.032384746199117
90-91	23.716699367460052	23.764844032080862	26.833428396776654	25.685028203682435
92-93	24.004192133614893	23.732397669822568	26.479176543709897	25.784233652852638
94-95	23.662296723393265	24.04314362998317	26.396849694884228	25.89770995173934
96-97	22.860220143053283	23.85590283416952	26.847952049531585	26.435924973245612
98	22.924938818159323	23.48992561938021	26.460200868919625	27.124934693540848
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	169.0
1	187.0
2	302.0
3	427.5
4	456.0
5	543.0
6	799.5
7	1365.5
8	1970.0
9	2189.5
10	2111.5
11	2086.0
12	2348.5
13	2702.0
14	3037.5
15	3318.0
16	3498.0
17	3626.0
18	3751.5
19	3748.0
20	3708.0
21	3732.0
22	3812.0
23	4099.0
24	4585.5
25	5543.0
26	7181.0
27	9815.5
28	13749.5
29	19265.0
30	26360.5
31	34843.0
32	44552.0
33	54630.0
34	65293.5
35	77532.0
36	90364.5
37	102313.5
38	113678.0
39	124094.5
40	133911.0
41	144740.0
42	158482.0
43	175969.5
44	194951.5
45	215060.0
46	237405.0
47	259352.0
48	278062.0
49	294535.0
50	302523.0
51	301106.0
52	299952.0
53	297753.5
54	289560.5
55	274667.0
56	253913.0
57	230514.0
58	200313.0
59	165333.0
60	135279.5
61	109704.5
62	87552.5
63	66954.0
64	48432.5
65	35272.5
66	26291.0
67	19601.0
68	14491.0
69	10532.0
70	7536.5
71	5439.0
72	4075.5
73	3067.0
74	2239.5
75	1624.5
76	1078.0
77	647.0
78	368.5
79	205.5
80	129.0
81	83.0
82	52.0
83	44.5
84	36.5
85	24.5
86	18.5
87	16.0
88	14.5
89	14.0
90	12.0
91	12.0
92	12.0
93	10.0
94	9.0
95	5.5
96	3.0
97	5.0
98	5.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.268675197311294
2	0.13280353612554424
3	0.13048958705779148
4	0.12545032019912988
5	0.12150803660221775
6	0.04525055954716531
7	0.03426358656620585
8	0.013095237687134205
9	0.009530042086448452
10-11	0.001722606528215952
12-13	0.006016267576157206
14-15	0.011029823889621544
16-17	0.0056991708520577515
18-19	0.04795873697460929
20-21	0.09423771832966472
22-23	0.09275507688995646
24-25	0.07056687638472711
26-27	0.08072254173764205
28-29	0.09812001065444993
30-31	0.07379783489784858
32-33	0.018434460906430413
34-35	0.28968928291918217
36-37	0.3749454507932732
38-39	0.34803508015348167
40-41	0.34333005038238434
42-43	0.31995059461634895
44-45	0.3038129424142065
46-47	0.3087665074555439
48-49	0.30244171333701964
50-51	0.3040186267757845
52-53	0.3505290030376152
54-55	0.33663673844936615
56-57	0.32073048115399894
58-59	0.3018846515244125
60-61	0.3097520783547719
62-63	0.30922072708736204
64-65	0.32585544982998477
66-67	0.3472809041610289
68-69	0.3077895067380483
70-71	0.2850785251471415
72-73	0.2695150751210709
74-75	0.2567197937945713
76-77	0.260036454125017
78-79	0.2662926867896819
80-81	0.2566855130676417
82-83	0.26594987952038524
84-85	0.25941940104028294
86-87	0.2549371959942285
88-89	0.27760532667647325
90-91	0.26157908683685216
92-93	0.2521518869311927
94-95	0.2668925995109512
96-97	0.26586417770306103
98	0.26502429989328413
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5834182.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.98538321885238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86090740010837	40.47629108962212
2	11.345576012928017	11.796082337413642
3	4.055477913649892	6.324767204300446
4	1.9661758702206569	4.088496243563256
5	1.1282377214419237	2.932593515556161
6	0.6840140190883737	2.1335238545625903
7	0.4845260458216035	1.76317905200859
8	0.36689778767162656	1.5258657675406908
9	0.2659918877516993	1.2444921196090246
>10	1.6486659058715074	16.142369071396033
>50	0.1347245708845648	4.795237256109818
>100	0.05594743455997462	5.02345444728968
>500	0.0018606596930658254	0.6459629050433765
>1k	9.303189548397801E-4	0.8908980450563199
>5k	6.64513539120536E-5	0.21678709092823076
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7326	0.12557030274338374	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7140363464835345E-5
2	0.0	0.0	0.0	0.0	1.7140363464835345E-5
3	0.0	0.0	0.0	0.0	1.7140363464835345E-5
4	0.0	0.0	0.0	0.0	1.7140363464835345E-5
5	0.0	0.0	0.0	1.7140363464835345E-5	1.7140363464835345E-5
6	0.0	0.0	0.0	1.7140363464835345E-5	1.7140363464835345E-5
7	0.0	0.0	0.0	1.7140363464835345E-5	1.7140363464835345E-5
8	0.0	0.0	0.0	1.7140363464835345E-5	3.428072692967069E-5
9	0.0	0.0	0.0	3.428072692967069E-5	3.428072692967069E-5
10-11	0.0	0.0	0.0	3.428072692967069E-5	3.428072692967069E-5
12-13	0.0	0.0	0.0	3.428072692967069E-5	3.428072692967069E-5
14-15	0.0	0.0	0.0	3.428072692967069E-5	7.713163559175905E-5
16-17	8.570181732417673E-6	0.0	0.0	3.428072692967069E-5	1.3712290771868276E-4
18-19	2.5710545197253018E-5	0.0	0.0	4.285090866208836E-5	1.7140363464835344E-4
20-21	3.428072692967069E-5	0.0	0.0	6.856145385934138E-5	1.7140363464835344E-4
22-23	3.428072692967069E-5	8.570181732417673E-6	0.0	2.5710545197253013E-4	1.8854399811318878E-4
24-25	3.428072692967069E-5	1.7140363464835345E-5	0.0	8.827287184390202E-4	2.2282472504285948E-4
26-27	5.1421090394506036E-5	1.7140363464835345E-5	0.0	0.0017397468916807873	2.2282472504285948E-4
28-29	5.1421090394506036E-5	1.7140363464835345E-5	0.0	0.0058620043049736874	2.571054519725302E-4
30-31	5.1421090394506036E-5	1.7140363464835345E-5	0.0	0.022565288501455734	2.6567563370494783E-4
32-33	5.1421090394506036E-5	1.7140363464835345E-5	0.0	0.05916853468061161	2.9138617890220087E-4
34-35	5.1421090394506036E-5	1.7140363464835345E-5	0.0	0.10660449056954344	3.085265423670362E-4
36-37	5.1421090394506036E-5	3.428072692967069E-5	0.0	0.18263914289955302	3.085265423670362E-4
38-39	5.1421090394506036E-5	4.285090866208836E-5	0.0	0.31414858158350223	3.170967240994539E-4
40-41	5.1421090394506036E-5	5.1421090394506036E-5	0.0	0.47147997782722584	3.2566690583187154E-4
42-43	5.1421090394506036E-5	5.1421090394506036E-5	0.0	0.6247748184749807	3.2566690583187154E-4
44-45	5.1421090394506036E-5	5.1421090394506036E-5	0.0	0.8036773621391997	3.2566690583187154E-4
46-47	5.1421090394506036E-5	5.1421090394506036E-5	0.0	1.0033026052324043	3.2566690583187154E-4
48-49	5.1421090394506036E-5	5.1421090394506036E-5	0.0	1.2222021870418167	3.513774510291246E-4
50-51	5.1421090394506036E-5	5.1421090394506036E-5	0.0	1.4670094282283275	3.599476327615422E-4
52-53	5.1421090394506036E-5	5.1421090394506036E-5	0.0	1.7269087594456258	3.9422835969121295E-4
54-55	5.1421090394506036E-5	6.856145385934138E-5	0.0	2.0200861063299023	4.113687231560483E-4
56-57	5.1421090394506036E-5	6.856145385934138E-5	0.0	2.331466519213833	4.542196318181366E-4
58-59	5.1421090394506036E-5	6.856145385934138E-5	0.0	2.6807528459002476	4.627898135505543E-4
60-61	5.1421090394506036E-5	6.856145385934138E-5	0.0	3.063522872615218	4.627898135505543E-4
62-63	5.1421090394506036E-5	6.856145385934138E-5	0.0	3.476022859759946	4.627898135505543E-4
64-65	5.1421090394506036E-5	6.856145385934138E-5	0.0	3.8951647377472973	4.71359995282972E-4
66-67	5.1421090394506036E-5	8.570181732417672E-5	0.0	4.367801690108399	4.7993017701538965E-4
68-69	5.1421090394506036E-5	8.570181732417672E-5	0.0	4.890814170692652	4.7993017701538965E-4
70-71	6.856145385934138E-5	8.570181732417672E-5	0.0	5.469549972901086	5.142109039450604E-4
72-73	6.856145385934138E-5	8.570181732417672E-5	0.0	6.048765705286534	5.48491630874731E-4
74-75	6.856145385934138E-5	1.0284218078901207E-4	0.0	6.637374356850712	5.74202176071984E-4
76-77	6.856145385934138E-5	1.0284218078901207E-4	0.0	7.28198914603624	5.913425395368194E-4
78-79	6.856145385934138E-5	1.0284218078901207E-4	8.570181732417673E-6	7.992757168014299	5.99912721269237E-4
80-81	6.856145385934138E-5	1.0284218078901207E-4	1.7140363464835345E-5	8.722276747622889	6.427636299313254E-4
82-83	6.856145385934138E-5	1.0284218078901207E-4	1.7140363464835345E-5	9.485837089072643	7.198952655230844E-4
84-85	6.856145385934138E-5	1.0284218078901207E-4	1.7140363464835345E-5	10.26920312050601	7.284654472555021E-4
86	6.856145385934138E-5	1.1998254425384741E-4	1.7140363464835345E-5	10.870709895577479	7.370356289879198E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6955	0.0	47.171925	1
GTATCAA	13205	0.0	44.428635	1
TCAACGC	17630	0.0	33.07216	4
TATCAAC	17725	0.0	32.947033	2
CAACGCA	18020	0.0	32.228073	5
ATCAACG	18130	0.0	32.13501	3
GTACATG	32315	0.0	30.856453	1
AACGCAG	18970	0.0	30.826685	6
TACATGG	34100	0.0	29.0234	2
ACATGGG	35345	0.0	27.312098	3
CATGGGG	29360	0.0	26.024921	4
ACGCAGA	22395	0.0	25.759104	7
ATGGGGG	21260	0.0	25.630878	5
CGCAGAG	22925	0.0	24.821136	8
TGGGGGG	19395	0.0	24.707878	6
GTAAGTA	6450	0.0	22.510458	2
GCAGAGT	24795	0.0	21.87386	9
TGTAAGT	6575	0.0	21.414942	1
ATATGTA	3960	0.0	21.147907	1
AGTACAT	22485	0.0	19.984943	2
>>END_MODULE