FastQCFastQC Report
Mon 15 Aug 2016
SRR2921576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5834182
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC73750.12641018055316067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70000.047.7206841
GTATCAA127050.044.41991
TCAACGC176600.031.4915434
TATCAAC177700.031.4291572
CAACGCA182300.030.4545275
ATCAACG183800.030.3354423
GTACATG326800.029.7477341
AACGCAG191650.029.1139326
TACATGG339050.028.3784432
ACATGGG353200.026.7987383
CATGGGG288100.025.9636084
ATGGGGG207500.025.1995755
ATATGTA40650.024.6995741
TGGGGGG183500.024.6878996
GTAAGTA67000.023.5329362
ACGCAGA233600.023.4180897
CGCAGAG238450.022.722328
TATGTAA45450.022.1520962
TGTAAGT69750.022.103871
GAGTACA248850.020.5177441
Produced by FastQC (version 0.11.5)