##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921576_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5834182
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.07062446800597	31.0	30.0	34.0	26.0	34.0
2	30.05604710309003	31.0	30.0	34.0	26.0	34.0
3	30.2266315997684	31.0	30.0	34.0	26.0	34.0
4	33.89449283549948	35.0	35.0	37.0	30.0	37.0
5	33.417547995588755	35.0	35.0	37.0	28.0	37.0
6	33.31238226712845	35.0	33.0	37.0	28.0	37.0
7	33.12850404735403	35.0	33.0	37.0	27.0	37.0
8	33.02635793672532	35.0	33.0	37.0	26.0	37.0
9	34.32445028283314	37.0	34.0	39.0	27.0	39.0
10-11	34.11140336383061	37.0	33.5	39.0	26.0	39.0
12-13	33.91349421392751	37.0	33.0	39.0	25.0	39.0
14-15	34.74753315889014	38.0	33.0	40.0	25.0	41.0
16-17	34.593429464490484	38.0	33.0	40.0	25.0	41.0
18-19	34.47572804550835	38.0	33.0	40.0	24.0	41.0
20-21	34.34772475387295	38.0	33.0	40.0	23.0	41.0
22-23	34.20172296647585	38.0	33.0	40.0	22.5	41.0
24-25	34.03924963259631	38.0	33.0	40.0	22.0	41.0
26-27	33.950068407190585	38.0	33.0	40.0	21.5	41.0
28-29	33.94761287872062	38.0	33.0	40.0	21.0	41.0
30-31	33.774758826515864	38.0	32.5	40.0	19.0	41.0
32-33	33.5847530982064	38.0	32.0	40.0	18.5	41.0
34-35	33.38999820368991	38.0	32.0	40.0	18.0	41.0
36-37	33.23644737171381	38.0	32.0	40.0	16.5	41.0
38-39	33.06650255339994	37.5	32.0	40.0	16.0	41.0
40-41	33.07991668754934	38.0	32.0	40.0	15.5	41.0
42-43	32.881329293463935	37.5	31.5	40.0	15.0	41.0
44-45	32.68557494435381	37.0	31.0	40.0	12.5	41.0
46-47	32.41567335403661	37.0	31.0	40.0	9.0	41.0
48-49	32.14516516625638	37.0	31.0	40.0	8.0	41.0
50-51	31.892943946554976	36.5	30.0	40.0	7.5	41.0
52-53	31.586769661968034	36.0	30.0	39.5	4.0	41.0
54-55	31.28037041011062	36.0	30.0	39.0	2.0	41.0
56-57	30.966573634487233	35.0	29.0	39.0	2.0	40.0
58-59	30.50692873482521	35.0	28.0	39.0	2.0	40.0
60-61	30.14594419577586	35.0	28.0	38.5	2.0	40.0
62-63	29.790349015508944	34.5	28.0	38.0	2.0	40.0
64-65	29.36824399718761	34.0	27.5	38.0	2.0	40.0
66-67	28.977578347744377	34.0	26.5	37.0	2.0	39.5
68-69	28.561255802441543	34.0	26.0	36.5	2.0	39.0
70-71	28.109437792650283	33.5	26.0	36.0	2.0	39.0
72-73	27.635541623487235	33.0	26.0	35.5	2.0	38.5
74-75	27.11150012118237	32.5	25.0	35.0	2.0	37.5
76-77	25.621222221041442	30.5	22.5	34.0	2.0	36.0
78-79	26.518191581956135	32.0	24.5	35.0	2.0	36.0
80-81	26.58849329691806	33.0	25.0	35.0	2.0	36.0
82-83	26.275494662319414	32.0	24.0	35.0	2.0	36.0
84-85	25.86870644419389	32.0	23.0	35.0	2.0	35.0
86-87	25.4469068671495	32.0	20.0	34.5	2.0	35.0
88-89	25.03531566207568	32.0	19.0	34.0	2.0	35.0
90-91	24.62402732722428	31.0	17.5	34.0	2.0	35.0
92-93	24.21632552772608	31.0	13.0	34.0	2.0	35.0
94-95	23.845694563522358	31.0	4.5	34.0	2.0	35.0
96-97	23.342721910286652	31.0	2.0	34.0	2.0	35.0
98-99	22.635352308172763	31.0	2.0	34.0	2.0	35.0
100-101	20.068106205805712	27.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	151143.0
3	69317.0
4	37615.0
5	26031.0
6	22288.0
7	22171.0
8	24538.0
9	28387.0
10	31946.0
11	35653.0
12	39469.0
13	43341.0
14	46959.0
15	50213.0
16	53513.0
17	55799.0
18	54398.0
19	51869.0
20	52804.0
21	54906.0
22	59513.0
23	67459.0
24	78250.0
25	92431.0
26	109226.0
27	129667.0
28	152332.0
29	179622.0
30	210755.0
31	250222.0
32	299328.0
33	365680.0
34	457682.0
35	559972.0
36	715380.0
37	776435.0
38	358779.0
39	19089.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.91718002403646	18.695375969996338	13.812479857952612	24.574964148014587
2	16.078552228915726	20.761042422056768	38.432277224124995	24.728128124902515
3	19.427072674162496	25.238174083821235	29.19508112368186	26.139672118334406
4	14.717819560446568	16.00616168843703	35.047328637897266	34.22869011321914
5	14.80877800770089	38.312232540095735	32.35603343601302	14.52295601619035
6	33.6423559247728	33.65381794097233	17.86887144611008	14.83495468814479
7	29.480525387582762	32.53855230919085	20.251245456924998	17.72967684630139
8	28.281323844195903	34.17174418606647	19.7681894160651	17.778742553672526
9	29.501160902990513	15.07828609502297	18.46876457992402	36.95178842206249
10-11	27.02776772440711	25.073658458482782	26.569059448970034	21.329514368140075
12-13	26.986613737423788	22.046191733197087	27.085435731219277	23.88175879815985
14-15	24.856768951922486	23.555007826955134	24.531901406106225	27.056321815016155
16-17	24.81947310512263	26.308945953810742	25.141860172521252	23.72972076854538
18-19	24.797817149099153	26.299920886835928	26.340686809303655	22.561575154761268
20-21	25.267126191400653	25.227895890756773	26.29835574019474	23.206622177647834
22-23	25.30049024308365	24.593189499648087	25.967513483443085	24.13880677382518
24-25	24.799672446621845	24.66870941550436	25.80838796344253	24.723230174431265
26-27	24.303717627480633	25.17109201352239	26.120859120537233	24.404331238459744
28-29	24.32895769284639	26.26937489960359	25.436031137329945	23.965636270220074
30-31	25.764500320589512	25.212026731824196	25.46322590977079	23.5602470378155
32-33	25.290577543873848	25.22461201742025	25.318913398426634	24.165897040279265
34-35	25.128565022459977	25.69440717072924	25.778913947305014	23.39811385950577
36-37	24.930680285697875	25.77203326111726	25.83173797825287	23.465548474931992
38-39	25.179119473539767	25.705411663357157	25.359385651220357	23.75608321188272
40-41	25.24782587675551	25.281711100055098	25.34540126173382	24.125061761455573
42-43	24.64227984844789	25.44710571740993	25.123726546094243	24.786887888047936
44-45	24.08736293289846	25.460191877870187	25.897572275640684	24.55487291359067
46-47	24.175469584690184	24.950470994905906	25.67607627749685	25.19798314290706
48-49	24.053851403426936	25.415982799939425	25.866175719560918	24.66399007707272
50-51	24.466366944245628	25.136730854123186	26.02970080616474	24.36720139546645
52-53	24.461620544466946	25.15619837367017	25.76600134991804	24.616179731944847
54-55	23.393214791007477	24.846453441796655	26.567844632671996	25.192487134523876
56-57	24.067954144229294	24.894003249992736	25.73733170950835	25.300710896269624
58-59	24.260051298058933	25.25226059605924	25.94403701633375	24.543651089548078
60-61	25.10089213725017	25.049489120366303	25.971074229608433	23.8785445127751
62-63	24.704638097080526	24.86273643930648	26.02753851123904	24.405086952373956
64-65	24.049748097499045	24.531361576367853	26.254457483261856	25.164432842871243
66-67	24.262536651818	24.880265511244893	25.7612897632511	25.095908073686008
68-69	23.703831718629925	24.982829481831157	25.99500665550554	25.318332144033374
70-71	23.787672630995345	24.701684261080487	26.02193185540657	25.488711252517593
72-73	23.58217525104169	25.507794331719403	25.445334033967974	25.46469638327093
74-75	23.4473032526553	25.12649081677956	25.770704409588625	25.655501520976507
76-77	23.199673609422252	24.461319865514167	26.006024588868783	26.3329819361948
78-79	23.107246612481465	24.601091920411612	26.36681523899406	25.92484622811286
80-81	23.562633014137973	24.95338156878446	25.631527062518973	25.852458354558593
82-83	23.485943626398512	24.984473612168916	25.905814522962338	25.623768238470234
84-85	23.378635097304397	25.147266665369383	25.42553394854854	26.04856428877768
86-87	22.73915559476563	25.466435423742244	25.685545620358536	26.10886336113359
88-89	22.670862221647184	24.801842956424462	26.083633292344715	26.443661529583633
90-91	23.355279469263937	24.19775258574157	26.161552332686817	26.285415612307677
92-93	23.544278385316762	24.21589141850613	25.818080690160617	26.42174950601649
94-95	23.10112056024241	24.544972734188246	25.698923182984846	26.654983522584498
96-97	22.302075402760604	24.370853014866817	26.16251429270797	27.164557289664604
98-99	21.952361493026785	24.15951506832315	26.170033257374918	27.718090181275144
100-101	22.36700317567899	24.41062070198507	26.063862747724688	27.15851337461125
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	174.0
1	177.5
2	242.0
3	334.5
4	315.0
5	300.0
6	492.0
7	957.5
8	1389.0
9	1528.5
10	1363.0
11	1164.0
12	1190.0
13	1305.5
14	1480.5
15	1695.5
16	1920.5
17	2089.0
18	2256.0
19	2507.5
20	2763.0
21	2936.5
22	3127.5
23	3458.5
24	3989.5
25	4755.5
26	5982.0
27	7947.0
28	10807.0
29	15235.0
30	21490.5
31	28801.0
32	37745.0
33	47057.5
34	56225.5
35	66259.5
36	78809.5
37	92008.0
38	102487.5
39	112654.5
40	123270.5
41	132860.5
42	143268.0
43	158393.5
44	175878.0
45	193743.0
46	213911.0
47	234963.5
48	256246.5
49	275555.5
50	290734.5
51	299034.0
52	300571.0
53	298829.0
54	290922.0
55	274945.5
56	254149.5
57	230305.0
58	201554.5
59	168331.0
60	137471.0
61	111578.0
62	88838.0
63	67950.0
64	49400.0
65	35931.5
66	26546.5
67	19720.0
68	14524.5
69	10503.5
70	7624.0
71	5562.5
72	4012.0
73	3118.5
74	2370.5
75	1621.0
76	1107.5
77	681.0
78	371.5
79	197.0
80	100.0
81	59.5
82	36.5
83	22.0
84	12.5
85	8.0
86	5.0
87	2.5
88	1.5
89	0.5
90	0.0
91	0.5
92	2.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22327037449294518
2	0.0
3	6.856145385934138E-5
4	0.012272500240822107
5	0.014243642039278172
6	0.10690444693017805
7	0.1372428902629366
8	0.15417756936619392
9	0.10954406290376269
10-11	0.11910838571714082
12-13	0.0946148063258911
14-15	0.1179942620919265
16-17	0.07256372872838043
18-19	0.0781257766727195
20-21	0.05536337399141817
22-23	0.07927418102486347
24-25	0.07357501017280571
26-27	0.05078689694630712
28-29	0.01808308345540129
30-31	0.033552261482415184
32-33	0.04024557341543339
34-35	0.046227560264660926
36-37	0.036800360359001485
38-39	0.038574387977611944
40-41	0.021528296511833193
42-43	0.0319582076801855
44-45	0.017903109639020517
46-47	0.029841372792278335
48-49	2.8281599716978317E-4
50-51	0.01726034600908919
52-53	0.008475909733361079
54-55	0.016026239839621047
56-57	0.010806999164578685
58-59	0.007421777380273704
60-61	0.031529698593564616
62-63	0.013498036228557834
64-65	0.014972107486533673
66-67	0.059451350677781394
68-69	0.07579468724150189
70-71	0.0339464898421064
72-73	0.056186111437730266
74-75	0.046810332622465325
76-77	0.2007136561732219
78-79	0.07475769525187935
80-81	0.015777704569380935
82-83	0.00968430535763197
84-85	0.02537630810968873
86-87	0.16652720124260778
88-89	0.3770708558629128
90-91	0.27550563215203094
92-93	0.2772282386802469
94-95	0.002331089431217607
96-97	0.006007697394424788
98-99	0.004936424677872579
100-101	0.005339223219296209
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5834182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.040751356267194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.59496108321912	37.07233690268844
2	12.343679930300953	12.10686676566469
3	4.503292886423956	6.6253460018269195
4	2.181667501697373	4.27962453971158
5	1.2807186900164496	3.1403703417210465
6	0.7923072915553107	2.3313206929752894
7	0.5395946479795762	1.8523488875317247
8	0.39384624465466306	1.5451612605367127
9	0.29671500655256766	1.3096014174015895
>10	1.8490472794624822	17.20744365477833
>50	0.15389141837232817	5.127366163370247
>100	0.06704261932952008	5.569053652450448
>500	0.0021100519655848646	0.6802492751192593
>1k	0.0010550141906343286	0.9375567760701266
>5k	7.033427936580167E-5	0.2153536681536291
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7375	0.12641018055316067	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7140363464835345E-5
2	0.0	0.0	0.0	0.0	1.7140363464835345E-5
3	0.0	0.0	0.0	0.0	1.7140363464835345E-5
4	0.0	0.0	0.0	0.0	1.7140363464835345E-5
5	0.0	0.0	0.0	1.7140363464835345E-5	1.7140363464835345E-5
6	0.0	0.0	0.0	1.7140363464835345E-5	1.7140363464835345E-5
7	0.0	0.0	0.0	1.7140363464835345E-5	1.7140363464835345E-5
8	0.0	0.0	0.0	1.7140363464835345E-5	3.428072692967069E-5
9	0.0	0.0	0.0	3.428072692967069E-5	5.1421090394506036E-5
10-11	0.0	0.0	0.0	3.428072692967069E-5	5.1421090394506036E-5
12-13	0.0	0.0	0.0	3.428072692967069E-5	5.1421090394506036E-5
14-15	0.0	0.0	0.0	3.428072692967069E-5	7.713163559175905E-5
16-17	0.0	0.0	0.0	3.428072692967069E-5	1.0284218078901207E-4
18-19	0.0	0.0	0.0	5.999127212692371E-5	1.0284218078901207E-4
20-21	0.0	0.0	0.0	7.713163559175905E-5	1.0284218078901207E-4
22-23	0.0	0.0	0.0	2.0568436157802415E-4	1.1141236252142974E-4
24-25	0.0	0.0	0.0	8.655883549741848E-4	1.1998254425384741E-4
26-27	0.0	0.0	0.0	0.0017054661647511167	1.542632711835181E-4
28-29	0.0	0.0	0.0	0.005921995577100611	1.542632711835181E-4
30-31	0.0	0.0	0.0	0.022856674680357934	1.542632711835181E-4
32-33	0.0	0.0	0.0	0.05963132449416217	1.9711417984560648E-4
34-35	0.0	0.0	0.0	0.10757292110530663	2.571054519725302E-4
36-37	0.0	8.570181732417673E-6	0.0	0.18456743378934698	2.571054519725302E-4
38-39	0.0	1.7140363464835345E-5	0.0	0.317919461545766	2.742458154373655E-4
40-41	0.0	1.7140363464835345E-5	0.0	0.4774276839495237	2.9138617890220087E-4
42-43	0.0	1.7140363464835345E-5	0.0	0.633276438753539	2.9138617890220087E-4
44-45	0.0	1.7140363464835345E-5	0.0	0.8136187729488041	3.085265423670362E-4
46-47	0.0	2.5710545197253018E-5	0.0	1.0131497440429524	3.170967240994539E-4
48-49	0.0	3.428072692967069E-5	0.0	1.2316722378561382	3.428072692967069E-4
50-51	8.570181732417673E-6	3.428072692967069E-5	0.0	1.4740198368854451	3.599476327615422E-4
52-53	1.7140363464835345E-5	3.428072692967069E-5	0.0	1.729805480871183	4.027985414236306E-4
54-55	1.7140363464835345E-5	3.428072692967069E-5	0.0	2.019366211064379	4.2850908662088363E-4
56-57	1.7140363464835345E-5	3.428072692967069E-5	0.0	2.32659865598982	4.370792683533013E-4
58-59	1.7140363464835345E-5	3.428072692967069E-5	0.0	2.6707257332733194	4.542196318181366E-4
60-61	1.7140363464835345E-5	3.428072692967069E-5	0.0	3.048456493129628	4.71359995282972E-4
62-63	2.5710545197253018E-5	3.428072692967069E-5	0.0	3.457399854855402	4.97070540480225E-4
64-65	3.428072692967069E-5	3.428072692967069E-5	0.0	3.8713396325311757	4.97070540480225E-4
66-67	3.428072692967069E-5	3.428072692967069E-5	0.0	4.3366576496927935	4.97070540480225E-4
68-69	3.428072692967069E-5	3.428072692967069E-5	0.0	4.851399904905263	4.97070540480225E-4
70-71	3.428072692967069E-5	3.428072692967069E-5	0.0	5.421856911560181	5.142109039450604E-4
72-73	3.428072692967069E-5	3.428072692967069E-5	0.0	5.996461886173588	6.256232664664901E-4
74-75	3.428072692967069E-5	3.428072692967069E-5	0.0	6.5828422904873385	6.427636299313254E-4
76-77	3.428072692967069E-5	5.1421090394506036E-5	0.0	7.2259830084148895	6.513338116637431E-4
78-79	3.428072692967069E-5	5.1421090394506036E-5	0.0	7.936159687853412	6.599039933961608E-4
80-81	3.428072692967069E-5	5.999127212692371E-5	0.0	8.662328326404626	6.770443568609962E-4
82-83	3.428072692967069E-5	6.856145385934138E-5	0.0	9.423403315151978	7.370356289879198E-4
84-85	3.428072692967069E-5	6.856145385934138E-5	0.0	10.20656366222377	7.370356289879198E-4
86-87	3.428072692967069E-5	6.856145385934138E-5	0.0	11.01318059669719	7.370356289879198E-4
88-89	3.428072692967069E-5	6.856145385934138E-5	0.0	11.884939482518714	7.541759924527551E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7000	0.0	47.720684	1
GTATCAA	12705	0.0	44.4199	1
TCAACGC	17660	0.0	31.491543	4
TATCAAC	17770	0.0	31.429157	2
CAACGCA	18230	0.0	30.454527	5
ATCAACG	18380	0.0	30.335442	3
GTACATG	32680	0.0	29.747734	1
AACGCAG	19165	0.0	29.113932	6
TACATGG	33905	0.0	28.378443	2
ACATGGG	35320	0.0	26.798738	3
CATGGGG	28810	0.0	25.963608	4
ATGGGGG	20750	0.0	25.199575	5
ATATGTA	4065	0.0	24.699574	1
TGGGGGG	18350	0.0	24.687899	6
GTAAGTA	6700	0.0	23.532936	2
ACGCAGA	23360	0.0	23.418089	7
CGCAGAG	23845	0.0	22.72232	8
TATGTAA	4545	0.0	22.152096	2
TGTAAGT	6975	0.0	22.10387	1
GAGTACA	24885	0.0	20.517744	1
>>END_MODULE