FastQCFastQC Report
Mon 15 Aug 2016
SRR2921575_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921575_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7919581
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT105480.13318886441088235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG444700.029.21181
TACATGG467900.027.6153242
ACATGGG492750.025.8493123
GTATCAA310550.024.4345131
ATATGTA32400.024.2865431
TATGTAA34300.023.8459012
CATGGGG396850.023.6197414
TACCGTA8100.022.0949887
ATGGGGG259550.021.8448495
GTATATG18200.021.4912721
TGTAAGT53450.020.834461
AGTACAT310200.020.7827852
GAGTACA334200.020.516081
TCAACGC371300.020.4435584
TGGGGGG218500.020.4387876
TATCAAC367600.020.4251122
GTAAGTA55450.020.3026352
CAACGCA373400.020.2293725
TAAGTAC91150.019.941371
ATCAACG384900.019.7455843
Produced by FastQC (version 0.11.5)