##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921575_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7919581
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.687378536819057	31.0	28.0	31.0	16.0	34.0
2	27.633951467887	31.0	27.0	31.0	15.0	34.0
3	27.722668535115684	31.0	27.0	33.0	16.0	34.0
4	31.09574875236455	35.0	32.0	37.0	16.0	37.0
5	31.005031074244965	35.0	32.0	37.0	16.0	37.0
6	31.099894678771516	35.0	32.0	37.0	16.0	37.0
7	30.890988424766412	35.0	31.0	37.0	11.0	37.0
8	30.831477195573857	35.0	31.0	37.0	10.0	37.0
9	32.006474584955946	37.0	32.0	39.0	10.0	39.0
10-11	31.746972219868702	37.0	31.0	39.0	10.0	39.0
12-13	31.509210462018128	37.0	30.0	39.0	10.0	39.0
14-15	32.12536572326238	37.0	31.0	40.0	8.0	41.0
16-17	31.949962062386888	37.0	30.5	40.0	7.5	41.0
18-19	31.750203009477396	37.0	30.0	40.0	2.0	41.0
20-21	31.595027060143714	37.0	30.0	40.0	2.0	41.0
22-23	31.364956492016432	37.0	30.0	40.0	2.0	41.0
24-25	31.45011888886546	37.0	30.0	40.0	2.0	41.0
26-27	31.174400135562728	37.0	30.0	40.0	2.0	41.0
28-29	30.95406208990097	36.5	29.0	40.0	2.0	41.0
30-31	30.672506790447628	36.0	28.5	40.0	2.0	41.0
32-33	30.3981002277772	36.0	28.0	39.0	2.0	41.0
34-35	30.039519956927016	35.5	27.0	39.0	2.0	40.5
36-37	29.718581450205512	35.0	26.5	39.0	2.0	40.0
38-39	29.475123305134453	35.0	26.0	39.0	2.0	40.0
40-41	29.224779505380397	35.0	25.0	39.0	2.0	40.0
42-43	28.948744952542313	35.0	24.5	39.0	2.0	40.0
44-45	28.664378519520163	35.0	24.0	39.0	2.0	40.0
46-47	28.334522369302114	34.0	23.5	38.0	2.0	40.0
48-49	28.005760405758842	34.0	23.0	38.0	2.0	40.0
50-51	27.110740075769158	33.0	21.5	37.5	2.0	39.5
52-53	27.339152475364543	33.5	22.5	37.5	2.0	39.0
54-55	27.876441001108518	34.0	23.0	38.0	2.0	40.0
56-57	27.824474110435894	34.0	22.5	38.0	2.0	40.0
58-59	27.546013103471005	34.0	22.0	38.0	2.0	40.0
60-61	27.149394835408593	34.0	21.0	38.0	2.0	40.0
62-63	26.684250214752524	33.0	19.5	37.0	2.0	40.0
64-65	26.206537757489947	33.0	18.0	36.5	2.0	39.0
66-67	25.837161082133008	32.0	15.5	36.0	2.0	39.0
68-69	25.36682685611777	32.0	10.5	35.5	2.0	39.0
70-71	24.929531625473622	32.0	7.5	35.0	2.0	38.0
72-73	24.444359998338296	31.0	6.5	35.0	2.0	37.0
74-75	23.99662198038002	31.0	2.0	35.0	2.0	36.5
76-77	23.579182282497015	31.0	2.0	34.5	2.0	36.0
78-79	23.182951156128084	30.5	2.0	34.0	2.0	36.0
80-81	22.7374318161529	30.0	2.0	34.0	2.0	35.0
82-83	22.194846356139294	29.5	2.0	34.0	2.0	35.0
84-85	21.778044886465587	29.0	2.0	34.0	2.0	35.0
86-87	21.296336637001378	29.0	2.0	34.0	2.0	35.0
88-89	20.778698330126304	29.0	2.0	34.0	2.0	35.0
90-91	20.16801406539058	27.5	2.0	34.0	2.0	35.0
92-93	19.5941269493929	27.0	2.0	33.5	2.0	35.0
94-95	18.889592265045334	25.5	2.0	33.0	2.0	35.0
96-97	18.236349119985007	25.0	2.0	33.0	2.0	34.5
98	17.76574972842629	24.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	577049.0
3	97781.0
4	73152.0
5	58295.0
6	53281.0
7	56355.0
8	63549.0
9	71027.0
10	74768.0
11	76986.0
12	77775.0
13	75380.0
14	73517.0
15	76417.0
16	80396.0
17	85860.0
18	88864.0
19	90253.0
20	92989.0
21	99007.0
22	106757.0
23	118302.0
24	132609.0
25	150270.0
26	170821.0
27	194516.0
28	223034.0
29	253633.0
30	291774.0
31	336438.0
32	393832.0
33	469906.0
34	568780.0
35	672724.0
36	781284.0
37	713678.0
38	283630.0
39	14892.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.149756150990754	16.628102878133745	12.556283440996644	24.665857529878863
2	16.864663520415295	19.02208761529901	38.241293278500905	25.87195558578479
3	21.178197068700662	23.052564137741456	27.146768415264162	28.622470378293723
4	14.533564003953414	14.154975406218117	34.271264046929154	37.04019654289931
5	15.556860325057023	37.26487440392452	31.697440641521784	15.480824629496666
6	35.57708851750257	31.686463466181973	17.02934059767613	15.707107418639335
7	31.55964315587716	30.93087376688417	18.690371559787156	18.819111517451514
8	29.42817824438697	32.47575077044273	18.698404836573193	19.3976661485971
9	29.36522118083754	15.166545289415605	17.789190508656763	37.679043021090095
10-11	27.952888540491287	24.05388416745039	25.262897642154137	22.730329649904185
12-13	28.02559960035903	20.962638721431755	25.91667521140007	25.095086466809143
14-15	25.605683086966845	22.458882109107346	23.46871645806089	28.466718345864916
16-17	25.651432614444808	25.38836055457151	24.076188739586442	24.88401809139724
18-19	25.640916478112434	25.45862089156691	25.100497864027577	23.799964766293076
20-21	26.245556183646883	24.084983810937278	25.180082981783	24.48937702363284
22-23	26.17386825749413	23.43078415449901	24.982959482559775	25.412388105447086
24-25	25.863117003488522	23.85697646611727	24.702505527526117	25.57740100286809
26-27	25.314695256705335	24.109389439230636	25.270040961012004	25.305874343052032
28-29	25.1828896151562	25.175836621833604	24.543677335920105	25.097596427090092
30-31	26.367381898212617	24.353985751896637	24.412992180684647	24.8656401692061
32-33	25.92367321530486	24.293975536601074	24.36252791570836	25.419823332385704
34-35	26.090600908497684	24.544071509602485	24.770261914177457	24.59506566772237
36-37	25.864378705830376	24.437373562152235	25.073955782380402	24.62429194963699
38-39	26.08930696648931	24.590890724647213	24.535918086460505	24.78388422240297
40-41	25.98212823358304	24.36420970798001	24.533569199001835	25.120092859435104
42-43	25.51858154461362	24.396922337051528	24.229501060524786	25.854995057810065
44-45	25.13981227976795	24.246533732158813	24.884337401897064	25.72931658617617
46-47	25.472289010567152	23.97210536153208	24.750322502146687	25.80528312575408
48-49	25.274208509555418	24.421768414508545	24.75401953555524	25.5500035403808
50-51	25.19752638382472	24.25317451420385	25.236439477282644	25.312859624688787
52-53	25.491437513338095	24.364143551103975	24.831363392815955	25.313055542741974
54-55	24.386419734314575	23.88034652637341	25.682738556824347	26.05049518248767
56-57	25.107525321929845	23.951044543462856	24.97163084896387	25.96979928564343
58-59	25.50320332136897	24.159598788883144	25.098317585736197	25.238880304011683
60-61	26.03094344116097	23.972445174105154	25.29866684908184	24.697944535652034
62-63	25.448374582363464	23.975842794483242	25.356141434294493	25.219641188858805
64-65	24.93016397023404	23.867350059687507	25.52552499286056	25.676960977217895
66-67	25.290857735848242	24.07178273498336	24.935691259244916	25.701668269923484
68-69	24.749783654962172	24.042861240792867	25.248002699697253	25.959352404547708
70-71	24.985326603000154	23.77960997797328	25.293314383031863	25.941749035994704
72-73	24.82517232364854	24.346298372609848	25.092353040950254	25.736176262791354
74-75	24.52591112721296	24.374277893649605	25.434229203637805	25.66558177549963
76-77	24.557434180851317	23.55640386487192	25.31019474712944	26.575967207147322
78-79	24.478759599697604	23.724053679991446	25.729851785600893	26.067334934710058
80-81	25.014525264718124	23.939016298049694	25.163139391770923	25.883319045461263
82-83	24.93261556207934	23.960699428117042	25.41875089821648	25.687934111587136
84-85	24.7225500246229	24.227148293416846	25.064637090614617	25.985664591345643
86-87	24.27027622075251	24.47320032570661	25.315535398754157	25.94098805478672
88-89	24.080888812738856	23.914673632621966	25.487034665087272	26.51740288955191
90-91	24.793759169661893	23.29226274414843	25.731428417682352	26.182549668507328
92-93	25.190058337012182	23.42090700577141	25.435331043775356	25.95370361344105
94-95	24.934332819611246	23.58483894549863	25.39321961552704	26.087608619363085
96-97	24.201834616116084	23.380972676258857	25.895326902579164	26.52186580504589
98	24.345945406616636	23.22094656201675	25.374840285718737	27.05826774564788
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	165.0
1	179.0
2	263.0
3	392.5
4	434.0
5	479.5
6	743.5
7	1306.5
8	1932.0
9	2205.0
10	2152.5
11	2175.0
12	2409.5
13	2795.0
14	3245.0
15	3629.5
16	3898.5
17	4068.0
18	4074.0
19	4029.5
20	3906.0
21	3652.0
22	3549.5
23	3583.0
24	3676.5
25	4063.0
26	4676.0
27	5395.0
28	6430.0
29	8272.0
30	11196.5
31	15848.0
32	23567.0
33	33275.0
34	44792.0
35	60008.0
36	77164.0
37	93215.0
38	109184.5
39	123978.5
40	137804.5
41	155235.5
42	178554.0
43	207272.0
44	235157.0
45	268375.0
46	314966.5
47	360199.0
48	388969.5
49	405908.5
50	413422.0
51	417191.5
52	425905.0
53	435772.0
54	437400.0
55	425997.0
56	404929.5
57	375513.0
58	337559.5
59	293505.5
60	249150.5
61	204198.5
62	161853.5
63	123007.5
64	88356.5
65	62204.5
66	43966.5
67	31417.0
68	21693.5
69	14732.5
70	10049.5
71	6825.5
72	4880.5
73	3585.0
74	2591.5
75	1804.0
76	1143.0
77	692.0
78	400.0
79	232.0
80	142.0
81	85.0
82	66.0
83	56.0
84	37.5
85	33.0
86	31.5
87	29.5
88	30.0
89	26.0
90	19.0
91	16.0
92	14.0
93	14.0
94	13.5
95	9.0
96	8.5
97	11.0
98	8.5
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2812775069792202
2	0.14043672259933954
3	0.1381133673612278
4	0.13217870995952943
5	0.12801182284769863
6	0.046151431496186474
7	0.035948871537522
8	0.013662338954548226
9	0.009394436397582145
10-11	0.0018814126656448112
12-13	0.005972538193624133
14-15	0.010814966094797188
16-17	0.00578313423399546
18-19	0.04880940039630884
20-21	0.09600886713577397
22-23	0.09540908793028319
24-25	0.07296471871428552
26-27	0.08293999392139559
28-29	0.10160259741014077
30-31	0.07622246681989868
32-33	0.019546488633678978
34-35	0.29772408414030993
36-37	0.3860873447724065
38-39	0.3583838589440527
40-41	0.3546084066821212
42-43	0.3323281875644684
44-45	0.3165192704007952
46-47	0.3213238175100425
48-49	0.31504191951569155
50-51	0.3165066434701533
52-53	0.36174893596012214
54-55	0.3477582968088842
56-57	0.3323913222176779
58-59	0.31421485555864637
60-61	0.32199935829938475
62-63	0.32143114642049875
64-65	0.3384901297177212
66-67	0.3579987375594744
68-69	0.3202505284054801
70-71	0.2965876603825379
72-73	0.2816373745025147
74-75	0.2682780818833724
76-77	0.2717126070179723
78-79	0.2778114145180155
80-81	0.26862532247602494
82-83	0.2776156970930659
84-85	0.27079084108111273
86-87	0.26594209971461874
88-89	0.2890241289280329
90-91	0.27265962681611566
92-93	0.26291794982588096
94-95	0.2776725182809545
96-97	0.2766181695723549
98	0.2763277501675909
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	7919581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.85106096311692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.12340276078022	30.335983641397558
2	9.992233397362494	7.964022045519712
3	4.304482356757038	5.146145664413577
4	2.3853003572528295	3.8022699980890833
5	1.515930807386215	3.0205725510507557
6	0.9865238356722953	2.358841291016675
7	0.6984069338987905	1.9482580109904988
8	0.5131333718811814	1.6359127428037363
9	0.40443941597240013	1.4505605839623168
>10	2.624484008769865	20.257721935996067
>50	0.2866632518395564	7.924492214402116
>100	0.15768094542544395	11.12061652871855
>500	0.005545458129273752	1.5060627740588561
>1k	0.0016763843897253023	1.2435880319847663
>5k	6.447632165691712E-5	0.1494392018235901
>10k+	3.223816082845856E-5	0.13551278377194192
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	10548	0.13318886441088235	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2626930641911484E-5
2	0.0	0.0	0.0	1.2626930641911484E-5	1.2626930641911484E-5
3	0.0	0.0	0.0	1.2626930641911484E-5	1.2626930641911484E-5
4	0.0	0.0	0.0	1.2626930641911484E-5	3.788079192573446E-5
5	1.2626930641911484E-5	1.2626930641911484E-5	0.0	1.2626930641911484E-5	3.788079192573446E-5
6	1.2626930641911484E-5	1.2626930641911484E-5	0.0	1.2626930641911484E-5	3.788079192573446E-5
7	1.2626930641911484E-5	1.2626930641911484E-5	0.0	3.788079192573446E-5	5.050772256764594E-5
8	1.2626930641911484E-5	1.2626930641911484E-5	0.0	3.788079192573446E-5	5.050772256764594E-5
9	1.2626930641911484E-5	1.2626930641911484E-5	0.0	5.050772256764594E-5	6.313465320955743E-5
10-11	1.2626930641911484E-5	1.2626930641911484E-5	0.0	6.944811853051318E-5	6.313465320955743E-5
12-13	1.2626930641911484E-5	1.2626930641911484E-5	0.0	8.838851449338039E-5	1.0101544513529187E-4
14-15	1.2626930641911484E-5	1.2626930641911484E-5	0.0	9.470197981433613E-5	1.2626930641911486E-4
16-17	1.2626930641911484E-5	1.2626930641911484E-5	0.0	1.0101544513529187E-4	1.641500983448493E-4
18-19	1.2626930641911484E-5	1.2626930641911484E-5	0.0	1.3889623706102633E-4	1.7677702898676078E-4
20-21	1.2626930641911484E-5	1.2626930641911484E-5	0.0	1.7677702898676078E-4	1.7677702898676078E-4
22-23	1.2626930641911484E-5	1.2626930641911484E-5	0.0	3.0304633540587567E-4	1.9571742494962801E-4
24-25	1.2626930641911484E-5	1.8940395962867226E-5	0.0	5.61898413565061E-4	2.1465782091249525E-4
26-27	1.2626930641911484E-5	2.525386128382297E-5	0.0	0.0013195142520797502	2.2728475155440672E-4
28-29	1.2626930641911484E-5	3.1567326604778715E-5	0.0	0.004223708299719391	2.2728475155440672E-4
30-31	1.2626930641911484E-5	3.788079192573446E-5	0.0	0.015133376374330914	2.3359821687536246E-4
32-33	1.2626930641911484E-5	3.788079192573446E-5	0.0	0.03853739231911385	2.525386128382297E-4
34-35	2.525386128382297E-5	3.788079192573446E-5	0.0	0.06926502803620545	2.7779247412205267E-4
36-37	3.788079192573446E-5	3.788079192573446E-5	0.0	0.12134480346876939	2.841059394430084E-4
38-39	3.788079192573446E-5	3.788079192573446E-5	0.0	0.2106550838990093	2.9041940476396414E-4
40-41	3.788079192573446E-5	3.788079192573446E-5	0.0	0.3160457605017235	2.9041940476396414E-4
42-43	3.788079192573446E-5	3.788079192573446E-5	0.0	0.41688442860802866	2.9041940476396414E-4
44-45	3.788079192573446E-5	5.050772256764594E-5	0.0	0.5363920136684	2.967328700849199E-4
46-47	3.788079192573446E-5	5.050772256764594E-5	0.0	0.6762289570622486	3.0304633540587567E-4
48-49	3.788079192573446E-5	5.050772256764594E-5	0.0	0.8286802041673669	3.1567326604778714E-4
50-51	3.788079192573446E-5	5.050772256764594E-5	0.0	1.0044597056334168	3.1567326604778714E-4
52-53	3.788079192573446E-5	5.050772256764594E-5	0.0	1.1871903324178388	3.283001966896986E-4
54-55	3.788079192573446E-5	5.050772256764594E-5	0.0	1.389132834173929	3.283001966896986E-4
56-57	3.788079192573446E-5	6.313465320955743E-5	0.0	1.6193659740332222	3.3461366201065435E-4
58-59	4.4194257246690194E-5	6.313465320955743E-5	0.0	1.8764376549718982	3.472405926525658E-4
60-61	5.050772256764594E-5	6.313465320955743E-5	0.0	2.1605309674842648	3.5355405797352156E-4
62-63	5.050772256764594E-5	6.313465320955743E-5	0.0	2.4627881197250208	3.5355405797352156E-4
64-65	5.050772256764594E-5	6.313465320955743E-5	0.0	2.7734611212386113	3.6618098861543303E-4
66-67	5.682118788860168E-5	6.313465320955743E-5	0.0	3.1243130160547636	3.9143484989925603E-4
68-69	6.313465320955743E-5	6.944811853051318E-5	0.0	3.5191192564354097	3.9143484989925603E-4
70-71	6.313465320955743E-5	7.576158385146892E-5	0.0	3.959477149106752	3.9143484989925603E-4
72-73	7.576158385146892E-5	7.576158385146892E-5	0.0	4.402568772262068	4.230021765040347E-4
74-75	7.576158385146892E-5	8.838851449338039E-5	0.0	4.851677127868255	4.4194257246690197E-4
76-77	8.207504917242465E-5	8.838851449338039E-5	0.0	5.334751926901183	4.4194257246690197E-4
78-79	8.838851449338039E-5	8.838851449338039E-5	0.0	5.886933917337293	4.4194257246690197E-4
80-81	8.838851449338039E-5	1.0101544513529187E-4	0.0	6.479881953350815	4.4194257246690197E-4
82-83	1.0101544513529187E-4	1.0101544513529187E-4	0.0	7.087268884553362	4.671964337507249E-4
84-85	1.0101544513529187E-4	1.0101544513529187E-4	0.0	7.6967392593118245	4.671964337507249E-4
86	1.1364237577720336E-4	1.0101544513529187E-4	0.0	8.163310659995775	4.671964337507249E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	44470	0.0	29.2118	1
TACATGG	46790	0.0	27.615324	2
ACATGGG	49275	0.0	25.849312	3
GTATCAA	31055	0.0	24.434513	1
ATATGTA	3240	0.0	24.286543	1
TATGTAA	3430	0.0	23.845901	2
CATGGGG	39685	0.0	23.619741	4
TACCGTA	810	0.0	22.094988	7
ATGGGGG	25955	0.0	21.844849	5
GTATATG	1820	0.0	21.491272	1
TGTAAGT	5345	0.0	20.83446	1
AGTACAT	31020	0.0	20.782785	2
GAGTACA	33420	0.0	20.51608	1
TCAACGC	37130	0.0	20.443558	4
TGGGGGG	21850	0.0	20.438787	6
TATCAAC	36760	0.0	20.425112	2
GTAAGTA	5545	0.0	20.302635	2
CAACGCA	37340	0.0	20.229372	5
TAAGTAC	9115	0.0	19.94137	1
ATCAACG	38490	0.0	19.745584	3
>>END_MODULE