FastQCFastQC Report
Mon 15 Aug 2016
SRR2921575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7919581
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT111410.14067663428153587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG459050.027.2012081
TACATGG485550.025.523312
GTATCAA324450.024.4056241
ACATGGG500850.024.2238883
ATATGTA35850.024.1583141
GTAAGTA65600.023.813462
TGTAAGT65750.022.6533761
CATGGGG398100.022.4843224
TTATATG30350.022.3721522
TACCGTA7500.021.524027
TATGTAA42050.021.3415642
ATGGGGG255700.020.9105555
TGGGGGG201200.020.907956
TAAGTAC101800.020.848341
TATCAAC389500.020.053442
TCAACGC391300.019.9747184
TATATGT32100.019.970523
ATTATAT39850.019.9421751
GTATAGG16750.019.8869231
CAACGCA395550.019.6999475
Produced by FastQC (version 0.11.5)