##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921575_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7919581
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.111280382131326	31.0	30.0	34.0	26.0	34.0
2	30.07700066455536	31.0	30.0	34.0	26.0	34.0
3	30.256650951609686	31.0	30.0	34.0	26.0	34.0
4	33.92404181483844	35.0	35.0	37.0	30.0	37.0
5	33.452888984909684	35.0	35.0	37.0	28.0	37.0
6	33.357575987921585	35.0	33.0	37.0	28.0	37.0
7	33.17117584882332	35.0	33.0	37.0	27.0	37.0
8	33.06861057422103	35.0	33.0	37.0	26.0	37.0
9	34.372994480389806	37.0	34.0	39.0	27.0	39.0
10-11	34.15784982816642	37.0	33.5	39.0	26.0	39.0
12-13	33.95873525379689	37.0	33.0	39.0	25.0	39.0
14-15	34.792892831072756	38.0	33.0	40.0	25.0	41.0
16-17	34.64017300915288	38.0	33.0	40.0	25.0	41.0
18-19	34.505876510386095	38.0	33.0	40.0	24.0	41.0
20-21	34.35723487139029	38.0	33.0	40.0	23.5	41.0
22-23	34.194014872756526	38.0	33.0	40.0	22.5	41.0
24-25	34.01649942086583	38.0	33.0	40.0	22.0	41.0
26-27	33.917779425451926	38.0	32.5	40.0	21.5	41.0
28-29	33.93259813871467	38.0	33.0	40.0	21.5	41.0
30-31	33.766802119960644	38.0	32.5	40.0	19.5	41.0
32-33	33.58479356420497	38.0	32.0	40.0	18.5	41.0
34-35	33.37187314581415	38.0	32.0	40.0	18.0	41.0
36-37	33.199744784477865	38.0	32.0	40.0	16.5	41.0
38-39	33.01931440564848	37.0	31.5	40.0	16.0	41.0
40-41	33.03550787598485	38.0	32.0	40.0	15.5	41.0
42-43	32.82902592952834	37.0	31.0	40.0	15.0	41.0
44-45	32.617283098689185	37.0	31.0	40.0	12.5	41.0
46-47	32.33176048076281	37.0	31.0	40.0	9.0	41.0
48-49	32.02756212733982	37.0	30.5	40.0	8.0	41.0
50-51	31.742656271840644	36.0	30.0	39.5	7.0	41.0
52-53	31.405695200289006	36.0	30.0	39.0	4.0	41.0
54-55	31.07063025177721	35.5	29.0	39.0	2.0	40.0
56-57	30.705062553183055	35.0	29.0	39.0	2.0	40.0
58-59	30.1996116966289	35.0	28.0	38.5	2.0	40.0
60-61	29.799587440295134	34.0	28.0	38.0	2.0	40.0
62-63	29.42378555128106	34.0	27.5	38.0	2.0	40.0
64-65	28.967618552042083	34.0	26.5	37.0	2.0	39.5
66-67	28.53101249927237	34.0	26.0	36.5	2.0	39.0
68-69	28.084916866182695	33.0	26.0	36.0	2.0	39.0
70-71	27.619454930759595	33.0	25.5	35.5	2.0	38.5
72-73	27.140219981334873	33.0	25.0	35.0	2.0	37.5
74-75	26.610417015748688	32.0	23.5	35.0	2.0	37.0
76-77	25.162040327638547	30.5	21.0	34.0	2.0	35.0
78-79	26.05656877554507	32.0	23.5	34.5	2.0	36.0
80-81	26.12382530085872	32.0	23.5	35.0	2.0	36.0
82-83	25.81881516206476	32.0	22.5	35.0	2.0	35.0
84-85	25.411869706238246	32.0	20.0	34.5	2.0	35.0
86-87	25.00006925871457	31.5	19.5	34.0	2.0	35.0
88-89	24.57386274602154	31.0	17.5	34.0	2.0	35.0
90-91	24.153284182584912	31.0	12.5	34.0	2.0	35.0
92-93	23.732891298163374	31.0	7.0	34.0	2.0	35.0
94-95	23.337343415011475	31.0	2.0	34.0	2.0	35.0
96-97	22.796549842725266	31.0	2.0	34.0	2.0	35.0
98-99	22.04233708828788	30.0	2.0	34.0	2.0	35.0
100-101	19.439450900243333	26.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	195333.0
3	92354.0
4	51457.0
5	36513.0
6	31830.0
7	31549.0
8	35757.0
9	40732.0
10	46562.0
11	51400.0
12	57031.0
13	61849.0
14	66697.0
15	71748.0
16	75155.0
17	77463.0
18	77041.0
19	75717.0
20	76488.0
21	79386.0
22	86117.0
23	95543.0
24	111142.0
25	130928.0
26	154572.0
27	183009.0
28	214845.0
29	251970.0
30	295498.0
31	348515.0
32	416715.0
33	508403.0
34	639138.0
35	786332.0
36	988662.0
37	987326.0
38	375360.0
39	13444.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.17517776292219	17.959541612002347	13.260325512886942	24.60495511218852
2	16.787681065450304	20.03441848754372	37.23296724915119	25.944933197854787
3	20.92120048300547	23.792868282518555	27.533307010385272	27.7526242240907
4	15.10183809029855	15.075280362094357	33.86028260807246	35.96259893953464
5	15.539756105353858	37.47972922372509	31.818990634367793	15.161524036553256
6	35.527379727636756	32.31506484481069	16.759780632017122	15.397774795535435
7	31.417306049083226	31.06000125933533	18.776285147909945	18.7464075436715
8	29.344756114062566	33.0042655221123	18.567806373370395	19.08317199045474
9	29.0324260629085	15.17312262877099	17.852334893443064	37.94211641487745
10-11	27.869752517419364	24.329939185739587	25.336034644236484	22.464273652604565
12-13	27.96460074091913	21.094001737443552	26.11256831934028	24.828829202297033
14-15	25.51794896300225	22.628984182071623	23.471208862252087	28.381857992674036
16-17	25.612395852254327	25.624867520118777	24.102306847887036	24.660429779739857
18-19	25.637991577625986	25.571611882357185	25.114441159859126	23.675955380157703
20-21	26.155031183460526	24.156165426091423	25.222085667281586	24.46671772316647
22-23	26.11532012327078	23.553775404505142	25.025517131830156	25.305387340393924
24-25	25.88947035562135	23.967812309867647	24.710708191099325	25.432009143411683
26-27	25.310658149828935	24.248839066685164	25.265260534432326	25.175242249053582
28-29	25.23005402783987	25.29026387510198	24.618313139196832	24.861368957861316
30-31	26.278171884064673	24.485728241095284	24.445858688360552	24.79024118647949
32-33	25.934148748588242	24.409258894575014	24.365470820013645	25.2911215368231
34-35	26.034674369976795	24.693176639566754	24.771283504993477	24.50086548546297
36-37	25.78282914407194	24.6273812095237	25.075941020656412	24.513848625747947
38-39	26.075244113921244	24.650865444428085	24.56354825236456	24.710342189286113
40-41	25.959564091806147	24.473251076622915	24.57905679522151	24.988128036349426
42-43	25.491928235913104	24.545152726008038	24.27650758282462	25.686411455254238
44-45	25.105724438782122	24.339978424460227	24.899074615955644	25.655222520802006
46-47	25.379943327189956	24.10798488682963	24.826168037553174	25.685903748427236
48-49	25.15850334406669	24.535443874392342	24.815504014180608	25.490548767360366
50-51	25.111802174906074	24.364197944817697	25.248922517629264	25.275077362646964
52-53	25.468506506605987	24.385744332224363	24.802430282891713	25.343318878277938
54-55	24.436753816730267	23.982520986838583	25.53837972210183	26.042345474329327
56-57	25.138574856878964	24.064135500438862	24.84218911494002	25.955100527742154
58-59	25.49516206205022	24.258903269401223	24.96167116619794	25.28426350235062
60-61	25.992113087926526	24.184162087271872	25.098434831550225	24.725289993251373
62-63	25.457252909911794	24.17139702159704	25.083888580961716	25.287461487529455
64-65	24.899752500567665	24.035973419889515	25.28928185257581	25.774992226967008
66-67	25.25943121940206	24.263405354517097	24.674760650762334	25.802402775318505
68-69	24.79164705384328	24.242801804232325	24.95095477856833	26.014596363356063
70-71	24.983582794440164	23.98041227490366	24.964080455233102	26.071924475423074
72-73	24.885936329010743	24.534838533294185	24.687731986947835	25.891493150747237
74-75	24.512928545504366	24.590398882860683	24.94009865924838	25.956573912386578
76-77	24.558331383717643	23.772618336189325	25.017445317234	26.651604962859032
78-79	24.45249552757776	23.95220469534518	25.355560253576897	26.239739523500166
80-81	24.905513476650682	24.23247972266862	24.792301402127347	26.069705398553356
82-83	24.76971906493088	24.225996989550687	25.056595095830446	25.94768884968798
84-85	24.566444658326773	24.51302599996426	24.64597587755097	26.274553464158
86-87	24.136871413341083	24.736823931671932	24.85204982564969	26.274254829337295
88-89	23.964349501730943	24.308599364226822	24.95575437717493	26.7712967568673
90-91	24.531503144816142	23.71697648666784	25.15090046472842	26.6006199037876
92-93	24.85967998102751	23.889511323509144	24.85267798723193	26.398130708231417
94-95	24.51957385921146	24.10336185127121	24.794563073989874	26.58250121552746
96-97	23.790651640404423	23.900847681769644	25.301719514106995	27.00678116371894
98-99	23.6077459443791	23.85280339268156	25.098250591744947	27.441200071194388
100-101	24.091638852240745	23.772374870440675	25.113073975597388	27.022912301721195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	151.0
1	149.5
2	211.5
3	289.0
4	265.0
5	281.5
6	504.0
7	975.0
8	1446.5
9	1545.5
10	1300.0
11	1071.0
12	1082.5
13	1184.0
14	1292.0
15	1424.0
16	1638.5
17	1845.5
18	2015.0
19	2200.5
20	2319.0
21	2438.0
22	2600.5
23	2847.5
24	3080.0
25	3396.5
26	3902.5
27	4640.0
28	5500.0
29	6841.5
30	9351.0
31	12937.5
32	19143.0
33	27310.5
34	36515.5
35	48595.0
36	64915.5
37	81164.5
38	95281.5
39	110843.5
40	125143.0
41	138171.5
42	154622.0
43	178701.5
44	206576.0
45	235367.5
46	269339.5
47	311313.0
48	361236.0
49	390810.5
50	403198.5
51	416061.5
52	425996.0
53	434709.5
54	435212.0
55	425442.0
56	405566.5
57	374459.5
58	337033.0
59	295646.0
60	251189.5
61	206444.0
62	162667.0
63	122981.0
64	87967.0
65	61260.5
66	42871.5
67	30079.0
68	20771.5
69	13970.5
70	9365.0
71	6435.5
72	4565.5
73	3437.5
74	2511.5
75	1631.5
76	1030.5
77	612.0
78	340.5
79	193.5
80	101.0
81	57.5
82	31.0
83	10.0
84	6.0
85	8.0
86	5.0
87	1.0
88	2.0
89	2.5
90	1.0
91	3.0
92	3.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.23141375787431182
2	0.0
3	2.525386128382297E-5
4	0.012399645890357079
5	0.014672493405901146
6	0.10490453977300063
7	0.13443893054443157
8	0.15140952532716062
9	0.10857897658979684
10-11	0.11803023417526762
12-13	0.09320568853326963
14-15	0.11643924091438676
16-17	0.07126008307762746
18-19	0.07596992820706044
20-21	0.052616419984845154
22-23	0.07917085512478501
24-25	0.0717967276299087
26-27	0.05009103385646286
28-29	0.01819540705499445
30-31	0.03171884977248165
32-33	0.03841112301269474
34-35	0.04423845150393689
36-37	0.036756995098604334
38-39	0.03840480954737378
40-41	0.022311786444257596
42-43	0.03087915888479454
44-45	0.01710949101979006
46-47	0.03005840839307029
48-49	4.040617805411675E-4
50-51	0.01709686408914815
52-53	0.008428476203475917
54-55	0.016225605874856257
56-57	0.010688696788378072
58-59	0.007677173830282183
60-61	0.030481410569574326
62-63	0.013157261728871767
64-65	0.01488715122681364
66-67	0.05714317461997043
68-69	0.07533226820964392
70-71	0.03260273491741546
72-73	0.053853859187752484
74-75	0.0460630429816931
76-77	0.20599574649214397
78-79	0.07682224602538948
80-81	0.015859424886240824
82-83	0.010474038967465578
84-85	0.024439424257419677
86-87	0.16884731654364038
88-89	0.3765035044151957
90-91	0.27454103948176045
92-93	0.27565220937824864
94-95	0.0022665340502231114
96-97	0.006471301953979636
98-99	0.005271743542998045
100-101	0.0054169532453800275
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	7919581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.982751249863114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.08854084334718	24.157842001952012
2	11.733348692535381	7.974629398926729
3	5.111768242725686	5.211358459184907
4	2.989587089306517	4.063775775828226
5	1.8544767981188197	3.1510111864557238
6	1.253247457714731	2.5553277966025827
7	0.8787850815864344	2.0904474380749725
8	0.6636894764315748	1.8043195507780874
9	0.5123619378262978	1.5670321454744018
>10	3.31590006681522	21.738772436131157
>50	0.3704665473187056	8.777635669157288
>100	0.21732990105296418	13.273885266925332
>500	0.007761080776873414	1.7582741025196895
>1k	0.002586823654657287	1.5185045110217696
>5k	1.1247059189567361E-4	0.21524568729632051
>10k+	3.749019729855788E-5	0.14193857367080837
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	11141	0.14067663428153587	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.788079192573446E-5
7	0.0	0.0	0.0	0.0	3.788079192573446E-5
8	0.0	0.0	0.0	0.0	3.788079192573446E-5
9	0.0	0.0	0.0	0.0	3.788079192573446E-5
10-11	0.0	0.0	0.0	6.313465320955742E-6	5.050772256764594E-5
12-13	0.0	0.0	0.0	3.788079192573445E-5	5.682118788860168E-5
14-15	0.0	0.0	0.0	5.682118788860168E-5	7.576158385146892E-5
16-17	0.0	0.0	0.0	7.576158385146892E-5	1.1364237577720336E-4
18-19	0.0	0.0	0.0	1.0101544513529187E-4	1.5152316770293783E-4
20-21	0.0	0.0	0.0	1.5152316770293783E-4	1.641500983448493E-4
22-23	0.0	0.0	0.0	2.967328700849199E-4	1.7046356366580504E-4
24-25	0.0	0.0	0.0	5.871522748488841E-4	1.8940395962867228E-4
26-27	0.0	0.0	0.0	0.0013700219746473961	1.8940395962867228E-4
28-29	0.0	0.0	0.0	0.004255275626324171	1.8940395962867228E-4
30-31	0.0	1.2626930641911484E-5	0.0	0.015108122513047093	1.8940395962867228E-4
32-33	0.0	1.2626930641911484E-5	0.0	0.03874573667470539	2.5885207815918546E-4
34-35	0.0	1.2626930641911484E-5	0.0	0.07043933258590322	2.651655434801412E-4
36-37	0.0	1.2626930641911484E-5	0.0	0.12366815870688108	2.651655434801412E-4
38-39	0.0	1.2626930641911484E-5	0.0	0.21563009457192245	2.7779247412205267E-4
40-41	0.0	1.2626930641911484E-5	0.0	0.3240386075980535	2.7779247412205267E-4
42-43	0.0	1.2626930641911484E-5	0.0	0.4276615139109001	2.841059394430084E-4
44-45	0.0	1.2626930641911484E-5	0.0	0.5508182819267837	2.9041940476396414E-4
46-47	0.0	1.2626930641911484E-5	0.0	0.6937046290706541	2.9041940476396414E-4
48-49	0.0	1.2626930641911484E-5	0.0	0.8476142866649132	3.0304633540587567E-4
50-51	0.0	1.2626930641911484E-5	0.0	1.0237599691195785	3.0304633540587567E-4
52-53	0.0	1.2626930641911484E-5	6.313465320955742E-6	1.2074376157021438	3.0304633540587567E-4
54-55	0.0	1.2626930641911484E-5	1.2626930641911484E-5	1.409114951914754	3.0304633540587567E-4
56-57	0.0	1.2626930641911484E-5	1.2626930641911484E-5	1.6381674737590284	3.093598007268314E-4
58-59	0.0	1.2626930641911484E-5	1.2626930641911484E-5	1.8939448943069084	3.1567326604778714E-4
60-61	0.0	1.2626930641911484E-5	1.2626930641911484E-5	2.177855116324967	3.219867313687429E-4
62-63	0.0	1.2626930641911484E-5	1.2626930641911484E-5	2.480712047771214	3.409271273316101E-4
64-65	0.0	1.2626930641911484E-5	1.2626930641911484E-5	2.7915113185912235	3.472405926525658E-4
66-67	0.0	1.2626930641911484E-5	1.2626930641911484E-5	3.14152983598501	3.5355405797352156E-4
68-69	0.0	1.2626930641911484E-5	1.2626930641911484E-5	3.5358625664665846	3.5355405797352156E-4
70-71	0.0	1.2626930641911484E-5	1.2626930641911484E-5	3.9757911434961017	3.661809886154331E-4
72-73	0.0	1.2626930641911484E-5	1.2626930641911484E-5	4.420562148426791	4.482560377878577E-4
74-75	0.0	1.2626930641911484E-5	1.2626930641911484E-5	4.873995227777833	4.5456950310881344E-4
76-77	0.0	1.2626930641911484E-5	1.2626930641911484E-5	5.363579209556667	4.5456950310881344E-4
78-79	0.0	1.2626930641911484E-5	1.2626930641911484E-5	5.9236214643173675	4.5456950310881344E-4
80-81	0.0	1.2626930641911484E-5	1.2626930641911484E-5	6.523179698521929	4.608829684297692E-4
82-83	0.0	1.2626930641911484E-5	1.2626930641911484E-5	7.141261639978175	4.735098990716807E-4
84-85	0.0	1.2626930641911484E-5	1.2626930641911484E-5	7.764716340422555	4.7982336439263644E-4
86-87	1.2626930641911484E-5	1.2626930641911484E-5	1.2626930641911484E-5	8.404314571692618	4.861368297135922E-4
88-89	1.2626930641911484E-5	1.2626930641911484E-5	1.2626930641911484E-5	9.101611562530897	4.987637603555037E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	45905	0.0	27.201208	1
TACATGG	48555	0.0	25.52331	2
GTATCAA	32445	0.0	24.405624	1
ACATGGG	50085	0.0	24.223888	3
ATATGTA	3585	0.0	24.158314	1
GTAAGTA	6560	0.0	23.81346	2
TGTAAGT	6575	0.0	22.653376	1
CATGGGG	39810	0.0	22.484322	4
TTATATG	3035	0.0	22.372152	2
TACCGTA	750	0.0	21.52402	7
TATGTAA	4205	0.0	21.341564	2
ATGGGGG	25570	0.0	20.910555	5
TGGGGGG	20120	0.0	20.90795	6
TAAGTAC	10180	0.0	20.84834	1
TATCAAC	38950	0.0	20.05344	2
TCAACGC	39130	0.0	19.974718	4
TATATGT	3210	0.0	19.97052	3
ATTATAT	3985	0.0	19.942175	1
GTATAGG	1675	0.0	19.886923	1
CAACGCA	39555	0.0	19.699947	5
>>END_MODULE