FastQCFastQC Report
Mon 15 Aug 2016
SRR2921574_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921574_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6986714
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108000.034.6413081
GGTATCA71950.030.4441341
TCAACGC132350.027.3939254
TATCAAC133600.027.2075822
CAACGCA134250.027.005265
GTACATG235750.026.3712751
ATCAACG140250.026.0152633
AACGCAG143850.025.3679586
TACATGG243800.024.8428612
ACATGGG264500.022.4290623
ACGCAGA173950.020.5248857
GAGTACA180150.020.179961
AGTACAT168850.020.003512
CGCAGAG181400.019.224518
CATGGGG217550.019.09464
GTATATA12350.019.0033741
TACACTG71000.016.697335
ACCGCTC31500.016.6063168
ATTCACG19750.016.493799
ATGGGGG115900.015.8981515
Produced by FastQC (version 0.11.5)