##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921574_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6986714
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.805432281899616	31.0	28.0	31.0	16.0	34.0
2	27.775836537748646	31.0	28.0	33.0	16.0	34.0
3	27.867035347375033	31.0	28.0	33.0	16.0	34.0
4	31.24432458520558	35.0	32.0	37.0	16.0	37.0
5	31.162083777867537	35.0	32.0	37.0	16.0	37.0
6	31.24924950413027	35.0	32.0	37.0	17.0	37.0
7	31.03923332198799	35.0	32.0	37.0	15.0	37.0
8	30.98042069562315	35.0	32.0	37.0	11.0	37.0
9	32.18679997492384	37.0	32.0	39.0	11.0	39.0
10-11	31.950206348792868	37.0	31.5	39.0	10.0	39.0
12-13	31.739196137125408	37.0	31.0	39.0	10.0	39.0
14-15	32.41518895721222	37.5	31.0	40.0	10.0	41.0
16-17	32.2535934632504	37.0	31.0	40.0	9.0	41.0
18-19	32.05692146551297	37.0	31.0	40.0	8.0	41.0
20-21	31.915059568775824	37.0	30.0	40.0	4.5	41.0
22-23	31.678785406129407	37.0	30.0	40.0	2.0	41.0
24-25	31.783913009749647	37.0	30.0	40.0	2.0	41.0
26-27	31.528723660364513	37.0	30.0	40.0	2.0	41.0
28-29	31.32150550315928	37.0	30.0	40.0	2.0	41.0
30-31	31.065681449104687	37.0	29.5	40.0	2.0	41.0
32-33	30.820252410503706	36.0	29.0	40.0	2.0	41.0
34-35	30.467403989915717	36.0	28.5	39.5	2.0	41.0
36-37	30.167003544155378	36.0	27.0	39.0	2.0	40.5
38-39	29.93751798055567	35.5	27.0	39.0	2.0	40.0
40-41	29.693502696689748	35.0	26.5	39.0	2.0	40.0
42-43	29.433813449355448	35.0	26.0	39.0	2.0	40.0
44-45	29.1652736178982	35.0	25.0	39.0	2.0	40.0
46-47	28.86320801166328	35.0	24.0	39.0	2.0	40.0
48-49	28.560637447017296	34.5	24.0	39.0	2.0	40.0
50-51	27.662343198819933	33.5	23.5	37.5	2.0	39.5
52-53	27.873816289030866	33.5	23.5	38.0	2.0	39.5
54-55	28.42660991991371	34.0	24.0	38.5	2.0	40.0
56-57	28.389559598403483	34.0	24.0	39.0	2.0	40.0
58-59	28.124870432652603	34.0	23.0	38.5	2.0	40.0
60-61	27.761201188427066	34.0	23.0	38.0	2.0	40.0
62-63	27.325255978704725	34.0	22.0	38.0	2.0	40.0
64-65	26.841598353675277	33.0	20.5	37.0	2.0	40.0
66-67	26.475361593447218	33.0	19.5	36.5	2.0	39.0
68-69	26.009215276308723	32.5	18.0	36.0	2.0	39.0
70-71	25.568603566712476	32.0	14.5	35.5	2.0	39.0
72-73	25.078582506740652	32.0	10.0	35.0	2.0	38.0
74-75	24.620876995966917	31.0	7.5	35.0	2.0	37.0
76-77	24.178843945809145	31.0	4.5	35.0	2.0	36.5
78-79	23.77507251620719	31.0	2.0	34.5	2.0	36.0
80-81	23.325540948148156	31.0	2.0	34.0	2.0	35.5
82-83	22.778657821115907	30.0	2.0	34.0	2.0	35.0
84-85	22.352543556241173	30.0	2.0	34.0	2.0	35.0
86-87	21.84633112218419	29.5	2.0	34.0	2.0	35.0
88-89	21.32799374641641	29.0	2.0	34.0	2.0	35.0
90-91	20.723899675870516	29.0	2.0	34.0	2.0	35.0
92-93	20.164708116004178	28.0	2.0	34.0	2.0	35.0
94-95	19.480918282900944	27.0	2.0	33.5	2.0	35.0
96-97	18.830357518570246	25.5	2.0	33.0	2.0	35.0
98	18.345121182862215	25.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	491220.0
3	81408.0
4	58644.0
5	47136.0
6	43511.0
7	45408.0
8	51013.0
9	58602.0
10	61668.0
11	64375.0
12	64416.0
13	62843.0
14	61079.0
15	63230.0
16	67818.0
17	71591.0
18	74528.0
19	76358.0
20	79268.0
21	84021.0
22	90747.0
23	99825.0
24	112171.0
25	127435.0
26	145318.0
27	166721.0
28	189594.0
29	216694.0
30	251248.0
31	290851.0
32	339930.0
33	405084.0
34	493632.0
35	602054.0
36	721291.0
37	695765.0
38	311045.0
39	19172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.36589356332446	16.66709961027899	12.651129014086932	25.315877812309616
2	16.522862912247106	18.407052554048413	38.608531166509515	26.461553367194973
3	20.668880589346198	21.680681759387756	27.60064373927155	30.04979391199449
4	14.055977468909687	14.295840369999668	34.33074854078744	37.317433620303206
5	15.623595589348033	35.92559833626062	32.97239501887069	15.478411055520652
6	36.11622906216274	32.501855421331356	16.31545040809246	15.066465108413455
7	31.628834574548964	30.20602547262553	18.885735140705943	19.27940481211956
8	28.193774709187057	33.90795449372595	18.523147510608005	19.375123286478985
9	27.52625614454117	15.742407149990717	18.09458495960323	38.636751745864885
10-11	27.087169106114985	24.341371855912755	26.132597084427424	22.43886195354484
12-13	27.2445768141966	21.297692741782246	26.502197686980526	24.955532757040633
14-15	24.421411349707018	22.91606203361544	23.513565335403953	29.14896128127359
16-17	24.964841960227652	26.123071246041007	24.24106227163377	24.67102452209757
18-19	24.98572498973452	25.6360497081812	25.511116966301667	23.867108335782607
20-21	25.543034964446885	24.62407007373223	25.36906406780732	24.463830894013565
22-23	25.37138204829358	24.353638328857965	25.12111144530825	25.1538681775402
24-25	24.979629123285303	24.72335497203364	25.181468739853052	25.115547164828
26-27	24.990862766160657	24.75101351136	25.301023778437965	24.95709994404138
28-29	25.085467624167674	25.05699990658806	25.025566503281738	24.831965965962524
30-31	25.376353788997807	24.845799203258583	24.856434358354594	24.921412649389016
32-33	25.020322798404067	24.841041251648175	24.998849382814665	25.139786567133093
34-35	25.213158063776426	24.822687960164227	25.19361482157192	24.770539154487427
36-37	25.201350106319293	24.89088283155694	25.06835222121194	24.83941484091183
38-39	25.366561944257647	24.816747753045085	24.989328597892662	24.827361704804602
40-41	25.302195026443165	24.735688638570018	25.003405616175243	24.958710718811574
42-43	25.133115944427338	24.62367665717998	25.050652802099155	25.192554596293526
44-45	25.058404845845306	24.721747434224607	25.110433522777154	25.109414197152937
46-47	25.2836068163984	24.649319068696716	24.987112451995188	25.0799616629097
48-49	24.99407076889063	24.71767241069903	25.087445391494352	25.200811428915987
50-51	25.017133026437943	24.74113104319188	25.105491850120636	25.136244080249536
52-53	24.97554339903018	24.846707274702315	25.166600046277594	25.011149279989915
54-55	24.68743876320139	24.503838768629898	25.476418254129136	25.33230421403958
56-57	24.850373969051645	24.64547840160637	25.262333311005108	25.241814318336882
58-59	24.945535332943315	24.71650019201711	25.321127983892094	25.01683649114747
60-61	25.19685220252069	24.38535867280345	25.35955866169074	25.058230462985126
62-63	24.884239833557476	24.49103358527229	25.504063360950756	25.120663220219473
64-65	24.78462118817736	24.37729155229697	25.552388584948105	25.285698674577567
66-67	24.783746957477284	24.695424571504653	25.306823164637265	25.2140053063808
68-69	24.691981147482437	24.50296064639254	25.3177638424621	25.487294363662926
70-71	24.7536781413519	24.31223515071272	25.464868722600002	25.46921798533538
72-73	24.7245787782015	24.50190962301746	25.40627792110106	25.367233677679973
74-75	24.531599533511663	24.452990480383395	25.58103326276504	25.434376723339902
76-77	24.55452823525174	24.16645487722503	25.63199237012691	25.64702451739632
78-79	24.534521833824243	24.141080161354857	25.69759655430342	25.626801450517476
80-81	24.663811492494585	24.31019848219917	25.49431847126446	25.53167155404179
82-83	24.524983479823355	24.356269224954183	25.585855442337103	25.53289185288536
84-85	24.646480533334213	24.344807144358292	25.512783413445444	25.495928908862048
86-87	24.440477878270073	24.38028103531108	25.633803160484923	25.545437925933918
88-89	24.441095739409953	24.103887298261974	25.692609259037052	25.762407703291018
90-91	24.587716086969063	23.910952960377664	25.764743481101675	25.736587471551598
92-93	24.54923918069642	24.10401540830833	25.77360276109768	25.573142649897562
94-95	24.55330464578668	23.897566399749252	25.86432906789775	25.684799886566317
96-97	24.322293241531572	23.947217431313	25.90095403913113	25.829535288024296
98	24.520800839943952	23.794274007029767	25.84701906578623	25.837906087240047
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	74.5
2	124.0
3	201.5
4	232.0
5	279.5
6	412.0
7	729.0
8	1060.5
9	1262.5
10	1364.5
11	1515.5
12	1762.0
13	2009.0
14	2294.0
15	2480.0
16	2569.0
17	2611.5
18	2517.0
19	2450.0
20	2315.5
21	2104.0
22	2115.0
23	2333.5
24	2583.5
25	3059.0
26	3823.0
27	4782.5
28	6382.0
29	8741.0
30	12354.5
31	17814.5
32	25263.5
33	34336.5
34	46102.0
35	60470.5
36	76519.0
37	94997.5
38	114339.5
39	133317.0
40	151591.0
41	172318.5
42	198749.0
43	227983.5
44	254212.0
45	277144.5
46	300395.0
47	323688.5
48	338331.5
49	344744.5
50	347992.0
51	348497.0
52	351720.0
53	356767.0
54	355619.5
55	342004.5
56	317888.0
57	294112.0
58	269276.0
59	237845.0
60	201480.0
61	163789.5
62	129449.0
63	97358.5
64	71072.5
65	52063.5
66	37692.0
67	27101.5
68	19289.0
69	13753.5
70	9730.5
71	6718.5
72	4713.5
73	3293.5
74	2310.5
75	1541.0
76	982.0
77	599.5
78	355.5
79	210.0
80	136.0
81	90.0
82	58.5
83	41.0
84	36.5
85	30.5
86	25.0
87	24.5
88	20.0
89	18.5
90	16.0
91	16.0
92	15.5
93	9.5
94	8.5
95	8.5
96	7.5
97	9.5
98	6.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27064225041986834
2	0.13592942261555288
3	0.13389699363677976
4	0.12850103782693836
5	0.12449343138992092
6	0.04472775041314128
7	0.034808924481523076
8	0.01362586188585936
9	0.009088678883950308
10-11	0.0018248922168561644
12-13	0.00560349257175834
14-15	0.01041262029617929
16-17	0.0057680906932787
18-19	0.04804118216374679
20-21	0.09337007354244069
22-23	0.09259717801530161
24-25	0.07060543769216832
26-27	0.08104668374861201
28-29	0.09844398954930744
30-31	0.07373280200105514
32-33	0.019071912776163446
34-35	0.2878534887788451
36-37	0.37323697520751525
38-39	0.346085441596722
40-41	0.3428006356063809
42-43	0.32047254260014074
44-45	0.30521501237921
46-47	0.3099812014632343
48-49	0.3037765679259234
50-51	0.30580184046463044
52-53	0.3489623305032953
54-55	0.33604495618398006
56-57	0.3211023093259578
58-59	0.30327561712129625
60-61	0.31083281783110056
62-63	0.3101171738244903
64-65	0.3266342374970551
66-67	0.34569899383315245
68-69	0.30910811577516983
70-71	0.2864866087262195
72-73	0.27162268270892437
74-75	0.25836179926643627
76-77	0.26214039962133845
78-79	0.26790849031461716
80-81	0.258762559910138
82-83	0.2679299596348155
84-85	0.26121721885281124
86-87	0.2563436831677953
88-89	0.27924429137932366
90-91	0.26331405579217926
92-93	0.25400352726617975
94-95	0.268595508560963
96-97	0.2670210917464204
98	0.26664895686298307
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	6986714.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.81264555578385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.56980726454641	39.67284284072626
2	11.31686991961613	11.727139398919652
3	4.473802081902272	6.953985646689907
4	2.298709374426791	4.764088562117317
5	1.303652923359918	3.377285342290443
6	0.8799979037916837	2.735701168739477
7	0.599996399697878	2.1761180554604795
8	0.42302723079147225	1.753452797555466
9	0.30882727170435736	1.4401042170099858
>10	1.6643702879838222	15.496145947197085
>50	0.10857931448571582	3.865153939092795
>100	0.04912913639677001	4.5046740044644284
>500	0.0023953723890575364	0.8179190485467422
>1k	8.355189077445326E-4	0.7153890311901543
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.2938640396615634E-5
2	0.0	0.0	0.0	0.0	4.2938640396615634E-5
3	0.0	0.0	0.0	0.0	4.2938640396615634E-5
4	0.0	0.0	0.0	0.0	5.725152052882084E-5
5	1.431288013220521E-5	1.431288013220521E-5	0.0	0.0	5.725152052882084E-5
6	1.431288013220521E-5	1.431288013220521E-5	0.0	1.431288013220521E-5	7.156440066102605E-5
7	1.431288013220521E-5	1.431288013220521E-5	0.0	1.431288013220521E-5	7.156440066102605E-5
8	1.431288013220521E-5	1.431288013220521E-5	0.0	1.431288013220521E-5	8.587728079323127E-5
9	1.431288013220521E-5	1.431288013220521E-5	0.0	1.431288013220521E-5	1.1450304105764169E-4
10-11	1.0019016092543648E-4	1.431288013220521E-5	0.0	2.862576026441042E-5	1.1450304105764169E-4
12-13	1.1450304105764169E-4	1.431288013220521E-5	0.0	4.2938640396615634E-5	1.1450304105764169E-4
14-15	1.2165948112374429E-4	1.431288013220521E-5	0.0	7.156440066102605E-5	1.7891100165256515E-4
16-17	1.288159211898469E-4	1.431288013220521E-5	0.0	9.303372085933388E-5	2.36162522181386E-4
18-19	1.288159211898469E-4	1.431288013220521E-5	0.0	1.1450304105764169E-4	2.576318423796938E-4
20-21	1.288159211898469E-4	1.431288013220521E-5	0.0	1.6459812152035992E-4	2.647882824457964E-4
22-23	1.5028524138815472E-4	1.431288013220521E-5	0.0	2.433189622474886E-4	3.792913235034381E-4
24-25	2.504754023135912E-4	2.1469320198307817E-5	0.0	4.8663792449497715E-4	4.794814844288746E-4
26-27	3.0057048277630944E-4	4.2938640396615634E-5	0.0	9.661194089238517E-4	5.009508046271824E-4
28-29	3.0057048277630944E-4	4.2938640396615634E-5	0.0	0.003284805990341096	5.009508046271824E-4
30-31	3.0772692284241205E-4	4.2938640396615634E-5	0.0	0.012910217879249102	5.009508046271824E-4
32-33	3.578220033051303E-4	4.2938640396615634E-5	0.0	0.033950151673590764	5.152636847593876E-4
34-35	3.7929132350343815E-4	4.2938640396615634E-5	0.0	0.06211074333370452	5.582023251560032E-4
36-37	4.0076064370174594E-4	5.0095080462718235E-5	0.0	0.1101232997371869	5.79671645354311E-4
38-39	4.0076064370174594E-4	5.725152052882084E-5	0.0	0.18666142624415427	6.011409655526189E-4
40-41	4.4369928409836157E-4	5.725152052882084E-5	0.0	0.27323288172379745	6.154538456848241E-4
42-43	4.4369928409836157E-4	5.725152052882084E-5	0.0	0.3625738795090224	6.226102857509267E-4
44-45	4.72325044362772E-4	6.440796059492345E-5	0.0	0.4689615175317038	6.369231658831318E-4
46-47	5.009508046271824E-4	7.156440066102605E-5	0.0	0.5967898499924285	6.727053662136449E-4
48-49	5.009508046271824E-4	7.156440066102605E-5	0.0	0.7370489188479734	7.585826470068762E-4
50-51	5.009508046271824E-4	7.156440066102605E-5	0.0	0.8913847053135422	7.728955271390814E-4
52-53	5.009508046271824E-4	7.156440066102605E-5	0.0	1.0490625492899808	8.587728079323127E-4
54-55	5.08107244693285E-4	7.156440066102605E-5	0.0	1.224194950587644	9.518065287916466E-4
56-57	5.152636847593876E-4	7.156440066102605E-5	0.0	1.427516569305685	9.661194089238518E-4
58-59	5.152636847593876E-4	7.156440066102605E-5	0.0	1.653345192031619	9.732758489899544E-4
60-61	5.295765648915928E-4	7.156440066102605E-5	0.0	1.8986965832578806	9.875887291221595E-4
62-63	5.295765648915928E-4	7.872084072712865E-5	0.0	2.1501166356601975	0.0010305273695187752
64-65	5.438894450237981E-4	8.587728079323127E-5	0.0	2.4187264571012923	0.0010376838095848778
66-67	5.438894450237981E-4	8.587728079323127E-5	0.0	2.7254801040947143	0.0010448402496509804
68-69	5.868280854204136E-4	8.587728079323127E-5	0.0	3.070198665638811	0.0010448402496509804
70-71	6.082974056187215E-4	8.587728079323127E-5	0.0	3.4472714927217574	0.0011092482102459038
72-73	6.154538456848241E-4	8.587728079323127E-5	0.0	3.833962575253546	0.0012022819311052378
74-75	6.154538456848241E-4	8.587728079323127E-5	0.0	4.229470391946772	0.0012022819311052378
76-77	6.154538456848241E-4	8.587728079323127E-5	0.0	4.665784229896916	0.0012237512513035456
78-79	6.297667258170293E-4	1.0019016092543648E-4	0.0	5.150697452335962	0.0012452205715018534
80-81	6.297667258170293E-4	1.0019016092543648E-4	0.0	5.672158614192595	0.0012953156519645717
82-83	6.297667258170293E-4	1.0019016092543648E-4	0.0	6.207725119419516	0.0013883493728239055
84-85	6.297667258170293E-4	1.0019016092543648E-4	0.0	6.749396068022822	0.0014026622529561108
86	6.297667258170293E-4	1.0019016092543648E-4	0.0	7.169951424947407	0.0014026622529561108
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10800	0.0	34.641308	1
GGTATCA	7195	0.0	30.444134	1
TCAACGC	13235	0.0	27.393925	4
TATCAAC	13360	0.0	27.207582	2
CAACGCA	13425	0.0	27.00526	5
GTACATG	23575	0.0	26.371275	1
ATCAACG	14025	0.0	26.015263	3
AACGCAG	14385	0.0	25.367958	6
TACATGG	24380	0.0	24.842861	2
ACATGGG	26450	0.0	22.429062	3
ACGCAGA	17395	0.0	20.524885	7
GAGTACA	18015	0.0	20.17996	1
AGTACAT	16885	0.0	20.00351	2
CGCAGAG	18140	0.0	19.22451	8
CATGGGG	21755	0.0	19.0946	4
GTATATA	1235	0.0	19.003374	1
TACACTG	7100	0.0	16.69733	5
ACCGCTC	3150	0.0	16.606316	8
ATTCACG	1975	0.0	16.49379	9
ATGGGGG	11590	0.0	15.898151	5
>>END_MODULE