FastQCFastQC Report
Mon 15 Aug 2016
SRR2921574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6986714
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111600.033.556951
GGTATCA75900.032.1623341
TCAACGC139850.026.3865624
TATCAAC142700.025.758252
CAACGCA142850.025.6665525
GTACATG240200.025.6548041
ATCAACG147350.025.0112763
TACATGG247650.024.3345132
AACGCAG154550.023.6588176
ACATGGG261750.022.2266313
GTATATA13500.020.4440161
GAGTACA187150.020.086731
ACGCAGA182800.019.638897
AGTACAT179250.019.6317212
CATGGGG217350.018.6386154
CGCAGAG192100.018.4653848
GTACCGT10400.017.8074866
ACCGTAT6850.017.3306378
ATTCACG23800.017.1563289
ATGGGGG111050.016.3798795
Produced by FastQC (version 0.11.5)