##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921574_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6986714
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.04105892984885	31.0	30.0	34.0	26.0	34.0
2	30.026580879079923	31.0	30.0	34.0	26.0	34.0
3	30.20836862651026	31.0	30.0	34.0	26.0	34.0
4	33.85983453738052	35.0	35.0	37.0	30.0	37.0
5	33.37348845251144	35.0	33.0	37.0	28.0	37.0
6	33.26414477535505	35.0	33.0	37.0	27.0	37.0
7	33.07511084610018	35.0	33.0	37.0	26.0	37.0
8	32.97322002875744	35.0	33.0	37.0	26.0	37.0
9	34.29321709747959	37.0	34.0	39.0	27.0	39.0
10-11	34.079705853137824	37.0	33.5	39.0	26.0	39.0
12-13	33.89311942638557	37.0	33.0	39.0	25.0	39.0
14-15	34.76265444671129	38.0	33.0	40.0	25.0	41.0
16-17	34.61033477254114	38.0	33.0	40.0	25.0	41.0
18-19	34.466290805663434	38.0	33.0	40.0	24.0	41.0
20-21	34.318504593146365	38.0	33.0	40.0	23.0	41.0
22-23	34.144999852577335	38.0	33.0	40.0	22.0	41.0
24-25	33.98659834938141	38.0	33.0	40.0	21.5	41.0
26-27	33.896085913921766	38.0	32.5	40.0	20.0	41.0
28-29	33.908802263839625	38.0	33.0	40.0	20.0	41.0
30-31	33.757068845239694	38.0	32.5	40.0	19.0	41.0
32-33	33.58806142630141	38.0	32.0	40.0	18.0	41.0
34-35	33.38360522557528	38.0	32.0	40.0	17.5	41.0
36-37	33.21643357664276	38.0	32.0	40.0	16.0	41.0
38-39	33.03520209929876	38.0	31.5	40.0	15.5	41.0
40-41	33.04935596333269	38.0	32.0	40.0	15.5	41.0
42-43	32.844150411767245	37.5	31.0	40.0	14.5	41.0
44-45	32.64060250641432	37.0	31.0	40.0	11.0	41.0
46-47	32.37122465582533	37.0	31.0	40.0	9.0	41.0
48-49	32.07882697073331	37.0	30.5	40.0	8.0	41.0
50-51	31.79709846431384	36.5	30.0	40.0	7.0	41.0
52-53	31.459909909007294	36.0	30.0	39.0	4.0	41.0
54-55	31.13040429306252	36.0	29.0	39.0	2.0	40.5
56-57	30.76890724309024	35.0	29.0	39.0	2.0	40.0
58-59	30.275669578001903	35.0	28.0	39.0	2.0	40.0
60-61	29.898115838146516	34.5	28.0	38.0	2.0	40.0
62-63	29.544924695643758	34.0	27.5	38.0	2.0	40.0
64-65	29.07350179211572	34.0	26.5	37.5	2.0	40.0
66-67	28.631633769465875	34.0	26.0	37.0	2.0	39.0
68-69	28.18513646043047	33.5	26.0	36.0	2.0	39.0
70-71	27.70503973684911	33.0	25.5	36.0	2.0	39.0
72-73	27.214157685572932	33.0	25.0	35.0	2.0	38.0
74-75	26.669692791203417	32.0	23.5	35.0	2.0	37.0
76-77	25.211550666021253	30.5	21.0	34.0	2.0	35.5
78-79	26.081682104062082	32.0	23.0	34.5	2.0	36.0
80-81	26.132101872210598	32.0	23.0	35.0	2.0	36.0
82-83	25.80632812506709	32.0	22.0	35.0	2.0	35.5
84-85	25.37645408127483	32.0	20.0	34.5	2.0	35.0
86-87	24.923639496335472	31.5	18.5	34.0	2.0	35.0
88-89	24.47424218309208	31.0	16.5	34.0	2.0	35.0
90-91	24.036336466613633	31.0	9.0	34.0	2.0	35.0
92-93	23.61421384931457	31.0	4.5	34.0	2.0	35.0
94-95	23.21647443991553	31.0	2.0	34.0	2.0	35.0
96-97	22.657169522038544	30.5	2.0	34.0	2.0	35.0
98-99	21.873412594246737	30.0	2.0	34.0	2.0	35.0
100-101	19.296409299135476	25.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	184672.0
3	83470.0
4	44692.0
5	31716.0
6	27579.0
7	26959.0
8	31062.0
9	35347.0
10	40404.0
11	45157.0
12	50214.0
13	54832.0
14	58732.0
15	62501.0
16	65654.0
17	67756.0
18	68281.0
19	67272.0
20	68110.0
21	70798.0
22	75927.0
23	85530.0
24	98104.0
25	114345.0
26	136367.0
27	159827.0
28	188809.0
29	220158.0
30	258506.0
31	303937.0
32	361189.0
33	436526.0
34	540606.0
35	682383.0
36	863165.0
37	888236.0
38	372610.0
39	15281.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31547357760315	17.92308972339614	13.31578917168031	25.445647527320403
2	16.48782245845472	19.52843640086026	37.35978029156482	26.623960849120206
3	20.553459195112094	22.37599030845983	27.90502886049976	29.165521635928314
4	14.698297685762476	15.168963860195541	33.72948690659094	36.403251547451035
5	15.592076501402435	36.20171601937787	32.9630866017474	15.243120877472299
6	36.12358686049267	33.0422399437882	16.012424136570363	14.82174905914876
7	31.55902364523208	30.326784514448335	18.844910003187554	19.269281837132027
8	28.166967221809696	34.33268157305497	18.345795142761304	19.15455606237403
9	27.26554994272139	15.72892852818688	18.02985938620542	38.975662142886314
10-11	27.07522942668728	24.559571407260687	26.100453501124115	22.26474566492791
12-13	27.237871979402183	21.352639868610424	26.578502950792803	24.830985201194586
14-15	24.35889820948258	23.025197271596596	23.47743541658263	29.1384691023382
16-17	24.987717957906757	26.280003632333383	24.227627848538575	24.50465056122129
18-19	24.983907188523528	25.727807604745077	25.50703457111189	23.7812506356195
20-21	25.486819696687085	24.682128624981697	25.31609206718695	24.514959611144267
22-23	25.313308104721422	24.46608597600393	25.146646857350436	25.073959061924217
24-25	24.981326130483474	24.79376385142776	25.177353419613414	25.047556598475353
26-27	25.002590116544866	24.838827447259177	25.268854813353016	24.889727622842944
28-29	25.047117239487708	25.15111837727304	25.046716409225077	24.755047974014175
30-31	25.322629347032876	24.890082600729638	24.898536959291512	24.888751092945974
32-33	25.002382189029476	24.85103891004203	25.043117442456087	25.103461458472413
34-35	25.17075134889234	24.904287745752857	25.241186862022712	24.68377404333209
36-37	25.139379565939418	24.997259908375344	25.093032464233307	24.77032806145193
38-39	25.30343579134139	24.894428012669355	25.039321475632427	24.762814720356833
40-41	25.223542003076933	24.796103079815012	25.079781083127294	24.90057383398076
42-43	25.071943620254512	24.71726515168435	25.147194499469975	25.063596728591154
44-45	24.97020302312984	24.77805797290658	25.185259464888382	25.0664795390752
46-47	25.104392338233755	24.761643579087345	25.085558336869628	25.048405745809273
48-49	24.838464233696996	24.82498145043832	25.196266099187483	25.140288216677202
50-51	24.92576517732256	24.849501181215135	25.134616152678586	25.09011748878372
52-53	24.925842761605107	24.914448820233233	25.12595243544781	25.033755982713846
54-55	24.677370244869508	24.61256586548489	25.336486755184456	25.373577134461144
56-57	24.837807828024122	24.740191208048437	25.159014389200806	25.262986574726636
58-59	24.888079260324005	24.851958018915592	25.20365165925825	25.056311061502157
60-61	25.124667816758194	24.624299975023845	25.185937786677275	25.065094421540685
62-63	24.85863878392637	24.68987565824347	25.26129690872677	25.19018864910339
64-65	24.734306978623096	24.556766745057306	25.312570657326876	25.396355618992715
66-67	24.707734746655234	24.879724141012545	25.087609402554715	25.32493170977751
68-69	24.710048533057783	24.662644184347986	25.058028967430296	25.569278315163935
70-71	24.706447723408807	24.4803180648089	25.18962555382545	25.623608657956844
72-73	24.724098228248355	24.64743259266897	25.02786799791491	25.600601181167765
74-75	24.502871149697793	24.637638304945217	25.168330069342478	25.691160476014517
76-77	24.47219718262795	24.298960876158805	25.376096458755608	25.852745482457628
78-79	24.461994972038113	24.264925736832467	25.412119775869463	25.860959515259957
80-81	24.54690667307788	24.449958268167233	25.18104729901171	25.822087759743173
82-83	24.360582422723766	24.518504835116907	25.240088230425155	25.880824511734176
84-85	24.50025783445968	24.44546264320836	25.16610351573964	25.888176006592317
86-87	24.240490967123055	24.52964682670476	25.24503840859763	25.984823797574553
88-89	24.216851380684094	24.317038947728122	25.27539728245804	26.190712389129743
90-91	24.266401860379936	24.137268609647872	25.28630696699542	26.31002256297677
92-93	24.250847915937378	24.30736129249214	25.284981708324384	26.156809083246106
94-95	24.167749577797057	24.120394431086275	25.328433741941144	26.383422249175524
96-97	23.90100126089795	24.16910489414197	25.39567132259449	26.534222522365585
98-99	23.970143545381703	24.01891714171124	25.412718379036185	26.598220933870877
100-101	24.239812228560602	23.66852094493761	25.422208054945877	26.669458771555917
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	56.0
2	65.5
3	106.5
4	103.5
5	109.5
6	210.0
7	434.5
8	654.0
9	709.5
10	643.0
11	585.0
12	569.0
13	601.5
14	663.5
15	718.5
16	818.0
17	924.5
18	1060.5
19	1177.0
20	1238.5
21	1356.5
22	1544.5
23	1774.0
24	2077.0
25	2485.0
26	3004.5
27	3736.5
28	4920.0
29	6679.5
30	9353.5
31	13434.5
32	19308.5
33	27149.0
34	36707.5
35	48762.5
36	63441.5
37	79342.5
38	97439.0
39	115858.0
40	133299.5
41	151211.0
42	172020.0
43	199307.0
44	227639.5
45	254675.0
46	275508.5
47	295280.5
48	319909.5
49	334744.5
50	340500.5
51	345153.5
52	349855.0
53	354355.0
54	355290.5
55	343829.0
56	321504.0
57	297167.0
58	272309.0
59	243875.5
60	205418.0
61	165496.5
62	130778.5
63	98230.5
64	71332.0
65	51492.0
66	37278.5
67	26994.5
68	19081.5
69	13358.0
70	9567.0
71	6704.5
72	4571.5
73	3236.5
74	2329.0
75	1510.5
76	913.0
77	505.5
78	270.0
79	153.0
80	86.5
81	57.5
82	30.0
83	12.5
84	4.0
85	2.5
86	3.0
87	3.0
88	2.5
89	1.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22519885600011677
2	0.0
3	2.8625760264410425E-5
4	0.012466518595150737
5	0.014484634693791673
6	0.10585806145778974
7	0.1370887659062615
8	0.15363445533909073
9	0.10976547773388176
10-11	0.11938373318272366
12-13	0.09383524214673736
14-15	0.11737277352414884
16-17	0.07122089153785313
18-19	0.076373528385447
20-21	0.052406610604069384
22-23	0.07853477328540999
24-25	0.07203672570538883
26-27	0.04920052545445542
28-29	0.017368680040431024
30-31	0.030787005164373406
32-33	0.0370918288626098
34-35	0.04323921087939194
36-37	0.03518821580502651
38-39	0.037456807305981034
40-41	0.021548041039034946
42-43	0.029742164914722428
44-45	0.015830045426218964
46-47	0.028711637545203655
48-49	3.721348834373355E-4
50-51	0.01590160982687999
52-53	0.00780051967205184
54-55	0.015386346142120602
56-57	0.010176457773997906
58-59	0.007442697668746711
60-61	0.029377186471351197
62-63	0.01269552467726602
64-65	0.013933588808701772
66-67	0.057480526610936125
68-69	0.07567219725896895
70-71	0.0318533147342227
72-73	0.054303067221586565
74-75	0.04562230542140411
76-77	0.19849817811348797
78-79	0.07381867928184838
80-81	0.014878238897427317
82-83	0.009854417971023289
84-85	0.0230723627731148
86-87	0.16578609057133295
88-89	0.3759922046329648
90-91	0.2734475749257806
92-93	0.2767681631164522
94-95	0.0020753676191697557
96-97	0.006054348295922805
98-99	0.005038133806536235
100-101	0.004980882286007414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6986714.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.59232901720326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.06983044099334	33.579012521208135
2	13.128185015412141	12.233454312736006
3	5.484477009071573	7.666036718818489
4	2.773751445311175	5.169421598075254
5	1.6347948253805082	3.8084449189875005
6	1.070478805082862	2.9925660415417985
7	0.7095700554628204	2.3142365039415225
8	0.5104606684378752	1.9026841131359191
9	0.3812489967436754	1.5986950824383985
>10	2.0288457970834366	17.101875762479782
>50	0.13833348131957965	4.404476908806788
>100	0.06569132376821117	5.399457671295851
>500	0.0031563517728422376	0.9524687501593696
>1k	0.0011757841598711027	0.8771690963752077
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.431288013220521E-5
2	0.0	0.0	0.0	0.0	1.431288013220521E-5
3	0.0	0.0	0.0	0.0	1.431288013220521E-5
4	0.0	0.0	0.0	0.0	1.431288013220521E-5
5	2.862576026441042E-5	0.0	0.0	0.0	1.431288013220521E-5
6	2.862576026441042E-5	0.0	0.0	0.0	2.862576026441042E-5
7	2.862576026441042E-5	0.0	0.0	0.0	2.862576026441042E-5
8	2.862576026441042E-5	0.0	0.0	0.0	2.862576026441042E-5
9	2.862576026441042E-5	0.0	0.0	0.0	5.725152052882084E-5
10-11	1.0019016092543648E-4	0.0	0.0	5.0095080462718235E-5	5.725152052882084E-5
12-13	1.5028524138815472E-4	0.0	0.0	7.872084072712865E-5	8.587728079323127E-5
14-15	1.6459812152035992E-4	0.0	0.0	9.303372085933388E-5	2.1469320198307817E-4
16-17	1.8606744171866777E-4	0.0	0.0	1.0734660099153909E-4	3.435091231729251E-4
18-19	2.0038032185087297E-4	0.0	0.0	1.2165948112374429E-4	3.578220033051303E-4
20-21	2.0038032185087297E-4	0.0	0.0	1.7891100165256515E-4	3.649784433712329E-4
22-23	2.0038032185087297E-4	0.0	0.0	2.36162522181386E-4	4.3654284403225896E-4
24-25	2.290060821152834E-4	0.0	0.0	4.580121642305668E-4	5.224201248254902E-4
26-27	2.576318423796938E-4	0.0	0.0	9.303372085933386E-4	5.582023251560032E-4
28-29	2.576318423796938E-4	0.0	0.0	0.003249023790010583	5.653587652221058E-4
30-31	2.7910116257800165E-4	0.0	0.0	0.012953156519645716	5.725152052882084E-4
32-33	3.2919624304071984E-4	0.0	0.0	0.03400024675405348	6.082974056187215E-4
34-35	3.578220033051303E-4	0.0	0.0	0.06204633537310959	6.297667258170293E-4
36-37	3.864477635695407E-4	1.431288013220521E-5	0.0	0.11047396530042591	6.369231658831318E-4
38-39	3.864477635695407E-4	1.431288013220521E-5	0.0	0.18850063134114264	6.440796059492345E-4
40-41	4.0076064370174594E-4	1.431288013220521E-5	0.0	0.277383616962137	6.440796059492345E-4
42-43	4.0076064370174594E-4	1.431288013220521E-5	0.0	0.3685924456046147	6.440796059492345E-4
44-45	4.222299639000537E-4	1.431288013220521E-5	0.0	0.4767835065239539	6.440796059492345E-4
46-47	4.72325044362772E-4	1.431288013220521E-5	0.0	0.606379479681006	6.583924860814397E-4
48-49	4.866379244949772E-4	1.431288013220521E-5	0.0	0.7466600178567493	6.941746864119528E-4
50-51	5.009508046271824E-4	1.431288013220521E-5	0.0	0.9000797799938569	7.013311264780554E-4
52-53	5.152636847593876E-4	1.431288013220521E-5	0.0	1.056612593559719	7.228004466763632E-4
54-55	5.367330049576955E-4	1.431288013220521E-5	0.0	1.2299344155206582	7.44269766874671E-4
56-57	5.438894450237981E-4	1.431288013220521E-5	0.0	1.431209292379794	7.44269766874671E-4
58-59	5.438894450237981E-4	1.431288013220521E-5	0.0	1.6535956674339325	7.44269766874671E-4
60-61	5.582023251560032E-4	1.431288013220521E-5	0.0	1.8959127280721666	7.728955271390814E-4
62-63	5.582023251560032E-4	1.431288013220521E-5	0.0	2.1459730568619237	8.730856880645179E-4
64-65	5.582023251560032E-4	2.1469320198307817E-5	0.0	2.412092437160015	8.730856880645179E-4
66-67	5.582023251560032E-4	2.862576026441042E-5	0.0	2.7153036463207165	8.730856880645179E-4
68-69	5.868280854204136E-4	2.862576026441042E-5	0.0	3.0556496229844243	8.730856880645179E-4
70-71	6.011409655526189E-4	2.862576026441042E-5	0.0	3.4292802023955753	9.160243284611335E-4
72-73	6.011409655526189E-4	2.862576026441042E-5	0.0	3.8156707144445874	0.0010591531297831857
74-75	6.011409655526189E-4	2.862576026441042E-5	0.0	4.212674227111629	0.0010591531297831857
76-77	6.154538456848241E-4	2.862576026441042E-5	0.0	4.65000427955116	0.0010663095698492883
78-79	6.440796059492345E-4	2.862576026441042E-5	0.0	5.136906992328582	0.001073466009915391
80-81	6.440796059492345E-4	2.862576026441042E-5	0.0	5.6600785433610135	0.0011020917701798014
82-83	6.512360460153371E-4	2.862576026441042E-5	0.0	6.2000247899083885	0.0011664997307747247
84-85	6.583924860814397E-4	2.862576026441042E-5	0.0	6.745302584305011	0.0011736561708408273
86-87	6.583924860814397E-4	2.862576026441042E-5	0.0	7.321832552470303	0.0011736561708408273
88-89	6.583924860814397E-4	4.2938640396615634E-5	0.0	7.9575176542219985	0.0012022819311052378
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11160	0.0	33.55695	1
GGTATCA	7590	0.0	32.162334	1
TCAACGC	13985	0.0	26.386562	4
TATCAAC	14270	0.0	25.75825	2
CAACGCA	14285	0.0	25.666552	5
GTACATG	24020	0.0	25.654804	1
ATCAACG	14735	0.0	25.011276	3
TACATGG	24765	0.0	24.334513	2
AACGCAG	15455	0.0	23.658817	6
ACATGGG	26175	0.0	22.226631	3
GTATATA	1350	0.0	20.444016	1
GAGTACA	18715	0.0	20.08673	1
ACGCAGA	18280	0.0	19.63889	7
AGTACAT	17925	0.0	19.631721	2
CATGGGG	21735	0.0	18.638615	4
CGCAGAG	19210	0.0	18.465384	8
GTACCGT	1040	0.0	17.807486	6
ACCGTAT	685	0.0	17.330637	8
ATTCACG	2380	0.0	17.156328	9
ATGGGGG	11105	0.0	16.379879	5
>>END_MODULE