FastQCFastQC Report
Mon 15 Aug 2016
SRR2921573_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921573_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2936580
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33760.11496366521599956No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39000.043.1899641
GTATCAA65950.038.799521
TATCAAC84850.029.7316822
TCAACGC85200.029.4462414
CAACGCA87600.028.6904775
ATCAACG89200.028.1262593
GTACATG111650.027.617381
AACGCAG92900.027.220396
TACATGG115250.026.3149172
ACATGGG123250.023.8603743
CATGGGG89650.022.9104714
ACGCAGA106850.022.7166067
CGCAGAG110750.021.7472278
GTGGTAT20000.019.78951
GTATATA6850.018.811951
ATGGGGG52850.018.7786055
GCAGAGT118750.018.6966199
GAGTACA88350.017.9712751
AGAGTAC99200.017.52643810-11
AGTACAT83500.017.4450322
Produced by FastQC (version 0.11.5)