##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921573_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2936580
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.141257517247954	31.0	28.0	31.0	16.0	34.0
2	28.106173507958236	31.0	28.0	33.0	16.0	34.0
3	28.190765788774698	31.0	28.0	34.0	16.0	34.0
4	31.61594099258321	35.0	32.0	37.0	18.0	37.0
5	31.562939201383923	35.0	32.0	37.0	17.0	37.0
6	31.649830755504702	35.0	32.0	37.0	18.0	37.0
7	31.46374251680526	35.0	32.0	37.0	17.0	37.0
8	31.416647256332197	35.0	32.0	37.0	17.0	37.0
9	32.65620619904787	37.0	32.0	39.0	17.0	39.0
10-11	32.42106038316682	37.0	32.0	39.0	16.0	39.0
12-13	32.22952601325351	37.0	32.0	39.0	14.0	39.0
14-15	32.932004236220365	38.0	32.0	40.0	11.0	41.0
16-17	32.7902267263279	38.0	32.0	40.0	10.0	41.0
18-19	32.62142849845739	38.0	31.0	40.0	10.0	41.0
20-21	32.49661698302107	37.5	31.0	40.0	10.0	41.0
22-23	32.270109106511654	37.0	31.0	40.0	9.5	41.0
24-25	32.3650743381757	37.5	31.0	40.0	8.0	41.0
26-27	32.120003711800805	37.0	30.5	40.0	8.0	41.0
28-29	31.92147549189874	37.0	30.0	40.0	2.0	41.0
30-31	31.65784075352962	37.0	30.0	40.0	2.0	41.0
32-33	31.405247600950766	37.0	30.0	40.0	2.0	41.0
34-35	31.061720947496745	36.5	29.5	40.0	2.0	41.0
36-37	30.750666591749585	36.0	29.0	40.0	2.0	41.0
38-39	30.523625271574417	36.0	28.5	39.0	2.0	41.0
40-41	30.285247464737893	36.0	28.0	39.0	2.0	40.0
42-43	30.022135613536836	35.0	27.0	39.0	2.0	40.0
44-45	29.740914771605063	35.0	27.0	39.0	2.0	40.0
46-47	29.43435492988442	35.0	26.0	39.0	2.0	40.0
48-49	29.1261383990901	35.0	26.0	39.0	2.0	40.0
50-51	28.183699405430808	34.0	24.0	38.0	2.0	39.5
52-53	28.374701353274897	33.5	25.0	38.0	2.0	39.5
54-55	28.93956864788291	35.0	26.0	39.0	2.0	40.0
56-57	28.916370063134668	35.0	26.0	39.0	2.0	40.0
58-59	28.663910228905735	34.5	26.0	39.0	2.0	40.0
60-61	28.312607863569184	34.0	25.0	38.0	2.0	40.0
62-63	27.8883636747509	34.0	23.5	38.0	2.0	40.0
64-65	27.417035633287703	34.0	22.5	37.5	2.0	40.0
66-67	27.065129844921643	33.0	22.0	37.0	2.0	39.5
68-69	26.606438441997156	33.0	20.5	36.0	2.0	39.0
70-71	26.178933316987788	33.0	20.0	36.0	2.0	39.0
72-73	25.704730843362007	32.0	18.0	35.0	2.0	38.5
74-75	25.26426897956126	32.0	15.5	35.0	2.0	37.0
76-77	24.84367699841312	32.0	11.0	35.0	2.0	37.0
78-79	24.44612031683114	31.0	7.0	35.0	2.0	36.0
80-81	24.00307108949867	31.0	3.5	34.5	2.0	36.0
82-83	23.466816841359677	31.0	2.0	34.0	2.0	35.0
84-85	23.047071082688024	30.5	2.0	34.0	2.0	35.0
86-87	22.548904507965048	30.0	2.0	34.0	2.0	35.0
88-89	22.035817515613402	30.0	2.0	34.0	2.0	35.0
90-91	21.4378520251449	29.0	2.0	34.0	2.0	35.0
92-93	20.885626987856625	29.0	2.0	34.0	2.0	35.0
94-95	20.206884028359518	29.0	2.0	34.0	2.0	35.0
96-97	19.567322531652465	27.0	2.0	34.0	2.0	35.0
98	19.09442412602415	26.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	183581.0
3	30389.0
4	22185.0
5	18026.0
6	16681.0
7	17551.0
8	20233.0
9	22489.0
10	23692.0
11	25367.0
12	25618.0
13	25442.0
14	24955.0
15	26587.0
16	28549.0
17	30340.0
18	31109.0
19	30945.0
20	31267.0
21	33843.0
22	36442.0
23	40281.0
24	44862.0
25	51818.0
26	59384.0
27	67976.0
28	78269.0
29	89844.0
30	103582.0
31	120810.0
32	142969.0
33	171459.0
34	210020.0
35	257461.0
36	313845.0
37	313871.0
38	152818.0
39	12020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.080985790432784	17.56798107212428	12.413802759950931	24.937230377492
2	16.493125889187617	20.145939302672303	38.43152419886157	24.929410609278516
3	19.637165428653365	24.34168416867262	28.760851158440126	27.260299244233888
4	14.027282166342447	15.844456530449058	35.517929815169374	34.61033148803912
5	14.876712179300958	36.791788904300724	33.32324063049387	15.00825828590445
6	33.15628521940111	34.01425878960415	17.774494987005873	15.054961003988861
7	29.134362545831	31.15165901155948	21.159197727961008	18.554780714648516
8	27.124275163154277	33.15739032170393	20.342227208295956	19.37610730684584
9	27.722351125373894	15.162790887756175	19.164510050529355	37.95034793634057
10-11	26.257957960514556	24.554873532491637	27.225921795319298	21.961246711674512
12-13	26.942037166163075	22.232592036357353	27.126054541135723	23.69931625634385
14-15	24.453984953264392	23.421001232353564	24.649608522975637	27.475405291406407
16-17	24.363117979168678	26.14142066353119	25.203433937969187	24.292027419330946
18-19	24.333617253245205	25.72144049606487	26.494122857824266	23.45081939286566
20-21	25.145226956959238	24.971145494879476	26.15390751014765	23.72972003801364
22-23	25.033486048005503	24.64227151897213	25.976361807140474	24.347880625881892
24-25	24.60205960999397	24.92020063450996	25.944825680154022	24.532914075342052
26-27	24.409028562021902	25.09396018559086	26.06013758617029	24.43687366621695
28-29	24.487628497679918	25.256228362142902	25.889938899259455	24.366204240917725
30-31	24.843197690190536	25.17005944923913	25.60831185154185	24.378431009028485
32-33	24.643009560957534	25.211997399185222	25.63582189800215	24.5091711418551
34-35	24.8143720347175	25.16854930438614	25.758907375590496	24.258171285305863
36-37	24.885770321463657	24.98274738253391	25.773760875730385	24.357721420272053
38-39	24.88526105246752	25.08678051468125	25.648034091514994	24.379924341336235
40-41	24.867045205399158	24.88770551151688	25.693628337669033	24.551620945414925
42-43	24.60868140509938	24.915503817298607	25.627077594685865	24.848737182916146
44-45	24.38621430408924	24.958944300560177	25.81358953046355	24.841251864887035
46-47	24.58732431250044	24.874584275079428	25.67985512640993	24.858236286010204
48-49	24.550147855413258	25.032539806175492	25.53682993621173	24.88048240219952
50-51	24.387605410923243	25.09225804512018	25.71871084029468	24.8014257036619
52-53	24.47020425518243	24.99071163409273	25.72108310378736	24.81800100693748
54-55	24.0786668184038	24.85835304378197	26.005813856129272	25.05716628168495
56-57	24.22651949747985	24.970469235897244	25.825827775422	24.977183491200908
58-59	24.370434734781394	24.858597734318323	25.842114909528686	24.928852621371593
60-61	24.450535042777968	24.796633179194956	25.946666379675122	24.80616539835195
62-63	24.28055025381994	24.758859526203917	25.99793677878862	24.96265344118752
64-65	24.162892139358224	24.62928190294268	26.053926049449583	25.15389990824951
66-67	24.17991548282929	24.731833661677026	25.91443490933749	25.173815946156193
68-69	24.0624008694195	24.68851751642162	25.938495950048697	25.310585664110192
70-71	24.054493009053136	24.48658766307513	26.02613300816507	25.43278631970666
72-73	23.979395293846046	24.66089695734463	25.930428807656398	25.429278941152926
74-75	23.813329540772955	24.68970464980764	26.1666178084271	25.330348000992302
76-77	23.898316891762917	24.363818960945565	26.117459366743716	25.620404780547805
78-79	23.84708863232115	24.40987797750311	26.261601767459062	25.481431622716677
80-81	23.952396789938472	24.514195118253383	26.12661863821656	25.406789453591582
82-83	23.921371563297075	24.382222458993834	26.19914290953833	25.497263068170756
84-85	23.876787022865834	24.428166341924157	26.120925096142223	25.57412153906779
86-87	23.75198387766475	24.342383821938615	26.350368502715284	25.555263797681356
88-89	23.681536221860767	24.163799912054884	26.400116121705793	25.75454774437856
90-91	23.892941031240873	24.00182214082082	26.445594361607878	25.659642466330435
92-93	23.96523419067007	23.960249013037263	26.396686290966382	25.677830505326288
94-95	23.952673185062952	23.858539314849537	26.443011785260033	25.745775714827474
96-97	23.82618061512739	23.63957089154678	26.598826487960263	25.93542200536556
98	23.87904807404339	23.561461676029776	26.38839040725162	26.17109984267521
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	539.0
1	424.5
2	377.5
3	463.5
4	437.5
5	482.0
6	704.5
7	1240.5
8	1724.0
9	1752.5
10	1593.5
11	1468.5
12	1528.5
13	1646.0
14	1701.5
15	1800.5
16	1886.5
17	1925.5
18	1969.5
19	2016.5
20	2060.5
21	2067.5
22	2199.5
23	2415.0
24	2627.5
25	3081.0
26	3791.0
27	4717.0
28	6216.5
29	8655.5
30	11804.5
31	15957.5
32	20948.0
33	25727.5
34	30598.0
35	36415.0
36	43159.5
37	49813.0
38	56229.0
39	62483.5
40	68697.5
41	75461.5
42	83207.5
43	91162.0
44	98587.5
45	107458.0
46	117144.0
47	125377.0
48	131818.5
49	137022.0
50	139503.0
51	139919.5
52	140724.5
53	141957.5
54	141079.0
55	136331.0
56	128125.5
57	117651.0
58	105634.5
59	92302.5
60	79260.0
61	65669.5
62	52457.0
63	40169.5
64	29114.0
65	20853.5
66	14974.0
67	10877.5
68	7895.0
69	5656.0
70	4028.0
71	2876.0
72	2102.5
73	1542.0
74	1119.5
75	737.5
76	455.0
77	279.0
78	150.5
79	83.0
80	56.5
81	41.5
82	27.5
83	15.0
84	9.0
85	7.5
86	9.5
87	8.5
88	8.0
89	7.0
90	6.5
91	3.5
92	5.0
93	7.0
94	6.0
95	8.0
96	6.5
97	4.0
98	3.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28645567292564816
2	0.14057168542999
3	0.13822201336248288
4	0.13263728554985732
5	0.12831252681690947
6	0.047946931464492706
7	0.03633478400043588
8	0.013689393784606582
9	0.009092209304701386
10-11	0.0018729270103317465
12-13	0.00587418016876775
14-15	0.011186482234435977
16-17	0.006282818789203768
18-19	0.050398763187108814
20-21	0.09861812039855887
22-23	0.09860109378937404
24-25	0.07455952162038834
26-27	0.0848435935680281
28-29	0.1043390610846631
30-31	0.07873104087067269
32-33	0.019648706999298503
34-35	0.30477630440852965
36-37	0.39711160601788476
38-39	0.368047184139373
40-41	0.36367134557887065
42-43	0.3394595073180366
44-45	0.32210939255869075
46-47	0.3274046680151741
48-49	0.31996403980140165
50-51	0.3222796586505391
52-53	0.3703968562068801
54-55	0.3564350366753162
56-57	0.3395446403639608
58-59	0.3194702681350414
60-61	0.3291073289336575
62-63	0.32703008261310773
64-65	0.3461509647276764
66-67	0.367229906898501
68-69	0.325872273188539
70-71	0.3018817808471079
72-73	0.28652377936238754
74-75	0.27377084908294685
76-77	0.2768186121270321
78-79	0.2829311648243875
80-81	0.2737197692553923
82-83	0.28267576568661507
84-85	0.27603538810452977
86-87	0.2714722568429942
88-89	0.293453609300615
90-91	0.277755075632198
92-93	0.2689523186836388
94-95	0.28276089873253923
96-97	0.2818244352273733
98	0.2807006790211743
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2936580.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.158280481908456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.07154429249617	48.97037964460037
2	10.882847088821682	13.311524293997545
3	3.663367048347771	6.721356883531023
4	1.6487659668397998	4.033427657960539
5	0.9267520225649811	2.8339280066602535
6	0.5828889320872009	2.1389090879033477
7	0.3904177403492382	1.6714094368574133
8	0.28724808837839744	1.4054079325550446
9	0.2247463416289737	1.2370589818764832
>10	1.2488922866715795	13.759167049853316
>50	0.05959078656500667	2.416846707814422
>100	0.012547024917439984	1.139801955908865
>500	2.242173324121191E-4	0.0879672688248174
>1k	1.6816299927717486E-4	0.27281509165655865
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3376	0.11496366521599956	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.405321836966812E-5
2	0.0	0.0	0.0	0.0	3.405321836966812E-5
3	0.0	0.0	0.0	0.0	3.405321836966812E-5
4	0.0	0.0	0.0	0.0	3.405321836966812E-5
5	0.0	0.0	0.0	0.0	3.405321836966812E-5
6	0.0	0.0	0.0	0.0	3.405321836966812E-5
7	0.0	0.0	0.0	0.0	6.810643673933624E-5
8	3.405321836966812E-5	0.0	0.0	0.0	6.810643673933624E-5
9	3.405321836966812E-5	0.0	0.0	0.0	6.810643673933624E-5
10-11	3.405321836966812E-5	0.0	0.0	1.702660918483406E-5	6.810643673933624E-5
12-13	3.405321836966812E-5	0.0	0.0	3.405321836966812E-5	6.810643673933624E-5
14-15	5.107982755450218E-5	0.0	0.0	3.405321836966812E-5	2.2134591940284277E-4
16-17	6.810643673933624E-5	0.0	0.0	6.810643673933624E-5	2.3837252858767682E-4
18-19	6.810643673933624E-5	0.0	0.0	1.0215965510900435E-4	3.0647896532701304E-4
20-21	6.810643673933624E-5	3.405321836966812E-5	0.0	1.1918626429383841E-4	3.405321836966812E-4
22-23	6.810643673933624E-5	3.405321836966812E-5	0.0	5.448514939146899E-4	3.916120112511833E-4
24-25	6.810643673933624E-5	3.405321836966812E-5	0.0	0.001753740746037908	4.086386204360174E-4
26-27	6.810643673933624E-5	3.405321836966812E-5	0.0	0.0033712686185971437	4.4269183880568555E-4
28-29	6.810643673933624E-5	3.405321836966812E-5	0.0	0.009926513154758256	4.4269183880568555E-4
30-31	6.810643673933624E-5	3.405321836966812E-5	0.0	0.03161841325623685	4.4269183880568555E-4
32-33	6.810643673933624E-5	3.405321836966812E-5	0.0	0.07895238679007553	4.4269183880568555E-4
34-35	6.810643673933624E-5	3.405321836966812E-5	0.0	0.14879553766626485	4.4269183880568555E-4
36-37	6.810643673933624E-5	3.405321836966812E-5	0.0	0.2555694038643592	4.7674505717535363E-4
38-39	6.810643673933624E-5	3.405321836966812E-5	0.0	0.4200294219806714	5.107982755450217E-4
40-41	6.810643673933624E-5	3.405321836966812E-5	0.0	0.6069645642209645	5.107982755450217E-4
42-43	6.810643673933624E-5	3.405321836966812E-5	0.0	0.7877190473271629	5.107982755450217E-4
44-45	6.810643673933624E-5	3.405321836966812E-5	0.0	1.0008751677121004	5.107982755450217E-4
46-47	8.51330459241703E-5	3.405321836966812E-5	0.0	1.2493785287647534	5.278248847298559E-4
48-49	1.0215965510900435E-4	3.405321836966812E-5	0.0	1.5144147273358803	5.78904712284358E-4
50-51	1.0215965510900435E-4	3.405321836966812E-5	0.0	1.8144406077818416	5.78904712284358E-4
52-53	1.0215965510900435E-4	3.405321836966812E-5	0.0	2.1054423853598405	5.95931321469192E-4
54-55	1.0215965510900435E-4	3.405321836966812E-5	0.0	2.4137431978696307	6.129579306540261E-4
56-57	1.0215965510900435E-4	3.405321836966812E-5	0.0	2.769360957304075	6.129579306540261E-4
58-59	1.0215965510900435E-4	3.405321836966812E-5	0.0	3.16492314188614	6.129579306540261E-4
60-61	1.0215965510900435E-4	3.405321836966812E-5	0.0	3.608279018450034	6.299845398388601E-4
62-63	1.0215965510900435E-4	3.405321836966812E-5	0.0	4.06474538408625	7.151175857630305E-4
64-65	1.0215965510900435E-4	3.405321836966812E-5	0.0	4.528618324717868	7.321441949478645E-4
66-67	1.0215965510900435E-4	6.810643673933624E-5	0.0	5.036794502448426	7.661974133175327E-4
68-69	1.0215965510900435E-4	6.810643673933624E-5	0.0	5.604938397727969	7.832240225023667E-4
70-71	1.0215965510900435E-4	6.810643673933624E-5	0.0	6.243947040434792	7.832240225023667E-4
72-73	1.0215965510900435E-4	6.810643673933624E-5	0.0	6.913416286973282	7.832240225023667E-4
74-75	1.0215965510900435E-4	6.810643673933624E-5	0.0	7.583804970407753	7.832240225023667E-4
76-77	1.0215965510900435E-4	6.810643673933624E-5	0.0	8.283138889456442	8.343038500568689E-4
78-79	1.0215965510900435E-4	6.810643673933624E-5	0.0	9.038881964734488	8.513304592417029E-4
80-81	1.0215965510900435E-4	6.810643673933624E-5	0.0	9.873645533239346	8.683570684265369E-4
82-83	1.0215965510900435E-4	6.810643673933624E-5	0.0	10.748489739765304	8.853836776113711E-4
84-85	1.0215965510900435E-4	6.810643673933624E-5	0.0	11.622142083648326	8.853836776113711E-4
86	1.0215965510900435E-4	6.810643673933624E-5	0.0	12.270804813762949	8.853836776113711E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3900	0.0	43.189964	1
GTATCAA	6595	0.0	38.79952	1
TATCAAC	8485	0.0	29.731682	2
TCAACGC	8520	0.0	29.446241	4
CAACGCA	8760	0.0	28.690477	5
ATCAACG	8920	0.0	28.126259	3
GTACATG	11165	0.0	27.61738	1
AACGCAG	9290	0.0	27.22039	6
TACATGG	11525	0.0	26.314917	2
ACATGGG	12325	0.0	23.860374	3
CATGGGG	8965	0.0	22.910471	4
ACGCAGA	10685	0.0	22.716606	7
CGCAGAG	11075	0.0	21.747227	8
GTGGTAT	2000	0.0	19.7895	1
GTATATA	685	0.0	18.81195	1
ATGGGGG	5285	0.0	18.778605	5
GCAGAGT	11875	0.0	18.696619	9
GAGTACA	8835	0.0	17.971275	1
AGAGTAC	9920	0.0	17.526438	10-11
AGTACAT	8350	0.0	17.445032	2
>>END_MODULE