FastQCFastQC Report
Mon 15 Aug 2016
SRR2921573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2936580
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.048.264821
GTATCAA61700.043.262631
TCAACGC79850.032.414094
TATCAAC80850.032.304112
CAACGCA82650.031.4303535
ATCAACG85550.030.4209483
AACGCAG86750.030.1050076
GTACATG105100.026.9822881
TACATGG110650.024.8915392
ACGCAGA100750.024.8376837
CGCAGAG105150.023.4822448
ACATGGG117150.022.9854473
GTATAAT6950.021.223261
CATGGGG88500.021.0356034
GCAGAGT113250.019.9977559
GAGTACA83900.019.6789881
AGTACAT81800.018.9832082
AGAGTAC91900.018.52049310-11
GTATAGG4950.017.3022461
GTGGTAT17600.017.03191
Produced by FastQC (version 0.11.5)