##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921573_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2936580
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.95146565051863	31.0	30.0	34.0	26.0	34.0
2	29.939747938077627	31.0	30.0	34.0	26.0	34.0
3	30.11528785185488	31.0	30.0	34.0	26.0	34.0
4	33.773606712570405	35.0	35.0	37.0	30.0	37.0
5	33.27239407746426	35.0	33.0	37.0	27.0	37.0
6	33.16299572972642	35.0	33.0	37.0	27.0	37.0
7	32.96950023496721	35.0	33.0	37.0	26.0	37.0
8	32.87385768478979	35.0	33.0	37.0	26.0	37.0
9	34.17598328668043	37.0	34.0	39.0	26.0	39.0
10-11	33.95762741011653	37.0	33.0	39.0	25.0	39.0
12-13	33.779014874445785	37.0	33.0	39.0	25.0	39.0
14-15	34.625667272813956	38.0	33.0	40.0	25.0	41.0
16-17	34.479810698159085	38.0	33.0	40.0	24.0	41.0
18-19	34.357270532388014	38.0	33.0	40.0	23.0	41.0
20-21	34.22001563042723	38.0	33.0	40.0	22.0	41.0
22-23	34.04591804071403	38.0	33.0	40.0	20.5	41.0
24-25	33.88836078022734	38.0	33.0	40.0	19.0	41.0
26-27	33.79091698506426	38.0	32.5	40.0	18.5	41.0
28-29	33.78380582173821	38.0	32.5	40.0	18.5	41.0
30-31	33.61040836619469	38.0	32.0	40.0	18.0	41.0
32-33	33.42679239114889	38.0	32.0	40.0	17.5	41.0
34-35	33.2123987768084	38.0	32.0	40.0	16.0	41.0
36-37	33.02983402461366	38.0	31.5	40.0	15.5	41.0
38-39	32.84546138705569	37.5	31.0	40.0	15.0	41.0
40-41	32.84196820791533	38.0	31.5	40.0	14.0	41.0
42-43	32.62285107165478	37.0	31.0	40.0	9.0	41.0
44-45	32.40119254370731	37.0	31.0	40.0	8.5	41.0
46-47	32.116477330772526	37.0	30.5	40.0	7.5	41.0
48-49	31.808845323471523	37.0	30.0	40.0	4.5	41.0
50-51	31.53442933616656	36.0	30.0	40.0	2.0	41.0
52-53	31.201113880772873	36.0	29.5	39.0	2.0	41.0
54-55	30.879091664453206	35.5	29.0	39.0	2.0	40.5
56-57	30.534743136573837	35.0	28.0	39.0	2.0	40.0
58-59	30.05806397237603	35.0	28.0	39.0	2.0	40.0
60-61	29.696686111054355	34.5	28.0	38.0	2.0	40.0
62-63	29.3611997629896	34.0	27.0	38.0	2.0	40.0
64-65	28.909233870693118	34.0	26.0	37.5	2.0	40.0
66-67	28.493097072104284	34.0	26.0	37.0	2.0	39.0
68-69	28.065937927793556	33.5	26.0	36.0	2.0	39.0
70-71	27.61024610260916	33.0	25.5	36.0	2.0	39.0
72-73	27.148668519161745	33.0	24.5	35.0	2.0	38.0
74-75	26.630645342541325	32.0	23.5	35.0	2.0	37.0
76-77	25.15112835339068	30.5	21.0	34.0	2.0	35.5
78-79	26.028703457763793	32.0	23.0	35.0	2.0	36.0
80-81	26.090470717637523	32.0	23.0	35.0	2.0	36.0
82-83	25.78726869351422	32.0	21.5	35.0	2.0	35.5
84-85	25.380949437781364	32.0	20.0	35.0	2.0	35.0
86-87	24.953922419957912	32.0	18.5	34.0	2.0	35.0
88-89	24.52510386231603	31.0	16.5	34.0	2.0	35.0
90-91	24.114439756451382	31.0	9.0	34.0	2.0	35.0
92-93	23.712828868956404	31.0	4.5	34.0	2.0	35.0
94-95	23.340714368414957	31.0	2.0	34.0	2.0	35.0
96-97	22.8362123626804	31.0	2.0	34.0	2.0	35.0
98-99	22.10724873832826	30.5	2.0	34.0	2.0	35.0
100-101	19.583769895592834	26.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84441.0
3	36980.0
4	19105.0
5	13755.0
6	11957.0
7	11929.0
8	13568.0
9	15503.0
10	17333.0
11	19643.0
12	21574.0
13	23919.0
14	25710.0
15	27419.0
16	29099.0
17	29868.0
18	29063.0
19	27784.0
20	27933.0
21	28932.0
22	31080.0
23	34337.0
24	40317.0
25	47036.0
26	55267.0
27	65196.0
28	76864.0
29	90967.0
30	105470.0
31	124670.0
32	148751.0
33	179829.0
34	223560.0
35	283039.0
36	360567.0
37	376123.0
38	169310.0
39	8682.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.16193267825797	18.79955260370785	12.997906712343777	25.040608005690412
2	16.412765870502422	21.182055316047922	37.25943103882748	25.14574777462218
3	19.381668260925384	25.11793484867936	28.98273807719799	26.517658813197258
4	14.496570054199342	16.78755996700495	35.015724416405156	33.700145562390546
5	14.886352451297435	37.0537975211664	33.301148208582774	14.758701818953393
6	33.13792318223221	34.577029120282745	17.48631663057766	14.798731066907381
7	28.968982909075162	31.406232470813535	21.198632344100425	18.426152276010882
8	27.17559980382273	33.45985494068639	20.21884406840011	19.145701187090772
9	27.631205741457155	15.08987049539568	19.12245187384176	38.15647188930541
10-11	26.24705092188523	24.753180913157323	27.274080160375302	21.725688004582153
12-13	26.892334677313524	22.29456241906891	27.21713273091117	23.595970172706394
14-15	24.36643588093147	23.56825452889163	24.589505272115336	27.475804318061563
16-17	24.308513683533278	26.333168170399073	25.23982135140993	24.118496794657716
18-19	24.35654884680692	25.80300083238125	26.479334026842377	23.361116293969452
20-21	25.13619236505039	25.032512743598556	26.158695578846086	23.67259931250496
22-23	24.98694732307659	24.790185886477836	25.973958035132487	24.248908755313085
24-25	24.62794548482526	24.980038842044962	25.996055818315465	24.39595985481431
26-27	24.389859985291736	25.168696165306315	26.06838065479887	24.373063194603088
28-29	24.47153905273055	25.356588844976898	25.89142315209604	24.280448950196515
30-31	24.85235359650884	25.182689803656473	25.66553080063652	24.299425799198165
32-33	24.614080913257098	25.31202014016698	25.584024814102886	24.489874132473037
34-35	24.785717522223745	25.270904359101287	25.79216975935019	24.151208359324773
36-37	24.859522189788034	25.1067188505466	25.76154387796085	24.272215081704516
38-39	24.78254574689698	25.151876461660837	25.68167688374335	24.383900907698834
40-41	24.773781875880573	25.025268751372003	25.713294062528895	24.48765531021852
42-43	24.518742822125304	25.037908716254087	25.669263631596255	24.77408483002435
44-45	24.35352421200772	25.018668767445824	25.838510776455536	24.78929624409092
46-47	24.557561500437462	24.973017421284702	25.673942808344407	24.795478269933426
48-49	24.411690038900847	25.10574842711825	25.60526001692111	24.877301517059795
50-51	24.357976176609426	25.1957013813534	25.687475179710816	24.758847262326363
52-53	24.462635612873214	25.085893332375225	25.61403281732344	24.837438237428124
54-55	24.037946729625123	24.972003830911312	25.805979736495427	25.184069702968138
56-57	24.197691771691918	25.025678767473654	25.653412245025848	25.123217215808584
58-59	24.30299901306581	24.968123864668097	25.705021104317378	25.023856017948713
60-61	24.414822808387257	24.991313802674124	25.64323845962042	24.9506249293182
62-63	24.2188073392152	24.93396651010941	25.67228487028896	25.17494128038643
64-65	24.05146820165094	24.789766710953792	25.752747737422872	25.406017349972398
66-67	24.101521732241775	24.87109349312186	25.59827849802326	25.429106276613105
68-69	23.994361917588712	24.852990340898828	25.52992487895878	25.622722862553683
70-71	23.936043014063916	24.680967898193146	25.588670519601113	25.794318568141822
72-73	23.87207343188079	24.876446567505916	25.408003161877566	25.843476838735725
74-75	23.584828201330808	24.9134048035421	25.593833352241695	25.907933642885396
76-77	23.572464759725946	24.575251767431084	25.749995819659066	26.102287653183904
78-79	23.519769240154233	24.588454646516077	25.810191575111794	26.081584538217896
80-81	23.563448187544957	24.72857109771781	25.64435201735074	26.063628697386488
82-83	23.455060604239105	24.601808157158086	25.69229451509542	26.250836723507387
84-85	23.383237092073134	24.626045395355554	25.538932323200324	26.451785189370984
86-87	23.101782735895735	24.637344243209228	25.8096317166425	26.451241304252537
88-89	23.028117718356587	24.44109621536557	25.824713143625928	26.70607292265192
90-91	23.077636212305524	24.32901807627462	25.815173826749472	26.778171884670382
92-93	23.088301981631528	24.26822505477214	25.738194622768823	26.90527834082751
94-95	22.922471444070837	24.21349026295769	25.82315177363122	27.04088651934025
96-97	22.674554330907633	24.034468061992246	26.006917655448447	27.284059951651674
98-99	22.4841772960161	24.07113022719649	25.949421834905813	27.495270641881596
100-101	22.747763087902456	23.93332479684404	25.99435910603886	27.324553009214643
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	509.0
1	378.5
2	327.0
3	408.0
4	370.0
5	396.5
6	573.0
7	1001.0
8	1343.0
9	1342.5
10	1142.5
11	925.0
12	868.0
13	874.0
14	899.0
15	984.0
16	1118.0
17	1180.5
18	1236.5
19	1346.0
20	1458.5
21	1586.5
22	1749.0
23	1933.5
24	2236.5
25	2648.0
26	3164.5
27	3978.0
28	5176.0
29	6864.0
30	9466.0
31	13086.0
32	17574.5
33	22102.0
34	26638.5
35	31553.0
36	37376.0
37	43515.0
38	49556.0
39	55858.0
40	61904.0
41	68355.0
42	75146.5
43	82557.0
44	90446.5
45	98017.5
46	106370.5
47	115532.5
48	123793.0
49	130424.5
50	135839.5
51	138952.0
52	140194.5
53	141788.5
54	141285.5
55	137038.0
56	129390.0
57	118141.5
58	106336.5
59	93941.0
60	80731.0
61	67133.0
62	53546.0
63	41109.0
64	29745.5
65	20979.5
66	15051.5
67	10994.5
68	7899.0
69	5718.0
70	4195.0
71	2955.5
72	2074.0
73	1547.5
74	1125.5
75	765.0
76	482.0
77	265.0
78	150.5
79	83.0
80	38.0
81	21.0
82	12.5
83	7.5
84	4.0
85	2.5
86	0.5
87	0.0
88	0.0
89	0.0
90	1.0
91	1.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22948463859319346
2	0.0
3	3.405321836966812E-5
4	0.013008329417213221
5	0.01501746930102364
6	0.10529255119901382
7	0.1352253301459521
8	0.15453350496155394
9	0.11060485326468204
10-11	0.11782413555905169
12-13	0.09388472304517499
14-15	0.1161895810773076
16-17	0.07164797144978172
18-19	0.07585354391843574
20-21	0.05370192536896662
22-23	0.07886725374415136
24-25	0.07256740834576275
26-27	0.050926588071838676
28-29	0.01806523234510894
30-31	0.031822732566454855
32-33	0.03924633417104251
34-35	0.044916195029592244
36-37	0.03619857112695721
38-39	0.038650402849573315
40-41	0.021845139584142095
42-43	0.03097140210721315
44-45	0.017929019471630264
46-47	0.03029033773981979
48-49	3.9161201125118337E-4
50-51	0.016141225507222686
52-53	0.007985479707687173
54-55	0.015800693323526006
56-57	0.010284071947639771
58-59	0.007287388731108978
60-61	0.03023925791226529
62-63	0.01295724958965872
64-65	0.014881256427544967
66-67	0.057924524446805466
68-69	0.0765856881133836
70-71	0.033440260439014095
72-73	0.05499594766701402
74-75	0.04668696238481499
76-77	0.21102779423683332
78-79	0.0774370185726253
80-81	0.01539205470308999
82-83	0.009381661660843567
84-85	0.024824796191488058
86-87	0.17184956650253017
88-89	0.37414271022754364
90-91	0.27302167827881413
92-93	0.27528621730039704
94-95	0.0021112995389194235
96-97	0.006146605915725095
98-99	0.004597184479905196
100-101	0.005652834249364907
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2936580.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.143875666882295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.92513503778378	45.30869362961913
2	12.05035812348874	14.013090489470686
3	3.9896440577445618	6.959201041458467
4	1.7949023927039853	4.174503262222802
5	1.0402874043308792	3.024317074761917
6	0.6370530252713735	2.2224439136753977
7	0.45574505067399246	1.854914849352991
8	0.33168192355468057	1.5428218019292566
9	0.25249351075571047	1.3212856166467173
>10	1.435350871597372	15.104059896409122
>50	0.07035185718553674	2.7291069062804265
>100	0.016525112623948467	1.3871147864340936
>500	2.947701784851233E-4	0.11411199868052362
>1k	1.7686210702510147E-4	0.24433473305855583
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.405321836966812E-5
2	0.0	0.0	0.0	0.0	3.405321836966812E-5
3	0.0	0.0	0.0	0.0	3.405321836966812E-5
4	0.0	0.0	0.0	0.0	6.810643673933624E-5
5	0.0	0.0	0.0	0.0	6.810643673933624E-5
6	0.0	0.0	0.0	0.0	6.810643673933624E-5
7	0.0	0.0	0.0	0.0	6.810643673933624E-5
8	0.0	0.0	0.0	0.0	6.810643673933624E-5
9	0.0	0.0	0.0	3.405321836966812E-5	1.0215965510900435E-4
10-11	0.0	0.0	0.0	3.405321836966812E-5	1.0215965510900435E-4
12-13	0.0	0.0	0.0	3.405321836966812E-5	1.3621287347867248E-4
14-15	0.0	0.0	0.0	3.405321836966812E-5	1.702660918483406E-4
16-17	0.0	0.0	0.0	3.405321836966812E-5	2.043193102180087E-4
18-19	0.0	0.0	0.0	3.405321836966812E-5	2.043193102180087E-4
20-21	0.0	3.405321836966812E-5	0.0	5.107982755450218E-5	2.5539913777251086E-4
22-23	0.0	3.405321836966812E-5	0.0	3.7458540206634927E-4	2.7242574695734496E-4
24-25	0.0	3.405321836966812E-5	0.0	0.0016345544817440696	2.7242574695734496E-4
26-27	0.0	3.405321836966812E-5	0.0	0.0032691089634881393	2.7242574695734496E-4
28-29	0.0	3.405321836966812E-5	0.0	0.01023299212008527	2.7242574695734496E-4
30-31	0.0	3.405321836966812E-5	0.0	0.0320951583134122	2.89452356142179E-4
32-33	0.0	3.405321836966812E-5	0.0	0.07917373270947836	3.405321836966812E-4
34-35	0.0	3.405321836966812E-5	0.0	0.14959578829795206	3.405321836966812E-4
36-37	0.0	6.810643673933624E-5	0.0	0.25630154805930705	3.575587928815152E-4
38-39	0.0	1.0215965510900435E-4	0.0	0.4218342425542638	3.7458540206634927E-4
40-41	0.0	1.0215965510900435E-4	0.0	0.6110509504253248	3.7458540206634927E-4
42-43	0.0	1.0215965510900435E-4	0.0	0.7952618351960443	3.7458540206634927E-4
44-45	0.0	1.0215965510900435E-4	0.0	1.0117211177628398	3.7458540206634927E-4
46-47	0.0	1.0215965510900435E-4	0.0	1.2616717405962037	3.7458540206634927E-4
48-49	0.0	1.0215965510900435E-4	0.0	1.5261460610642312	4.7674505717535363E-4
50-51	0.0	1.0215965510900435E-4	0.0	1.8245714402468178	4.7674505717535363E-4
52-53	0.0	1.0215965510900435E-4	0.0	2.1129000401827978	5.278248847298559E-4
54-55	0.0	1.0215965510900435E-4	0.0	2.419668457865953	5.78904712284358E-4
56-57	0.0	1.0215965510900435E-4	0.0	2.7728684387961504	5.78904712284358E-4
58-59	0.0	1.0215965510900435E-4	0.0	3.1631523745309167	5.78904712284358E-4
60-61	0.0	1.0215965510900435E-4	0.0	3.601791880350612	5.78904712284358E-4
62-63	0.0	1.0215965510900435E-4	0.0	4.052315959381321	6.129579306540261E-4
64-65	0.0	1.0215965510900435E-4	0.0	4.510791464901348	6.129579306540261E-4
66-67	0.0	1.0215965510900435E-4	0.0	5.012872116543734	6.129579306540261E-4
68-69	0.0	1.0215965510900435E-4	0.0	5.573439170736027	6.129579306540261E-4
70-71	0.0	1.0215965510900435E-4	0.0	6.204905025573967	6.470111490236943E-4
72-73	0.0	1.0215965510900435E-4	0.0	6.867308229300751	6.980909765781964E-4
74-75	0.0	1.0215965510900435E-4	0.0	7.537747992562777	7.151175857630305E-4
76-77	0.0	1.0215965510900435E-4	0.0	8.2391761845412	7.151175857630305E-4
78-79	0.0	1.0215965510900435E-4	0.0	8.995804643496857	7.151175857630305E-4
80-81	0.0	1.0215965510900435E-4	0.0	9.82857609872709	7.151175857630305E-4
82-83	0.0	1.0215965510900435E-4	0.0	10.703624624563268	7.491708041326985E-4
84-85	0.0	1.0215965510900435E-4	0.0	11.581533620742498	7.661974133175327E-4
86-87	0.0	1.0215965510900435E-4	0.0	12.459153164565583	7.832240225023667E-4
88-89	0.0	1.0215965510900435E-4	0.0	13.381739983245815	7.832240225023667E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3480	0.0	48.26482	1
GTATCAA	6170	0.0	43.26263	1
TCAACGC	7985	0.0	32.41409	4
TATCAAC	8085	0.0	32.30411	2
CAACGCA	8265	0.0	31.430353	5
ATCAACG	8555	0.0	30.420948	3
AACGCAG	8675	0.0	30.105007	6
GTACATG	10510	0.0	26.982288	1
TACATGG	11065	0.0	24.891539	2
ACGCAGA	10075	0.0	24.837683	7
CGCAGAG	10515	0.0	23.482244	8
ACATGGG	11715	0.0	22.985447	3
GTATAAT	695	0.0	21.22326	1
CATGGGG	8850	0.0	21.035603	4
GCAGAGT	11325	0.0	19.997755	9
GAGTACA	8390	0.0	19.678988	1
AGTACAT	8180	0.0	18.983208	2
AGAGTAC	9190	0.0	18.520493	10-11
GTATAGG	495	0.0	17.302246	1
GTGGTAT	1760	0.0	17.0319	1
>>END_MODULE