FastQCFastQC Report
Mon 15 Aug 2016
SRR2921572_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921572_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5911472
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC92380.1562724140450974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA162800.040.2775761
GGTATCA97150.036.660731
TATCAAC202950.031.8105812
TCAACGC211450.030.3577354
ATATGTA44050.030.2938861
ATCAACG214100.029.9822393
CAACGCA215950.029.5746865
AACGCAG226500.028.411696
GTACATG374800.028.3851471
TATGTAA47800.028.2620562
TACATGG384550.027.2795622
GTAAGTA86400.026.2190822
TGTAAGT85200.026.032171
ACATGGG405500.025.4962313
ATTATAT30650.025.0719951
CATGGGG351400.024.3346584
ATGGGGG275550.024.2116725
TGGGGGG254550.023.585876
ACGCAGA269300.023.5338927
CGCAGAG276550.022.7330448
Produced by FastQC (version 0.11.5)