##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921572_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5911472
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.12018884636517	31.0	28.0	31.0	16.0	34.0
2	28.08609310845082	31.0	28.0	33.0	16.0	34.0
3	28.17088823223725	31.0	28.0	33.0	16.0	34.0
4	31.594616027953784	35.0	32.0	37.0	18.0	37.0
5	31.526872325539223	35.0	32.0	37.0	17.0	37.0
6	31.62375394825519	35.0	32.0	37.0	18.0	37.0
7	31.433166561560302	35.0	32.0	37.0	17.0	37.0
8	31.384765249670473	35.0	32.0	37.0	17.0	37.0
9	32.582656739302834	37.0	32.0	39.0	17.0	39.0
10-11	32.33717380374973	37.0	32.0	39.0	16.0	39.0
12-13	32.08821499958047	37.0	32.0	39.0	12.0	39.0
14-15	32.741712639423824	38.0	31.5	40.0	10.5	41.0
16-17	32.574290718115556	37.0	31.0	40.0	10.0	41.0
18-19	32.402019581586444	37.0	31.0	40.0	10.0	41.0
20-21	32.27359759125984	37.0	31.0	40.0	10.0	41.0
22-23	32.082523862077	37.0	30.5	40.0	9.0	41.0
24-25	32.16730088546474	37.0	31.0	40.0	8.0	41.0
26-27	31.913255953847028	37.0	30.0	40.0	7.5	41.0
28-29	31.709906855686704	37.0	30.0	40.0	2.0	41.0
30-31	31.438268928618797	37.0	30.0	40.0	2.0	41.0
32-33	31.184797711974277	36.0	30.0	40.0	2.0	41.0
34-35	30.859618636441144	36.0	29.5	39.5	2.0	41.0
36-37	30.57428564323742	36.0	29.0	39.0	2.0	41.0
38-39	30.363175956851357	35.5	28.0	39.0	2.0	40.0
40-41	30.143269899612143	35.0	27.5	39.0	2.0	40.0
42-43	29.909987901490524	35.0	27.0	39.0	2.0	40.0
44-45	29.66965469852517	35.0	26.5	39.0	2.0	40.0
46-47	29.357609915093906	35.0	26.0	39.0	2.0	40.0
48-49	29.07013929863831	35.0	25.0	39.0	2.0	40.0
50-51	28.172799938830803	33.5	23.5	38.0	2.0	39.5
52-53	28.396330051127705	33.5	25.0	38.0	2.0	39.5
54-55	28.98653093510381	35.0	26.0	39.0	2.0	40.0
56-57	28.986643343654507	35.0	26.0	39.0	2.0	40.0
58-59	28.739888305315496	34.5	26.0	39.0	2.0	40.0
60-61	28.37219832894413	34.0	25.0	38.0	2.0	40.0
62-63	27.904515237490763	34.0	23.5	38.0	2.0	40.0
64-65	27.459845449661266	34.0	22.5	37.5	2.0	40.0
66-67	27.12268763177767	33.0	22.0	37.0	2.0	39.0
68-69	26.661906797494773	33.0	21.5	36.0	2.0	39.0
70-71	26.222825550049123	32.5	20.0	36.0	2.0	39.0
72-73	25.72559042823852	32.0	18.5	35.0	2.0	38.0
74-75	25.269806234386294	32.0	16.5	35.0	2.0	37.0
76-77	24.839394401259113	31.5	12.0	35.0	2.0	36.5
78-79	24.43361112088495	31.0	7.5	35.0	2.0	36.0
80-81	23.99796040647744	31.0	4.5	34.0	2.0	36.0
82-83	23.44356363355861	31.0	2.0	34.0	2.0	35.0
84-85	23.03486382072012	30.0	2.0	34.0	2.0	35.0
86-87	22.54521022851838	30.0	2.0	34.0	2.0	35.0
88-89	22.047588231831263	30.0	2.0	34.0	2.0	35.0
90-91	21.4349787159611	29.0	2.0	34.0	2.0	35.0
92-93	20.858057519345437	29.0	2.0	34.0	2.0	35.0
94-95	20.14902557264925	28.5	2.0	34.0	2.0	35.0
96-97	19.50666872819494	27.0	2.0	33.5	2.0	35.0
98	19.050122372228103	26.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	364544.0
3	64606.0
4	49421.0
5	38382.0
6	34195.0
7	35963.0
8	40309.0
9	44697.0
10	47159.0
11	49172.0
12	49696.0
13	48893.0
14	48454.0
15	51100.0
16	55022.0
17	59580.0
18	61849.0
19	62537.0
20	64971.0
21	69066.0
22	75753.0
23	83526.0
24	94875.0
25	107578.0
26	123163.0
27	141669.0
28	161325.0
29	186036.0
30	213845.0
31	249010.0
32	293977.0
33	355442.0
34	436097.0
35	506818.0
36	614188.0
37	626246.0
38	285427.0
39	16881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.337328140355595	16.76378905546357	13.203878653665905	24.695004150514936
2	16.288919980080557	19.42663852650336	39.939818218158536	24.344623275257547
3	20.552830320028903	24.26461409315546	28.042388445686417	27.140167141129222
4	14.849815080414105	14.674244790308165	34.67514923305288	35.80079089622485
5	15.181949501161249	38.53812394221068	31.43773987354882	14.84218668307925
6	34.51427673978859	31.883407259705727	17.975235948644116	15.627080051861569
7	30.21185959553108	32.63668987382577	19.05326771499648	18.098182815646666
8	29.020046856981992	33.95748967305683	19.183278202164463	17.83918526779672
9	30.774887784953137	15.51225416056784	17.554743957508173	36.158114096970856
10-11	27.823692601806165	24.873318251095323	25.49659186362684	21.80639728347167
12-13	27.782904621145825	21.42537166606921	26.315001489557115	24.476722223227853
14-15	25.607686118306898	22.92642835294468	24.012394803873004	27.45349072487542
16-17	25.443848294318073	25.833314372054467	24.61133960039549	24.111497733231975
18-19	25.538978950599162	26.269427828866792	25.66431964551228	22.527273575021763
20-21	25.83064005773875	24.8869501537654	25.96431118815255	23.3180986003433
22-23	25.983429228297407	23.945980835230152	25.54460838670756	24.52598154976489
24-25	25.426358833100743	24.198896018991938	25.211761690694367	25.162983457212952
26-27	24.72737990977308	24.778398564468635	25.73739182343736	24.75682970232092
28-29	24.628623513562754	26.42491227167098	24.536636609991785	24.40982760477448
30-31	26.76329125581156	24.937838485696755	24.714500561349624	23.58436969714206
32-33	25.938082363800312	24.805554096275433	24.767367484887146	24.48899605503711
34-35	25.663519690029347	25.470169744016175	25.321318956412455	23.544991609542024
36-37	25.33486748734794	25.695630827734274	25.652783070899666	23.31671861401812
38-39	25.899660429912757	25.52025924418008	24.92150269078464	23.658577635122523
40-41	25.929462507224592	25.029377891428684	24.616440925528764	24.42471867581796
42-43	25.007604774703935	25.261614856195724	24.399655538706966	25.33112483039337
44-45	24.41210136116728	25.157280298790997	25.5066327678516	24.923985572190123
46-47	24.4090522675635	24.474974250551046	25.23097298131956	25.885000500565898
48-49	24.354290844223435	25.201526818195276	25.603702397536892	24.8404799400444
50-51	24.862956740872306	24.658971962188666	25.954916683026287	24.52315461391274
52-53	24.822934796650227	24.837491124035356	25.53723882001912	24.802335259295297
54-55	23.144837236230174	24.281696657517376	26.71514831805722	25.858317788195233
56-57	24.221721729578356	24.35016164660364	25.5808242776135	25.847292346204505
58-59	24.650690508626248	25.035177398777915	25.809937019179298	24.504195073416543
60-61	26.032173665303134	24.41830651826907	25.94520153252856	23.604318283899236
62-63	25.292492872421697	24.287140358908903	26.10541519479898	24.31495157387042
64-65	24.300130169661813	23.897647951312813	26.50993413449055	25.292287744534825
66-67	24.737410270083686	24.37967459794946	25.751417630192353	25.131497501774504
68-69	23.769334699271006	24.644137179169025	26.109644071427514	25.47688405013246
70-71	24.123301066071605	24.176585985506367	26.04670683590722	25.653406112514816
72-73	23.77702311264905	25.382942892648607	25.416416869832876	25.42361712486947
74-75	23.59705512312196	24.70646755453467	26.128349803384587	25.568127518958782
76-77	23.372202840092353	23.69994037666581	26.059713392472737	26.868143390769095
78-79	23.20443263381202	24.096683793214428	26.644549292782553	26.054334280191
80-81	24.033035209511336	24.33334681619242	25.719876558065202	25.91374141623104
82-83	23.97038826874343	24.51154958607248	26.069597558549347	25.448464586634746
84-85	23.966011522440557	24.73579107747344	25.38878421283689	25.909413187249115
86-87	22.962995373796353	25.326983146583338	25.679538582039797	26.030482897580516
88-89	22.863598524380837	24.145295647568066	26.450486225759214	26.540619602291883
90-91	24.091422682776336	23.276839923976684	26.541285184781906	26.090452208465077
92-93	24.492964969506332	23.368772760866623	25.985672504008217	26.152589765618828
94-95	23.97774999160422	23.91617244630007	25.737599091423785	26.368478470671924
96-97	22.904358496734382	23.68243342086595	26.35936259068276	27.05384549171691
98	22.859148116563237	23.172194892607166	25.813494203792313	28.15516278703728
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	223.0
1	232.5
2	336.5
3	450.0
4	449.0
5	506.0
6	761.0
7	1440.5
8	2084.0
9	2257.0
10	2165.0
11	2119.0
12	2322.0
13	2685.5
14	3134.5
15	3524.5
16	3759.5
17	3814.0
18	3834.5
19	3834.0
20	3731.5
21	3643.0
22	3563.5
23	3525.5
24	3667.0
25	3975.0
26	4411.5
27	5128.5
28	6466.0
29	8786.0
30	12231.0
31	17278.0
32	24460.0
33	33208.5
34	43352.0
35	55283.0
36	68516.0
37	81508.5
38	95245.0
39	109797.0
40	124317.5
41	139224.5
42	156235.5
43	174834.5
44	194159.5
45	218449.5
46	249184.0
47	279043.0
48	301703.0
49	322322.0
50	333338.0
51	335224.0
52	337636.5
53	334197.0
54	323251.0
55	307882.5
56	286627.5
57	257331.0
58	217302.0
59	173224.0
60	137213.5
61	108508.0
62	84851.5
63	63759.5
64	45371.0
65	32353.0
66	23444.5
67	17044.0
68	12132.5
69	8497.5
70	5952.5
71	4143.5
72	2876.5
73	1926.5
74	1310.5
75	851.0
76	480.5
77	296.0
78	187.0
79	116.5
80	79.5
81	57.5
82	44.0
83	30.5
84	23.0
85	20.5
86	18.0
87	17.5
88	18.0
89	14.0
90	12.5
91	13.5
92	9.5
93	6.0
94	9.0
95	10.0
96	9.0
97	11.5
98	6.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.26758140781179374
2	0.130119875388059
3	0.1285466631661285
4	0.12321804112410582
5	0.11941188252266102
6	0.045081834101557104
7	0.032902126576933796
8	0.012179707524623308
9	0.008458130225432854
10-11	0.0017508329566646005
12-13	0.005396287083826161
14-15	0.01028508635412635
16-17	0.005117168786386877
18-19	0.04696799714182864
20-21	0.0940459499765879
22-23	0.09356383655373822
24-25	0.06976265809937017
26-27	0.08135029650821318
28-29	0.09709933498796916
30-31	0.07240159472970523
32-33	0.01731379257146105
34-35	0.2872127280650234
36-37	0.37241993195603396
38-39	0.34418669326353907
40-41	0.34064273669908274
42-43	0.3178988245228938
44-45	0.30202291408975634
46-47	0.30689479709960565
48-49	0.3007288201652651
50-51	0.3027164807682418
52-53	0.34784906365115154
54-55	0.33423993211843006
56-57	0.3185923912013793
58-59	0.3006526969932362
60-61	0.3079605215080102
62-63	0.30730078735042643
64-65	0.3241493827594887
66-67	0.3449310087233772
68-69	0.30592211212368087
70-71	0.2832796975101971
72-73	0.2680804374950943
74-75	0.25480117304116473
76-77	0.2586580804239621
78-79	0.2648155992280772
80-81	0.2553763258964941
82-83	0.2644518996283836
84-85	0.25777843488051705
86-87	0.25294038439156946
88-89	0.2760649124279029
90-91	0.2602735832970198
92-93	0.2510034725699454
94-95	0.2648071410978518
96-97	0.26346229839200797
98	0.26301401749006
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5911472.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.422983038374156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.449334542323	38.548034989225435
2	11.512263742504473	11.609653588431856
3	4.403345542185268	6.660894527571245
4	2.2730445823411016	4.584547536834145
5	1.2868919220218258	3.2444464778163615
6	0.8144900102925602	2.464140958434417
7	0.5947348384853518	2.099181327129396
8	0.4110096787241622	1.6579467247132835
9	0.3170912087385042	1.438981617585524
>10	1.7618997125297402	16.2775605329072
>50	0.11847993298711836	4.099491051750673
>100	0.05281591770060157	4.6010172748785125
>500	0.0030430513324997987	1.0453138734087388
>1k	0.001487695319361505	1.415003397141822
>5k	6.762251450578972E-5	0.253786122171219
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9238	0.1562724140450974	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6916260450865707E-5
2	0.0	0.0	0.0	0.0	3.3832520901731414E-5
3	0.0	0.0	0.0	0.0	3.3832520901731414E-5
4	0.0	1.6916260450865707E-5	1.6916260450865707E-5	0.0	3.3832520901731414E-5
5	1.6916260450865707E-5	1.6916260450865707E-5	1.6916260450865707E-5	0.0	3.3832520901731414E-5
6	3.3832520901731414E-5	1.6916260450865707E-5	1.6916260450865707E-5	0.0	3.3832520901731414E-5
7	3.3832520901731414E-5	1.6916260450865707E-5	1.6916260450865707E-5	0.0	3.3832520901731414E-5
8	3.3832520901731414E-5	1.6916260450865707E-5	1.6916260450865707E-5	0.0	5.074878135259712E-5
9	3.3832520901731414E-5	1.6916260450865707E-5	1.6916260450865707E-5	3.3832520901731414E-5	1.0149756270519424E-4
10-11	8.458130225432854E-5	1.6916260450865707E-5	1.6916260450865707E-5	3.3832520901731414E-5	1.0149756270519424E-4
12-13	1.1841382315605995E-4	1.6916260450865707E-5	1.6916260450865707E-5	3.3832520901731414E-5	1.0995569293062709E-4
14-15	1.268719533814928E-4	2.537439067629856E-5	1.6916260450865707E-5	3.3832520901731414E-5	1.3533008360692566E-4
16-17	1.437882138323585E-4	5.074878135259712E-5	1.6916260450865707E-5	4.229065112716427E-5	1.3533008360692566E-4
18-19	1.5224634405779135E-4	5.074878135259712E-5	1.6916260450865707E-5	5.074878135259712E-5	1.3533008360692566E-4
20-21	1.6916260450865707E-4	5.074878135259712E-5	1.6916260450865707E-5	8.458130225432854E-5	1.3533008360692566E-4
22-23	1.8607886495952276E-4	5.9206911578029975E-5	1.6916260450865707E-5	2.1991138586125418E-4	1.9453699518495564E-4
24-25	2.2836951608668705E-4	6.766504180346283E-5	2.537439067629856E-5	5.582365948785682E-4	2.706601672138513E-4
26-27	2.537439067629856E-4	6.766504180346283E-5	3.3832520901731414E-5	0.001615502873057675	2.706601672138513E-4
28-29	2.537439067629856E-4	6.766504180346283E-5	3.3832520901731414E-5	0.005117168786386876	2.706601672138513E-4
30-31	2.706601672138513E-4	6.766504180346283E-5	3.3832520901731414E-5	0.01956365521142619	2.706601672138513E-4
32-33	2.706601672138513E-4	7.612317202889568E-5	3.3832520901731414E-5	0.05208516592821551	3.044926881155827E-4
34-35	2.87576427664717E-4	8.458130225432854E-5	3.3832520901731414E-5	0.09602515244933918	3.2140894856644845E-4
36-37	3.044926881155827E-4	8.458130225432854E-5	3.3832520901731414E-5	0.1654325690792412	3.2140894856644845E-4
38-39	3.044926881155827E-4	1.0995569293062709E-4	3.3832520901731414E-5	0.2879570435248615	3.298670787918813E-4
40-41	3.3832520901731414E-4	1.3533008360692566E-4	3.3832520901731414E-5	0.43224428704052054	3.3832520901731414E-4
42-43	3.3832520901731414E-4	1.3533008360692566E-4	3.3832520901731414E-5	0.5701963910173304	3.46783339242747E-4
44-45	3.3832520901731414E-4	1.3533008360692566E-4	3.3832520901731414E-5	0.7329561909453348	3.5524146946817984E-4
46-47	3.5524146946817984E-4	1.437882138323585E-4	3.3832520901731414E-5	0.9104500537260432	3.806158601444784E-4
48-49	3.7215772991904553E-4	1.5224634405779135E-4	3.3832520901731414E-5	1.107786690015617	3.890739903699113E-4
50-51	3.7215772991904553E-4	1.5224634405779135E-4	4.229065112716427E-5	1.3288145490666285	3.890739903699113E-4
52-53	3.806158601444784E-4	1.6916260450865707E-4	5.074878135259712E-5	1.5657267766810028	4.144483810462098E-4
54-55	3.890739903699113E-4	1.6916260450865707E-4	5.074878135259712E-5	1.833570386529785	4.482809019479412E-4
56-57	3.975321205953441E-4	1.6916260450865707E-4	5.074878135259712E-5	2.1154544925527854	4.6519716239880695E-4
58-59	4.0599025082077697E-4	1.7762073473408992E-4	5.074878135259712E-5	2.43346327276861	4.8211342284967265E-4
60-61	4.3982277172250836E-4	1.8607886495952276E-4	5.074878135259712E-5	2.786683249112911	4.990296833005383E-4
62-63	4.5673903217337405E-4	1.9453699518495564E-4	5.074878135259712E-5	3.1697688832832163	5.074878135259712E-4
64-65	4.5673903217337405E-4	2.0299512541038849E-4	5.074878135259712E-5	3.5582423464071216	5.074878135259712E-4
66-67	4.5673903217337405E-4	2.1991138586125418E-4	5.074878135259712E-5	3.996762566074913	5.074878135259712E-4
68-69	4.6519716239880695E-4	2.1991138586125418E-4	5.074878135259712E-5	4.475729564480725	5.074878135259712E-4
70-71	4.736552926242398E-4	2.1991138586125418E-4	5.074878135259712E-5	5.006527984907989	5.244040739768369E-4
72-73	4.736552926242398E-4	2.1991138586125418E-4	5.074878135259712E-5	5.542917229414265	6.174435064565983E-4
74-75	4.736552926242398E-4	2.1991138586125418E-4	5.074878135259712E-5	6.091503097705614	6.428178971328969E-4
76-77	4.736552926242398E-4	2.1991138586125418E-4	5.074878135259712E-5	6.691497481507144	6.428178971328969E-4
78-79	5.582365948785683E-4	2.1991138586125418E-4	5.074878135259712E-5	7.359199197763264	6.428178971328969E-4
80-81	5.582365948785683E-4	2.1991138586125418E-4	5.074878135259712E-5	8.042607661848013	6.681922878091955E-4
82-83	5.666947251040011E-4	2.1991138586125418E-4	5.074878135259712E-5	8.756761429302212	6.93566678485494E-4
84-85	5.75152855329434E-4	2.1991138586125418E-4	5.074878135259712E-5	9.492221226794275	6.93566678485494E-4
86	5.75152855329434E-4	2.1991138586125418E-4	5.074878135259712E-5	10.061436474705454	6.93566678485494E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	16280	0.0	40.277576	1
GGTATCA	9715	0.0	36.66073	1
TATCAAC	20295	0.0	31.810581	2
TCAACGC	21145	0.0	30.357735	4
ATATGTA	4405	0.0	30.293886	1
ATCAACG	21410	0.0	29.982239	3
CAACGCA	21595	0.0	29.574686	5
AACGCAG	22650	0.0	28.41169	6
GTACATG	37480	0.0	28.385147	1
TATGTAA	4780	0.0	28.262056	2
TACATGG	38455	0.0	27.279562	2
GTAAGTA	8640	0.0	26.219082	2
TGTAAGT	8520	0.0	26.03217	1
ACATGGG	40550	0.0	25.496231	3
ATTATAT	3065	0.0	25.071995	1
CATGGGG	35140	0.0	24.334658	4
ATGGGGG	27555	0.0	24.211672	5
TGGGGGG	25455	0.0	23.58587	6
ACGCAGA	26930	0.0	23.533892	7
CGCAGAG	27655	0.0	22.733044	8
>>END_MODULE