FastQCFastQC Report
Mon 15 Aug 2016
SRR2921572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5911472
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC90370.15287224569447339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA164450.039.6078221
GGTATCA104050.036.032591
TATCAAC215500.029.7933522
TCAACGC222950.028.7777124
GTACATG384350.028.4097331
CAACGCA228550.028.0102545
ATCAACG228950.027.9613173
ATATGTA46250.027.569851
TACATGG394400.027.2120672
AACGCAG239550.026.7197366
TATGTAA49300.026.4896222
ATTATAT35300.026.417561
ACATGGG415500.025.374083
GTAAGTA88750.024.5068282
TTATATG36850.024.4853382
CATGGGG356000.024.3589844
TGTAAGT87850.024.1006981
ATGGGGG285100.024.0701625
TGGGGGG254050.023.8676786
TATATGT36050.023.31723
Produced by FastQC (version 0.11.5)