##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921572_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5911472
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.02355893760471	31.0	30.0	34.0	26.0	34.0
2	29.999061316707582	31.0	30.0	34.0	26.0	34.0
3	30.168092481872534	31.0	30.0	34.0	26.0	34.0
4	33.83384189251002	35.0	35.0	37.0	30.0	37.0
5	33.345620515499355	35.0	33.0	37.0	27.0	37.0
6	33.243009693693885	35.0	33.0	37.0	27.0	37.0
7	33.05722229590194	35.0	33.0	37.0	26.0	37.0
8	32.95069451398907	35.0	33.0	37.0	26.0	37.0
9	34.22725321205954	37.0	34.0	39.0	26.0	39.0
10-11	34.00620209315041	37.0	33.0	39.0	25.5	39.0
12-13	33.78389164323201	37.0	33.0	39.0	25.0	39.0
14-15	34.58010077693001	38.0	33.0	40.0	25.0	41.0
16-17	34.42325913072074	38.0	33.0	40.0	24.0	41.0
18-19	34.29829939142061	38.0	33.0	40.0	23.0	41.0
20-21	34.15929501146246	38.0	33.0	40.0	22.0	41.0
22-23	34.01975362481629	38.0	33.0	40.0	21.5	41.0
24-25	33.84496746326465	38.0	32.5	40.0	19.5	41.0
26-27	33.74757133248707	38.0	32.5	40.0	19.5	41.0
28-29	33.750943589007946	38.0	32.5	40.0	19.0	41.0
30-31	33.56945021476884	38.0	32.0	40.0	18.0	41.0
32-33	33.385180543864536	38.0	32.0	40.0	18.0	41.0
34-35	33.194919725577655	38.0	32.0	40.0	16.5	41.0
36-37	33.04605967853692	37.5	31.5	40.0	16.0	41.0
38-39	32.87569280544676	37.0	31.0	40.0	15.5	41.0
40-41	32.89566794869366	37.0	31.5	40.0	15.0	41.0
42-43	32.705848052735426	37.0	31.0	40.0	14.0	41.0
44-45	32.52503817999984	37.0	31.0	40.0	10.5	41.0
46-47	32.25653162190399	37.0	31.0	40.0	9.0	41.0
48-49	31.986521377416658	37.0	30.0	40.0	8.0	41.0
50-51	31.73043702143899	36.0	30.0	40.0	7.0	41.0
52-53	31.426339750911445	36.0	30.0	39.0	2.0	41.0
54-55	31.12480825418779	35.5	29.0	39.0	2.0	40.5
56-57	30.809287094652568	35.0	29.0	39.0	2.0	40.0
58-59	30.336566002511727	35.0	28.0	39.0	2.0	40.0
60-61	29.958303194195963	35.0	28.0	38.0	2.0	40.0
62-63	29.57728827946745	34.0	27.5	38.0	2.0	40.0
64-65	29.158008783599076	34.0	26.5	37.5	2.0	40.0
66-67	28.764014445133125	34.0	26.0	37.0	2.0	39.0
68-69	28.332923593311445	34.0	26.0	36.5	2.0	39.0
70-71	27.87565981873889	33.0	26.0	36.0	2.0	39.0
72-73	27.39010461353788	33.0	25.0	35.5	2.0	38.0
74-75	26.84875095407709	32.5	24.5	35.0	2.0	37.0
76-77	25.377177376463933	30.5	21.5	34.0	2.0	35.5
78-79	26.269821543602	32.0	24.0	34.5	2.0	36.0
80-81	26.334528777265632	32.0	24.0	35.0	2.0	36.0
82-83	26.018298149767098	32.0	23.5	35.0	2.0	35.5
84-85	25.618815838085673	32.0	21.5	35.0	2.0	35.0
86-87	25.201247337380607	32.0	20.0	34.0	2.0	35.0
88-89	24.790014568283503	31.5	18.5	34.0	2.0	35.0
90-91	24.378555967109378	31.0	15.0	34.0	2.0	35.0
92-93	23.959162455645565	31.0	8.5	34.0	2.0	35.0
94-95	23.572415973551088	31.0	2.0	34.0	2.0	35.0
96-97	23.054214331049863	31.0	2.0	34.0	2.0	35.0
98-99	22.354570316834792	31.0	2.0	34.0	2.0	35.0
100-101	19.79992309867999	26.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	155153.0
3	72690.0
4	41009.0
5	28522.0
6	23829.0
7	23284.0
8	26173.0
9	29427.0
10	33218.0
11	36583.0
12	40752.0
13	44627.0
14	48460.0
15	52051.0
16	55282.0
17	57390.0
18	56684.0
19	55279.0
20	55428.0
21	57731.0
22	62770.0
23	71164.0
24	82283.0
25	96167.0
26	114066.0
27	134413.0
28	158395.0
29	184946.0
30	218435.0
31	255926.0
32	307163.0
33	376392.0
34	472841.0
35	561656.0
36	708423.0
37	768122.0
38	331527.0
39	13211.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.27407641242031	18.117335790028513	13.964287839545344	24.64429995800584
2	16.251417582625784	20.53676309386224	38.801283335182845	24.410535988329134
3	20.316692855870514	25.012090903293245	28.416929869715972	26.254286371120273
4	15.45080301054727	15.597906144647636	34.22400516747341	34.727285677331686
5	15.257829458151248	38.71823763384647	31.441557584647477	14.582375323354805
6	34.53737456939457	32.448385773687576	17.710884465487045	15.303355191430803
7	30.084527122096954	32.6675016948452	19.14516107720668	18.102810105851173
8	28.953948140892738	34.4200977718635	19.020902196452894	17.605051890790865
9	30.34755475395122	15.533229181220442	17.567226801810385	36.55198926301795
10-11	27.779682717816577	25.10354635851069	25.573894825912802	21.542876097759923
12-13	27.762187449572256	21.556406179967343	26.526082224931834	24.15532414552857
14-15	25.52670722717455	23.08893573760691	24.06524175618051	27.31911527903803
16-17	25.532442027164436	26.000861505864513	24.607427826029987	23.859268640941064
18-19	25.632666314699833	26.292946958733115	25.68713737279188	22.387249353775175
20-21	25.797724286514384	24.876506652688384	26.031559796506997	23.294209264290235
22-23	25.953399335732207	24.025277255109238	25.539036144659903	24.48228726449865
24-25	25.449857386911084	24.33726622173629	25.209543518766885	25.003332872585744
26-27	24.75860639090109	24.94095694265791	25.711292397264913	24.58914426917609
28-29	24.746165624709196	26.4554298814791	24.61819519994586	24.180209293865847
30-31	26.681028268343148	24.972375026821183	24.766571091874315	23.58002561296135
32-33	26.000445920315645	24.89890427986196	24.75275749254014	24.34789230728225
34-35	25.61424200667959	25.65943772164178	25.35683544359497	23.36948482808366
36-37	25.385879959287582	25.790161143027625	25.59258965620615	23.231369241478642
38-39	25.97899084044507	25.515534334729267	24.865106174972198	23.64036864985346
40-41	26.009651327376883	25.063223630650988	24.670383930483236	24.256741111488896
42-43	25.044347235597325	25.305701067058028	24.55820711102402	25.091744586320626
44-45	24.403720963913802	25.228123153679512	25.54991164732563	24.818244235081053
46-47	24.4181464906206	24.620206603546503	25.313989756545567	25.64765714928733
48-49	24.28416015839097	25.25552115150129	25.673709723911976	24.78660896619576
50-51	24.825224577112845	24.677976706310627	25.98459641427832	24.512202302298203
52-53	24.831724038758413	24.830615930535995	25.52560279818473	24.81205723252086
54-55	23.277975626208754	24.39373869919258	26.59219673907361	25.73608893552506
56-57	24.281965494687913	24.4261915951965	25.460939850018388	25.8309030600972
58-59	24.677466928311766	25.080304963889304	25.726948658366805	24.51527944943213
60-61	25.958477043161388	24.597188737520884	25.77399825454164	23.670335964776093
62-63	25.27591468869332	24.498987718542697	25.862253757343616	24.362843835420367
64-65	24.28900754471849	24.075728207410517	26.307260134554124	25.328004113316872
66-67	24.75170333954925	24.613770624106184	25.513278457022253	25.121247579322315
68-69	23.824627393141057	24.83995219770202	25.82214585863244	25.513274550524486
70-71	24.193309159126294	24.402456634621757	25.684255930717264	25.71997827553469
72-73	23.886850597790115	25.569174911442026	25.054596327386196	25.489378163381666
74-75	23.690849353850922	24.915745992169747	25.658516598035312	25.734888055944015
76-77	23.449714235929015	23.972290089093843	25.787321628402633	26.79067404657451
78-79	23.294566501133346	24.347499948578555	26.303639444844766	26.054294105443333
80-81	24.06760958285463	24.660571913326695	25.410323181981337	25.861495321837342
82-83	23.929431528941244	24.808238402058585	25.72185411416792	25.540475954832253
84-85	23.84364537000023	25.04734167094969	25.077468765664758	26.03154419338532
86-87	22.996945818675893	25.606602230266585	25.28524769165689	26.11120425940064
88-89	22.904169959407174	24.612410340388145	25.913915905303558	26.569503794901124
90-91	23.90723811465656	23.752638364994844	26.069327657325264	26.270795863023334
92-93	24.234943520549848	23.862764884982628	25.573093692394412	26.32919790207311
94-95	23.69458287661923	24.530561056518756	25.267204711758595	26.507651355103416
96-97	22.5998372055589	24.31725681131823	25.85974386816205	27.223162114960825
98-99	22.11765760713496	23.905786960381413	25.98511041158889	27.99144502089474
100-101	22.775644995747875	24.344185738068802	25.82325170190705	27.05691756427627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	226.0
1	212.5
2	263.5
3	353.0
4	337.5
5	357.0
6	552.0
7	1144.0
8	1619.5
9	1639.0
10	1458.5
11	1217.0
12	1216.5
13	1360.5
14	1561.5
15	1704.0
16	1935.0
17	2235.5
18	2432.5
19	2602.5
20	2745.5
21	2884.5
22	3023.0
23	3201.5
24	3346.5
25	3592.0
26	4086.0
27	4761.0
28	5762.0
29	7384.5
30	10083.5
31	14004.5
32	19910.0
33	27530.0
34	35937.0
35	45859.5
36	58044.5
37	70950.0
38	83660.0
39	97096.0
40	111392.5
41	125214.0
42	140152.0
43	158062.5
44	176649.5
45	197237.0
46	221872.0
47	249704.0
48	278964.0
49	303266.0
50	322813.5
51	335162.0
52	336602.0
53	331673.0
54	320420.5
55	303670.0
56	282036.0
57	252193.0
58	213864.5
59	172967.0
60	136952.5
61	108429.0
62	84612.0
63	63629.5
64	45622.5
65	32160.0
66	23023.5
67	16651.0
68	12076.5
69	8434.5
70	5867.0
71	4187.5
72	2799.5
73	1881.0
74	1284.5
75	775.0
76	447.5
77	240.5
78	137.5
79	78.5
80	38.5
81	18.5
82	12.5
83	9.0
84	4.5
85	4.5
86	3.0
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22046962245613275
2	0.0
3	5.074878135259712E-5
4	0.011046318074415307
5	0.013228515672576983
6	0.10850089453185265
7	0.1400328040122663
8	0.15842077912235736
9	0.11244238321690436
10-11	0.12234685371088623
12-13	0.09718391629022348
14-15	0.12051989758219271
16-17	0.07398326508186116
18-19	0.07892281313351394
20-21	0.05513009280937133
22-23	0.08150254285227097
24-25	0.07452458541628887
26-27	0.05196675210505945
28-29	0.018980044225871323
30-31	0.033223535525500246
32-33	0.039660172627054645
34-35	0.04611372598905991
36-37	0.03752872381024557
38-39	0.0386959457813553
40-41	0.021999596716350853
42-43	0.03144732817815935
44-45	0.018066566161524574
46-47	0.031202042401621794
48-49	4.0599025082077697E-4
50-51	0.017423748264391678
52-53	0.008407381444080257
54-55	0.015952033605166362
56-57	0.010386583916831544
58-59	0.006961041175531239
60-61	0.031286623703876124
62-63	0.013761377876779252
64-65	0.015453003921865825
66-67	0.05952832052659642
68-69	0.07854219727336947
70-71	0.03392556033421117
72-73	0.05644110299431342
74-75	0.04822825854541813
76-77	0.2004576863427586
78-79	0.07449075289538715
80-81	0.016552560851172092
82-83	0.010437332698184141
84-85	0.025535095150581784
86-87	0.16731027398928727
88-89	0.3764544600735654
90-91	0.27487231606611684
92-93	0.27605645429767744
94-95	0.0022583207701905718
96-97	0.0056923216417163105
98-99	0.004981838702779951
100-101	0.005227124479317503
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5911472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.74487575907914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.45643196087207	34.33711785716215
2	12.616105226510724	11.794765427534257
3	5.080588045953905	7.1247437097353385
4	2.5484716982188744	4.765119716350829
5	1.540100464036929	3.599590242395318
6	1.0022824314150816	2.8110940639203448
7	0.6571317828418314	2.150228048239851
8	0.4898979695176181	1.8320175775780958
9	0.35763668845867497	1.5045914312000328
>10	2.039178054983861	17.44126368947481
>50	0.14281329305912915	4.5763388159648875
>100	0.06390544146280268	5.090289226279658
>500	0.003528837359907994	1.1056895253752876
>1k	0.0018553466178268623	1.6184285208422546
>5k	7.275869087527914E-5	0.24872214794664288
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9037	0.15287224569447339	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.6916260450865707E-5	0.0	0.0	0.0
4	0.0	1.6916260450865707E-5	0.0	0.0	0.0
5	0.0	1.6916260450865707E-5	0.0	0.0	0.0
6	0.0	1.6916260450865707E-5	0.0	0.0	0.0
7	0.0	1.6916260450865707E-5	0.0	0.0	0.0
8	0.0	1.6916260450865707E-5	0.0	0.0	3.3832520901731414E-5
9	0.0	1.6916260450865707E-5	0.0	3.3832520901731414E-5	3.3832520901731414E-5
10-11	8.458130225432854E-5	1.6916260450865707E-5	0.0	4.229065112716427E-5	3.3832520901731414E-5
12-13	1.0149756270519424E-4	1.6916260450865707E-5	0.0	5.074878135259712E-5	3.3832520901731414E-5
14-15	1.0149756270519424E-4	3.3832520901731414E-5	0.0	5.074878135259712E-5	6.766504180346283E-5
16-17	1.0995569293062709E-4	3.3832520901731414E-5	0.0	5.9206911578029975E-5	1.0149756270519424E-4
18-19	1.1841382315605995E-4	3.3832520901731414E-5	0.0	7.612317202889568E-5	1.1841382315605995E-4
20-21	1.268719533814928E-4	3.3832520901731414E-5	0.0	1.0995569293062709E-4	1.268719533814928E-4
22-23	1.8607886495952276E-4	3.3832520901731414E-5	0.0	2.452857765375528E-4	1.7762073473408992E-4
24-25	2.1991138586125418E-4	5.074878135259712E-5	0.0	5.920691157802998E-4	2.1991138586125418E-4
26-27	2.1991138586125418E-4	6.766504180346283E-5	0.0	0.001657793524184839	2.368276463121199E-4
28-29	2.1991138586125418E-4	6.766504180346283E-5	0.0	0.005294789521120966	2.368276463121199E-4
30-31	2.368276463121199E-4	6.766504180346283E-5	0.0	0.019589029602102487	2.368276463121199E-4
32-33	2.6220203698841847E-4	6.766504180346283E-5	0.0	0.051916003323706855	2.706601672138513E-4
34-35	3.129508183410156E-4	6.766504180346283E-5	0.0	0.09594057114708485	2.87576427664717E-4
36-37	3.5524146946817984E-4	7.612317202889568E-5	0.0	0.16642217031561682	2.87576427664717E-4
38-39	3.5524146946817984E-4	8.458130225432854E-5	0.0	0.29065518706677457	3.298670787918813E-4
40-41	4.0599025082077697E-4	8.458130225432854E-5	0.0	0.43806348063561834	3.636995996936127E-4
42-43	4.0599025082077697E-4	1.0149756270519424E-4	8.458130225432854E-6	0.5784853586382546	3.7215772991904553E-4
44-45	4.0599025082077697E-4	1.0149756270519424E-4	1.6916260450865707E-5	0.7433596911226171	3.7215772991904553E-4
46-47	4.0599025082077697E-4	1.0149756270519424E-4	1.6916260450865707E-5	0.9228073819854006	3.806158601444784E-4
48-49	4.0599025082077697E-4	1.0149756270519424E-4	1.6916260450865707E-5	1.1202539739679052	3.890739903699113E-4
50-51	4.0599025082077697E-4	1.0149756270519424E-4	1.6916260450865707E-5	1.3403852712150206	3.975321205953441E-4
52-53	4.313646414970755E-4	1.0149756270519424E-4	1.6916260450865707E-5	1.5751660500125857	4.6519716239880695E-4
54-55	4.3982277172250836E-4	1.0149756270519424E-4	1.6916260450865707E-5	1.839964732980212	5.328622042022697E-4
56-57	4.3982277172250836E-4	1.0995569293062709E-4	1.6916260450865707E-5	2.1186432076477737	5.497784646531355E-4
58-59	4.3982277172250836E-4	1.1841382315605995E-4	1.6916260450865707E-5	2.4322199276254715	5.582365948785683E-4
60-61	4.3982277172250836E-4	1.1841382315605995E-4	1.6916260450865707E-5	2.7817944498426113	5.582365948785683E-4
62-63	4.3982277172250836E-4	1.1841382315605995E-4	1.6916260450865707E-5	3.161665994527252	5.920691157802997E-4
64-65	4.3982277172250836E-4	1.1841382315605995E-4	1.6916260450865707E-5	3.5467223730400823	6.005272460057325E-4
66-67	4.3982277172250836E-4	1.5224634405779135E-4	1.6916260450865707E-5	3.980336877177123	6.259016366820312E-4
68-69	5.582365948785683E-4	1.5224634405779135E-4	1.6916260450865707E-5	4.452393583188755	6.597341575837626E-4
70-71	5.666947251040011E-4	1.5224634405779135E-4	1.6916260450865707E-5	4.977212105546639	7.273991993872254E-4
72-73	5.75152855329434E-4	1.5224634405779135E-4	1.6916260450865707E-5	5.508526471917654	7.950642411906882E-4
74-75	5.75152855329434E-4	1.5224634405779135E-4	3.3832520901731414E-5	6.055733664982258	7.950642411906882E-4
76-77	5.75152855329434E-4	1.607044742832242E-4	3.3832520901731414E-5	6.656912187015349	7.950642411906882E-4
78-79	5.920691157802997E-4	1.6916260450865707E-4	3.3832520901731414E-5	7.326017952888892	7.950642411906882E-4
80-81	5.920691157802997E-4	1.6916260450865707E-4	3.3832520901731414E-5	8.007548712063594	8.119805016415539E-4
82-83	6.259016366820312E-4	1.6916260450865707E-4	3.3832520901731414E-5	8.725060357217288	8.288967620924196E-4
84-85	6.597341575837626E-4	1.6916260450865707E-4	3.3832520901731414E-5	9.464216357617865	8.288967620924196E-4
86-87	6.681922878091955E-4	1.6916260450865707E-4	3.3832520901731414E-5	10.226471511664101	8.373548923178524E-4
88-89	6.766504180346283E-4	1.6916260450865707E-4	3.3832520901731414E-5	11.052670130214608	8.62729282994151E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	16445	0.0	39.607822	1
GGTATCA	10405	0.0	36.03259	1
TATCAAC	21550	0.0	29.793352	2
TCAACGC	22295	0.0	28.777712	4
GTACATG	38435	0.0	28.409733	1
CAACGCA	22855	0.0	28.010254	5
ATCAACG	22895	0.0	27.961317	3
ATATGTA	4625	0.0	27.56985	1
TACATGG	39440	0.0	27.212067	2
AACGCAG	23955	0.0	26.719736	6
TATGTAA	4930	0.0	26.489622	2
ATTATAT	3530	0.0	26.41756	1
ACATGGG	41550	0.0	25.37408	3
GTAAGTA	8875	0.0	24.506828	2
TTATATG	3685	0.0	24.485338	2
CATGGGG	35600	0.0	24.358984	4
TGTAAGT	8785	0.0	24.100698	1
ATGGGGG	28510	0.0	24.070162	5
TGGGGGG	25405	0.0	23.867678	6
TATATGT	3605	0.0	23.3172	3
>>END_MODULE