FastQCFastQC Report
Mon 15 Aug 2016
SRR2921571_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921571_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6178948
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135400.039.0474931
GGTATCA80350.037.1664121
TATCAAC175800.029.5617162
TCAACGC174150.029.4191634
CAACGCA178250.028.715775
ATCAACG183150.028.1995263
AACGCAG188100.027.3324726
GTACATG293050.026.7716081
TACATGG303950.025.337152
ACATGGG315900.023.7239363
ACGCAGA221850.022.5291237
CATGGGG251150.021.9180814
CGCAGAG231950.021.3474838
GTATATG17250.020.2729191
ATGGGGG164950.019.2760755
GTAAGTA40400.019.1079392
GCAGAGT251700.018.4129189
TGTAAGT41700.018.0967081
ATATGTA25600.017.9742951
GAGTACA232850.017.8049341
Produced by FastQC (version 0.11.5)