##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921571_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6178948
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.000344557034627	31.0	28.0	31.0	16.0	34.0
2	27.982196969451756	31.0	28.0	33.0	16.0	34.0
3	28.069375078087727	31.0	28.0	33.0	16.0	34.0
4	31.47629159526832	35.0	32.0	37.0	17.0	37.0
5	31.396388996961942	35.0	32.0	37.0	17.0	37.0
6	31.49056748818731	35.0	32.0	37.0	17.0	37.0
7	31.286939135917635	35.0	32.0	37.0	17.0	37.0
8	31.230864056470455	35.0	32.0	37.0	17.0	37.0
9	32.434229904508015	37.0	32.0	39.0	17.0	39.0
10-11	32.201467628470084	37.0	32.0	39.0	13.0	39.0
12-13	31.98365304255676	37.0	32.0	39.0	10.5	39.0
14-15	32.65248995460068	38.0	31.0	40.0	10.0	41.0
16-17	32.492265350023985	37.5	31.0	40.0	10.0	41.0
18-19	32.303036050797	37.0	31.0	40.0	10.0	41.0
20-21	32.17042205242705	37.0	31.0	40.0	8.5	41.0
22-23	31.942341074888475	37.0	30.0	40.0	8.0	41.0
24-25	32.03851351395092	37.0	30.5	40.0	5.0	41.0
26-27	31.778502262844743	37.0	30.0	40.0	2.0	41.0
28-29	31.569620508215962	37.0	30.0	40.0	2.0	41.0
30-31	31.301571885699637	37.0	30.0	40.0	2.0	41.0
32-33	31.0465105063192	36.0	29.5	40.0	2.0	41.0
34-35	30.698557667098022	36.0	28.5	39.5	2.0	41.0
36-37	30.392831595281265	36.0	28.0	39.0	2.0	41.0
38-39	30.164596384368345	35.5	27.0	39.0	2.0	40.0
40-41	29.92164887938853	35.0	27.0	39.0	2.0	40.0
42-43	29.659193441990446	35.0	26.5	39.0	2.0	40.0
44-45	29.387971382830862	35.0	26.0	39.0	2.0	40.0
46-47	29.074476027310798	35.0	25.0	39.0	2.0	40.0
48-49	28.77341798312593	34.5	24.0	39.0	2.0	40.0
50-51	27.860239073059038	33.5	23.5	37.5	2.0	39.5
52-53	28.06777779971607	33.5	24.0	38.0	2.0	39.5
54-55	28.624341392741936	34.5	24.5	38.5	2.0	40.0
56-57	28.591555390982414	34.5	25.0	39.0	2.0	40.0
58-59	28.325340899454083	34.0	24.0	38.5	2.0	40.0
60-61	27.955156605946513	34.0	23.0	38.0	2.0	40.0
62-63	27.505630408283093	34.0	22.5	38.0	2.0	40.0
64-65	27.030114996921807	33.0	21.5	37.0	2.0	40.0
66-67	26.67130561707268	33.0	20.5	36.5	2.0	39.0
68-69	26.200025716351718	32.5	19.0	36.0	2.0	39.0
70-71	25.761005756967045	32.0	18.0	35.5	2.0	39.0
72-73	25.272471867379366	32.0	13.5	35.0	2.0	38.0
74-75	24.813055312975607	31.5	9.5	35.0	2.0	37.0
76-77	24.379318453561996	31.0	7.0	35.0	2.0	36.5
78-79	23.971435266974247	31.0	2.0	34.5	2.0	36.0
80-81	23.524937578370945	31.0	2.0	34.0	2.0	35.5
82-83	22.976928758746634	30.0	2.0	34.0	2.0	35.0
84-85	22.55549277967706	30.0	2.0	34.0	2.0	35.0
86-87	22.056464789799172	29.5	2.0	34.0	2.0	35.0
88-89	21.534980873766862	29.0	2.0	34.0	2.0	35.0
90-91	20.929322758501932	29.0	2.0	34.0	2.0	35.0
92-93	20.362898587267605	29.0	2.0	34.0	2.0	35.0
94-95	19.666763015322353	27.0	2.0	33.5	2.0	35.0
96-97	19.01683053490659	26.5	2.0	33.0	2.0	35.0
98	18.53911135034637	25.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	400326.0
3	69363.0
4	50622.0
5	40875.0
6	37509.0
7	39234.0
8	45028.0
9	50913.0
10	53635.0
11	55604.0
12	56529.0
13	55655.0
14	54307.0
15	56372.0
16	60201.0
17	64071.0
18	66784.0
19	67213.0
20	69937.0
21	74511.0
22	80068.0
23	88881.0
24	99756.0
25	113447.0
26	128922.0
27	148021.0
28	168912.0
29	193622.0
30	223347.0
31	259781.0
32	303408.0
33	364517.0
34	443437.0
35	534071.0
36	638753.0
37	622573.0
38	281205.0
39	17538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.015948976257135	17.16694971001714	13.24344313554699	24.573658178178736
2	16.265136579647503	18.73949315651425	39.42973058727482	25.56563967656342
3	20.478078456086017	22.947176047917388	27.683381998181527	28.891363497815064
4	14.194917863311849	14.627849020160678	34.669395374160246	36.50783774236723
5	15.3942649687304	36.83052940956299	32.5360207620362	15.239184859670404
6	35.2123161414993	32.622895478430806	16.858030353197755	15.306758026872142
7	31.101151448392695	30.90220103365498	19.105747916921647	18.890899601030675
8	28.321819977284594	34.01402385521837	18.694157363640823	18.96999880385621
9	28.255167005617167	15.870037234649509	18.271943234356552	37.602852525376775
10-11	27.181167267289965	24.74108083709306	25.797748419246986	22.280003476369988
12-13	27.29921092131257	21.59061003807013	26.466959009147384	24.643220031469923
14-15	24.789887641449678	23.179275080699767	23.71957729806485	28.311259979785707
16-17	24.94498575047383	26.23466558465704	24.454982025535934	24.365366639333192
18-19	25.042146447490026	25.86428552461398	25.729264692643426	23.364303335252565
20-21	25.48742497743107	24.855049374654662	25.731645702491164	23.925879945423112
22-23	25.48746914048709	24.242154509726866	25.49265272062228	24.777723629163763
24-25	25.11613892588114	24.695439483299396	25.307224606407065	24.881196984412405
26-27	24.749754599443367	24.89648068755725	25.548228609254853	24.805536103744526
28-29	24.72035910150109	25.38832871524181	25.272027324630642	24.619284858626465
30-31	25.51278477898619	24.987290610891222	25.08970261730009	24.410221992822496
32-33	25.20524123918799	24.99807376414974	24.99336338908734	24.80332160757493
34-35	25.271916061272726	25.173268387238334	25.314119446372086	24.240696105116857
36-37	25.218011634488107	25.049379857533598	25.407547774469048	24.325060733509247
38-39	25.35361027178681	25.061699977793932	25.10068846920644	24.484001281212816
40-41	25.32296376559143	24.893676911122153	25.11655696273238	24.66680236055403
42-43	24.95530919027144	24.88308309483218	25.02859051075084	25.133017204145542
44-45	24.705953772138837	24.89631839717141	25.373771965156845	25.02395586553291
46-47	24.96139909922574	24.66544803828534	25.208319873879915	25.164832988609
48-49	24.760790120787608	24.969811416612515	25.218534990797952	25.050863471801925
50-51	24.854025448368812	24.950848099058092	25.418323763743967	24.776802688829125
52-53	24.924022498596557	24.90872550552746	25.369177987984227	24.798074007891753
54-55	24.232837866252304	24.66329265193823	25.835446151747853	25.268423330061612
56-57	24.670210740114033	24.761788879352093	25.3437427097486	25.224257670785278
58-59	24.864778048689235	24.76041811819543	25.590768995288748	24.78403483782658
60-61	25.304820492209227	24.628706864408308	25.653870858669098	24.41260178471337
62-63	24.92685801579901	24.655481714443077	25.7169058848709	24.70075438488701
64-65	24.542102746726	24.4372974170856	25.910570880986572	25.11002895520183
66-67	24.768947596186244	24.723626168861696	25.437856788511642	25.069569446440415
68-69	24.41255104145218	24.68648733275963	25.646684678567595	25.254276947220593
70-71	24.4706918891409	24.401492588664038	25.756007226328453	25.37180829586661
72-73	24.306590609141978	24.892988834686356	25.408136880827463	25.392283675344203
74-75	24.18790635508589	24.785345221377355	25.738301058016884	25.288447365519872
76-77	24.206484768309057	24.25189837897423	25.724814774042137	25.816802078674577
78-79	24.12034221928174	24.33445846041888	25.97036430671227	25.574835013587116
80-81	24.4718435684307	24.5533957482378	25.581254988510256	25.393505694821243
82-83	24.38360300845379	24.46532413504844	25.789566598134396	25.361506258363377
84-85	24.278382070176445	24.55671838643789	25.524486700294176	25.640412843091493
86-87	23.984103923975685	24.739036930901197	25.688781153468977	25.588077991654146
88-89	23.82486620570086	24.279213868277395	25.938637223144447	25.957282702877304
90-91	24.196576205620417	23.921844200140864	26.080662582368895	25.800917011869817
92-93	24.429689563447177	23.934875944136085	25.89441055861966	25.74102393379707
94-95	24.241019226801686	24.0614958448304	25.818477100100267	25.879007828267646
96-97	23.831858459490032	23.897345308210465	26.101514837695728	26.16928139460378
98	23.751733089950307	23.66297941385255	25.904228780042594	26.68105871615455
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	169.0
1	164.5
2	207.5
3	298.5
4	337.0
5	370.0
6	559.0
7	1105.0
8	1699.5
9	1914.0
10	1854.0
11	1817.5
12	2011.5
13	2320.5
14	2600.0
15	2841.5
16	3026.0
17	3104.5
18	3118.5
19	3030.0
20	2894.5
21	2835.5
22	2832.5
23	2904.5
24	3111.0
25	3590.0
26	4356.0
27	5419.0
28	6876.0
29	9546.5
30	13912.0
31	20178.5
32	29258.5
33	39393.5
34	50898.5
35	65512.0
36	81348.5
37	95970.0
38	110731.0
39	124447.5
40	137314.0
41	152574.0
42	170682.5
43	190721.0
44	209839.5
45	230400.5
46	253284.0
47	276188.0
48	294618.0
49	307752.5
50	313890.0
51	314836.5
52	316414.5
53	316528.0
54	312227.5
55	302827.0
56	285681.5
57	261091.0
58	233254.5
59	201513.5
60	169208.0
61	137728.5
62	109028.5
63	83312.5
64	59687.5
65	42483.5
66	30990.0
67	22411.5
68	15660.0
69	10645.5
70	7206.5
71	4869.5
72	3262.5
73	2163.0
74	1436.5
75	952.5
76	577.0
77	351.5
78	203.0
79	114.5
80	79.0
81	57.0
82	47.0
83	35.0
84	25.5
85	26.5
86	19.5
87	14.5
88	11.5
89	13.0
90	16.0
91	12.5
92	9.5
93	11.5
94	12.5
95	10.0
96	7.5
97	8.0
98	9.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.26169503287614654
2	0.12898635819560222
3	0.12667204838105128
4	0.12183303695062656
5	0.1181269044504016
6	0.043097951301742626
7	0.03288585694522757
8	0.012316012369743199
9	0.009111583395749568
10-11	0.0014403746398254202
12-13	0.005664394650998843
14-15	0.010462946119630721
16-17	0.005203151086560366
18-19	0.046350932229887676
20-21	0.09126148981994993
22-23	0.09076787828607717
24-25	0.06847443933821744
26-27	0.0786298897482225
28-29	0.09326830392487524
30-31	0.0708696690763541
32-33	0.01773764724998495
34-35	0.27706981835742917
36-37	0.36152594260382187
38-39	0.3353402553314901
40-41	0.3314237310299423
42-43	0.30951061572293537
44-45	0.2935693907765529
46-47	0.29891819772556755
48-49	0.2928087434948473
50-51	0.29454042985958123
52-53	0.3378406809702882
54-55	0.3255084846158278
56-57	0.31042501085945373
58-59	0.29225848801446463
60-61	0.29971121297670733
62-63	0.29937944129000604
64-65	0.31450337500817294
66-67	0.33521887544611156
68-69	0.29764775492527207
70-71	0.2757103636411894
72-73	0.26147654908246515
74-75	0.24904724881970197
76-77	0.25242970162558415
78-79	0.257980808383563
80-81	0.24938711249876191
82-83	0.25756811677327596
84-85	0.2515153064890658
86-87	0.24742885034798803
88-89	0.26859750235800656
90-91	0.2535868565328597
92-93	0.24457237704541293
94-95	0.25833685604734014
96-97	0.25697740133110036
98	0.25607919017929914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	6178948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.31710747846046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.99932244406119	33.98752356009233
2	10.730236210131013	9.725265352075505
3	4.76922045151031	6.483818273686932
4	2.563599988374843	4.646997448198508
5	1.5710633894163522	3.5598024236827603
6	1.0433137555274954	2.836797695579743
7	0.7344860418794104	2.3299348030894773
8	0.5316841965209196	1.9275511902669924
9	0.4017058213534546	1.6383731292897965
>10	2.385561359945833	19.851414739031036
>50	0.18469605117619642	5.758147441234813
>100	0.082947345681887	6.383257482621425
>500	0.0018024596275343688	0.530704223181937
>1k	3.6048479349253963E-4	0.3404122379687288
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.6183984717139553E-5	0.0
4	0.0	0.0	0.0	1.6183984717139553E-5	0.0
5	0.0	0.0	0.0	1.6183984717139553E-5	0.0
6	0.0	0.0	0.0	1.6183984717139553E-5	0.0
7	0.0	0.0	0.0	1.6183984717139553E-5	0.0
8	0.0	0.0	0.0	1.6183984717139553E-5	0.0
9	0.0	0.0	0.0	3.2367969434279106E-5	0.0
10-11	0.0	0.0	0.0	4.045996179284888E-5	0.0
12-13	0.0	0.0	0.0	6.473593886855821E-5	0.0
14-15	0.0	0.0	0.0	7.282793122712799E-5	0.0
16-17	0.0	0.0	0.0	1.4565586245425598E-4	0.0
18-19	0.0	0.0	0.0	2.670357478328026E-4	0.0
20-21	0.0	0.0	0.0	2.9131172490851195E-4	0.0
22-23	0.0	0.0	0.0	3.9650762556991904E-4	0.0
24-25	0.0	0.0	0.0	6.635433734027217E-4	0.0
26-27	0.0	0.0	8.091992358569776E-6	0.0010276830295383615	0.0
28-29	0.0	0.0	1.6183984717139553E-5	0.003123509050407934	0.0
30-31	0.0	0.0	1.6183984717139553E-5	0.010624785966802115	0.0
32-33	0.0	0.0	1.6183984717139553E-5	0.02699488650818877	0.0
34-35	0.0	0.0	1.6183984717139553E-5	0.0486733340367972	0.0
36-37	0.0	0.0	1.6183984717139553E-5	0.08641438639716664	0.0
38-39	0.0	0.0	1.6183984717139553E-5	0.15181386863912755	0.0
40-41	8.091992358569776E-6	1.6183984717139553E-5	1.6183984717139553E-5	0.22705321358910935	0.0
42-43	1.6183984717139553E-5	1.6183984717139553E-5	2.427597707570933E-5	0.30094119581520995	0.0
44-45	1.6183984717139553E-5	1.6183984717139553E-5	3.2367969434279106E-5	0.3909079668577887	0.0
46-47	1.6183984717139553E-5	1.6183984717139553E-5	3.2367969434279106E-5	0.4993325724702652	0.0
48-49	1.6183984717139553E-5	1.6183984717139553E-5	3.2367969434279106E-5	0.6190293234382294	0.0
50-51	1.6183984717139553E-5	1.6183984717139553E-5	3.2367969434279106E-5	0.7522477936373635	0.0
52-53	1.6183984717139553E-5	1.6183984717139553E-5	3.2367969434279106E-5	0.8932831284548761	0.0
54-55	1.6183984717139553E-5	1.6183984717139553E-5	3.2367969434279106E-5	1.0525659060409636	0.0
56-57	1.6183984717139553E-5	1.6183984717139553E-5	3.2367969434279106E-5	1.2353316454516206	0.0
58-59	1.6183984717139553E-5	1.6183984717139553E-5	3.2367969434279106E-5	1.439209392925786	1.6183984717139553E-5
60-61	1.6183984717139553E-5	1.6183984717139553E-5	3.2367969434279106E-5	1.664385264287707	1.6183984717139553E-5
62-63	1.6183984717139553E-5	2.427597707570933E-5	3.2367969434279106E-5	1.9038030422007113	1.6183984717139553E-5
64-65	2.427597707570933E-5	4.045996179284888E-5	3.2367969434279106E-5	2.155488280529307	1.6183984717139553E-5
66-67	4.045996179284888E-5	6.473593886855821E-5	3.2367969434279106E-5	2.4439111641658093	1.6183984717139553E-5
68-69	5.6643946509988435E-5	6.473593886855821E-5	3.2367969434279106E-5	2.7721304662217583	1.6183984717139553E-5
70-71	6.473593886855821E-5	6.473593886855821E-5	3.2367969434279106E-5	3.13925606753771	3.2367969434279106E-5
72-73	6.473593886855821E-5	6.473593886855821E-5	3.2367969434279106E-5	3.512102707451171	3.2367969434279106E-5
74-75	6.473593886855821E-5	6.473593886855821E-5	3.2367969434279106E-5	3.89406902275274	3.2367969434279106E-5
76-77	6.473593886855821E-5	6.473593886855821E-5	3.2367969434279106E-5	4.308629883274628	3.2367969434279106E-5
78-79	6.473593886855821E-5	7.282793122712799E-5	3.2367969434279106E-5	4.77986705827594	3.2367969434279106E-5
80-81	6.473593886855821E-5	8.091992358569776E-5	3.2367969434279106E-5	5.288303122149596	3.2367969434279106E-5
82-83	6.473593886855821E-5	8.091992358569776E-5	3.2367969434279106E-5	5.820076491985367	3.2367969434279106E-5
84-85	6.473593886855821E-5	8.091992358569776E-5	4.855195415141866E-5	6.3521330815536885	3.2367969434279106E-5
86	6.473593886855821E-5	8.091992358569776E-5	4.855195415141866E-5	6.76662111414435	3.2367969434279106E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	13540	0.0	39.047493	1
GGTATCA	8035	0.0	37.166412	1
TATCAAC	17580	0.0	29.561716	2
TCAACGC	17415	0.0	29.419163	4
CAACGCA	17825	0.0	28.71577	5
ATCAACG	18315	0.0	28.199526	3
AACGCAG	18810	0.0	27.332472	6
GTACATG	29305	0.0	26.771608	1
TACATGG	30395	0.0	25.33715	2
ACATGGG	31590	0.0	23.723936	3
ACGCAGA	22185	0.0	22.529123	7
CATGGGG	25115	0.0	21.918081	4
CGCAGAG	23195	0.0	21.347483	8
GTATATG	1725	0.0	20.272919	1
ATGGGGG	16495	0.0	19.276075	5
GTAAGTA	4040	0.0	19.107939	2
GCAGAGT	25170	0.0	18.412918	9
TGTAAGT	4170	0.0	18.096708	1
ATATGTA	2560	0.0	17.974295	1
GAGTACA	23285	0.0	17.804934	1
>>END_MODULE