FastQCFastQC Report
Mon 15 Aug 2016
SRR2921571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6178948
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129000.038.1742361
GGTATCA80600.037.8982961
TCAACGC169250.028.4539834
TATCAAC171650.028.4425832
CAACGCA173200.027.7225745
ATCAACG180950.026.7454193
AACGCAG186300.026.0247696
GTACATG291250.025.9093741
TACATGG303350.024.62662
ACATGGG312750.023.3101433
GTAAGTA40000.022.0837462
CATGGGG247300.021.4517574
TGTAAGT40900.021.2886181
ACGCAGA222850.021.2449877
ATATGTA24750.019.9929941
CGCAGAG234600.019.8973838
ATGGGGG161100.019.3097235
TATGTAA27600.018.9279382
TGGGGGG125350.018.9038476
TAAGTAC53100.018.6375351
Produced by FastQC (version 0.11.5)