##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921571_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6178948
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.101429078218494	31.0	30.0	34.0	26.0	34.0
2	30.088994760920468	31.0	30.0	34.0	26.0	34.0
3	30.263872749859686	31.0	30.0	34.0	26.0	34.0
4	33.93054853350441	35.0	35.0	37.0	30.0	37.0
5	33.45743353075637	35.0	35.0	37.0	28.0	37.0
6	33.35576670980238	35.0	33.0	37.0	28.0	37.0
7	33.16671980408316	35.0	33.0	37.0	27.0	37.0
8	33.064279550499535	35.0	33.0	37.0	26.0	37.0
9	34.37412129054978	37.0	34.0	39.0	27.0	39.0
10-11	34.16430410160435	37.0	33.5	39.0	26.0	39.0
12-13	33.97443448302203	37.0	33.0	39.0	25.0	39.0
14-15	34.821764481591366	38.0	33.0	40.0	25.0	41.0
16-17	34.666794493172624	38.0	33.0	40.0	25.0	41.0
18-19	34.52706965651758	38.0	33.0	40.0	24.5	41.0
20-21	34.3860069707659	38.0	33.0	40.0	23.5	41.0
22-23	34.22086308219457	38.0	33.0	40.0	23.0	41.0
24-25	34.052666813185674	38.0	33.0	40.0	22.0	41.0
26-27	33.95893297694042	38.0	32.5	40.0	21.5	41.0
28-29	33.963345054854	38.0	33.0	40.0	21.5	41.0
30-31	33.79978387906809	38.0	32.5	40.0	19.5	41.0
32-33	33.618908752752084	38.0	32.0	40.0	19.0	41.0
34-35	33.41823252113467	38.0	32.0	40.0	18.0	41.0
36-37	33.249272691726816	38.0	32.0	40.0	17.5	41.0
38-39	33.06972894091356	37.5	32.0	40.0	16.0	41.0
40-41	33.08186134597669	38.0	32.0	40.0	15.5	41.0
42-43	32.87276450619102	37.5	31.0	40.0	15.0	41.0
44-45	32.66898103042783	37.0	31.0	40.0	13.5	41.0
46-47	32.3860971964807	37.0	31.0	40.0	9.0	41.0
48-49	32.0888912643382	37.0	30.5	40.0	8.5	41.0
50-51	31.810380990421024	36.5	30.0	40.0	7.5	41.0
52-53	31.473568963519355	36.0	30.0	39.0	4.5	41.0
54-55	31.140789014570117	36.0	29.0	39.0	2.0	40.0
56-57	30.78732043059757	35.0	29.0	39.0	2.0	40.0
58-59	30.291801371366127	35.0	28.0	39.0	2.0	40.0
60-61	29.915107312765862	34.5	28.0	38.0	2.0	40.0
62-63	29.55280567177455	34.0	27.5	38.0	2.0	40.0
64-65	29.088929377622208	34.0	26.5	37.5	2.0	40.0
66-67	28.660846393269534	34.0	26.0	37.0	2.0	39.0
68-69	28.21763397264389	33.5	26.0	36.0	2.0	39.0
70-71	27.74224342072469	33.0	26.0	36.0	2.0	39.0
72-73	27.26169462827653	33.0	25.0	35.0	2.0	38.0
74-75	26.718249854182297	32.0	24.0	35.0	2.0	37.0
76-77	25.256564547880966	30.5	21.0	34.0	2.0	35.5
78-79	26.13581988390257	32.0	23.5	34.5	2.0	36.0
80-81	26.195864490201245	32.0	24.0	35.0	2.0	36.0
82-83	25.87923567248017	32.0	22.5	35.0	2.0	35.5
84-85	25.457265702834853	32.0	20.0	34.5	2.0	35.0
86-87	25.0159845171055	31.5	19.5	34.0	2.0	35.0
88-89	24.574881193368192	31.0	17.5	34.0	2.0	35.0
90-91	24.142703175362538	31.0	12.5	34.0	2.0	35.0
92-93	23.716328896116295	31.0	7.0	34.0	2.0	35.0
94-95	23.318620904399907	31.0	2.0	34.0	2.0	35.0
96-97	22.77092160348331	31.0	2.0	34.0	2.0	35.0
98-99	21.99995031516692	30.0	2.0	34.0	2.0	35.0
100-101	19.419161239097658	26.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	152269.0
3	71174.0
4	38644.0
5	27642.0
6	24429.0
7	24136.0
8	27378.0
9	31409.0
10	35476.0
11	39671.0
12	43895.0
13	48204.0
14	51902.0
15	55822.0
16	59112.0
17	60871.0
18	60118.0
19	59567.0
20	60235.0
21	62477.0
22	67622.0
23	75764.0
24	86832.0
25	102559.0
26	120589.0
27	142069.0
28	167978.0
29	196618.0
30	230110.0
31	271310.0
32	322354.0
33	392215.0
34	489246.0
35	604083.0
36	758168.0
37	779005.0
38	324583.0
39	13412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13772335048351	18.382819720076153	13.922135536029806	24.55732139341053
2	16.312453187824204	19.76687617374349	38.11631041400575	25.804360224426553
3	20.407590880055338	23.578652069990635	27.953867822981316	28.05988922697271
4	14.879850390420273	15.531883486068951	34.09472381500544	35.49354230850534
5	15.425943715751028	37.01494228408225	32.51938903595843	15.03972496420828
6	35.32091609167786	33.115450796678594	16.553018992978302	15.010614118665247
7	31.054766408846866	30.99117442435398	19.098290292871557	18.855768873927595
8	28.376167429014153	34.30816560067688	18.545693844273643	18.769973126035328
9	28.111052875030683	15.761750884409842	18.16668138320322	37.96051485735626
10-11	27.2007397695139	24.83234634746094	25.82718533060516	22.139728552420003
12-13	27.27781530147848	21.624278294231512	26.571529168604833	24.52637723568517
14-15	24.77013939123547	23.28908776705136	23.67674247550869	28.26403036620448
16-17	25.027082967246976	26.321664187401662	24.44818201179196	24.203070833559394
18-19	25.08385262927736	25.9265656823326	25.697184666901514	23.292397021488526
20-21	25.4988122746196	24.87408653635962	25.702344846793952	23.924756342226836
22-23	25.46289416409374	24.309463987447486	25.523008553930254	24.70463329452852
24-25	25.145283489459842	24.721142509166764	25.313176170300984	24.820397831072412
26-27	24.797852918435996	24.970914817048733	25.524848906826215	24.706383357689056
28-29	24.758538325667164	25.42554038724515	25.288452573346593	24.52746871374109
30-31	25.497355601464072	24.996555782004233	25.142232847268236	24.36385576926346
32-33	25.22515856844345	24.98526088377384	25.00103009888221	24.788550448900498
34-35	25.214322265463878	25.215083242373655	25.343906918599675	24.22668757356279
36-37	25.156251644274846	25.119063712462975	25.45165852185661	24.273026121405564
38-39	25.36014509500914	25.082291876209446	25.125932090128256	24.43163093865316
40-41	25.266698086475376	24.895520232956596	25.214396488362894	24.623385192205134
42-43	24.953402377221426	24.93690592432305	25.074543650075753	25.035148048379774
44-45	24.66686900079315	24.960261577558718	25.395627964194954	24.977241457453182
46-47	24.89717785165879	24.734292642466276	25.32047865246992	25.048050853405012
48-49	24.676915756752088	25.012047003340793	25.32855849867028	24.98247874123684
50-51	24.778969842454302	24.96718135156178	25.483571251673304	24.770277554310614
52-53	24.95017147131917	24.909004111637593	25.30933989227213	24.831484524771106
54-55	24.299558356128816	24.749627363742437	25.707090298605127	25.24372398152362
56-57	24.725041188587973	24.809902871167083	25.20652562546038	25.25853031478456
58-59	24.839562349412045	24.857608828564192	25.48684019179118	24.81598863023258
60-61	25.288887478652867	24.79579683470726	25.48009497723755	24.435220709402323
62-63	24.924273177894662	24.7924047543204	25.49990871021125	24.783413357573686
64-65	24.530227756441477	24.558027803932212	25.739970900189007	25.171773539437304
66-67	24.78929861313134	24.858557436040645	25.26904996148824	25.083093989339776
68-69	24.461106830341002	24.845703994127224	25.36952448899685	25.32366468653493
70-71	24.450644740187524	24.611533321768825	25.472225714149495	25.465596223894156
72-73	24.381787112245114	25.05076038974782	25.086141664681698	25.48131083332537
74-75	24.195543467340404	24.950957966133693	25.36618902007333	25.48730954645257
76-77	24.196829673601282	24.40383684863926	25.52579595442534	25.873537523334118
78-79	24.100469321762194	24.501979288168872	25.71968446703362	25.677866923035314
80-81	24.409504579837837	24.723233345440914	25.357222171504194	25.51003990321706
82-83	24.29304305468816	24.672079757946104	25.51227130922155	25.522605878144184
84-85	24.167510798342846	24.74515182431593	25.241060129559877	25.846277247781344
86-87	23.908467442406145	24.90574372229321	25.38852422676297	25.797264608537674
88-89	23.742243583303086	24.55546474009745	25.53199127705928	26.170300399540185
90-91	24.018954258760573	24.21852914380784	25.73735438806718	26.025162209364407
92-93	24.20707665423349	24.24496203185715	25.5071155376624	26.04084577624696
94-95	23.95172439241576	24.404278879409542	25.411389629142423	26.232607099032272
96-97	23.55564324187584	24.199940779363963	25.709419601350763	26.534996377409442
98-99	23.288461848151023	24.097296082126736	25.6821499145727	26.93209215514954
100-101	23.772488307476053	24.009385579564395	25.657097793968	26.561028318991557
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	184.0
1	144.5
2	139.5
3	195.0
4	195.0
5	224.5
6	366.5
7	820.5
8	1260.0
9	1302.5
10	1159.0
11	996.5
12	964.0
13	1072.0
14	1194.5
15	1288.5
16	1411.5
17	1524.0
18	1689.5
19	1825.5
20	1925.0
21	2061.5
22	2208.0
23	2425.5
24	2722.5
25	3052.0
26	3630.5
27	4575.0
28	5721.5
29	7548.0
30	10905.0
31	15796.0
32	23010.5
33	31936.5
34	41650.5
35	52976.5
36	68182.5
37	83762.5
38	97046.5
39	111046.5
40	124008.0
41	137007.5
42	152443.0
43	170760.0
44	189879.5
45	208228.0
46	229589.0
47	251913.0
48	274300.5
49	292982.5
50	304705.5
51	313541.0
52	316336.0
53	315536.5
54	312139.0
55	302957.0
56	285892.0
57	260616.5
58	233707.5
59	203597.0
60	170668.0
61	139629.0
62	111234.5
63	84607.0
64	60209.0
65	42433.0
66	30822.5
67	22215.0
68	15547.5
69	10627.5
70	7148.0
71	4807.0
72	3204.0
73	2140.0
74	1427.5
75	891.0
76	521.5
77	295.5
78	146.0
79	69.5
80	42.5
81	29.0
82	15.5
83	9.5
84	6.0
85	4.5
86	5.0
87	2.0
88	1.0
89	1.0
90	0.5
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21709197099570995
2	0.0
3	1.1328789301997687E-4
4	0.011879044782380431
5	0.013643099116548643
6	0.10534155652386133
7	0.1352819282505695
8	0.1522589282188489
9	0.10929044879484338
10-11	0.11807835249625018
12-13	0.09406941116837364
14-15	0.11682409368067187
16-17	0.07125808470956545
18-19	0.0764369598190501
20-21	0.053051101902783455
22-23	0.07906685733558529
24-25	0.07231004371617952
26-27	0.05054258427162682
28-29	0.018708686333013324
30-31	0.031218906519362197
32-33	0.03813755998593935
34-35	0.04331643509542401
36-37	0.03581515817902983
38-39	0.03731217676536524
40-41	0.02130621588011422
42-43	0.030142671535672416
44-45	0.01696081598356225
46-47	0.030725294985489437
48-49	4.126916102870586E-4
50-51	0.017122655830733646
52-53	0.008245740213382603
54-55	0.01628918061780096
56-57	0.010665245928594965
58-59	0.007404173008091345
60-61	0.030571547130676616
62-63	0.013270867468054434
64-65	0.014581770230142737
66-67	0.05763926157009251
68-69	0.07599190023932877
70-71	0.03240842939607195
72-73	0.05445910857317459
74-75	0.0464237601611148
76-77	0.19440202442228027
78-79	0.07292503513543082
80-81	0.016183984717139555
82-83	0.010592417997367837
84-85	0.024203149144482197
86-87	0.1617670192401684
88-89	0.3721750045476997
90-91	0.2727001424838014
92-93	0.274156701108344
94-95	0.0021686539520967
96-97	0.00580195852109453
98-99	0.004895655376934715
100-101	0.005186967101843226
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6178948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.33365012722894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.23077525929607	28.32663517472494
2	12.789192279224862	10.316696136002264
3	5.559613903470286	6.727185660751443
4	3.01281103077612	4.860706640591201
5	1.8937461550709496	3.819084742420836
6	1.2662446398946545	3.0643360968594
7	0.8830336679984363	2.49311797109288
8	0.6684143281232344	2.1567671720439474
9	0.4948230647762041	1.79622183326099
>10	2.856924414035332	21.48481649473376
>50	0.231597678141465	6.422467065162533
>100	0.10967136237072687	7.4562320637283825
>500	0.002626854643081695	0.6861507982310391
>1k	5.253621785856332E-4	0.3895821503964658
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.2367969434279106E-5	0.0
3	0.0	0.0	0.0	4.855195415141866E-5	0.0
4	0.0	0.0	0.0	6.473593886855821E-5	0.0
5	1.6183984717139553E-5	0.0	0.0	6.473593886855821E-5	0.0
6	1.6183984717139553E-5	0.0	0.0	6.473593886855821E-5	0.0
7	1.6183984717139553E-5	0.0	0.0	8.091992358569776E-5	0.0
8	1.6183984717139553E-5	0.0	0.0	8.091992358569776E-5	0.0
9	1.6183984717139553E-5	0.0	0.0	8.091992358569776E-5	0.0
10-11	1.6183984717139553E-5	0.0	0.0	9.710390830283732E-5	0.0
12-13	2.427597707570933E-5	0.0	0.0	1.4565586245425598E-4	0.0
14-15	3.2367969434279106E-5	0.0	0.0	1.5374785481282575E-4	0.0
16-17	3.2367969434279106E-5	0.0	0.0	1.9420781660567463E-4	0.0
18-19	3.2367969434279106E-5	0.0	0.0	2.670357478328026E-4	0.0
20-21	3.2367969434279106E-5	0.0	0.0	2.9940371726708173E-4	0.0
22-23	3.2367969434279106E-5	0.0	0.0	4.369675873627679E-4	0.0
24-25	3.2367969434279106E-5	0.0	0.0	6.87819350478431E-4	0.0
26-27	3.2367969434279106E-5	0.0	0.0	0.001043867014255501	0.0
28-29	3.2367969434279106E-5	0.0	0.0	0.0031963369816350616	0.0
30-31	3.2367969434279106E-5	0.0	0.0	0.010673337920953534	0.0
32-33	3.2367969434279106E-5	0.0	0.0	0.0271486343630016	0.0
34-35	3.2367969434279106E-5	0.0	0.0	0.04906174967000855	0.0
36-37	4.855195415141866E-5	0.0	0.0	0.08767673720510352	1.6183984717139553E-5
38-39	4.855195415141866E-5	0.0	0.0	0.15416054642311278	1.6183984717139553E-5
40-41	4.855195415141866E-5	0.0	0.0	0.23079980605112715	1.6183984717139553E-5
42-43	4.855195415141866E-5	0.0	0.0	0.30602296701639176	1.6183984717139553E-5
44-45	4.855195415141866E-5	0.0	0.0	0.39805319611040585	1.6183984717139553E-5
46-47	4.855195415141866E-5	0.0	0.0	0.507327460920532	1.6183984717139553E-5
48-49	4.855195415141866E-5	0.0	0.0	0.627347891582839	1.6183984717139553E-5
50-51	4.855195415141866E-5	0.0	8.091992358569776E-6	0.759384930897622	1.6183984717139553E-5
52-53	4.855195415141866E-5	0.0	1.6183984717139553E-5	0.8988827871670064	1.6183984717139553E-5
54-55	4.855195415141866E-5	0.0	1.6183984717139553E-5	1.0573078135630856	1.6183984717139553E-5
56-57	4.855195415141866E-5	0.0	1.6183984717139553E-5	1.2375326673731517	1.6183984717139553E-5
58-59	4.855195415141866E-5	0.0	1.6183984717139553E-5	1.4381088819650205	1.6183984717139553E-5
60-61	5.6643946509988435E-5	0.0	1.6183984717139553E-5	1.6612455712525822	1.6183984717139553E-5
62-63	6.473593886855821E-5	0.0	1.6183984717139553E-5	1.8988507428772663	1.6183984717139553E-5
64-65	6.473593886855821E-5	0.0	1.6183984717139553E-5	2.1466032729195974	1.6183984717139553E-5
66-67	6.473593886855821E-5	0.0	1.6183984717139553E-5	2.4313443000329507	1.6183984717139553E-5
68-69	6.473593886855821E-5	1.6183984717139553E-5	3.2367969434279106E-5	2.7559141135351846	4.045996179284888E-5
70-71	6.473593886855821E-5	1.6183984717139553E-5	3.2367969434279106E-5	3.1200294936937487	4.855195415141866E-5
72-73	6.473593886855821E-5	1.6183984717139553E-5	3.2367969434279106E-5	3.4900115683122754	4.855195415141866E-5
74-75	6.473593886855821E-5	1.6183984717139553E-5	3.2367969434279106E-5	3.873669110016786	4.855195415141866E-5
76-77	6.473593886855821E-5	1.6183984717139553E-5	3.2367969434279106E-5	4.290479544414357	4.855195415141866E-5
78-79	6.473593886855821E-5	2.427597707570933E-5	3.2367969434279106E-5	4.761935203209349	4.855195415141866E-5
80-81	7.282793122712799E-5	3.2367969434279106E-5	3.2367969434279106E-5	5.271229018273013	4.855195415141866E-5
82-83	8.091992358569776E-5	5.6643946509988435E-5	3.2367969434279106E-5	5.80506584616022	4.855195415141866E-5
84-85	8.091992358569776E-5	6.473593886855821E-5	3.2367969434279106E-5	6.3403268647025355	4.855195415141866E-5
86-87	8.091992358569776E-5	6.473593886855821E-5	3.2367969434279106E-5	6.899483536679707	4.855195415141866E-5
88-89	8.091992358569776E-5	7.282793122712799E-5	3.2367969434279106E-5	7.518739435903976	4.855195415141866E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	12900	0.0	38.174236	1
GGTATCA	8060	0.0	37.898296	1
TCAACGC	16925	0.0	28.453983	4
TATCAAC	17165	0.0	28.442583	2
CAACGCA	17320	0.0	27.722574	5
ATCAACG	18095	0.0	26.745419	3
AACGCAG	18630	0.0	26.024769	6
GTACATG	29125	0.0	25.909374	1
TACATGG	30335	0.0	24.6266	2
ACATGGG	31275	0.0	23.310143	3
GTAAGTA	4000	0.0	22.083746	2
CATGGGG	24730	0.0	21.451757	4
TGTAAGT	4090	0.0	21.288618	1
ACGCAGA	22285	0.0	21.244987	7
ATATGTA	2475	0.0	19.992994	1
CGCAGAG	23460	0.0	19.897383	8
ATGGGGG	16110	0.0	19.309723	5
TATGTAA	2760	0.0	18.927938	2
TGGGGGG	12535	0.0	18.903847	6
TAAGTAC	5310	0.0	18.637535	1
>>END_MODULE