FastQCFastQC Report
Mon 15 Aug 2016
SRR2921570_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921570_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8647197
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133000.040.6551861
GGTATCA80200.039.419591
TCAACGC162800.032.260574
TATCAAC169650.031.0670362
CAACGCA168950.030.9493625
ATCAACG175450.030.0922343
AACGCAG183450.028.7241236
GTACATG277950.025.8609261
TACATGG286150.024.3120962
ACGCAGA217100.023.6109227
ACATGGG304450.022.0959593
CGCAGAG226600.022.0717078
CATGGGG238900.019.7915334
GAGTACA220000.018.5327871
GCAGAGT253700.018.2836289
AGTACAT204900.017.963082
AGAGTAC205600.016.42456610-11
ATGGGGG138000.016.3149075
CAGAGTA228350.015.461328510-11
TACACTG90450.015.0366385
Produced by FastQC (version 0.11.5)