##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921570_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8647197
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.89811623350318	31.0	28.0	31.0	16.0	34.0
2	27.87819232058666	31.0	28.0	33.0	16.0	34.0
3	27.97468671061848	31.0	28.0	33.0	16.0	34.0
4	31.367204540384588	35.0	32.0	37.0	17.0	37.0
5	31.291036505818013	35.0	32.0	37.0	17.0	37.0
6	31.37477126981148	35.0	32.0	37.0	17.0	37.0
7	31.171751956154115	35.0	32.0	37.0	17.0	37.0
8	31.119039961735577	35.0	32.0	37.0	16.0	37.0
9	32.325867561476855	37.0	32.0	39.0	16.0	39.0
10-11	32.08519408081023	37.0	32.0	39.0	11.0	39.0
12-13	31.882633586351737	37.0	31.0	39.0	10.0	39.0
14-15	32.55988483898308	37.5	31.0	40.0	10.0	41.0
16-17	32.40856117884212	37.0	31.0	40.0	10.0	41.0
18-19	32.21866588676076	37.0	31.0	40.0	10.0	41.0
20-21	32.084784699596874	37.0	30.5	40.0	8.0	41.0
22-23	31.851754909712362	37.0	30.0	40.0	8.0	41.0
24-25	31.964907819262127	37.0	30.0	40.0	4.5	41.0
26-27	31.715457506056588	37.0	30.0	40.0	2.0	41.0
28-29	31.518731792510337	37.0	30.0	40.0	2.0	41.0
30-31	31.26422423358691	37.0	30.0	40.0	2.0	41.0
32-33	31.019466365806167	36.5	29.5	40.0	2.0	41.0
34-35	30.676838806841108	36.0	28.5	39.5	2.0	41.0
36-37	30.372587325118186	36.0	28.0	39.0	2.0	41.0
38-39	30.1508221103324	36.0	27.0	39.0	2.0	40.0
40-41	29.92443013614701	35.0	27.0	39.0	2.0	40.0
42-43	29.671242253414604	35.0	26.5	39.0	2.0	40.0
44-45	29.407773466939634	35.0	26.0	39.0	2.0	40.0
46-47	29.120829558988884	35.0	25.0	39.0	2.0	40.0
48-49	28.832032160247998	35.0	24.0	39.0	2.0	40.0
50-51	27.93218091365329	33.5	23.5	38.0	2.0	39.5
52-53	28.145444529597277	33.5	24.0	38.0	2.0	39.5
54-55	28.703767012593794	35.0	25.0	39.0	2.0	40.0
56-57	28.680304727647584	35.0	25.0	39.0	2.0	40.0
58-59	28.43256982580598	34.0	24.5	39.0	2.0	40.0
60-61	28.087464469700414	34.0	23.5	38.0	2.0	40.0
62-63	27.659863363816044	34.0	22.5	38.0	2.0	40.0
64-65	27.182947375895335	33.5	22.0	37.5	2.0	40.0
66-67	26.82643196402256	33.0	21.0	37.0	2.0	39.5
68-69	26.360770663603475	33.0	19.5	36.0	2.0	39.0
70-71	25.92445164600737	32.0	18.0	36.0	2.0	39.0
72-73	25.433639420959185	32.0	15.0	35.0	2.0	38.5
74-75	24.965321479318675	32.0	10.0	35.0	2.0	37.5
76-77	24.522355857048243	31.0	7.0	35.0	2.0	37.0
78-79	24.104493860843	31.0	4.0	35.0	2.0	36.0
80-81	23.640600416528038	31.0	2.0	34.0	2.0	36.0
82-83	23.082057110529576	30.0	2.0	34.0	2.0	35.0
84-85	22.643784569728204	30.0	2.0	34.0	2.0	35.0
86-87	22.129600088907424	29.5	2.0	34.0	2.0	35.0
88-89	21.601270851120887	29.0	2.0	34.0	2.0	35.0
90-91	20.989393210308496	29.0	2.0	34.0	2.0	35.0
92-93	20.424926308490484	29.0	2.0	34.0	2.0	35.0
94-95	19.73360066851721	27.0	2.0	34.0	2.0	35.0
96-97	19.08169739859055	26.5	2.0	33.0	2.0	35.0
98	18.597165301079645	25.0	2.0	33.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	573912.0
3	97108.0
4	70113.0
5	56344.0
6	51529.0
7	53753.0
8	61115.0
9	69294.0
10	73802.0
11	77437.0
12	77983.0
13	76030.0
14	74250.0
15	77678.0
16	82986.0
17	88240.0
18	91414.0
19	93590.0
20	98170.0
21	103948.0
22	112382.0
23	124495.0
24	139875.0
25	158675.0
26	180490.0
27	206147.0
28	236333.0
29	270240.0
30	310079.0
31	359061.0
32	421552.0
33	500285.0
34	606217.0
35	738970.0
36	890280.0
37	884848.0
38	429925.0
39	28646.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.79520387541635	17.21021693926897	12.907285840249468	25.087293345065216
2	15.979069622558878	19.03806510617927	39.11704442893772	25.865820842324133
3	19.890691361678684	22.926096762890413	28.330168642253735	28.85304323317717
4	13.97317997769649	15.100594478497701	35.23449658876263	35.691728955043175
5	15.108429120282254	36.45692534025141	33.43141861326233	15.003226926204007
6	34.63383435341845	33.66256584775628	16.962576387793778	14.741023411031499
7	30.315105963685625	30.94384870378929	19.937206704815406	18.80383862770968
8	27.372900969029523	34.305076998540265	19.257239674011544	19.06478235841867
9	27.327468717732355	16.010927975840207	18.68473608535868	37.97686722106876
10-11	26.29007944332428	24.890647614300608	26.90809981907114	21.911173123303975
12-13	26.797305610101045	21.965096076460092	26.989916508099558	24.247681805339305
14-15	24.08368488961079	23.573993825809744	24.215538733490657	28.12678255108881
16-17	24.3619695637436	26.636026556638136	24.863367375886853	24.138636503731412
18-19	24.41198334175039	26.22696662623009	25.986747910053783	23.374302121965737
20-21	24.960369176960263	25.341861064607475	25.925804385614303	23.77196537281796
22-23	24.841252478530972	24.93634241173073	25.714094724158166	24.508310385580128
24-25	24.55150385360987	25.19588317965936	25.705795971119	24.54681699561177
26-27	24.390466343983213	25.40287882773803	25.796426331497468	24.410228496781286
28-29	24.457133834726243	25.584483632883988	25.61505500676859	24.343327525621177
30-31	24.83873267374399	25.391144149728294	25.44390466213598	24.326218514391734
32-33	24.57309745363275	25.40940241624401	25.46537275971153	24.55212737041171
34-35	24.719142954997807	25.371710876166816	25.601933452105758	24.30721271672962
36-37	24.779616679488903	25.290859666316194	25.589509892661784	24.34001376153312
38-39	24.717236582086905	25.408830292164254	25.533420443542564	24.340512682206278
40-41	24.777933451083392	25.232316153626517	25.531936497591996	24.457813897698095
42-43	24.57937032500758	25.187312943744683	25.451061031053325	24.782255700194412
44-45	24.464970417427185	25.21619550887767	25.635774468334382	24.683059605360764
46-47	24.569562390411996	25.06958820141398	25.58689868340689	24.773950724767136
48-49	24.48424142554125	25.204201559606233	25.580711234820892	24.73084578003163
50-51	24.421982860295056	25.272941945204607	25.6957129045772	24.609362289923137
52-53	24.464814804714052	25.22911142865333	25.69258560845188	24.613488158180743
54-55	24.128391695832253	25.068414675551292	25.933681626227052	24.869512002389406
56-57	24.28866869733883	25.121867304165235	25.806225751041538	24.783238247454396
58-59	24.35886743406038	25.077819104727954	25.93001007428991	24.63330338692175
60-61	24.569301158833486	25.010853786546082	25.937675803065645	24.48216925155478
62-63	24.33011581040837	25.05087992338082	25.917938316735295	24.701065949475517
64-65	24.2196462848211	24.902099012033073	25.977141894475775	24.90111280867005
66-67	24.237311836754113	25.001836446589	25.811654269926898	24.949197446729993
68-69	24.03274375298237	24.931617576731895	25.94347269549102	25.09216597479471
70-71	24.07693160565492	24.734564632416028	25.977322191241036	25.211181570688012
72-73	24.021846544255144	24.89708591372286	25.86869345378282	25.212374088239176
74-75	23.836601570527808	24.86213170554821	26.076558810153156	25.22470791377083
76-77	23.84954300809399	24.636755003245966	26.01890811475287	25.494793873907174
78-79	23.794494667753703	24.588716493513076	26.166393728043207	25.450395110690017
80-81	23.898562087138323	24.675455676856046	26.040787667095426	25.3851945689102
82-83	23.816103970676412	24.60705513316428	26.159232434609876	25.417608461549435
84-85	23.832982262649878	24.600829328398905	26.042814429335543	25.523373979615677
86-87	23.594915616212315	24.63492745157738	26.20085245237158	25.569304479838724
88-89	23.54669409236668	24.330505584708344	26.330875407214993	25.791924915709984
90-91	23.735589319334395	24.185704326501504	26.339977708755764	25.738728645408337
92-93	23.78145433389888	24.11782594470534	26.335544739290846	25.76517498210493
94-95	23.696199822113336	24.077900698519098	26.381406002680535	25.84449347668703
96-97	23.48274062583034	23.946492800619843	26.479554036326412	26.091212537223402
98	23.443877240431693	23.827566115019696	26.36727571043027	26.361280934118337
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	164.0
2	270.5
3	412.5
4	452.0
5	524.0
6	769.5
7	1378.5
8	1981.5
9	2254.5
10	2343.5
11	2415.5
12	2657.0
13	3047.5
14	3366.0
15	3625.0
16	3784.0
17	3730.0
18	3682.5
19	3603.5
20	3445.5
21	3323.5
22	3378.0
23	3624.5
24	4083.0
25	4751.5
26	5817.5
27	7266.5
28	9550.5
29	13414.0
30	19381.0
31	28572.0
32	42509.5
33	60059.0
34	80342.5
35	104019.0
36	130000.5
37	156017.0
38	181928.0
39	207530.5
40	231915.5
41	254827.0
42	280024.5
43	307977.5
44	332158.5
45	353560.0
46	377112.5
47	398695.5
48	411658.0
49	418096.0
50	420388.0
51	420030.0
52	420137.0
53	416542.0
54	404904.5
55	386604.5
56	360447.5
57	327382.0
58	290747.0
59	250236.5
60	210125.5
61	172871.0
62	137465.5
63	104807.0
64	76350.5
65	54919.5
66	40105.5
67	29481.5
68	21232.5
69	15000.5
70	10403.0
71	7220.5
72	4991.0
73	3385.5
74	2282.0
75	1465.5
76	907.0
77	534.0
78	302.5
79	184.0
80	118.0
81	81.0
82	49.5
83	35.5
84	35.0
85	34.5
86	30.0
87	20.0
88	17.5
89	20.0
90	16.0
91	15.5
92	13.5
93	11.5
94	12.0
95	11.0
96	11.5
97	7.5
98	5.5
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.270619485134894
2	0.13321079651591147
3	0.1304815884268625
4	0.12591363421002205
5	0.1213688088752922
6	0.04584144434317849
7	0.03425387440577565
8	0.012998431746148491
9	0.008846797407298573
10-11	0.0017577950404044224
12-13	0.005666576117093204
14-15	0.010188272569712475
16-17	0.005435287295987359
18-19	0.046627826334938366
20-21	0.09353319925520374
22-23	0.09259647952972506
24-25	0.06954276628599997
26-27	0.0803034786879494
28-29	0.0971008293207614
30-31	0.07245122321140596
32-33	0.017890190312537115
34-35	0.2903368571341673
36-37	0.3770528183872762
38-39	0.34859272895020205
40-41	0.3445220456987391
42-43	0.32146833245501405
44-45	0.30544001715237895
46-47	0.3104127268061546
48-49	0.30417949307735215
50-51	0.30591994145617357
52-53	0.35063385279646114
54-55	0.337253794495488
56-57	0.32245709216524154
58-59	0.3034740621729793
60-61	0.3114708731627139
62-63	0.3110950288284169
64-65	0.3271002152489414
66-67	0.3470720049514311
68-69	0.30927362936220837
70-71	0.28632399608798087
72-73	0.2717528003583126
74-75	0.2589220530074659
76-77	0.2618883321381483
78-79	0.26780932595845797
80-81	0.2582917909699525
82-83	0.26771681043001566
84-85	0.26168017219915307
86-87	0.25671902698643273
88-89	0.2791829537363379
90-91	0.26291178517154173
92-93	0.2534347257267297
94-95	0.267907623707428
96-97	0.2669824684230046
98	0.2662365619749382
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	8647197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.56894128687252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.76886083602169	42.65964320456976
2	11.963066033913304	13.295498280990145
3	4.444242319666049	7.408855215784703
4	2.210014612234346	4.912326889215228
5	1.2044200239560032	3.3464172797972362
6	0.7541073122342307	2.5142966974527123
7	0.48624828871347636	1.8914211824452984
8	0.3754809411406618	1.669206269806806
9	0.266141039464283	1.331025821641617
>10	1.4009179361729707	14.16788172052447
>50	0.0914238134679591	3.4521816705720547
>100	0.03453113785089511	3.0476728988205153
>500	4.4081308721133925E-4	0.16354323911122265
>1k	1.0489207700561557E-4	0.1400296292681708
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1564441055292252E-5	1.1564441055292252E-5
2	0.0	0.0	0.0	2.3128882110584504E-5	1.1564441055292252E-5
3	0.0	0.0	0.0	2.3128882110584504E-5	1.1564441055292252E-5
4	0.0	0.0	0.0	2.3128882110584504E-5	1.1564441055292252E-5
5	0.0	0.0	0.0	2.3128882110584504E-5	1.1564441055292252E-5
6	0.0	0.0	0.0	2.3128882110584504E-5	1.1564441055292252E-5
7	0.0	0.0	0.0	2.3128882110584504E-5	1.1564441055292252E-5
8	0.0	0.0	0.0	2.3128882110584504E-5	2.3128882110584504E-5
9	0.0	0.0	0.0	2.3128882110584504E-5	5.7822205276461265E-5
10-11	0.0	0.0	0.0	4.047554369352288E-5	5.7822205276461265E-5
12-13	5.782220527646126E-6	0.0	0.0	5.7822205276461265E-5	1.0407996949763027E-4
14-15	1.1564441055292252E-5	0.0	0.0	6.36044258041074E-5	1.6190217477409153E-4
16-17	2.3128882110584504E-5	0.0	0.0	8.095108738704577E-5	2.197243800505528E-4
18-19	2.3128882110584504E-5	0.0	0.0	9.251552844233802E-5	2.197243800505528E-4
20-21	2.3128882110584504E-5	5.782220527646126E-6	0.0	1.3877329266350703E-4	2.3128882110584506E-4
22-23	2.3128882110584504E-5	1.1564441055292252E-5	0.0	2.7176436479936793E-4	2.7176436479936793E-4
24-25	3.469332316587676E-5	1.1564441055292252E-5	0.0	4.857065243222746E-4	2.891110263823063E-4
26-27	3.469332316587676E-5	1.1564441055292252E-5	0.0	0.0010176708128657181	3.006754674375986E-4
28-29	3.469332316587676E-5	2.3128882110584504E-5	0.0	0.003983949943548181	3.006754674375986E-4
30-31	3.469332316587676E-5	2.3128882110584504E-5	0.0	0.015253497751930481	3.2380434954818306E-4
32-33	3.469332316587676E-5	2.3128882110584504E-5	0.0	0.039949361625507086	3.5849767271405985E-4
34-35	3.469332316587676E-5	2.3128882110584504E-5	0.0	0.07310461413102998	3.816265548246443E-4
36-37	4.047554369352288E-5	4.6257764221169014E-5	0.0	0.12671158064283722	3.816265548246443E-4
38-39	4.625776422116901E-5	6.36044258041074E-5	0.0	0.21550335906537113	3.8740877535229046E-4
40-41	4.625776422116901E-5	6.938664633175351E-5	0.0	0.31487660105349746	3.931909958799366E-4
42-43	4.625776422116901E-5	6.938664633175351E-5	0.0	0.4218881563586443	3.989732164075827E-4
44-45	4.625776422116901E-5	6.938664633175351E-5	0.0	0.5516758783221892	4.0475543693522885E-4
46-47	4.625776422116901E-5	8.095108738704577E-5	0.0	0.7041819447388558	4.1631987799052106E-4
48-49	4.625776422116901E-5	8.095108738704577E-5	0.0	0.8678823900970454	4.278843190458133E-4
50-51	4.625776422116901E-5	8.67333079146919E-5	0.0	1.0458996134816867	4.3366653957345946E-4
52-53	4.625776422116901E-5	9.251552844233802E-5	0.0	1.2310347503358603	4.56795421684044E-4
54-55	4.625776422116901E-5	9.251552844233802E-5	0.0	1.4428027949403721	4.7992430379462846E-4
56-57	4.625776422116901E-5	9.251552844233802E-5	0.0	1.6872750788492503	5.030531859052129E-4
58-59	4.625776422116901E-5	9.251552844233802E-5	0.0	1.9526385255245138	5.203998474881513E-4
60-61	4.625776422116901E-5	9.251552844233802E-5	0.0	2.2383206951339263	5.203998474881513E-4
62-63	4.625776422116901E-5	9.251552844233802E-5	0.0	2.536914563181572	5.203998474881513E-4
64-65	5.203998474881513E-5	9.251552844233802E-5	0.0	2.863297783085085	5.203998474881513E-4
66-67	6.938664633175351E-5	9.251552844233802E-5	0.0	3.234897967514791	5.319642885434436E-4
68-69	6.938664633175351E-5	9.251552844233802E-5	0.0	3.643365590028769	5.49310950126382E-4
70-71	6.938664633175351E-5	9.251552844233802E-5	0.0	4.0811259417358015	5.550931706540281E-4
72-73	8.095108738704577E-5	9.829774896998415E-5	0.0	4.535672079634592	6.302620375134277E-4
74-75	8.095108738704577E-5	1.0407996949763028E-4	0.0	5.020175902087116	6.360442580410739E-4
76-77	8.095108738704577E-5	1.0407996949763028E-4	0.0	5.547017143243065	6.4182647856872E-4
78-79	8.095108738704577E-5	1.0407996949763028E-4	0.0	6.120937223935108	6.476086990963661E-4
80-81	8.095108738704577E-5	1.098621900252764E-4	0.0	6.726850330806618	6.649553606793045E-4
82-83	8.095108738704577E-5	1.1564441055292253E-4	0.0	7.35560320876233	6.823020222622429E-4
84-85	8.095108738704577E-5	1.2142663108056865E-4	0.0	8.007883942045035	6.823020222622429E-4
86	8.095108738704577E-5	1.2720885160821477E-4	0.0	8.51547617106445	6.823020222622429E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	13300	0.0	40.655186	1
GGTATCA	8020	0.0	39.41959	1
TCAACGC	16280	0.0	32.26057	4
TATCAAC	16965	0.0	31.067036	2
CAACGCA	16895	0.0	30.949362	5
ATCAACG	17545	0.0	30.092234	3
AACGCAG	18345	0.0	28.724123	6
GTACATG	27795	0.0	25.860926	1
TACATGG	28615	0.0	24.312096	2
ACGCAGA	21710	0.0	23.610922	7
ACATGGG	30445	0.0	22.095959	3
CGCAGAG	22660	0.0	22.071707	8
CATGGGG	23890	0.0	19.791533	4
GAGTACA	22000	0.0	18.532787	1
GCAGAGT	25370	0.0	18.283628	9
AGTACAT	20490	0.0	17.96308	2
AGAGTAC	20560	0.0	16.424566	10-11
ATGGGGG	13800	0.0	16.314907	5
CAGAGTA	22835	0.0	15.4613285	10-11
TACACTG	9045	0.0	15.036638	5
>>END_MODULE